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M.Sc. Microbiology CBCS Curriculum 2011 Master of Science in Microbiology Hem. North Gujarat University, Patan M. Sc. (Microbiology) Syllabus (With effect from June 2011) Hem. North Gujarat University, Patan Page 1
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Page 1: M.Sc. Microbiology CBCS Curriculum 2010 - H.N.G.U, … · Web viewM.Sc. Microbiology CBCS Based Curriculum (With effect from June 2011) Paper No Title Credit Semester – I MB-101

M.Sc. Microbiology CBCS Curriculum 2011

Master of Science in Microbiology

Hem. North Gujarat University, Patan

M. Sc. (Microbiology) Syllabus

(With effect from June 2011)

M.Sc. Microbiology CBCS Based Curriculum(With effect from June 2011)

Paper No Title CreditSemester – I

MB-101 Cell Biology and Genetics Core 04MB-102 Diversity of Life Forms and Applied Microbiology Core 04

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M.Sc. Microbiology CBCS Curriculum 2011

MB-103 Biochemistry and Analytical Techniques Core 04Elective Any one elective paper (MBEL 1 to 6) Elective 02Practical - I Practical based on MB 101 to 103 and selected elective paper 06

Semester – II

MB-201 Molecular Genetics of Bacteria and Bacteriophages Core 04MB-202 Research Methodology &Professional Practices Core 04MB-203 Bioprocess and Biochemical Engineering Core 04Elective Any one elective paper (MBEL 7 to 12) Elective 02Practical - II Practical based on MB 201 to 203 and selected elective paper 06

Semester – IIIMB-301 Genetic Engineering and Protein engineering Core 04MB-302 Pharmaceutical Microbiology and Fermentation Technology Core 04MB-303 Omics, Inter Phase Microbiology and Recent Advances in

MicrobiologyCore 04

Elective Any one elective paper (MBEL 13 to 17) Elective 02Practical- III Practical based on MB 301 to 303 and selected elective paper 06

Semester – IV

MB 401 Project Work 18

MBEL 18 Any paper of students’ choice to develop technical skill Soft Skill 02

LIST OF ELECTIVE PAPERMB EL- 01 Advanced BioinformaticsMB EL-02 Microbial Genomics and ProteomicsMB EL-03 Nanotechnology and Systems BiologyMB EL-04 Environmentally Sound Technologies (EST)MB EL-05 Entrepreneurship and Management for Microbiology Based IndustryMB EL-06 Microbiology of Extreme Environment

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL-07 Quality Control , Assurance and Total Quality ManagementMB EL-08 Microbial Physiology and BiochemistryMB EL-09 Medical Microbiology and ImmunologyMB EL-10 Agriculture Microbiology MB EL-11 Food and Dairy MicrobiologyMB EL-12 Soil and Water Microbiology

MB EL-13 Enzyme TechnologyMB EL-14 Drug Discovery and Clinical ResearchMB EL-15 Microbial Production of Recombinant MoleculesMB EL-16 Microbial EcologyMB EL-17 Contemporary Microbiology

MB EL-18 Soft skill

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M.Sc. Microbiology CBCS Curriculum 2011

Course wise distribution of contact hours, credits and examination scheme for CBCS system

Per unit 15 hours equivalent to 1 credit per course for theory teaching. Total credits per course will be 4 equivalent to 60 hrs of direct theory teaching. For practical 2 credits per course equivalent to 4 hrs. per week, per course. (Total 6

credits). For elective paper 2 credits are allotted equivalent to 2 hrs. of counseling per week.

(Including practical if any) For soft skill paper 2 credits are allotted equivalent to 2 hrs of counseling per week.

Electives The elective course shall be offered to the students as per their choice from the list of courses provided with the curriculum. The total credits shall be 2 for each elective course including practical if any but there shall be no practical examination in the university term end examination. There shall be 2 hrs of contact teaching per week for each elective course. The question paper for end semester exam shall be of 50 marks.

Evaluation and Examination :

There shall be University examination for every core and elective course at the end of each semester.

There shall be continuous evaluation of every student for 30% of internal weightage during the semester as shown below :

Internal Assessment :

Marks for internal assessment only for core theory papers shall be awarded on the basis of attendance/regularity/punctuality, seminars, Poster presentations, viva-voce, field work/library work/academic tour, MCQ’s tests, quiz competitions, assignments etc. as determined by the Board of Studies in the respective subject. The internal assessment marks shall be notified on the department/college notice board for information of the students and it shall be communicated to the Registrar (Evaluation) before the commencement of the University examinations.

Any six head from the followings should be selected each of 5 marks in each theory paper for internal assessment. Following five activities are compulsory during each semester for internal continuous evaluation.

1. Attendance2. Assignment /Class seminar3. Individual viva voce/Group discussion

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M.Sc. Microbiology CBCS Curriculum 2011

4. Internal MCQ based test5. Internal practical test/Field work

The above five activities are to be compulsorily carried out for each semester, while out of the following three activities are to be carried out during the first three semesters respectively:

Semester I : Industrial / Educational tourSemester II : Seminar / Conference presentationSemester III: Review writing

Course contents : Each Core Course shall be divided in to FOUR units. The question paper for end semester examination comprises two sections of 35 marks each. One question is to be asked from each unit. There shall be equal weightage to each unit. Question paper shall consist 20% objective type question (MCQ/fill in the blanks/Answer in one word or one sentence) from all the units of the section. Each section is to be written in separate answer sheet.

The Elective Course shall be divided into two units. The question paper for end semester examination shall be of 50 marks & will have two sections of 25 marks each. Each section of question paper will have two questions of 10 marks each. 20% objective question shall be asked in each section. Section-I of the question paper is to be drawn from unit-I and section -II is to be drawn from unit-2. Objective questions shall be asked from respective section. Moreover, there shall at least one practical test of elective paper be conducted wherever applicable. The weightage should be given in the end semester practical examination along with the core theory paper. Out of 10 marks the internal marks should be put in the end semester practical examination.

Question Paper(a) Question paper shall consist of 2 sections.(b) Section-I covers first 2 units of the course.(c) Section –II covers remaining 2 units of the course.(d) Both sections of question paper comprises two questions with internal option of 14

marks each from each unit.(e) Third question shall be objective type of (07) marks which will cover both units.(f) Same way Section-II is to be drawn.

Practical For practical, total weightage shall be 50 marks per course i.e. total of 150 marks for 3 core courses.

The question paper will be as per the submitted format.

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M.Sc. Microbiology CBCS Curriculum 2011

Distribution of Marks for End Semester Examination :

300 Theory – Three core courses (70 + 30 =100 for each course) 50 Theory elective course 150 Practical (All core courses)

------ Total Marks : 500

Semester – IV Project Work

Students have to take up a small research project under the supervision of a teacher from the department or may carry the work in an industry / NGO / private laboratory with required facility/other university or institution. If a student is doing project out side the university then the chemicals to carry out experiment shall be replaced to the institute where he/she carry out the experimental work. All other arrangements are to be made by the student. Student has to submit the dissertation before the last date for the submission as declared by the university.

Dissertation Thesis : 250 marksPresentation : 100 marksRelated Viva : 50 marks Total (a) : 400 marks

General viva : 50 marks(all semesters)Regularly : _50 marks Total (b) : 100 marks

Grand Total (a+b) : 500 marks

There shall be an examination for the soft skill paper at the end of the semester. The total marks for the paper shall be 50.

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M.Sc. Microbiology CBCS Curriculum 2011

MB - 101 Cell Biology and Genetics 4 Credits

Section - IUnit 1 Cell Biology - I 15 hours

1.1. The Origin and evolution of cell, cell theory, Cell as experimental model1.2. Ultra-structure and function of Bacteria : Bacterial Nucleoids, Nucleosomes, Mitochondria, Microbial Cell

Surfaces, Cytoplasmic Membranes, Capsules, Organs of Locomotion , Pili or Fimbriae 1.3. Major structural and functional features of eukaryotic cell: Cell membrane and transport across the cell

membrane, Plasma membrane , Cell wall, Extracellular matrix and cell interaction, Nucleus, Endoplasmic reticulum, Golgi complex, Lysosome, Mitochondria, Chloroplast, Peroxisome, Plastids, Vacuoles, Cyto skeleton and Cell movement,

1.4. Cell singling : Singling at cell surface, Singling pathway

Unit 2 Cell Biology – II 15 hours

2.1. Cell death and Cell renewal : Programmed cell death, Stem cell and maintenance of adult tissue, Embryonic stem cell and therapeutic cloning

2.2. Eukaryotic and prokaryotic cell division and cell cycle 2.3. Host Parasite Relationship : Structures and Functions involved in Host–Parasite interaction,pathogenesis

of viral diseases, pathogenesis of bacterial diseases, Microbial mechanism for escaping host defense by bacteria or pathogens

2.4. Cancer : the development and cause of cancer, Tumor viruses, Onco genes, Tumor suppressor genes, Molecular approach to cancer treatment

Section - IIUnit - 3 Genetics - I 15 hours

3.1. The organization and sequence of cellular Genomes3.2. Mendel’s Principles, Concept of gene, Extensions and Modifications of Basic Principles, Sex Determination

and Sex-Linked Characteristics3.3. Linkage and Mapping in Eukaryotes, Prokaryotes and Bacterial viruses 3.4. DNA replication in prokaryotes and eukaryotes; DNA amplification and rearrangement

Unit - 4 Genetics – II 15 hours4.1. Gene transcription in prokaryotes and eukaryotes; post transcriptional modification4.2. Translation in prokaryotes and eukaryotes; post translational modifications; protein processing4.3. Gene Expression control in Eukaryotes4.4. Population and Evolutionary Genetics, Pedigree Analysis and Applications, Chromosome Variation

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M.Sc. Microbiology CBCS Curriculum 2011

List of Experiments

1. Simple staining and Negative staining, 2. Gram’s staining and Acid fast staining3. Special staining : Cell wall staining, Capsule staining, Spore staining, Flagella staining, Metachromatic

granule staining, Nucleus staining4. Polytene chromosome5. Mitosis and Meiosis6. Study of plant cell and animal cell 7. Demonstration of Bar body and drum stick8. Isolation of mitochondria9. Isolation of chloroplast 10. Isolation of DNA from plant animal and microbial cell 11. Enzyme Induction 12. Determination of G+C ratio

List of Reference Books

1. Alexoplous, Introductory mycology2. Biswas, Viruses3. Dubey, Introduction to Fungi4. Flint, Virology5. Luria, Virology 6. Prescott, Microbiology 7. R. M. Atlas, Principles of Microbiology8. Tortora, Microbiology 9. Perry, Microbial life 10. Carter, Virology Principle and application

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M.Sc. Microbiology CBCS Curriculum 2011

MB - 102 Diversity of Life forms and Applied Microbiology 4 Credits

Section - IUnit - 1 Concept of Biodiversity 15 hours

1.1. Biodiversity Basics; Biodiversity: Origin, speciation and extinction and ecological role of biodiversity; Types of biodiversity: alpha, beta, and gamma diversity

1.2. Techniques for molecular identification: Morphological methods, Biochemical, serological, Molecular methods, Fatty acid profiling, metabolic fingerprinting ( Biolog ), DGGE and TGGE

1.3. Taxonomy methods: Taxonomy and classification: Taxonomic Hierarchies; Classical approach to classification; Numerical taxonomy; Molecular taxonomy; modern methods of taxonomy and systematic.

1.4. Overview of plant and Animal biodiversity: Taxonomic criteria of classification; Classification system; Distinguishing and significant characteristics of representative group

Unit - 2 Eukaryotic Microorganism and Archaebacteria 15 hours

2.1. Mycology : Taxonomic criteria of fungal classification; Distinguishing and significant characteristics of Mastigomycotina, Ascomycotina, Basidiomycotina, Zygomycotina and Deuteromycotina; Fungal growth and differentiation;

2.2. Protozoa: Structure, classification, reproductive strategies and economic importance; Algae : structure, classification, reproductive strategies and economic importance; algal ecology ; lichens

2.3. Archaebacteria : Phenotypes of Archea : Methanogenic bacteria, extremophiles : thermophilic, halophilic bacteria; Cell structure & composition; Eco-physiology; Taxonomy & nomenclature; Salient features of representative group

2.4. Virology : Structure, classification, cultivation and economic importance of plant viruses and animal viruses; Structure, classification, cultivation and economic importance of Bacteriophage; Viroids; Prions

Section – II

Unit - 3 Prokaryotic Microorganismsand Viruses 15 hours

3.1. Microbial taxonomy : Microbial evolution and diversity, Taxonomic ranks, Classification system, Major characteristics used in taxonomy, Assessing Microbial Phylogeny, The Major Divisions of Life, Bergey’s Manual of Systematic Bacteriology, A Survey of Procaryotic Phylogeny and Diversity

3.2. Overview of the Deinococci and Nonproteobacteria Gram Negatives3.3. Overview Proteobacteria3.4. Overview of The Low G+C and High G+C Gram Positives bacteria

Unit - 4 Applied Microbiology 15 hours

4.1. Application of Microbiology and Role of Microbiologist in Dairy and Food industry4.2. Application of Microbiology and Role of Microbiologist in Clinical and Diagnostic field 4.3. Application of Microbiology and Role of Microbiologist in Agriculture 4.4. Application of Microbiology and Role of Microbiologist in Environment science

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M.Sc. Microbiology CBCS Curriculum 2011

List of Experiments

1. Isolation and identification of bacteria belonging to Enterobacteriaceae, Bacillaceae, PseudomonadaceaeAzotobactereceaefamily

2. Isolation and identification of industrially important molds and fungi.3. Isolation of actinomyctes4. Isolation of bacteriophage5. Screening of Extremophiles (Acidophiles / Alkaliphiles / Halophiles/ Psychrophiles / Thermophiles) 6. Study of Protozoan and algal permanent slide 7. Growth curve of E. coli and determination of growth rate and generation time, Effect of pH, temperature, salt

and glucose on growth8. Identification of Microorganism with 16S rRNA homology technique9. Microbiological analysis of clinical sample 10. Identification of bacteria by rapid kit11. Microbiological examination of food12. Microbiological examination of water13. Microbiological analysis of Air14. Microbiological analysis of Milk

List of Reference Books

1. Pollard, Cell Biology.2. Alberts, Molecular Biology of Cell. 3. Coopper, The cell.4. Prescott, Microbiology.5. Lodish, Molecular Cell Biology. 6. Griffth, An Introduction to Genetic analysis.7. Moat, Microbial Physiology.8. David White, The Physiology and Biochemistry of Prokaryotes9. Pierce Genetics A Conceptual Approach. 10. Benjamin, Gene VIII. 11. Syndeer and Champness. Molecular genetics of bacteria12. Nancy, Fundamental Bacterial Genetics 13. Tamarin, Principles of Genetics. 14. J. D. Watson, Molecular Biology of the Genes.15. Prescott, Microbiology16. Griffith, Genetic Analysis

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M.Sc. Microbiology CBCS Curriculum 2011

MB - 103 Biochemistry and Analytical Techniques 4 Credits

Section - IUnit - 1 Biochemistry - I 15 hours

1.1. Basics of Chemistry for biologist 1.2. Protein Structure and Function : Amino Acids,Structure of Proteins, enzyme 1.3. Intermediary Metabolism: Bioenergetics and Oxidative Phosphorylation, Introduction to Carbohydrates,

Glycolysis, TCA Cycle, Gluconeogenesis, Glycogen Metabolism, Metabolism of Monosaccharides and Disaccharides, PPP Pathway and NADPH, Glycosaminoglycans and Glycoproteins

1.4. Lipid Metabolism: Metabolism of Dietary Lipids, Fatty Acid and Triacylglycerol Metabolism, Complex Lipid Metabolism, Cholesterol and Steroid Metabolism

Unit - 2 Biochemistry - II 15 hours

2.1. Nitrogen Metabolism: Amino Acids: Disposal of Nitrogen, Amino Acid Degradation and Synthesis, : Conversion of Amino Acids to Specialized Products,

2.2. Integration of Metabolism : Metabolic Effects of Insulin and Glucagon, The Feed/Fast Cycle, Diabetes Mellitus, Obesity, Nutrition, Vitamins

2.3. Nucleotide metabolism:Characteristics and types of Nucleic acids, Biosynthesis and catabolism of purines and pyrimidines,

2.4. Molecular biology techniques: Principle and technique of nucleic acid hybridization and cot curve; sequencing of proteins and nucleic acids; Southern , Northern and South-Western blotting techniques; Polymerase Chain Reaction; Methods for measuring nucleic acid and protein interactions

Section - IIUnit - 3 Analytical Technique - I 15 hours

3.1. Electrochemistry: pH and buffers, Potentiometric and Conductometric titration, Agarose electrophoresis, Native PAGE, SDS PAGE, isoelectric focusing (IEF), two dimensional gel electrophoresis, gels

3.2. Purification and Identification of Bio molecules : Introduction to Chromatography, Column Chromatography, Ion-Exchange Chromatography, Gel Exclusion Chromatography, Affinity Chromatography and Immunoadsorption, Perfusion Chromatography, GC. HPLC, FPLC.

3.3. Histochemical and immunotechniques: Antibody generation, detection of molecules using ELISA, RIA, western blot, immunoprecipitation, flowcytometry and immunofluorescence microscopy, detection of molecules in living cells, in situ localization by techniques such as FISH and GISH.

3.4. Radiolabeling techniques: Properties of different types of radioisotopes normally used in biology, their detection and measurement,Autoradiography

Unit - 4 Analytical Technique – II 15 hours

4.1. Biophysical methods: Analysis of biomolecules using UV/visible, IR, Fluorescence, CD, ESR spectroscopy, structure determination using X-ray diffraction and NMR; analysis using light scattering, different types of mass spectrometry and surface plasma resonance methods, MALDI TOF, Atomic spectroscopy

4.2. Microscopic techniques: Light, Phase Contrast, Fuorescence, Scanning and Transmission Electron Microscopy and other Advanced Microscopy

4.3. Electrophysiological methods: Single neuron recording, patch-clamp recording, ECG, Brain activity recording, lesion and stimulation of brain, pharmacological testing, PET, MRI, fMRI, CAT.

4.4. Centrifugation technique, Biosensors

List of Experiments

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M.Sc. Microbiology CBCS Curriculum 2011

1. Estimation of carbohydrates ( Coles, DNS and Anthron method)2. Estimation proteins ( Folin, Bradford and Biurates) 3. Estimation of lipid4. Estimation of inorganic phosphorus5. Estimation of inorganic nitrogen6. Determination of Km and V max for Enzyme 7. Enzyme inhibition study 8. Agarose gel electrophoresis for DNA9. SDS PAGE analysis for Protein 10. 2D gel electrophoresis technique ( Demonstration) 11. Determination of pK value 12. Separation of amino acid by paper chromatography13. Separation of sugars/ fatty acid by thin layer chromatography14. Determination of molecular weight of protein by Column chromatography

List of Reference Books

1. Lehninger, Principles of Biochemistry .2. Lippincotts, Illustrated Reviews Biochemistry3. David Holme, Analytical Biochemistry4. Rodney Boyer, Modern Experimental Biochemistry5. Hames and Hooper, Instant Notes in Biochemistry6. Wilson and Walker, Principles and Techniques of Biochemistry and Molecular Biology7. Debnath , Tools And Techniques Of Biotechnology8. Voet and Voet, Bichemistery

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M.Sc. Microbiology CBCS Curriculum 2011

MB 201 Molecular Genetics of Bacteria and Bacteriophages 4 Credits

Section - I

Unit - 1 DNA Damage, Repair, Recombination and Gene Regulation 15 hours

1.1. Mutations and Mutagenesis: Biochemical Basis of Mutation, Spontaneous Mutation, Fluctuation Test, Useful Phenotypes in bacterial genetics, Mutation Rate, Expression and Selection of Mutant Cell, Types of Mutation, Mutagens, Reversion and suppression of mutation

1.2. DNA Repair Evidences of DNA Repair, Specific repair pathway, General repair mechanisms, Repair pathway in E. coli, Bacteria phage repair pathway

1.3. Recombination: Overview of recombination, Molecular Model Recombination, Molecular basis for recombination in E. coli, Phage recombination , illegimate recombination, Genetic and Functional Analysis of Recombination

1.4. Regulation of gene expression: Overview of gene regulation, Transcriptional regulation in bacteria, Negative regulation, Positive regulation, Regulation by attenuation, Feedback inhibition, Translational control.

Unit-2 Plasmid, Transposition and Conjugation 15 hours

2.1. Plasmid Molecular Biology: What is plasmid, Types and properties of plasmid: Replication, Control of Copy Number, Partitioning, Incompatibility, Maintaining Plasmid belonging to the same incompatibility group. Conjugal Functions, Pilus Production, Transfer DNA Replication

2.2. Properties of Particular Plasmids :Major Chromosome-Mobilizing other than F, Bacteriocins, Resistance Plasmids, Agrobacatrium plasmid Ti, Broad Host Range Plasmids

2.3. Conjugation: Discovery of Conjugation, Mechanism of DNA transfer during conjugation in Gram Negative Bacteria, Chromosome transfer by Plasmid, Genetic mapping with Hfr crosses, Chromosome mobilization, Prime factors, Transfer systems of gram Positive bacteria.

2.4. Transposition and site specific recombination : Insertion sequences, Detection of transposition in bacteria, Types of bacterial Transposon, Transposition, Transposon Mutagenesis, Site specific recombination, Phage Mu

Section - II

Unit-3 Bacteriophage Genetics and Transformation 15 hours

3.1. Genetics of intemperate bacteriophages:T4 Bacteriophage as a Model Genetic System,Morphology and Composition, Experimental Methods Used to Study Phage Infection, Genetic Organization of T4, DNA Replication, Molecular Biology of T4 Phage Infection, Morphogenesis and Maturation;Properties of some other common intemperate bacteriophages.

3.2. Genetics of Temperate Bacteriophages:Overview of various temperate bacteriophages, General Nature of the Temperate Response, Bacteriophage Lambda as the Archetypal Temperate Phage, Genetic Map of Lambda. Lytic Cycle, Lysogeny Cycle, Lysogenic phage and bacatrial pathogenesis.

3.3. Transduction: Bacteriophage Lambda: A Specialized Transducing Phage, Production of Transducing Particles, Physiology and Genetic Consequences of Transduction, Specialized Transducing Phages Other than Lambda, Generalized Transduction, Cotransduction.

3.4. Genetic Transformation:Standard Genetic Transformation Systems¸ Discovery of Genetic Transformation, Competent Cells, DNA Uptake and Entry, Establishment of Donor DNA in the Recipient Cell, Other Transformation Systems, Transfection, Genetic Mapping Using Transformation

Unit- 4 Advances in Microbial Genetics 15 hours

4.1. Bacterial and Bacteriophage Evolution : What Is Evolution, Expression of Evolutionary Relationships, Specific Examples of Evolution, Genetic Structure of the Chromosome, Bacteria, Bacteriophage ;

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M.Sc. Microbiology CBCS Curriculum 2011

4.2. Advanced Regulatory Topics : Global Regulatory Mechanism: Catabolic sensitive Operon, Regulation of Nitrogen assimilation, Porin synthesis, Heat shock protein, Virulence genes, Endospore formation, Ribosome and t RNA synthesis; Two component regulation system; Inteins, Small RNA Molecules

4.3. Strain Construction: Construction of Bacterial strains, Operon and Gene fusion, Construction of Phage Mutants

4.4. Genetic Methods for Investigating Bacteria: Metabolic pathways, Microbial physiology¸ Bacterial virulence, Specific mutagenesis, Taxonomy, evolution and epidemiology

List of Experiments

1. Ultraviolet irradiation survival curve2. Fluctuation test, rapid - plate technology.3. Isolation of auxotrophic mutant4. Isolation Respiratory deficient mutant5. Isolation of temperature sensitive mutant 6. Isolation of streptomycin resistant mutant by gradient plate technique7. Ames test8. Conjugation in E. coli9. Transduction 10. Plasmid curring11. Phage titration

List of Reference Books

1. Trun and Trempy, Fundamental Bacterial genetics2. Syndeer and Champness, Molecular genetics of bacteria3. Maloy, Microbial genetics. 4. Edward,Bacterialand Bacteriophage Genetics.5. Dale, Molecular Genetics of Bacteria. 6. Streips, Modern Microbial genetics. 7. Alan, Principles of Molecular Virology

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M.Sc. Microbiology CBCS Curriculum 2011

MB - 202 Research Methodology and Professional Practices 4 Credits

Section - IUnit -1 Introduction to Scientific Research 15 hours

1.1. Characteristics and types of scientific research, Basics of research methodology, Research and Experimental design, Method of Data collection

1.2. Scientific Deliveries and Communications: Writing Research proposal, Paper, Thesis, Report and Citations, Presenting scientific research: Power point presentations, Posters, Flyers, etc.

1.3. Publication processes, Review Processes and Significance of scientific communications1.4. Study of Critical review and scientific Paper in Microbiology

Unit-2 Statistical Methods and Data analysis 15 hours

2.1. Definition and scope, Organizing a statistical survey and presentation of statistically analyzed information, Basic statistical methods: Measures of central tendency, dispersion and standard error; Probability distributions: binomial, poisson and normal distribution

2.2. Statistical significance: Hypothesis testing, types of error, level of significance, Student’s t test, F test and Chi square goodness of fit

2.3. Comparing Parametric and Non parametric statistics, Rank test, F-max test, Mann –Whitney (U) test, and Sign test, Applications of non parametric statistics in biological research

2.4. Simple linear regression and correlation analysis,

Section – II

Unit -3 Computer Science and Bioinformatics 15 hours

3.1. Basic computing: MS Office ®, Internet, , Use of computers in statistical analysis, Database and Data base management system, Biological database : Sequence, Structure and classification

3.2. Sequence Analysis : concepts of sequence similarity, identity and homology, Global and Local alignment, Scoring matrices, BLAST, FASTA

3.3. Multiple Sequence Alignments (MSA): The need for MSA, basic concepts of various approaches for MSA (e.g. progressive, hierarchical etc.); Introduction to CLUSTALW and PileUp ; concept of dendrogram and its interpretation.

3.4. Application of Bioinformatics: Gene finding, PCR Primer designing, Microbial identification, Comparative genomics, Secondary and tertiary protein structure prediction

Unit - 4 Professional Practices 15 hours4.1. Concept of Quality Control and Assurance in life science research and industry, Concept of GMP, GLP,

ISO, WHO 4.2. The Business of biotechnology: Sciences and Business, Biotechnology company fundamentals, funding,

research development and marketing4.3. Legal and regulatory issues for Biotechnology based business 4.4. Survey on Microbiology and Biotech Industry in State and Nation

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M.Sc. Microbiology CBCS Curriculum 2011

List of Experiments

1. Standard operation procedure and validation of autoclave, pH meter, UV visible spectrophotometer and laminar air flow

2. Computation of Mean, Mode and Median3. Computation of Standard deviation and Co-efficient of variation.4. Calculation of confidence limit for the population mean.5. Student's 't' test. (Paired and unpaired)6. ANOVA.7. regression and correlation analysis8. Chi square goodness of fit9. perform non parametric tests10. Application statistical software ( SPSS/ minitab/ metlab)11. Use of Word, Excel, Power Point, Access and internet12. Submission of scientific Review in relevant topics 13. Internet gene bank search and SRS14. BLAST and FASTA15. Local Global and Multiple sequence alignment 16. Protein structure visualization17. Primer designing

List of Reference Books

1. Yali Friedman, Building Biotechnology2. David Hoyle, ISO 9000 Quality Systems Handbook3. Denyer, Handbook of Microbiological Quality Control4. The North Carolina Association for Biomedical Research, Mapping your future: Exploring Careers In

biomanufacturing5. Chap, Introductory Biostatistics6. Zar, Biostatistical Analysis.7. Gibas, Developing Bioinformatics computer skill8. Ghosh, Bioinformatics Principle and application 9. Selzer, Applied Bioinformatics10. Baxevanis, Bioinformatics 11. Claverie, Bioinformatics for dummies12. Mount, Bioinformatics: sequence and genome analysis13. Oren, Bioinformatics, Gene, Proteins and Computers.14. Rastogi, Bioinformatics15. Twyman, Instant notes on Bioinformatics,16. Jin, Essential Bioinformatics

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M.Sc. Microbiology CBCS Curriculum 2011

MB – 203 Bioprocess and Biochemical Engineering 4 Credits

Section - I

Unit -1 Bioprocess Microbiology – I 15 hours

1.1. Introduction: Scope of Biotechnology and Industrial Microbiology : Nature of Biotechnology and Industrial Microbiology; Characteristics of Industrial Microbiology; Patents and Intellectual Property Rights in Industrial Microbiology and Biotechnology; The Use of the Word ‘Fermentation’ in Industrial Microbiology; Organizational Set-up in an Industrial Microbiology Establishment

1.2. Screening for Productive Strains and Strain Improvement in Biotechnological Organisms: Sources of Microorganisms used in Biotechnology; Selection from naturally occurring variants; Manipulation of the genome of industrial organisms in strain improvement

1.3. Metabolic Pathways for the Biosynthesis of Industrial Microbiology Products: The Nature of Metabolic Pathways; Industrial Microbiological Products as Primary and Secondary Metabolites; Trophophase-idiophase Relationships in the Production of Secondary Products; Role of Secondary Metabolites in the Physiology of Organisms Producing Them; Pathways for the Synthesis of Primary and Secondary Metabolites of Industrial Importance; Carbon Pathways for the Formation of Some Industrial Products Derived from Primary Metabolism; Carbon Pathways for the Formation of Some Products of Microbial Secondary Metabolism of Industrial Importance.

1.4. Overproduction of Metabolites of Industrial Microorganisms: Mechanisms Enabling Microorganisms to Avoid Overproduction of Primary Metabolic Products Through Enzyme Regulation; Derangement or Bypassing of Regulatory Mechanisms for the Over-production of Primary Metabolites; Regulation of Overproduction in Secondary Metabolites; Empirical Methods Employed to Disorganize Regulatory Mechanisms in Secondary Metabolite Production

Unit -2 Bioprocess Microbiology – II 15 hours

2.1. Growth Kinetics : Introduction; Kinetics of batch culture; Disadvantages of batch culture ; Advantages of continuous culture ; Growth kinetics for continuous culture; Material balance for CSTR: Rate of product formation, Growth kinetics, biomass and product yields, YX/S and YP/S, Biomass balances (cells) in a bioreactor, Material balance in terms of substrate in a chemostat, Modified chemostat, Fed batch culture

2.2. Industrial Media and the Nutrition of Industrial Organisms : The Basic Nutrient Requirements of Industrial Media; Criteria for the Choice of Raw Materials Used in Industrial Media; Some Raw Materials Used in Compounding Industrial media; Growth Factors; Water; Some Potential Sources of Components of Industrial media, Carbohydrate sources, Protein sources; The use of plant waste materials in Industrial Microbiology media: Saccharification of Polysaccharides, Starch, Cellulose, hemi-celluloses and lignin in plant materials

2.3. Sterility in Industrial Microbiology : Introduction; The basis of loss by contaminants; Physical and Chemical Methods of Achieving Sterility : Hot plates; High temperature sterilization; Dry heat sterilization; Sterilisation with filtration; Microwave sterilization; Electron beam sterilization; Chemical sterilization; Batch sterilization; Continuous sterilization; The sterilization of the fermentor and its accessories; Media sterilization; Viruses (Phages) in Industrial Microbiology

2.4. Bioprocess Scale-up: Introduction; Scale-up procedure from laboratory scale to plant scale( Scale-up for constant KLa, for shear forces, constant mixing time); Bioreactor design criteria ; CSTR chemostat versus tubular plug flow; Dynamic model and oxygen transfer rate in activated sludge; Aerobic wastewater treatment; Fermentation economics

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M.Sc. Microbiology CBCS Curriculum 2011

Section II

Unit- 3 Biochemical Engineering 15 hours

3.1. Bioreactor : Introduction; Background; Bioreactor for batch type fermentation : The Aerated Stirred Tank, Anerobic Batch, Airlift bioreactors, Bubble column, Surface or Solid State; Bioreactor Configurations for Fed-batch Cultivation and Continuous fermentations; Calculation for bioreactor: Heat transfer; Design equations for CSTR fermenter; Monod model for a chemostat ; Temperature effect on rate constant; Scale-up of stirred-tank bioreactor

3.2. Dissolved Oxygen Measurement and Mixing: Introduction; Measurement of dissolved oxygen concentrations; Oxygen transfer rate (OTR); Respiration quotient (RQ); Agitation rate studies ; Gas and Liquid System (Aeration and Agitation): Introduction; Aeration and agitation; Effect of agitation on dissolved oxygen; Air sparger; Oxygen transfer rate in a fermenter; Mass transfer in a gas–liquid system; Gas hold-up; Agitated system and mixing phenomena; Characterisation of agitation; Types of agitator; Gas–liquid phase mass transfer (Oxygen transport, Diameter of gas bubble formed D0 )

3.3. Material and Elemental Balance: Introduction; Growth of stoichiometry and elemental balances; Energy balance with example of continuous ethanol fermentation; Conservation of mass principle with example of Acetic acid fermentation process

3.4. Fermentation Process Control: Introduction; Bioreactor controlling probes; Characteristics of bioreactor sensors; Temperature measurement and control; DO measurement and control; pH/Redox measurement and control; Detection and prevention of the foam; Biosensors

Unit - 4 Downstream Processing, Scale up and Economics 15 hours

4.1. Extraction of Fermentation Products: Solids (Insolubles) Removal: Filtration, Centrifugation, Coagulation and flocculation, Foam fractionation, Whole-broth treatment; Primary Product Isolation: Cell disruption, Liquid extraction (Continuous extraction column process, rotating disk contactors), Dissociation extraction, Adsorption ( Ion-exchange, Langmuir isotherm, Freundlich isotherm and Fixed-bed), Precipitation; Purification: Chromatography, Carbon decolorization, Crystallization; Product Isolation: Crystalline processing, Drying

4.2. Membrane Separation Processes: Introduction; Types of membrane; Membrane processes; Nature of synthetic membranes; General membrane equation; Cross-flow microfiltration; Ultrafiltration; Reverse osmosis; Membrane modules.

4.3. Advanced Downstream Processing in Biotechnology: Introduction; Protein products; Cell disruption; Protein purification; General problems associated with conventional techniques; Fluidised bed adsorption; Design and operation of liquid fluidised beds; Interfaced and integrated fluidised bed/expanded bed system

4.4. Immobilized Enzymes and Immobilized Cells: Advantages of immobilized biocatalysts in general; Methods of immobilizing enzymes; Methods for the immobilization of cells; Practical Application of Immobilized Biological Catalyst Systems; Bioreactors Designs for Usage in Biocatalysis ; Immobilised cell reactor(ICR) experiments and ICR rate model

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M.Sc. Microbiology CBCS Curriculum 2011

List of Experiments

1. Amylase Production: Screening, Optimization, production and Purification (affinity chromatography)2. Protease Production: Screening, Optimization, Production and Purification (Salt precipitation, ion exchange

chromatography)3. Immobilization of enzyme 4. Estimation aeration efficiency5. Reverse micelle formation 6. Screening of Organic acid producer microorganism7. Screening of Antibiotic producer microorganism 8. Operation of fermentation 9. Sterility testing

List of Reference Books

1. Okafor, Modern Industrial Microbiology and biotechnology.2. Najafpour, Biochemical Engineering and Biotechnology.3. Shigeo, Biochemical engineering.4. Whittaker, Principles of fermentation technology.5. Alexander, Microbial Biotechnology.6. Sikyta, Techniques in Applied Microbiology.7. Vogel, Fermentation and Biochemical Engineering Handbook.8. Mcneil, Practical Fermentation Technology.9. Doran, Bioprocess engineering Principle.10. Nathan, Modern Biotechnology.11. Mansi, Fermentation microbiology and Biotechnology, Taylor and Francis12. Waites, Industrial Microbiology: An Introduction, Blackwell publication 13. Michal, Bioprocess Engineering Basic Concept, Prentica Hall of India14. Crueger, A text book of Industrial microbiology.15. Volkmar, Microbial Fundamentals of Biotechnology16. Peppler, Microbial technology: fermentation technology

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M.Sc. Microbiology CBCS Curriculum 2011

MB-301 Genetic Engineering and Protein Engineering 4 CreditsSection - I

Unit - 1 Concepts and tools and technique for genetic engineering 15 hours1.1. Concepts and application: Introduction to gene cloning; Application of Recombinant microorganisms; Application of transgenic Plant Technology; Application of animal cloning and Transgenic animal technology1.2. Enzyme used in genetic engineering: Restriction endonuclease; DNA Polymerase: Reverse transcriptase;

RNA polymerase; Alkaline Phosphatase; Polynucleotide Kinase; DNA ligase; Deoxyribonuclease; Ribonuclease; Phosphodiesterase; β Agarase; Uracil – DNA Glycoylase; Proteinase K; Lysosome; Topoisomerase

1.3. Cutting of DNA: Host Controlled Restriction Modification system; Nomenclature of Restriction Endonuclease; Types of Restriction Endonuclease; Recognition sites; Cleavage by Restriction endonuclease; variants of Restriction Endonuclease; Application of Restriction Endonuclease

1.4. Joining of DNA Fragments: Introduction; Ligation of DNA fragment using DNA ligase; ligation using homopolymer Tailing; Increasing versatility and Efficiency of ligation by modification of the Ends of Restriction Fragments; Ligation of PCR products

Unit - 2 Vectors 15 hours

2.1 Plasmid as a vector: pSC101; pSF124; Col E1; pBR 322 series; pUCSeries; pGEM series; pET,pBAD,2.2 Bacteriophage as a vector:lambda phage; M13; Cosmid;Phagemids; Phasmids;Fosmid; 2.3 Advanced vector: Shuttle vector; Expression vector; Advanced gene trapping vector; Specialized vector for

making SS DNA; facilitate Purification of cloned product; promotes solubilization of expressed product; promotes export of cloned product; PAC, YAC, BAC, HAC;

2.4 Other vectors : Chimeric vector; Gram negative bacteria other than E. coli as cloning vector; Gram positive bacteria as cloning vector; Plant and Animal Vectors; Fungi system other than yeast.

Section - II

Unit - 3 15 hours1. Introduction of DNA in to Host : Introduction; Introduction of DNA in to bacterial cells; Introduction of DNA in

to yeast cells; Genetic transformation of Plants; Introduction DNA in to insects2. Construction of Genomic and c DNA Libraries: Introduction; Genomic Library ; cDNA Library; PCR as an

alternative to library Construction; Functional cloning; Positional cloning; Differential cloning 3. Techniques for Selection, Screening and characterization of trans formants: Introduction; Selectable Marker

gene; Reporter genes; Screening of clone(s) of interest; Nucleic Acid Blotting and Hybridization; Protein structure/ Function Fusion- based techniques

4. Safety regulation related to genetic engineering: Introduction; National regulatory Mechanism for implementation of biosafety guideline for handling GMOs; Salient features , revised Guidelines for Research in transgenic Plant and risk assessment; regulation of Gm Plant; Regulation of steam Cell research and human cloning; Patenting for Molecular Biotechnology; Ethical issues

Unit – 4 15 hours

1. Site directed mutagenesis; Concept tools, technique of and application 2. Concept of protein engineering; Evolutionary Methods for Protein Engineering; Phage Display Systems for

Protein Engineering; Cell Surface Display Systems for Protein Engineering; Cell-Free Display Systems for Protein Engineering;

3. Protein engineering in basic and applied biotechnology; Enhanced recovery and folding of recombinant proteins using Fusion protein strategies; Protein engineering for affinity purification; Stabilization of industrial enzymes by protein engineering; Engineering of Therapeutic Proteins

4. DNA Microarray technology: Concepts, tools and techniques, data generation and analysis, application; Microarrays for Bacterial Typing; Overview of protein Microarray technology

List of Experiments

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M.Sc. Microbiology CBCS Curriculum 2011

1. Isolation of genomic DNA from Bacteria2. Isolation of genomic DNA from Plant3. Isolation of genomic DNA from Blood4. Isolation of genomic from fungi5. Agarose gel electrophoresis and recovery of DNA from gel 6. Isolation of plasmid7. RFLP8. RAPD9. PCR amplification 10. Cloning in bacteria11. Transformation of plants 12. Protein denaturation and in vitro Protein folding13. BT cotton testing

List of Reference Books

1. Nicholl, An Introduction to Genetic Engineering2. Reece, Analysis of Genes and Genomes3. Primrose, Principle of gene Manipulation 4. Brown, Gene cloning and DNA Analysis 5. Howe , Gene Cloning and Manipulation6. Wong , The ABC of gene cloning 7. Watson, Recombinant DNA genes and genomics 8. Budisa, Engineering the Genetic Code9. Sheldon J. Park, Protein Engineering and Design 10. Allan Svendsen Enzyme Functionality Design, Engineering, and Screening 11. Lilia Alberghina Protein engineering in industrial Biotechnology by Lilia Alberghina12. Joanna S. Albala, Protein Arrays, Biochips, and Proteomics The Next Phase of Genomic 13. Isaac, Discovery by Microarrays for an Integrative Genomics

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M.Sc. Microbiology CBCS Curriculum 2011

MB - 302 Pharmaceutical Microbiology and Fermentation Technology 4 Credits

Section - IUnit - 1 Pharmaceutical Microbiology 15 hours

1. Microbial aspect of Pharmaceutical processing : Microbial spoilage and prevention of pharmaceutical product; Principle and practice of sterilization, Sterile pharmaceutical product , Factory and hospital hygeny and good Manufacturing practice

2. Drug Discovery: Targets and Receptors; Drug Discovery: Small Molecule Drugs; Drug Discovery: Large Molecule Drugs: Enzymes, Vaccines (Attenuated, DNA, Subunit, vector), Antibodies( Monoclonal antibodies) , Cytokines, Hormones, Gene Therapy, Stem Cells;

3. Drug Development and Preclinical Studies; Clinical Trial : Overview, Role of microbiologist in CRO; 4. Bio safety: Principle of bio safety , Laboratory Bio safety Level Criteria; Bioterrorism; Biomedical waste

management Unit - 2 Novel Microbial products 15 hours

1. Probiotics and Prebiotic : Concept, methods and application 2. Production of Antibiotics and Anti-Tumor Agents: Classification and Nomenclature of Antibiotics; General

production methods; Penicillin, Cephalosporins, Tetrcycline, Bacitracin production; Problem of Antibiotic Resistance; The Search for New Antibiotics; Combating Resistance and Expanding the Effectiveness of Existing Antibiotics; Anti-Tumor Antibiotics; Newer Methods for Searching for Antibiotic and Anti-tumor Drugs; Synthesis of commercial products by recombinant microorganism: Small Biological molecules, Antibiotics, Biopolymers;

3. Biocatalysis in Organic Chemistry: Nature and Use of Steroids and Sterols, Uses of Steroids and Sterols, Types of microbial transformations in steroids and sterols, Fermentation conditions used in steroid transformation, Asymmetric Catalysis in the Pharmaceutical and Agrochemical Industries

4. Production of Microbial Insecticides: Alternatives to Chemical Insecticides; Biological Control of Insects; Bacillus thuringiensis Insecticidal toxin ; Production of Biological Insecticides; Bioassay of Biological Insecticides; Formulation and Use of Bioinsecticides; Safety Testing of Bioinsecticides; Search and Development of New Bioinsecticides

Section - IIUnit - 3 Primary metabolites production 15 hours

1. Amino Acids: Introduction, Microbial strain employed in aminoacid production, process control in amino acid fermentation, Production of Glutamic Acid by Wild Type Bacteria, Production of Amino Acids by Mutants, Improvements in the Production of Amino Acids Using Metabolically Engineered Organisms; Vitamin: Vitamin B12, Riboflavin, Carotenodis

2. Production of Organic Acids: Citric, acetic lactic, Gluconic and Itaconic acid 3. Production of Microbial enzyme: Introduction, Development of new enzymes, Fermentation process,

Recovery and finishing, Regulations and specification, Survey of enzyme and application 4. Production of Fermented Foods: Introduction; Fermented Food from Wheat: Bread; Fermented Foods Made

from Milk; Fermented Foods from Corn; Fermented Vegetables; Fermentations for the Production of the Stimulant Beverages: Tea, Coffee, and Cocoa; Fermented Foods Derived from Legumes and Oil Seeds; Production of Beer, Wines and Spirits

Unit - 4 Production of Secondary metabolites and other 15 hours

1. Microbial Polysaccharides and Polyesters : Polysaccharides, Xanthan Gum, Polyesters 2. Production of Ergot Alkaloids: Nature of Ergot Alkaloids, Uses of Ergot Alkaloids and their Derivates,

Production of Ergot Alkaloids, Physiology of Alkaloid Production 3. Microbial Production of Nucleocide and Nucelotides: Introduction, Methods for production 4. Single Cell Protein (SCP): Substrates for Single Cell Protein Production; Microorganisms Used in SCP

Production; Use of Autotrophic Microorganisms in SCP Production; Safety of Single Cell Protein; Nutritional Value of Single Cell Protein; Yeast Production: Production of Baker‟s Yeast; Food Yeasts; Feed Yeasts; Alcohol Yeasts; Alcohol Yeasts

List of Experiments

1. Production of Amino acid (Glutamic acid)

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M.Sc. Microbiology CBCS Curriculum 2011

2. Production of Vitamins (Vitamin B2 /Vitamin B12)3. Production of Solvents (Ethanol, Acetone/ Butanol)4. Production of Organic acid (Citric acid/ Gluconic acid) 5. Production of extra-cellular polysaccharide 6. Production of Antibiotics (Penicillin/ Tetracycline/ Chlormphanicol/ Streptomycin)7. Effectiveness of antimicrobial preservatives8. Microbial limit test 9. Physicochemical test of extracts: Preparation of extract, appearance, light absorption, pH, nonvolatile matter,

residues on ignition, heavy metal, buffering capacity, oxidizable substances10. Lal test for bacterial endotoxins

List of Reference Books

1. Mansi, Fermentation microbiology and Biotechnology, Taylor and Francis2. Waites, Industrial Microbiology: An Introduction, Blackwell publication 3. Michal, Bioprocess Engineering Basic Concept, Prentica Hall of India4. Biotol series, Operational Models for Bioreactor, 5. Biotol series, Product recovery in Bioprocess technology, 6. Whittaker, Principles of fermentation technology.7. Crueger, A text book of Industrial microbiology.8. Okafor, Modern Industrial Microbiology and biotechnology 9. Najafpour, Biochemical Engineering And Biotechnology10. Alexander, Microbial Biotechnology11. Volkmar, Microbial Fundamentals of Biotechnology12. Sikyta, Techniques in Applied Microbiology 13. Laskin, Applied Microbiology, Volume 5614. Laskin, Advances in Applied Microbiology Volume 59 15. Laskin, Advances in Applied Microbiology Volume 6216. Walker, Microbial Processes and Products17. Demain, Manual of Industrial and Biotechnology18. Peppler, Microbial technology: fermentation technology19. Waites, Industrial Microbiology: An Introduction20. Michal, Bioprocess Engineering Basic Concept21. Rehm, A multivolume Comprehensive Treaties: Biotechnology

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M.Sc. Microbiology CBCS Curriculum 2011

MB - 303 Omics, Inter Phase Microbiology and Recent Advances in Microbiology4 Credits

Section - I

Unit 1 Proteomics: Microbiology 15 hours1. Holistic Biology of Microorganisms: Genomics, Transcriptomics and Proteomics; Understanding genes,

genomes, “otheromes”; Introduction and basic concept of systems biology2. Tools and technique for proteomics 3. Exploring and Exploiting Bacterial Proteomes; Strategies for Measuring Dynamics: The Temporal

Component of Proteomics; Quest for Complete Proteome Coverage4. Proteomics of Corynebacterium glutamicum: Essential Industrial Bacterium; Analyzing Bacterial

Pathogenesis at Level of Proteome; Structural Proteomics and Computational Analysis of a Deadly Pathogen: Combating Mycobacterium tuberculosis from Multiple Fronts

Unit 2 Genomics: Microbiology 15 hours1. Tools and technique for Genomics2. Bacterial Genomes for the Masses3. Comparative Genomics for Microorganisms; Microbial Genome Sequencing and Annotation4. Pharmacogenomics : Overview, concept and application of Individualized Therapy; RNA Interference:

Targeted Medicine Section - II

Unit 3 Metagenomics 15 hours1. Metagenomics: What and Why for metagenomics2. Metagenomics: a new light on biology 3. Metagenomics: From Genomics to Metagenomics4. Designing a successful metagenomics project

Unit 4 Recent Advances in Microbiology 15 hours1. Overview of clinical laboratory diagnosis (Hematology, Cardiac, Renal, Liver testing’s) ; Culturing of

pathogens; Immunological Diagnostic Procedure; Monoclonal Antibodies; DNA diagnosis systems; Molecular Diagnosis of genetic disease.

2. Overview and Current status of Anti HIV, Anti Malaria, Anti Tuberculosis and Anti Cancer treatment; Multidrug resistance : Introduction, development, detection and treatment

3. Discovering New Pathogens; New disease: SARS, bird flu , swine flu etc.; Pharmacogenomics : Overview, concept and application of Individualized Therapy; RNA Interference: Targeted Medicine; Introduction to synthetic biology; Overview of Artificial Cells

4. Study of Selected recent review/ research paper in field of Microbiology ( Minimum four)

List of Experiments

1. DNA extraction from Soil2. DNA extraction from water

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M.Sc. Microbiology CBCS Curriculum 2011

3. Library creation from metagenome4. Isolation of m RNA5. RT PCR6. Immunological testing : Widal test; VDRL test; Enzyme Linked Immuno Sorbent assay (ELISA)7. Hematology : RBC Count; Total WBC Count; Differential WBC Count; E.S.R. determination; Hb estimation; 8. Bleeding time and clotting time9. Blood Grouping: Slide technique; Tube technique; Reverse and forward grouping/ Cross matching: Major and Minor/Coombs test: Direct coomb’s; Indirect coomb’s10. Isolation and identification of Pathogens11. Biochemistry : Cardiac Profile testing; Live Profile testing; Renal Profile testing 12. Study of genome database and tools13. Study of Metagenomics database and tools14. Study of proteomics database and tools

List of Reference Books 1. Woodford, Genomics, Proteomics and clinical bacteriology2. Andreas, Computing for Comparative Microbial Genomics3. Humphery-Smith, Microbial Proteomics4. Rehm, Protein Biochemistry and Proteomics5. Daniel, Introduction to Proteomics6. Heinrich, Industrial Pharmaceutical Biotechnology7. Richmond, Bio safety in Microbiological and Biomedical Laboratories 8. Rick, Drugs: From Discovery to Approval 9. Gad, Handbook of Pharmaceutical Biotechnology10. Walsh, Biopharmaceuticals Biochemistry and Biotechnology11. Hugo, Pharmaceutical Microbiology, Blackwell scientific Publications12. Glick, Molecular Biotechnology

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M.Sc. Microbiology CBCS Curriculum 2011

MBEL 18 Any paper of students’ choice to develop technical skill 02Semester – IV

MB 401 Project Work 18

Semester – IV Project Work

Students have to take up a small research project under the supervision of a teacher from the department or may carry the work in an industry / NGO / private laboratory with required facility/other university or institution. If a student is doing project out side the university then the chemicals to carry out experiment shall be replaced to the institute where he/she carry out the experimental work. All other arrangements are to be made by the student. Student has to submit the dissertation before the last date for the submission as declared by the university.

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL- 01 Advanced BioinformaticsUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL- 02 Microbial Genomics and ProteomicsUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL- 03 Nanotechnology and Systems BiologyUnit Topic Time Marks Credit

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1 Concepts of system biology2 Application of system biology3 Tools and resources of systems biology4 Overview of major system biology projects

List of reference books

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 04 Environmentally Sound Technologies (EST)Unit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 05 Entrepreneurship and Management for Microbiology based Industry Unit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 06 Microbiology of Extreme EnvironmentUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 07 Quality Control, Assurance and Total Quality ManagementUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 08 Microbial Physiology and BiochemistryUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 09 Medical Microbiology & ImmunologyUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 10 Agriculture MicrobiologyUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 11 Food and Dairy MicrobiologyUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 12 Soil and Water Microbiology Unit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 13 Enzyme TechnologyUnit Topic Time Marks Credit

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M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 14 Drug Discovery and Clinical ResearchUnit Topic Time Marks Credit

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Hem. North Gujarat University, Patan Page 40

Page 41: M.Sc. Microbiology CBCS Curriculum 2010 - H.N.G.U, … · Web viewM.Sc. Microbiology CBCS Based Curriculum (With effect from June 2011) Paper No Title Credit Semester – I MB-101

M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 15 Microbial Production of Recombinant Molecules Unit Topic Time Marks Credit

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Hem. North Gujarat University, Patan Page 41

Page 42: M.Sc. Microbiology CBCS Curriculum 2010 - H.N.G.U, … · Web viewM.Sc. Microbiology CBCS Based Curriculum (With effect from June 2011) Paper No Title Credit Semester – I MB-101

M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 16 Microbial EcologyUnit Topic Time Marks Credit

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Hem. North Gujarat University, Patan Page 42

Page 43: M.Sc. Microbiology CBCS Curriculum 2010 - H.N.G.U, … · Web viewM.Sc. Microbiology CBCS Based Curriculum (With effect from June 2011) Paper No Title Credit Semester – I MB-101

M.Sc. Microbiology CBCS Curriculum 2011

MB EL - 17 Contemporary MicrobiologyUnit Topic Time Marks Credit

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Hem. North Gujarat University, Patan Page 43

Page 44: M.Sc. Microbiology CBCS Curriculum 2010 - H.N.G.U, … · Web viewM.Sc. Microbiology CBCS Based Curriculum (With effect from June 2011) Paper No Title Credit Semester – I MB-101

M.Sc. Microbiology CBCS Curriculum 2011

MB EL – 18 Soft Skill (Students’ Choice)Unit Topic Time Marks Credit

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Hem. North Gujarat University, Patan Page 44