Nematode Extraction Methods: Foliar Extraction Techniques Kavitha Govindasamy Jimmy R.Rich Maria L .Mendes
Jan 14, 2016
Nematode Extraction Methods:Foliar Extraction Techniques
Kavitha GovindasamyJimmy R.Rich
Maria L .Mendes
A Plant-Parasitic Nematode
Sampling for Nematodes
Sampling and extracting for nematodes serves two purposes
Diagnosis a current problem Predict a future problem
Objectives of sampling and extraction
Identify the nematode Population estimation Diagnose a nematode disease Make management decisions Regulatory purposes Research and surveys
Some Common Nematode Extraction Methods
Foliar samples Punching and incubating Scissors technique Soil samples Baermann funnel Modified Baermann funnel Root Samples Jar incubation and sieving Blending and sieving
Foliar Extraction Techniques:Using a punch or scissors
Principle Nematodes migrate out of the leaves when the lesions are soaked in water
Materials Required
Hole punch or scissorsPetri dish or other container
Cut leaf tissue and place in water
Punch the lesions and place tissue disks in water
Take samples near healthy tissue
Incubate 24 hours and observe under microscope
Acknowledgments Janete Brito, Department of Plant Industry, Gainesville,
Florida Frank Woods, Nematode Assay Lab, University of
Florida, Gainesville, Florida Joey Orajay, Entomology and Nematology Department,
University of Florida, Gainesville, FL
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