Immunology BBasic Lab Safetyasic Lab Safety WHMISumanitoba.ca/faculties/medicine/units/immunology/media/safety... · coordinator or Immunology Department Administrative staff. It

ImmunologyImmunologyImmunologyImmunologyBBasic Lab Safetyasic Lab Safety

&&WHMISWHMISWHMISWHMIS

Sam Kung & Christine MesaSafety Coordinator

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Safety CoordinatorDepartment of Immunology

W th i i Worth reviewing every yearSafety is important for everyone

S f l t kSafe place to work

Need to keep our Biosafety Permit in order to do our workp y

Need to obtain a Biosafety Certificate for each new research grant we received new research grant we received

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To apply for a Biosafety Certificate:

In addition to the funded project summary, short description of the proposed work, ethical approvals, signed form.

Active and updated biosafety permit (inventory, location, users, completed training courses, lab-specific SOP).)

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THANK YOUTHANK YOUChristine MesaKennedy MakondoBill Stefura

Steve Cole, EHSO

LMC

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All of you

OUTLINE OF THIS PRESENTATION

ResponsibilityChecklistChecklistWorking Alone PolicySpillsFire Safety

L b ifi S f t P ( h i l bi l i lLab specific Safety Program (chemical, biological, CLIP)

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Responsibilities for SafetyResponsibilities for Safety

U of M Health and Safety Policy

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U of M Health and Safety Policies- Laboratory Safety Training Standard

77 Responsibilities for SafetyResponsibilities for Safety

Principal Investigator / Supervisors:

provide workers with:

- a safe work environment- adequate safety equipment- safety training

Responsibilities for SafetyResponsibilities for SafetyEmployees and Students:

Responsibilities for SafetyResponsibilities for Safetyp y

- follow Departmental and Principal Investigator’s established safety protocols

- use reasonable care to protect your safety and the safety of otherssafety of others

-bring safety concerns to the attention of the principal investigator or the designated safety individual.

HAVE THE RIGHT TO REFUSE TO DO WORK THAT YOU

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HAVE THE RIGHT TO REFUSE TO DO WORK THAT YOU CONSIDER TO BE UNSAFE

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Accident/Incident ReportingAccident/Incident Reporting

Legislation requires that all accidents, incidents and near misses shall be investigated and a


and near misses shall be investigated and a report completed.

Incidents include the following:personal injury (including needle sticks)occupational illnessoccupational illnessfire/explosionproperty and equipment damage

ienvironmental damagenear miss incidents (those incidents that could have resulted in any of the above l )losses)


Staff and Students:

Report the incident to your supervisor immediately: verbally or using the Employee’s Green Card-Notice of Injury Form available on EHSO web-site Notice of Injury Form available on EHSO web site and also in the folders near main entrance of the lab.

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11 Accident/Incident ReportingAccident/Incident Reporting

Wh i id t t

Procedures- Serious Accidents and Injuries

When a serious accident occurs at a workplace, the employer (UM) must notify Manitoba Labour-Workplace Safety and Health Di i i i kl iblDivision as quickly as possible.

During regular hours: EHSO at 474-6633. If after hours: U of M Security at 555 or HSC at 55If after hours: U of M Security at 555 or HSC at 55

Serious accidents include:a death or serious injuryuncontrolled spill of a hazardous materialexplosion fire or floodingexplosion, fire or flooding

Chemical (WHMIS) and Biological Safety Checklist for Staff, Students and Visitors

Name: Position: Lab: Start date: For any new person working in the department please fill out this form and give copy to WHMISFor any new person working in the department, please fill out this form and give copy to WHMIS coordinator or Immunology Department Administrative staff. It is the responsibility of the PI to ensure that any staff, student, or visitor/user coming to use equipment in the lab completes/has completed the U of M online Biological Safety training and quiz, and the online WHMIS training and quiz.q , g q The following documents replace the old Immunology Biosafety questionnaire and can be found at this website: http://umanitoba.ca/admin/human_resources/ehso/bio_safety/biosafety.html L b t S f t Ch kli t f N L b P l ( t b k t b WHMIS Laboratory Safety Checklist for New Lab Personnel (copy to be kept by WHMIS

coordinator OR Department Administrative office) EHSO Generic Biosafety training Powerpoint Biosafety Quiz

WHMIS Program g http://umanitoba.ca/admin/human_resources/ehso/chemical_safety/WHMISProgram.html WHMIS Online Training Presentation WHMIS Test

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There will be a yearly departmental WHMIS presentation, which is mandatory for anyone working in the department to attend.

Chemical (WHMIS) and Biological Safety Checklist for Staff, Students and Visitors (cont’d) ( )

PIs are also responsible for ensuring new staff/students obtain vaccines if their work exposes them to vaccine preventable diseases: htt // it b / d i /h / h / h lth /I i ti ht lhttp://umanitoba.ca/admin/human_resources/ehso/occ_health_comp/Immunization.html Fill out and submit Risk Assessment worksheet

If you work with Human blood and body fluids or Animals, please post the Post Exposure protocol in your lab areas, so staff/students know how to deal with accidental exposure from cuts orin your lab areas, so staff/students know how to deal with accidental exposure from cuts or needlestick injuries. Each lab also needs to have a working alone policy http://www.umanitoba.ca/admin/governance/governing_documents/staff/839.html New Staff/Student/Visitor is aware of working alone policy

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Department of Immunology Safety Self-Assessment online formy

New trainees should complete the questionnaire and discuss it with the supervisor

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Departmental Working Alone PolicyDepartmental Working Alone PolicyWorkplace Safety and Health Act recognizes that certain workplaces require staff to work alone and therefore

Departmental Working Alone PolicyDepartmental Working Alone Policy

workplaces require staff to work alone and therefore requires that a plan is in place that is agreeable to both the employer and employee. The plan should be based on a realistic risk assessment of the hazards under the

i t d i l d itt circumstances and include a written emergency response plan

Review your Department’s “Working Alone Policy” – The y p g yUzonna Policy that includes:

Mandatory buddy system where staff and students must work in pairs.

Supervisor notification before starting work after hours.

Restrictions to certain types of work.

University of Manitoba: University Governance - Working Alone: Procedures

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General Lab Safety: Spills - General GuidelinesGuidelines

Additional Departmental Spill Kit SuppliesAdditional Departmental Spill Kit SuppliesI dditi t b i l b ill kit D t t In addition to basic lab spill kits, Departments

should also provide the following:

1) Emergency contact lists.2) Commercial mop and pail.3) Full size “washable” broom and dustpan 3) Full size washable broom and dustpan. 4) Respirator with multi-purpose P100 cartridges

suitable for acids, ammonia, amines, chlorineorganic vapours as well as fine particulatesorganic vapours as well as fine particulates,and dusts.

5) Additional “emergency” stash of disposable supplieslike universal pads caution tape gloves disposable like universal pads, caution tape, gloves, disposable bags and autoclave bags.

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Fire SafetyFire SafetyGet to know the alarm bell scenarios in your

building.

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g

When you hear a fire alarm you should turn off all flames and gas sources and prepare to exit the b ildi i th t i building via the stairway.

If you need assistance proceed to nearest stairway and await the FIRE WARDENand await the FIRE WARDEN

Know the area where to assemble

Karen and Susan are our Fire MarshallsKaren and Susan are our Fire Marshalls

Contacts for departmental fire information / training and the fire ardens are posted at o r training and the fire wardens are posted at your Departmental Office.

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L b S ifi S f t PL b S ifi S f t PLab Specific Safety ProgramsLab Specific Safety Programs

Chemical (WHMIS)

Biological

Combined Lab Inspection Program (CLIP)Combined Lab Inspection Program (CLIP)

Radiation

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Chemical (WHMIS)

WWorkplaceorkplaceWWorkplaceorkplaceHHazardousazardousHHazardousazardousMMaterialsaterialsMMaterialsaterialsIInformationnformationIInformationnformationSSystemystemSSystemystem

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WHMIS: GoalWHMIS: Goal

ll f

WHMIS: GoalWHMIS: Goal

To reduce injury and illness from exposure to hazardous materials in the workplacew r p

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WHMIS: Three Key ElementsWHMIS: Three Key Elements

1. Worker Education and Training

2. Labels

3. Material Safety Data Sheets (MSDS)

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How to properly store chemicals…How to properly store chemicals…

Help identify safe storage of chemicals (ie. Acetic Acid is a flammable and should be in flammable cabinet and not just under fume hood)

Help identify incompatible chemicals (ie. Hydrogen p y p y gPeroxide and Ethanol should not be stored together—an oxidizer and a flammable)

And how to properly dispose of And how to properly dispose of chemicalschemicalschemicals…chemicals…

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WHMIS at the U of MWHMIS at the U of M

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General Chemical OrganizationGeneral Chemical Organization

Flammables Store in flammable storage cabinets

Bases Store separately p y

Oxidizers Store separately

Acids Store in corrosive resistant acid cabinet

EXCEPT: chromic nitric and perchloric acidsEXCEPT: chromic, nitric, and perchloric acids which should be stored separately

Glacial acetic acid should be stored as a flammableflammable

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WHMIS Controlled Products: WHMIS Controlled Products: Hazard Classes & SymbolsHazard Classes & Symbols

Class A : Compressed GasesClass A : Compressed Gases

Hazard Classes & SymbolsHazard Classes & Symbols

Risks:

Class A : Compressed GasesClass A : Compressed Gases

Physical hazard (120kg)

Explosive hazard(if heated dropped or impacted)(if heated, dropped or impacted)

Content hazard(could be flammable,poisonous)Examples: ( f ,p )p

CO2 tanksN2 tanks

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N2 tanks02 tanks


St d T t tiClass A : Compressed GasesClass A : Compressed GasesHazard Classes & SymbolsHazard Classes & Symbols

Storage and Transportation:Secure cylinder

Store in cool ventilated area

Transport using specialized cart

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Class A : Liquid NClass A : Liquid N22 & Dry Ice& Dry IceRisks

Frostbite

Samples may explode

Asphyxiation

Handling and UseAvoid skin contactWear insulated gloves and eye protectionStore in a well ventilated roomTransport securely to prevent accidental spillage Store Liquid N2 in a vented dewarDO NOT STORE DRY ICE IN WALK‐IN Freezer!

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WHMIS Controlled Products: WHMIS Controlled Products: Hazard Classes & SymbolsHazard Classes & SymbolsClass B : Flammable & CombustibleClass B : Flammable & Combustible


Six Subdivisions and examples:1. Flammable Gases - hydrogen, methane

2 Flammable liquids [flash pt <37 8C]-2. Flammable liquids [flash pt <37.8C]-gasoline, acetone, ether, ethanol

3. Combustible Liquids [flash pt >37.8C] - kerosene, lvarsol

4. Flammable Solids - magnesium metal,aluminum dustaluminum dust

5. Flammable Aerosols - aerosol containers

6 R ti Fl bl M t i l h h di 6. Reactive Flammable Materials - phosphorus, sodium metal

WHMIS Controlled Products: WHMIS Controlled Products: Hazard Classes & SymbolsHazard Classes & SymbolsHazard Classes & SymbolsHazard Classes & Symbols

Class B : Flammable & CombustibleClass B : Flammable & Combustible

Risks:Fi h d ill b if i i d

Class B : Flammable & CombustibleClass B : Flammable & Combustible

Fire hazard – will burn if ignited

Could ignite spontaneously(under adverse conditions)(under adverse conditions)

Could ignite upon mixing with wateror other chemicals

Many are poisonous

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Class B : Flammable & CombustibleClass B : Flammable & CombustibleStore in flammable storage cabinetsClass B : Flammable & CombustibleClass B : Flammable & Combustible

Transport securely to prevent accidental spillage

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Class C : Oxidizing ProductsClass C : Oxidizing ProductsRisks:

Class C : Oxidizing ProductsClass C : Oxidizing Products

Increase fire and explosion hazard of flammable and combustibles

Potentially explosive when mixed with Potentially explosive when mixed with flammables or organics

Most are corrosive and poisonousp

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Class C : Oxidizing ProductsClass C : Oxidizing Products


Examples:Any oxygen source including:

OO2 gasperoxides (If refrigeration

required, use spark-free fridge)q , p g )nitrates / nitriteschlorates / chloriteshypochlorites (bleach)hypochlorites (bleach)perchloratesdichromates

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permanganatespersulfates

When storing or handling ALWAYSWhen storing or handling, ALWAYSseparate class C from class B

-At present we share the the yellow flammable storage

k / k cabinets; take note/care to keep incompatible chemicals separate!

WHMIS Controlled Products: WHMIS Controlled Products: H d Cl & S b lH d Cl & S b lHazard Classes & SymbolsHazard Classes & Symbols

Class D : Toxic ProductsClass D : Toxic ProductsClass D : Toxic ProductsClass D : Toxic Products

Acute (Immediate)

Chronic (Delayed)

Biohazard36

Biohazard


Class D : Toxic Class D : Toxic -- AcuteAcute


Class D : Toxic Class D : Toxic -- AcuteAcute

Examples:All h l (B Cl F I)All halogens (Br, Cl, F, I)Cyanides & nitriles (HCN)Heavy metals like arsenic,cadmiumyHeavy metal salts like nickel

acetateHydrogen Sulfide Nitrogen Hydrogen Sulfide, Nitrogen

dioxide

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Class D : Toxic Class D : Toxic -- ChronicChronic


Class D : Toxic Class D : Toxic ChronicChronicExamples:Heavy metals like mercury & leady y

Solvents like formaldehyde, benzene, carbon tetrachloride

Reagents like acrylamide, ethidiumbromide

Numerous carcinogens, mutagens and teratogens

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Class D : Toxic Class D : Toxic -- BiohazardBiohazard


Includes all Risk Group 2-4 organisms that can cause disease in humans and animals

Examples:Human blood, tissue and body fluidsAnimal blood, tissue and body fluidsTissue culture cell linesTissue culture cell linesExperimental bacterial, viral and yeast cultures

39Health Canada’s

“Laboratory Biosafety Guidelines”


Class E : Corrosive ProductsClass E : Corrosive ProductsRisks:

Will b h ti i l di

Class E : Corrosive ProductsClass E : Corrosive Products

Will burn human tissues including skin, eyes, mouth, throat & lungs

Will corrode many lab related Will corrode many lab related materials particularly metals

Fumes may be environmentally damaging

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Class E : Corrosive ProductsClass E : Corrosive Products


Class E : Corrosive ProductsClass E : Corrosive ProductsExamples:

Most oxidizing agentsMost oxidizing agents

Strong acids & bases

Gases like hydrogen fluoride, hydrogen chloride, chlorine,y g , ,bromine, sulfur dioxide

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Class F : Dangerously ReactiveClass F : Dangerously Reactive

Risks:

Class F : Dangerously ReactiveClass F : Dangerously Reactive

Can react violently under certainconditions including:

contact with water or other chemicalscontact with water or other chemicalsif heated or shakenif allowed to polymerizep yCan release poisonous vapors when mixed with other chemicals

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Chemical StorageChemical StoragePotentially Explosive ChemicalsPotentially Explosive ChemicalsPotentially Explosive ChemicalsPotentially Explosive ChemicalsPicric Acid and Nitro Compounds

Dry picric acid may explode if subjected to heat, shock, or friction y p y p j(opening the lid)

Picric acid must be stored under wet.Some nitro compounds may have similar requirementsSome nitro compounds may have similar requirements

Peroxide Forming CompoundsExample ethers, dioxanes, sodium amideExample ethers, dioxanes, sodium amidePeroxide formation may be initiated by light or airPeroxides are prone to explosive decomposition when subjected to

heat shock or friction (opening the lid)heat, shock, or friction (opening the lid)

Evaluate the conditions of these chemicals regularly

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WHMIS: Three Key WHMIS: Three Key ElementsElementsElementsElements

Education

Labels

Material Safety Data Sheets Material Safety Data Sheets

(MSDS)

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WHMIS Labels

Workplace Labels:Labels:

1. Product Name

2 MSDS 2. MSDS Reference

3. Safe Handling Instructions

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WHMIS Labels

Labels Must be in English on: aliquots from an original containerproduct where original label is lost or illegiblep g gproducts produced and used at the workplace

MethanolMethanol

x

xMethanol

Flammable poisonous harmful vaporx

xx

Flammable, poisonous, harmful vaporKeep away from heat, sparks, flamesAvoid contact with eyes and skin

l l l l l E Workplace labels available via UofM EHSO office – or printed from the website. Stocks are available in the folders.

WHMIS Th K El tWHMIS Th K El tWHMIS: Three Key ElementsWHMIS: Three Key Elements

Education

Labels

Material Safety Data Sheets Material Safety Data Sheets

(MSDS)

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WHMIS MSDS: Material Safety Data SheetDetailed information about the controlled product.

Must be provided by the supplier.

It is against the law to use a controlled products in the workplace without the presence of an MSDS.

Must be no more the three years old from the dateproduced or revised.

If you have synthesized a product you must prepare a If you have synthesized a product, you must prepare a MSDS.

Risk Group 2 and higher biological agents also require an MSDS (ie Pertussis toxin Leishmania spp etc)an MSDS (ie. Pertussis toxin, Leishmania spp., etc).

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WHMIS MSDS:K h l i f MSDS Know the location of your MSDS collection – (Main Entrance beside first id ki )aid kit)

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U of M WebU of M Web Accessible Chemical Accessible Chemical WHMIS: Chemical Inventories

M nit b W rk pl c

U of M WebU of M Web--Accessible Chemical Accessible Chemical Inventory DatabaseInventory Database

Manitoba Work-place Health Hazard Regulation requires that a chemical inventory be a chemical inventory be maintained of all controlled products in the workplace.

Every PI’s Online Inventory Database

!!needs to be updated!!

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Chemical StorageCabinets under Fume Hoods labeled for different chemicals:AcidsBasesExtremely hazardousy

LMC to discuss coordinating orders of

id /b /fl bl (i acids/bases/flammables (ie. 5-4L jugs of xylene/methanol TOO MANY!)

Every PI’s Online Inventory Database needs to be updated (location, volumes)

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updated (location, volumes)

WHMIS: Chemical Waste Disposal

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Back to you Sam….

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Biological

Biological Containment StandardsBiological Containment Standards

jMajor Resources

•Laboratory Biosafety Guidelines 3rd Edition

•Containment Standards for Veterinary Facilities

•Occupational Health and Safety in the Care and Use of Research AnimalsUse of Research Animals.

Biosafety Level 2Biosafety Level 2

Ri k 1 2Risk groups 1, 2

Bi l i l A t Ri k GBiological Agent Risk Groups

Risk Group 1

Poses no special hazard and should not affect a healthy person or animal in any negative way Example: E coli gene negative way. Example: E. coli gene expression host.

Bi l i l A t Ri k GBiological Agent Risk Groups

Risk Group 2

Pathogens that can infect humans and animals but the infection is not serious, it is self resolving or treatable and should not spread resolving or treatable and should not spread. Example: Campylobacter jejuni or Salmonella spp.

Class II Biological Safety CabinetsClass II Biological Safety Cabinets

Air flow is in two directions.User and product protectionType A 70% of air is recirculatedM t i t th l bMay vent into the labMay be thimble or soft ducted exhaust to outsideexhaust to outside

Personal Protective EquipmentPersonal Protective Equipment

Protect the:

Eyesy

Face

Respiratory tractp y

Body

Hands

Feet

http://www.carbuyersnotebook.com/archives/Da-Vinci-Man.jpg

Face ProtectionFace ProtectionFace shield

Googles

http://www.fixandweld.com/images/SSP11.jpg

Protect the BodyProtect the Body

Lab coatBack fastening gownGreensPaper coveralls (bunny suit)Positive pressure Blue SuitPositive pressure Blue Suit

http://www.cihr-irsc.gc.ca/images/a.jpg

Protect the HandsProtect the Hands

Gloves

Disposable vinyl, nitrile, latex for protection from LAA’s and low exposure to chemicals

Sterile disposable for good mouse work

Heavy rubber chemical protection

Protect the Feet and LegsProtect the Feet and Legs

Shoes with closed toe and heel.

P tPants

Intent is to provide some barrier your skin and splashes or falling sharpsfalling sharps

HygieneHygiene

Wash your hands (soft soap best)Wash your hands (soft soap best)

Food/beverages not allowed in laboratory spaces, carts, storage rooms including:g

• Gum• Water bottles coffee cupsWater bottles, coffee cups• Food evidence in garbage containers

Post ExposurePost Exposure

l l h d dGeneral protocol – there is a standard EHSO version

Human blood and body fluids protocol – there is a standard EHSO versionEHSO version

A project specific post exposure protocol may be needed for work with exotic agentsg

Biosafety:yBiological Waste Handling

Common Types of Biological WasteCommon Types of Biological Waste

Solids – sample tubes, gloves gowns and other non sharpsLiquids - wet stuffqSharps – needles, used glassware pipettes and pipette tips

Carcasses and tissues

Biological Solid Waste DisposalBiological Solid Waste Disposal

Place in clear autoclave bag Mark with autoclave tape plus biohazard symbol

l f 1 h 121 C i iAutoclave for 1 hour at 121oC minimumThis is appropriate only when radioisotopes and hazardous chemicals are absentchemicals are absentEHSO can assist with risk assessments

Biological Liquid Waste DisposalBiological Liquid Waste Disposal

Package in a leak proof autoclave container (stainless steel, polypropylene or Pyrex glass).Mark with autoclave tape and biohazard symbolDon’t over fill and leave the cap looseAutoclave at 121oC for 60 minutes minimum or 60 minutes/gallon whichever is greatergRemove or deface biohazard logo afterwardsAfter decontamination you can drain dispose, dilute with water and flush down the drain with copious amounts of waterp

Biological Liquid Waste DisposalBiological Liquid Waste Disposal

For materials which are unsafe for autoclaving like radioactive materials or hazardous chemicals

Chemical decontamination is usefulBleach may work but at a higher concentrationDecontamination is not instant, there are exposure times Decontamination is not instant, there are exposure times required

Biologically Contaminated SharpsBiologically Contaminated Sharps

Collect in an approved biohazardous sharps container only

Mark with autoclave tape

Autoclave at 121oC for 1 hour minimum

Label with waste tag and dispose of through EHSO

Proper Sharps DisposalProper Sharps DisposalApproved Container No Hand Re-Capping

Don’t OverfillDon t Overfill

Overfilled Biohazardous Sharps ContainerOverfilled Biohazardous Sharps Container

General Lab Safety Waste Disposal General Lab Safety Waste Disposal Procedures

Segregate Biological from Chemical from

General GuidelinesGeneral Guidelines

Segregate Biological from Chemical fromRadioactive

waste at source.NO i k di l f h i lNO sink disposal of chemicals(with limited exceptions as per U of M waste disposal

chart)Use clear autoclave bags for biological hazardous

waste

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Biosafety:Biosafety:Disinfection and Decontamination

Disinfection vs SterilizationDisinfection vs. Sterilization

What’s the difference?

Disinfection vs Sterilization Disinfection vs. Sterilization Disinfection

Reduction in the number of microorganisms in or on an inanimate matrix to an acceptable level

St ili tiSterilizationComplete destruction of all living or viable organisms with a probability of one in one million that any viable agents remain. p y y g(p=1/1x106)

Common Types of DisinfectantsCommon Types of DisinfectantsBleach (Halogen)

Alcohol

Clidox (Chlorine dioxide)

Virkon (Oxidizers)

Iodine (Iodophores)

BleachBleach

Active ingredient: Hypochlorous acid (HOCl)Best pH 7.520 30 20-30 minute exposure timeGood broad spectrum activity

Mix and use FRESH!!!

How to use BleachHow to use Bleach

1:5 effective for biohazardous spill clean up. 1:5 effective for biohazardous spill clean up. 1:10 effective surface decontaminant (if surface can stand it).1 20 l d f (Cl l b l)1:20 general disinfectant (Clorox label)1:100 commonly used for routine wipe down of surfaces.

Notes:At pH below 7 activity is quickly compromised.Breaks down in the presence of organic materialBreaks down in light and with some metals so avoid in Breaks down in light and with some metals so avoid in storage

Bleach DANGER!Bleach – DANGER!

Addition of acid to bleach releases chlorine gas!

How to use AlcoholHow to use Alcohol

Active form: 70% vol/vol ethanol or isopropanolBest when wiped on and left wetGreat skin disinfectant24 hour contact time for surgical instruments

Note:Evaporation causes dilution and decreased effectivenessEvaporation causes dilution and decreased effectiveness

Alcohol DANGERAlcohol -DANGER

Flammable! Avoid spark and flame especially in Biosafety carbinet!

Autoclave SterilizationAutoclave Sterilization

Uses heated moist air (steam) Most efficient transfer of heat Will break H-bonds, Causes coagulation and denaturation of proteins and nucleic acidsacids

Autoclave SafetyAutoclave Safety

DangersDangers

Heat

SteamSteam

Pressure

Autoclave SafetyAutoclave SafetySafer practicesp

Do not autoclave chemicals that are flammable, explosive or that decompose with heating! Refer to MSDS!MSDS!Do not autoclave materials that will melt in the autoclave without secondary containment to contain the liquid F ll d l d lFollow departmental procedure in using autoclave.Allow the autoclave to cool (10 min)Open the autoclave door just a crack at first to vent steam and Ope t e autoc ave oo just a c ac at st to ve t stea a heatDo not lean into the autoclave, pull the load out to you!!!M h l d Move hot loads on a cart

Autoclave SafetyAutoclave SafetyProtective equipment

Lab coatThermal insulated gloves

f lSafety glassesIf the load has large volumes of liquids a rubber splash apron should be usedapron should be used

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Combined Lab Inspection Program

(CLIP)

Kudos to everyone in the Department

Congratulations and THANK YOU for the Congratulations and THANK YOU for the remarkable efforts to keep the Department of

Immunology a Safe Working Place!!!gy g

CLIP citations this year

Secure the CO2 Tanks.

WHIMS labels

Worn-out electrical wirings

N d I tNeeds Improvements:

Body protection

Prompt Removal of autoclaved bags from the autoclave after use.

Prompt removal of the chemical or sharp wastes do not accumulatewastes – do not accumulate.

THANK YOU

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Immunology BBasic Lab Safetyasic Lab Safety WHMISumanitoba.ca/faculties/medicine/units/immunology/media/safety... · coordinator or Immunology Department Administrative staff. It

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