Effects of liquorice on beta- amyloid aggregation and toxicity in Caenorhabditis elegans

Folie 1

Effects of liquorice on beta-amyloid aggregation and toxicity in Caenorhabditis elegansPille Link, Prof. Dr. M. WinkUniversity of HeidelbergInstitute for Pharmacy and Molecular BiotechnologyConflict of interest: nothing to declare

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Glycyrrhiza glabraUsed to treat ulcersgastritiscoughlonging for sweets- 3 -

http://www.alz.org/braintour/images/plaques_tangles.jpg- 4 -Alzheimers diseaseoxidative stressinflammationneuronal cell deathloss of memoryA plaquesantioxidantanti inflammatoryneuroprotectivememory-enhancingG. glabra?neurofibrillary tanglesamyloid- plaquesAsl MN & Hosseinzadeh H (2008). Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds. Phytotherapy Research 22: 709-724.Caenorhabditis elegans: a model for Alzheimers diseaseCL2006A42 expression in muscle cells number of plaques

CL4176temperature inducible A42 expression in muscle cellsprogressive paralysis A42 toxicity- 5 -

Link CD (2006). C. elegans models of age-associated neurodegenerative diseases: lessons from transgenic worm models of Alzheimer's disease. Exp Gerontol 41: 1007-1013.

Does G. glabra have an effect on A aggregation? - 6 -CL2006: thioflavin S staining- 7 -

G. glabraG. glabraGlycyrrhiza glabra- 8 -* p < 0.01glycyrrhizic acid (GA)

glycyrrhetinic acid (GRA)

isoliquiritigenin (ILG)concentration: 100 g/ml

Can G. glabra reduce A-induced toxicity?- 9 -

Paralysis assay in CL4176temperature inducible A expressionparalysis due to A toxicity- 10 -Activity against A-toxicity- 11 -Conclusions & outlookextract & pure compounds from G. glabra can:decrease the number of A plaquescounteract A toxicity

most active compound: isoliquiritigenin

further work:test more compuondsclarify the mode of action- 12 -

Thank you for your attention!- 13 -

IPMBUniversity of Heidelberg