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DEVELOPMENT OF ATMOSPHERIC PLASMA JET FOR PORK SKIN TREATMENT BY KAMONCHANOK DEEMEK A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF ENGINEERING (ENGINEERING TECHNOLOGY) SIRINDHORN INTERNATIONAL INSTITUTE OF TECHNOLOGY THAMMASAT UNIVERSITY ACADEMIC YEAR 2016
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Page 1: DEVELOPMENT OF ATMOSPHERIC PLASMA JET FOR PORK SKIN …

DEVELOPMENT OF ATMOSPHERIC PLASMA JET

FOR PORK SKIN TREATMENT

BY

KAMONCHANOK DEEMEK

A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF

THE REQUIREMENTS FOR THE DEGREE OF MASTER OF

ENGINEERING (ENGINEERING TECHNOLOGY)

SIRINDHORN INTERNATIONAL INSTITUTE OF TECHNOLOGY

THAMMASAT UNIVERSITY

ACADEMIC YEAR 2016

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DEVELOPMENT OF ATMOSPHERIC PLASMA JET

FOR PORK SKIN TREATMENT

BY

KAMONCHANOK DEEMEK

A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF

THE REQUIREMENTS FOR THE DEGREE OF MASTER OF

ENGINEERING (ENGINEERING TECHNOLOGY)

SIRINDHORN INTERNATIONAL INSTITUTE OF TECHNOLOGY

THAMMASAT UNIVERSITY

ACADEMIC YEAR 2016

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Abstract

DEVELOPMENT OF ATMOSPHERIC PLASMA JET FOR PORK SKIN

TREATMENT

by

KAMONCHANOK DEEMEK

Bachelor of Science, King mongkut's institute of technology ladkrabang, 2014

Master of Engineering (Engineering Technology), SIIT, Thammasat University, 2016

This study aims to develop the plasma jet for animal skin treatment. There are three

designs of atmospheric plasma jet system consider. The impacts of control parameters,

including applied voltage, applied frequency, and flow rate of argon gas is also

investigated in order to optimize the performance of the plasma jet. The power

discharge, plasma plume length and the optical emission spectrum are used in the

performance optimization. The results show that the similar trends are observed from

those 3 designs considered, in which the discharge power increases with the increase of

supplied voltage and frequency with 95% confidential based on ANOVA analysis.

However, it is found that the discharge power does not depend on the Argon flow rate.

It is also found that plasma plume length has the correlation with applied voltage, the

frequency and flow rate. In addition, the OES spectrum shows that the trend line of

spectrum will increase if the applied voltage or the frequency increase. Furthermore the

trend line of spectrum also show that the flow rate 2 liter/minute yield the best

performance.

Keywords: Atmospheric pressure plasma , Plasma jet, Power discharge

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Acknowledgements

At first I would like to express my sincere thanks to my thesis advisor,

deeply grateful to Associate Professor Dr. Thawatchai Onjun for his valuable helping

and supporting. I am most grateful for his bearing teaching and advising me throughout

the course of this research. Without all the support that I have always received from

him I would not have achieved this graduation and this thesis would not have been

completed.

Besides my advisor, I would like to acknowledge and pay my sincere

thanks my committee members, Associate Professor Dr. Paiboon Sriarunothai,

Associate Professor Dr. Shinsuke Mori and Dr. Udom Sae-Ueng for serving as my

committee member and alway helping provide an useful advice.

My sincere thanks also goes to Dr. Nopporn Poolyarat for always helping,

supporting by giving an area for make this experimental. Also Dr. Puwanan chumthong

who always guiding and helping me until the end of this period.

I would also like to acknowledge the Plasma Fusion Research Unit group

members for all helping, encouraging and supporting me.

I gratefully acknowledge the scholarship received from TAIST-Tokyo tech.

I greatly appreciate all teachers for their patient instruction and my classmates for their

endless friendship and encouragement. I really appreciate all the kind support from the

program staff during my entire program of study.

Last but not the least, for my beloved family and my lovely friends. I am

very appreciate for all their supporting, loving and believing in myself. Also my friends

who were encourage me until the end of this research period.

Ms. Kamonchanok Deemek

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Table of Contents

Chapter Title Page

Signature Page i

Acknowledgements ii

Abstract iii

Table of Contents iv

List of Figures (if any) v

List of Tables (if any) vi

1 Introduction 1

1.1 Introduction 1

1.2 Statement of problem 3

1.3 Scope of study 4

1.4 Significance of the study 4

2 Literatures Review 5

2.1 Basic and Fundamentals of plasma 5

2.1.1 Plasma criteria 7

2.2 Generation of plasma 8

2.2.1 Ionization process 9

2.3 Plasma categories 10

2.3.1 Thermal Equilibrium Plasma or Thermal Plasma 12

2.3.2 Non Thermal Equilibrium Plasma or Non Thermal plasma 12

2.4 Atmospheric pressure plasma jet 14

2.5 Free radicals 15

2.6 Design of atmospheric plasma jet 17

2.7 Current research of atmospheric plasma jet 19

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2.8 Brief information on the animal skin 22

2.8.1 Information about skin 22

2.8.1.1 Porcine skin 23

2.8.1.2 Structure of porcine skin 24

2.8.1.3 Structure of the cell membrane 25

2.9 Wettability 25

3 Development of atmospheric plasma jet 27

3.1 Three designs of plasma jet and power supply system 27

3.2 Structure of atmospheric plasma source APPJ 29

3.3 Parameters to identify the performance of plasma jet 32

3.3.1 Measurement of power discharge 32

3.3.2 Measurement of plasma plume length 34

3.3.3 Optical Emission spectroscopy characteristic 34

4 Results and discussion 35

4.1 Discharge power of plasma jet 35

4.1.1 Discharge power of design 1 35

4.1.2 Discharge power of design 2 36

4.1.3 Discharge power of design 3 37

4.2 Plasma plume length of plasma jet 37

4.2.1 Plasma plume length of designl 1 38

4.2.2 Plasma plume length of design 2 39

4.2.3 Plasma plume length of design 3 40

4.3 Optical Emission Spectroscopy of plasma jet 41

4.4 Impact of plasma jet on pork skin 42

5 Conclusions and Recommendations 44

5.1 Conclusions 44

5.2 Recommendations 44

References 45

Appendix

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List of Tables

Tables Page

2.1 The differentiation between plasma state and gas state 6

2.2 The major characteristics between Thermal plasma and

Non- thermal plasma 11

2.3 List of the bacteria both of Gram – negative and Gram positive

with report that APPJ can effect 16

4.1 The summary results of plasma jet in three designs 41

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List of Figures

Figures Page

1.1 Types of sterilization / disinfection method 2

2.1 The state of matter 5

2.2 Gas and plasma state: In a plasma, the gas’s electron were

ionized and ripped out from nucleus. The plasm state consists

of charged particles both negative and positive ions 5

2.3 Plasma in nature 7

2.4 Plasma application for surface modification 8

2.5 The approximate of breakdown voltage for several gas

follow the Paschen’s law 10

2.6 The assortment of plasma state (in term of the temperature of electrons

Versus the density of electrons) 11

2.7 Show the effect between the temperatures and pressure on the nature

of the plasma 13

2.8 The schematic of plasma jet 14

2.9 The source of free radicals 15

2.10 Effect of treatment distances on OH radical production 17

2.11 Schematic of atmospheric plasma jet 18

2.12 Show the main structure of skin 23

2.13 Comparative histological aspect of porcine (A) and human (B) skin

(haematoxylin-eosin-saffron staining). HF: hair follicle, Mu and arrowhead:

arrector pili muscle, SwG: Sweat gland, SG: sebaceous gland, Ad:

Adipocytes (hypodermis) 24

2.14 Histologic section of pig skin from the abdomen H&E staining 10X 25

2.15 Contact angles for hydrophobic and hydrophilic surfaces 26

2.16 Different between hydrophobic (right) and hydrophilic (left) 26

3.1 Shown the schematic of plasma jet design 1 27

3.2 Shown the schematic of plasma jet design 2 28

3.3 Shown the schematic of plasma jet design 3 28

3.4 The experiment set up in the laboratory 29

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3.5 Function generator MCP SGN1638N model 30

3.6 Power Amplifier 200watt 31

3.7 Ignition coil 32

3.8 Some result of power discharge from MATLAB software calculation 33

3.9 The conical shape of plasma jet on each design 34

4.1 The plasma discharge power obtained from a jet system design 1

is shown as a function of applied frequency at different applied potential 35

4.2 The plasma discharge power obtained from a jet system design 2

is shown as a function of applied frequency at different applied potential 36

4.3 The plasma discharge power obtained from a jet system design 3

is shown as a function of applied frequency at different applied potential 37

4.4 The result of plasma plume length of design 1 shown as a function of

applied frequency whereas the applied potential was fixed at 2kV at any

flow rate range 2-5 liter/minute 38

4.5 The result of plasma plume length of design 1 shown as a function of

applied frequency whereas the applied potential was fixed at 3kV at any

flow rate range 2-5 liter/minute 38

4.6 The result of plasma plume length of design 2 shown as a function of

applied frequency whereas the applied potential was fixed at 2kV at any

flow rate range 2-5 liter/minute 39

4.7 The result of plasma plume length of design 2 shown as a function of

applied frequency whereas the applied potential was fixed at 3kV at any

flow rate range 2-5 liter/minute 39

4.8 The result of plasma plume length of design 3 shown as a function of

applied frequency whereas the applied potential was fixed at 2kV at any

flow rate range 2-5 liter/minute 40

4.9 The result of plasma plume length of design 3 shown as a function of

applied frequency whereas the applied potential was fixed at 3kV at any

flow rate range 2-5 liter/minute 40

4.10 The emission spectra of the plasmas produced with Argon gas,

top figure is shown at the applied potential 2kV. Bottom figure

is shown at the applied potential 3kV 41

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Chapter 1

Introduction

1.1 Introduction

Plasma technology has played an important role in industrials process

nowadays, especially in semiconductor and electronics industries [1-9]. In fact, plasma

is one of the four fundamental states of matter. It consists of positive and negative

charged particles. There are many types of plasma sources, such as DBD ( Dielectric

Barrier Discharge) [20-22], Plasma jet [22-24], arc and plasma torch, corona discharge

and low-pressure discharge [21, 25]. Atmospheric pressure plasma jet is one of

interesting plasma source because it can be operated in the ambient air, and does not

require a vacuum system. As a result, there is no limitation of the size of chamber [21,

25]. Plasma jet is convenient to handle or use, especially for applying on the specific

area. In addition, atmospheric pressure plasma jet has a characteristics to be able to

induce a high chemical reactivity [26-29].

Nowadays, plasma can be applied on various applications, such as

automotive[30] with mainly used to deposit hard films that provide protection from heat, wear,

and corrosion [31], textile [17, 22], food packaging [22, 32-34], agriculture [35-37],

environmental and biomedical work [17, 36, 38]. When plasma is applied, a surface

will get the bombardment by amount of fast electrons, ions, and free radicals combine

in the UV-Vis spectrum. Lately, the atmospheric pressure plasma jet have showed

successful in present-day biomedical applications by reason of its capability on the

decontamination of biological and sterilization of various surfaces. Sterilization is

represent the process which all of the living microorganisms, including bacterial germs

are killed. Sterilization process can be accomplished by physical term, chemical term

and physiochemical term as show in figure1.

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Figure 1.1 Types of sterilization / disinfection method [39]

In physical sterilization applications, the popular traditional method is to use

dry or moist heat, such as an autoclave or steam sterilizer. However, these method still

have disadvantages. For example the use of autoclave can cause to a drenching and

wetting on sample, trapped air may reduce the efficacy. Also, it takes long time to cool.

In addition, the steam sterilization process typically takes minutes or hours. Despite of

the fact that heating provides a reliable way to eliminate the living microorganism, it is

not always suitable if it cause to damage heat-sensitive materials, such as electronics,

biological materials, many plastics, and fiber optics [40]. For these reasons chemicals

are also used for sterilization. In these situations, chemicals, both of gases or in liquid

phase, can be used as sterilants. The benefit of the use of gas and liquid chemical

sterilants can avoid the problem of heat damage[40], with the popular chemical are

alcohols, aldehydes, halogens, hydrogen peroxide and ethylene oxide, etc. [39-41].

Although, chemicals sterilization are an effective method. The drawback of

these chemicals were found for example skin irritant, volatile (evaporates rapidly),

inflammable were found in using alcohols. Aldehydes has poor penetration and also

leaves non-volatile residue. Halogen is rapidly inactivated in the presence of organic

matter. Hydrogen peroxide is decomposes in light. Ethylene oxide is highly toxic, also

highly flammable, irritating to eyes, skin, mutagenic and carcinogenic [39]. Plasma

sterilization becomes an alternative technique due to the fact that it can overcome these

problems. There are many researches and studies mention the benefit of using plasma

sterilization, for example a treatment of skin diseases and blood coagulation and wound

healing [43]. With the fact that plasma can produce a mixture of reactive agents, called

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“reactive oxygen-nitrogen species (RONS)” with the temperature of plasma remaining

close to feed gas temperature, it can result in a safe application to living cells and

tissues. These active plasma species consist of O, O3, NO, NO2 and OH radicals [42,

44-50]. The hydroxyl radical OH plays a key role as an oxidation agent in the

application area [34, 42, 51].

1.2 Statement of problem

There is a growing interest in the use of non-thermal plasmas in biomedicine.

Non-thermal, atmospheric pressure plasma sources are especially suitable for use with

heat-sensitive substrates. Due to having of the bulk temperature of the plasma close to

room temperature which can reduces the adverse effects of thermal loads on materials

such as living tissue. At the cellular level, there are many groups investigating plasma

sterilization in the laboratory. Bacterial spores can be killed after exposure to plasma

due to UV radiation, charged species and reactive neutrals. The reactive species work

to etch the cell until the cell membrane ruptures, and the UV radiation damages the

DNA.

The aim of this research is to develop atmospheric pressure plasma jet for the

use in bio-medical term by comparing the performance of threes designs of plasma jet

models and optimize the condition for generate the atmospheric plasma jet for using in

laboratory. In term to identify the performance of plasma jet, several models of plasma

jet are compared in the operating with the applied voltage at 2-3 kV, 2-7 kHz while

flowrate of argon gas was varied in range of 2-5 liter/minute. The measurement of the

three parameters including with plasma plume, power discharge of plasma and optical

emission spectrum were observed. In addition the effect of plasma on animal skin were

recorded by observation on the property changing, the wettability or water contact

angle.

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1.3 Scope of study

The 3 models of plasma jet are developed and study the effective by

1.4.1 Study the parameters which will effect on the power discharge and plasma plume

length by vary the frequency, applied voltage and gas flow rate.

1.4.2 Optimize the suitable model for use in the experiment.

1.4.3 Apply to treat on animal skin and observe the differentiation.

1.4 Significance of the study

1.5.1 This research study will develop and improve a model of plasma jet.

1.5.2 A model of plasma jet will use to treat with the other experimental in the future.

1.5.3 The results from this pilot study will be useful in term of study and development

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Chapter 2

Literatures Review

2.1 Basic and Fundamentals of plasma

Plasma is a fourth state of matters. Normally while the temperature increased,

the matter changes from a solid phase to a liquid phase. Then, it changes to a gas phase.

Finally, the gas is ionized and changes to plasma state [55, 56].

Figure 2.1. The state of matter [57]

Figure 2.2. Gas and plasma state: In a plasma, the gas’s electron were ionized

and ripped out from nucleus. The plasm state consists of charged particles both

negative and positive ions [58]

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Although the plasma state is nearly the gas state, their differences can be

summarized and described in the table 2.1.

Table 2.1 the differentiation between plasma state and gas state [48]

Plasma has characteristics which are different from other state (solid, liquid and

gas), with the three properties. The first is the debye length which is the length that the

charge was cover (shielding). From the basic principle of plasma, this length must be

much smaller than the distance between the plasmas. Second, the number of particles

in the debye sphere (ND) with a radius equal to the debye length must be a lot of

particles. Third, the periodic movement of plasma can be observed. When the plasma

is disturbed by external action potentials, the electrons, which are much less massive

ions, move out to the balance position and cause the plasma loosed electrical neutrality.

So, they return to the plasma electrically neutral as the original by the restoring force

acting on the electrons to move back to balance point. However, due to inertia, the

electrons will move beyond the balance. The shake around the balance point called

“The frequency of the plasma”. This shake is so quickly that the ions do not have

enough time to response to an electric field with is rapidly changing. Thus, it can

consider that the ions have a fixed position [59].

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2.1.1 Plasma Criteria

According to a difference characteristics of plasma when compare to other

states. It can be concluded the condition of plasma by following

2.1.1 Debye length ( λD << L)

When L is a length of the plasma dimensions, λD is a range that charged

particles in a plasma were shielded by the opposite charge.

2.1.2 The number of particles ND within a Debye sphere with a radius

λD must be very high ( ND >> 1).

2.1.3 Electricity neutral or nearly neutral conditions (Quasi – neutron).

2.1.4 The frequency of collisions between electrons and neutron

particles is less than the natural frequency of plasma [59].

Irving Langmuir was the first scientist who discovered the plasma in 1928[60]

in his experiment also found the plasma in the natural such as lightning or polar light

in the Arctic and Antarctic, solar wind and earth ionosphere[55, 56]. Moreover, from

natural plasma can be observed in human artifact such as neon lamp.

Figure 2.3 Plasma in nature [61]

When the plasma occurs and touches with the surface as a state of solid

materials such as metallic or plastic, the energy of plasma transfers to the surface of the

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material. Then, it will cause to properties change such as surface energy.

Many manufacturing industries used this principle for improving surface because

plasma energy will make the change in term of increase more wettability of material's

surface [62].

Figure 2.4 Plasma application for surface modification

Source: http://www.plasmatreat.com

2.2 Generation of plasma

Plasma can created by providing the sufficient energy feed directly to a gas

which purpose to make the rearrange of the specie’s electron structure such as

molecules or atoms. It leads to the creation of electrons or negative charged particles

and ions or positive charged particles. When the system operated and the applied

voltage is sufficient, the electric breakdown of the gas occurs. The air, for example, can

be ionized and leads to the conducting path which a current can flow. This process we

call “discharge”. Normally the inert gases, such as Argon (Ar), helium (He), Xenon

(Xe), and Neon (Ne) are used to serve the working gas for plasma generation. The

discharge can be applied to any situation where a gas is ionized by an electric field. A

current flow the energy will transcribe to the gas electrons. After that, it will transferred

to the species of the neutron by the collisions. Consider follow the probabilistic laws, it

can be divided the collisions into two groups:

Elastic collisions: the neutron specie’s internal energy do not change only give

a little effect slightly higher the kinetic energy.

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Inelastic collisions: the collision will change the structure of the electronic

inside the neutral species and make the generation of the excited species or

generation of the ions when the high of the electronic energy is sufficient.

For the excited species, most of them have a short lifetime. They emit a photons

when they fallen to the ground state which can be observed by human eyes. When the

plasma spreads throughout to the surrounding air, it will become a gas state by

recombination of the ions and electrons [63, 64].

2.2.1 Ionization processes

The energy that provides to the atoms of different elements in the gaseous phase to

strips out the electrons orbit around the atoms by feeding the energy called “Ionization

Energy”. This Ionization energy depends on the number of electrons of the atoms.

When the gas is ionized, it becomes a conductive media. In order to make the ionization,

the electron energy must exceed the ionization potential of the atom [64].

Ionization is the basic process in plasma because this process serves to

responsible for its creation and existence. This process includes two types of the

ionization. First is the direct ionization which comes from the impact of electrons.

Another one is the ionization which includes non-excited atoms such as radicals or

molecules, related with the interaction of an electrons. This electrons has enough

energetic crash with among of other neutral species with the conditions that all of these

neutral species have a high energy to create an ion – electron pairs.

To generate plasma by applying breakdown potential exceeding voltage of a gas

can be described by Paschen’s law [65], which mentions about the relationship between

the breakdown voltage VB and the gas pressure p and the distance d. In dry air with the

distance between the electrodes at 1 cm, operating at atmospheric pressure requires the

breakdown approximately 30 kV of DC voltage. If the distance d reduces from 1cm to

1 mm, the breakdown voltage reduces to 3.2 kV[65]. In Argon and Helium gases which

have an inert gas properties, it requires about 1.5 kV and 0.75 kV, respectively [63].

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Figure 2.5 the approximate of breakdown voltage for several gas follow the

Paschen’s law [63, 65]

2.3 Plasma categories

Plasma normally classified into 2 groups. Thermal plasma and Non - Thermal

plasma. According to the relationship between the temperatures of the electrons also

the changing of plasmas properties like electrons density. The table below shown the

main characteristic between Thermal plasma and Non – Thermal plasma [63, 66].

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Figure 2.6 The assortment of plasma state (in term of the temperature of

electrons versus the density of electrons) [63]

Table 2.2 Represent the major characteristics between Thermal plasma and

Non- thermal plasma [63]

According to the generation of plasma when the energy was given to system, it

can cause an electron movement and then leads to collisions. When inelastic collisions

occur between the electrons and the heavy particles, these can cause the generation of

active species, free radicals in plasma. Elastic collisions, however serve to increase the

heat both of electrons and heavy particles. For this reason in Thermal plasma state, the

electrons temperature (Te), heavy particles temperature (Th) and the gas temperature in

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the overall (Tg) are in the same range. However, another state of plasma, Non- Thermal

plasma state, the electrons temperature (Te) is different from the previous state because

in this state electrons temperature has much more higher than the heavy particles’s

temperature (Th) [67].

2.3.1 Thermal Equilibrium Plasma or Thermal Plasma

In thermal equilibrium plasmas, radiative processes and collisions will control

the transitions and reactions of the chemical. Collisions phenomena are reversible

process. It means that the excitations process is together with the de-excitation process.

The ionization process is together with the recombination process. In order to come up

at thermal equilibrium state, the local gradients of plasma properties such as density of

electron, thermal conductivity and temperature must low sufficient to make the particle

inside the plasma get up to the equilibrium. Between the heavy particles and the electron

particles inelastic collisions phenomena generated the active species inside plasma

while the heavy particles and electrons were heated up by elastic collisions phenomena.

This is the reason that make the electron’s temperature (Te), heavy particle’s

temperature (Th) and the overall temperature of the gas (Tg) are almost the same [67].

2.3.2 Non Thermal Equilibrium Plasma or Non Thermal plasma

In non-thermal plasma equilibrium state, the heavy particle’s temperature (Th)

is much lower than the electrons temperature (Te) due to the differentiation of mass

between the heavy particles and electrons. The temperature of plasma or the

temperature of the gas (Tg) is dominated by the heavy particle’s temperature i.e. Te>>Th

Tg. The deviation of non-thermal plasma from Boltzman distribution for the electrons’

density could be described by the truth that the electron induced de-excitation rate of

atoms is lower than the corresponding electron induced excitation rate because of

significant radiative de-excitation rate. The moving of electrons is very fast while the

heavy particles seem to static when compare with the electrons. Unlike thermal

equilibrium plasma, the local gradients of plasma properties in non-thermal plasma

state should be high enough and diffusion time should be less than the time. The

particles need to reach the equilibrium. Inelastic collisions between electrons and the

heavy particles are responsible for plasma chemistry, whereas only a few elastic

collisions heat up the heavy particles slightly (Th ≈ 300 - 1000 K). This is the reason

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why the electrons have highly energetic (Te≈ 10,000- 100,000 K). In non-thermal

plasma the whole plasma temperature remain low (cold plasma) [67].

Atmospheric pressure plasma

The drawback of producing plasma is the limit of low pressure and cost of

maintenance the pump and system. Thus, atmospheric pressure plasma can overcome

this problem. The below figure shows the relationship between the temperature,

pressure and their effects on the nature of the plasma [68]. From the figure it can be

seen if the pressure parameter increases, both electrons and heavy particle temperatures

will change. The entire system of plasma shifts from non-thermal plasma state (cold

plasma) to thermal plasma state. In zone which both of temperature and pressure are

lower (approximately 10-3 to 10-1 Torr), the temperature of electron (Te) is higher than

gas temperature (Tg).

Figure 2.7 Show the effect between the temperatures and pressure on the nature

of the plasma [68]

The plasma in chemistry is mostly related to the inelastic collision phenomena

which occur between the heavy particles and the electrons at the lower conditions of

pressure and temperature. This phenomena cannot increase the plasma’s termperature

or the heavy particle’s temperature. When the pressure increases, it leads to the

reduction of the temperature difference between electron particles and heavy particles.

Both of inelastic collisions process and elastic collisions process serve to increase the

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heavy particle’s temperature get intensified and plasma reaches close to the

thermodynamic equilibrium. Plasma at atmospheric pressure is almost thermal

equilibrium plasma [63, 68]. The atmospheric pressure plasma can be derived into 2

zones as:

A center of plasma device zone is thermal equilibrium state.

Roundabout of the center zone which is non-thermal equilibrium state. In this

term heavy particle’s temperature is lower than temperature of electrons

particles.

2.4 Atmospheric pressure plasma jet

The structure of plasma jet devices includes two electrodes. It can be found that

there are several design of electrodes either one electrode connect with power; while

another electrode is grounded or is ignored. Some designs look like plasma jet with the

inner and outer tube; while inner tube is used to ignite the plasma and another tube

serves to be a precursor tube [47]. A diagram of an atmospheric-pressure plasma jet is

shown in figure 2.8

Figure 2.8 the schematic of plasma jet

The generation of the discharge normally occurs inside a Pyrex tube. Around

the Pyrex glass tube, a ring shape ground electrode is placed. High purities of Argon

gas is used in our system for the plasma generation.

The plasma form between the two electrodes will spread outside of the Pyrex

glass in form of plasma jet due to the air/gases flowrate which feed to the structure as

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mention in above from structure as shown in Figure 2.8 The ionized gas from the

plasma jet with operated at ambient air exits through a nozzle.

2.5 Free radicals

Free radicals or reactive species refer as unstable molecules, or atoms due to

its contain of unpaired electrons which can form bonds with other substances. This

process is called “oxidation”. Radicals can have positive, negative or neutral charges.

Normally it comes from external sources such as cigarette smoking, air pollutants,

chemical industrial and x-rays [45, 69].

Figure 2.9 the source of free radicals [70]

When oxidation in the body occurs it will lead to cause the degeneration of the

body. The radicals both of the reactive oxygen species (ROS) and the reactive nitrogen

species (RNS) are well known that they are in great numbers and easily to generate at

the ambient air condition. The lists of example such as hydroxyl radical (OH-),

hydrogen peroxide (H2O2), and nitric oxide (NO-) [44, 71]. Presently, the advantage of

the reaction product from these species are well known that it has strongly oxidative

characteristics that can trigger signaling pathways in living cells. These species has a

key roles in cell growth, metabolism and physiology, immune responses, aging and

several other cell processes [44, 72].

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Many literatures review that those radicals ROS have a very short of lifetime

and yield the high toxic to the cells in result to damage of other cells and molecules

structures. For examples, oxidation of the lipids and proteins that constitute the

membrane of biological cells causes to the loss of their functions. In such plasma-

induced environments bacterial cells were found to die in minutes or even seconds [45].

In plasma chemistry and plasma medicine the hydroxyl radical (OH-) plays an

important role due to a higher oxidation potential and stronger disinfection power

compared to other oxidative species [73]. These plasma applications have been become

more alternative applied for inactivation both of gram-positive and gram-negative

bacteria the list is shown on the table below.

Table 2.3 List of the bacteria both of Gram – negative and Gram positive with

report that APPJ can effect [46]

Seiji Kanazawa and colleagues studied and measure OH radicals by chemica

luse dosimetry method [74]. They also studied the effect of treatment distances on OH

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radical production and observed that if the distances increased the amount of OH would

decrease.

Figure 2.10 Effect of treatment distances on OH radical production [74]

2.6 Design of atmospheric plasma jet

In order to treat with living tissue, thermal damage need to be avoid. Plasma jet

can be used to treat without thermal damage or electrical shock because it can generate

a rich, dry chemistry in air at ambient temperature, such as reactive oxygen and nitrogen

species.

There are various type of plasmas in the research fields. Many designs were

reviewed. X Lu and colleagues, for example, studied and reviewed the various designs

of the development of plasma jet [66] and they reported that there are a designs which

can be launch a several centimeter plasma plume, mainly consist of two electrodes and

one dielectric. When the high voltage power supply is turned on and a working gas is

supplied, which usually be Argon and Helium, into the dielectric tube, atmospheric

pressure plasma jet can be generated. The various designs of plasma jet are shown in

the figure 2.11.

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Figure 2.11 Schematic of atmospheric plasma jet (A) HV electrode not covered

by the dielectric, (B) HV electrode covered by the dielectric tube. (C) The two

ring electrodes are attached to the surface of two centrally perforated dielectric

disks [66]

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2.7 Current research of atmospheric plasma jet

In plasma state, it consists of high energy particles like ions and electrons which

can transfer energy to molecules or atoms of matter that contact with plasma. It causes

the change of position or change of bond and makes the new creation of rearrange of

atoms. Plasma jets devices are largely used in applications of plasma processing.

Especially, the plasma jet is used for surface treatments: cleaning of surface, surface

etching, surface coating and surface activation. Also Plasma jets have also been used

for medical in term of sterilization or bacterial inactivation. Below is some literatures

that review and mention the application of plasma.

V. Sarron and colleagues studied the plasma plume length characterization by

used the plasma gun which was based on a dielectric barrier discharges. The main

parameters considered were pulse width, applied voltage also the gas flowrate, which

found to have effects to the plasma plume length. The result shown that if the gas

flowrate has increasing the plume will increase until the critical point [24] this result

look similar in thesis work.

K. Shimizu and colleagues set up the experiment using micro plasma, composed

with a two of metallic electrodes, covered with a dielectric barrier under the operated

low discharge voltage of around 1kV to remove the low concentration of formaldehyde

in the ambient air. Formaldehyde (HCHO) is one of the most common VOCs indoors

which come from resins, plastics and building materials such as plywood. HPLC is the

method to measure the concentration of formaldehyde. They found that the removal

efficiency when applied discharge voltage approximate 1 kV is about 50% without

humidity on the other hand if consider the humidity factor the result shown that 60% of

humidity has more efficiency than treat without the humidity [75].

Božena Šerá and colleagues studied and observed the growth of the buckwheat

(Fagopyrum aesculentum) and its germination after exposed with plasma discharge

using air at low- temperature with the reckon time at 180 s, 300 s and 600 s. All of the

samples were six days incubated at 20 °C of temperature with dark conditions. Three

parameters, including the germination rate, the length, and the weight of sprout were

recorded and analyzed with two-way ANOVA method. The result showed that the

plasma technique can be effect to the buckwheat by increase of the germination rate

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higher than traditional at 9 % after treat with plasma. Also, the length of sprouts was

founded 7% higher with 600s plasma treatment [76].

R. Shrestha and colleagues studied and used the plasma jet in order to inactivation

the prokaryotic cells (Escherichia coli, Staphylococcus aureus) and eukaryotic cells

(Candida albicans, Saccharomyces cerevisae). By using Colony Forming Unit the

results shown that >4 log10 reduction in E. coli and < 2,000 cells reduction in

eukaryotic microalgae C. vulgaris with operated at 27 kHz of frequency, 3.5 kV of

voltage and 2 SLPM argon gas flow rate [77].

Mounir Laroussi and colleagues have been studied the effects of the plasma

pencil which operated at the atmospheric pressure on prokaryotic microorganisms

(bacteria) and cancer cells. They found the positive result that plasma can effect on

bacterias and cancer cells by using counting colony forming unit method also making

the measurement the size of the inactivation zone after2 minutes exposure with the

plasma [46].

Tanaka and colleagues presented a short review describing the interaction of

cancer cells with non-thermal at atmospheric pressure plasma. They outlined recent

innovative studies suggesting that the cold atmospheric plasma can affected on cells

both directly and indirectly. They were no negative effects of plasma on healthy cells,

such as fibroblasts and epithelial cells. This result is appropriate for wound healing

applications [78-80].

A. Hassan and colleagues applied cold plasma on polymers for improve surface

properties of polymers, which is an important requirement for industrial and high

technological applications. In this case, they use Mylar and Makrofol to be a sample

both of samples were cleaned by for 15 minutes then rinsed the samples with distilled

water and make it dry in air for long time before the samples get the interaction by the

RF-plasma source. For the measurement, the static contact angle (SCA) was determined

by using sessile drop method. The droplets of distilled water were inserted on the

sample surface by using a micropipette. They captured an image by using CCD camera

and analyzed by using the Image J software. The result showed that the wettability both

of Mylar and Makrofol decrease [79-81].

Hyun Jung Lee and colleagues studied the effect of atmospheric pressure

plasma jet on the Listeria monocytogenes and processed meat surfaces. The breast and

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ham were used to study. The He, N2 (both 7 L/min) mixed with 0.07 L/min of O2 were

used to produce the plasma. The exposure time was 2min. The result showed that after

treatment, the number of L. monocytogenes reduced. Inaddition, the reduction of

aerobic bacteria on the meat surface were found [72, 82].

Zifan Wan and colleagues were study the inactivation of Salmonella and its

effect on the egg quality by using an atmospheric pressure plasma jet. A medium A

grade eggs were purchased from local store whereas the Salmonella enterica serovar

Enteritidis (strain 190:88) was obtained from Department of Food and science. 0.1 ml

SE inoculum was spot inoculate on the sideway of eggs. Then, the eggs were allowed

to air dry for 1 hour in a laminar flow cabinet at room temperature to allow attachment

of bacterial cells. After drying, the eggs were placed in a refrigerator at 5 ̊C for

overnight to reach treatment temperature prior to the plasma treatment. The eggs were

treated with high voltage about 85 kV. A reduction of 5.3 log cfu/egg was observed

[83].

N.N. Misra and colleagues. Applied cold plasma on strawberries, which

purchased from the local store fruit market and treated by atmospheric cold plasma.

The plasma were generated with a 60 kV dielectric barrier discharge (DBD) pulsed at

50 Hz, across a 40 mm electrode gap, generated inside a sealed package containing

ambient air (42% relative humidity). The result showed the background microflora

(aerobic mesophillic bacteria, yeast and mould) of strawberries treated for 5 min was

reduced by 2 log10 within 24 hours [32].

A.-Young Moon et al. (2016) studied and developed atmospheric pressure

plasma source to remove the microorganisms on fruit product. In this case, the grapes

were trialed. By using flow of plasma gas with avoiding direct contact between plasma

and grapes. The observation was recorded and found that the fungi colony density of

the non-treated case consistently increased from 2.07 to 4.24 Log CFU/g. While the

fungi colony density of the plasma treated air case rapidly decreased from 2.07 to 0.7

Log CFU/g for 12 hours and then gradually increased. This indicates that atmospheric-

pressure air plasmas can hamper the growth of fungi and improve storage quality

through initial decontamination [33].

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Alison Lacombe and colleagues used atmospheric pressure plasma to kill the

microorganism on blueberries. Blueberries were obtained from the local grocery store

and held at 4° C until use to treat with plasma treatment. 5 blueberries were treated

under plasma working distance of 7.5 cm for 0, 15, 30, 45, 60, 90, or 120s. All

treatments with atmospheric pressure plasma significantly (P < 0.05) reduced after

exposure, with reductions ranging from 0.8 to 1.6 log CFU/g and 1.5 to 2.0 log CFU/g

compared to the control after 1 and 7 days, respectively [34].

Walsh and Kong reported on the frequency effects of plasma bullets. They

performed experiments from 10 to 500 kHz and found three different types of plasma

dynamics. When the frequency increased from 80 kHz to 170 kHz, the plasma appeared

much longer and brighter. They attributed this to the increased current density at higher

frequencies [84].

2.8 Brief information on the animal skin

2.8.1 Information about Skin

The largest organ of our body is the skin contains of a complex structure’s layer,

which forms and serve to a barrier play an important role such as protection our body

from environmental aggressions (biologic, physical or chemical), thermoregulation,

metabolism and sensation. Normally our skin was divided into 3 layers, the outermost

layer or epidermis, the middle layer or dermis and the inmost layer or hypodermis or

subcutaneous. The epidermis layer, consists of a specific constellation of cells known

as keratinocytes, which function to synthesize a long keratin with a protective role. The

middle layer is the dermis layer, basically made up of the fibrillar structural protein

which we known as collagen. The dermis layer is on top of the subcutaneous tissue, or

panniculus, which contains small lobes of fat cells known as lipocytes. The thickness

of these layers varies considerably, depending on the geographic location on the

anatomy of the body.

The epidermis usually is divided into four layers according to keratinocyte

morphology and position as they differentiate into horny cells, including the basal cell

layer (stratum germinativum), the squamous cell layer (stratum spinosum), the granular

cell layer (stratum granulosum), and the cornified or horny cell layer (stratum

corneum). The dermis is an integrated system of fibrous, filamentous, and amorphous

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connective tissue that accommodates stimulus-induced entry by nerve and vascular

networks, epidermally derived appendages, fibroblasts, macrophages, and mast cells.

Other blood-borne cells, including lymphocytes, plasma cells, and other leukocytes,

enter the dermis in response to various stimuli as well. The last one subcutaneous layer,

this layer functions as a storehouse of energy [85] [86].

Figure 2.12 Show the main structure of skin

2.8.1.1 Porcine skin

Due to the size of swine is comparable to humans, and both species share many

similarities in their cardiovascular and immune systems [87]. As in humans skin,

porcine skin is also divided into three layers, the epidermis, the dermis and the

hypodermis (or subcutaneous) [88].

Pork’s skin is similar to human’s skin in term of epidermal thickness and

dermal–epidermal thickness ratios, a basement membrane zone forming the interface

between the epidermis and the dermis. It constitutes a support to epidermal cells that

plays a crucial role in the polarity of growth and cytoskeleton organization of basal

epidermal. Pork’s skin are almost hairless and has a fixed subcutaneous layer and

dermal hair follicles like humans skin and look like to be thicker on the dorsum of the

neck than in humans and also less vascular in all areas. Porcine skin consist of clearly

basophilic granules along the basal layer. There are few eccrine sweat glands, and this

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among of eccrine glands make the different between porcine skin and human skin.

Porcine skin have apocrine sweat glands and sebaceous glands throughout the skin [77].

Figure 2.13 Comparative histological aspect of porcine (A) and human (B) skin

(haematoxylin-eosin-saffron staining). HF: hair follicle, Mu and arrowhead: arrector

pili muscle, SwG: Sweat gland, SG: sebaceous gland, Ad: Adipocytes (hypodermis) [82]

2.8.1.2 Structure of porcine skin

Porcine skin can be shown on Figure 2.13 Porcine skin have pH about 6-7 while

humans skin have pH approximately 5. The epidermis layer was described as 70-140

µm and composed of the following layers from outside to inside: stratum corneum,

stratum lucidum, statum granulosum, stratum spinosum and stratum basale. The

epidermis ends at the epidermal-dermal junction. The cellular turnover rate in the skin

is approximately 28-30 days, which is similar to humans [89].

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Figure 2.13 Histologic section of pig skin from the abdomen.

H&E staining 10X

Reference: M. Michael Swindle, 2008 [90]

2.8.1.3 Structure of the cell membrane

The plasma membrane allows nutrients and waste to travel to and from the cell,

while maintaining the structural integrity of the cell and sensing changes in the external

environment. It is made up of a lipid bilayer and proteins, each accounting for about

50% of the weight of the membrane. The lipids form the basic structure, while the

proteins distributed throughout the membrane are responsible for cell communication,

recognition and adhesion. The lipid bilayer is arranged with the hydrophilic heads

facing out, and the hydrophobic tails forming the interior of the wall [51].

2.9 Wettability

Characteristic of wet or liquid adhesion to the surface of solids is one of

important properties for skin study. The wettability of surfaces involves the two forces.

1. Cohesive force: the bond between the same substances. In this case, it is an

attempt to force the molecules of liquid form into each cluster.

2. Adhesive force: the bond between different substances. It refers to molecular

forces between liquid and solid. This is the force that opposites to the cohesive

force. Adhesion tries to make the trickle of liquid separate from each other.

Consider as the water settles on the surface of the solid state cause balance the

adhesion strength and cohesive strength. If the adhesion force is greater than an

extremely cohesive force, water distribution is attached on the surface of the solid. On

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the other hand, if the adhesion force is less than the cohesive force connection. Water

is not adhesion, but it falls out of the surface. Then, it combines into drops and roll back

and forth on the surface of solids, like a drop of water on a lotus leaf [91].

In consideration of the wetting liquid on the surface of solids, the balance of the

two forces leads to another significant quantity. Commonly used measure of wet which

called contact angle is the angle between a drops of liquid and the surface. The contact

angle between 0 – 90 degree can define as a good wetting or “Hydrophilic”. Whereas

contact angle between upper from 90 degree can define as “Hydrophobic”[92]. This

information can be used to define the wet of water on the surface.

Figure 2.14 Contact angles for hydrophobic and hydrophilic surfaces [92]

Figure 2.15 Different between hydrophobic (right) and hydrophilic (left) [92]

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Chapter 3

DEVELOPMENT OF ATMOSPHERIC PLASMA JET

3.1 Three designs of plasma jet and power supply system

In this thesis, it starts from deign a three designs of plasma jet with have slightly

difference and optimize each design. Plasma jet is usually consist of two electrodes (at

least one of them covered with a dielectric material). In figure 3.1-3.3, it shows for each

design considered. The first design has a tip shape at the exist end, shown in figure 3.1.

In figure 3.2, it shows the schematic of design 2. In figure 3.3, the design 3 uses the

glass same size with design 2 and add another part that is the capillary tube to cover an

inner electrode.

Figure 3.1 shown the schematic of plasma jet design 1(The end of the tube has a

nip shape)

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Figure 3.2 shown the schematic of plasma jet design 2

Figure 3.3 shown the schematic of plasma jet design 3 (Dielectric covered an

inner electrode)

In this work, copper ring was used as an outer electrode while the stainless steel was

used as an inner electrode. The conditions used to investigate and recorded performance

of three designs of atmospheric pressure plasma jet are varying the three parameters

voltage, flowrate and frequency. The range of voltage used was 2-3 kV, while the range

of argon gas flowrate was 2-5 liter/minute and the frequency was 2-7 kHz. For each

designs with three times repetition per day. This experimental were conducted for 5

days.

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3.2 Structure of atmospheric plasma source APPJ

Figure 3.4 the experiment set up in the laboratory

Lists of component of atmospheric pressure plasma jet

3.1.1 AC power supply which can generate the high voltage in range 1- 40 kV,

Frequency in range 50 – 100 kHz

3.1.2 Plasma device

3.1.2 High voltage probe Peak tech 1:

3.1.4 Oscilloscope Rigol DS1052E 50MHz 1GSa/S model.

3.1.5 Camera Nikon D5100 model

3.1.6 Optical Emission Spectrum Device

Power supply system

The power supply starts with a function generator to send a sine signal to the

amplifier. The amplifier receives the signals and then amplifies the input signal and

sends the signal in from of output signal to the ignition coil car, which serves to increase

the voltage to rise to apply on electrodes of plasma jet system.

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Function Generator

A waveform signal generator can produce several types of waves, sine waves,

square waves. Triangle wave, etc., can be the source of signal frequency between about

1 Hz to 10 MHz for trial use the signal generator MCP model SG1638N was used in

the experiment, as shown in figure 3.5.

Figure 3.5 Function generator MCP SGN1638N model

Amplifier

An amplifier is used to increase the amplitude of the input signal from the

function generator and prepare to be forwarded to the output signal sent to the ignition

coil car. The main purpose of the amplifier is to amplify the signal to a more size. And

the signal must be minimum distortion [93]. MOSFET MKII 2 0 0 W model was used

in the experiment.

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Figure 3.6 Power amplifier Power MOSFET MKII 200 W model

Ignition coil

An ignition coil is used for an extension of voltage to reach up high voltage.

Inside the ignition coils, it is composed of primary coil, wrapped around a steel core

with a large copper wire by 150 to 300 turns cover the secondary coil, which is a small

copper wire wrapped around an iron core approximately 2000 turns. In order to protect

short circuit between these coils, the thin paper was placed in the middle. Also it

contains the oil inside for make the cooling. One end of the primary coil is connected

to the positive (+) and the other end is connected to the negative (-) of the ignition coil.

For the secondary coil, it is connected at one end to a positive (+) of the ignition coil

and the other end is connected to the high voltage power pole in the middle of ignition

coils for high-voltage lines on the cap. The ignition coil HANSHIN E301 model was

used in the experiment.

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Figure 3.7 Schematic of ignition coil

Source from http://www.motorera.com/dictionary/co.htm

3.3 Parameters to identify the performance of plasma jet

3.3.1 Measurement of power discharge

The power consumption is significant for all plasma applications[33]. A. Janeco

and colleagues studied and gave the information that “A common method for measuring

the power consumption is by calculating the area of a Lissajous figure which obtained

from the applied voltage and integrated charge through a capacitor and computing the

area of the figure.” [94, 95].

The oscilloscope displays and records an electric signal such as the applied

voltage feed into plasma jet system or the voltage signal which across the capacitor.

This is used to analyze the power discharge. To measure the power discharge, in the

experiment the oscilloscope was used to measure the high voltage (Vmax) and Vc or

voltage across the capacitor and use Q = CVc to calculate the charge result. Follow the

below equations;

Equation (1)

P = W

t When P = power discharge (Watt)

W = work (J)

t = time (s)

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Equation (2)

2

T t

2T t

0

0

dt i(t)v(t) W

When Q = CV Vc= Voltage across C

Equation (3)

dt

dVC

dt

dQ i(t)

c,

Equation (4)

2

T t

2T - t

2T t

2T - t

c

0

0

0

0

dQ(t) v(t) dVC v(t) W

From the oscilloscope, the Lissajouses graph between applied voltage and

charge on capacitor can be obtained. The numerical technique in MATLAB is used to

estimate the plasma discharge power.

Figure 3.8 some result of discharge power from MATLAB software calculation

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3.3.2 Measurement of plasma plume length

In experiment, the camera was used for recorder the plasma plume length. It is

found that the plasma output when it lunches out to the ambient air. It has a conical

shape with purple colour, depending on type of working gas. The below figure shown

the shape of plasma output in each design with Air and Argon as working gas. The left

hand side are the output plasma the first design, the second design, and the third design

respectively.

Figure 3.9 the conical shape of plasma jet on each design

3.3.3 Optical Emission spectroscopy characteristic

In nature, plasma is considered as a quasi-neutral charge. It consists of the

mixture of highly active species which plays an important role on the substrate

treatment. In order to confirm the existent of these active species, the optical emission

spectroscopy (OES) is used. OES is well known as non-disturbing and non-invasive

technique for plasma diagnostics. In plasmas, excitation and de-excitation processes

keep going on. When molecules reach de-excite state, they emit radiations, which the

OES can capture these emission radiations of active species. The intensity of emission

radiation is measured as the function of the wavelength. As all of the transitions taken

placed at very specific wavelengths, these spectrum can be used to identify different

active species[67].

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Chapter 4

RESULTS AND DISCUSSION

4.1 Discharge power of plasma jet

The relation between the plasma power and voltage also the frequency of each

designs for different flow-rate is shown in figure 4.1 - 4.3.

It can be seen that as either the voltage or frequency increases, the power

discharge increases. However, it seems to be more sensitive an increase of the supplied

voltage. This result agrees with the report by Attri, P and colleagues [96]. However the

flow rate, seems to have no impacts on the discharge power of atmospheric plasma.

4.1.1 Discharge power of design 1

In order to obtain the discharge power, the equation (4) in the previous chapter

was used to calculate by using MATLAB software. The result for each design are

showed as in figure 4.1 – 4.3.

Figure 4.1 The plasma discharge power obtained from a jet system design 1 is

shown as a function of applied frequency at different applied potential

0

500

1000

1500

0 1 2 3 4 5 6 7 8

Dis

char

ge P

ow

er

(mw

)

Frequency (kHz)

2lpm-2kV 3lpm-2kV4lpm- 2kV 5lpm- 2kV2lpm- 3kV 3lpm - 3kV4lpm - 3kV 5lpm - 3kV

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4.1.2 Discharge power of design 2

Figure 4.2 The plasma discharge power obtained from a jet system design 2 is

shown as a function of applied frequency at different applied potential

0

500

1000

1500

0 1 2 3 4 5 6 7 8

Dis

char

ge p

ow

er (

mw

)

frequency (kHz)

2lpm - 2kV 3lpm -2kV

4lpm -2kV 5lpm -2kV

2lpm -3kV 3lpm -3kV

4lpm -3kV 5lpm - 3kV

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4.1.3 Power discharge of design 3

Figure 4.3 The plasma discharge power obtained from a jet system design 3 is

shown as a function of applied frequency at different applied potential

4.2 Plasma plume length of plasma jet

The plasma plume length was observed by using camera and the result showed

that the plasma plume length will increase if the apply voltage increase. In this work

The second design yields the highest length with 1.8 cm when compare with the other

designs

The correlation method was used to analyze and found that it has a relation for

each other with agreeable to the literature reviews that the increasing of high voltage,

frequency and gas flow should be increase of the plasma plume[97]. The similarity

reviews mentioned that the plasma plume length will increase until it reach up the

critical value [24].

0

500

1000

1500

0 1 2 3 4 5 6 7 8

Dis

char

ge p

ow

er

(mw

)

Frequency (kHz)

2lpm -2kV 3lpm -2kV4lpm -2kV 5lpm - 2 kV2lpm -3kV 3lpm -3kV4lpm -3kV 5lpm -3kV

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4.2.1 Plasma plume length of design 1

Figure 4.4 The result of plasma plume length of design 1 shown as a function of

applied frequency whereas the applied potential was fixed at 2kV at any flow

rate range 2-5 liter/minute

0

0.5

1

1.5

2

0 2 4 6 8

Pla

sma

plu

me

len

gth

(cm

)

Frequency (kHz)

2lpm-2kV 3lpm-2kV

4lpm- 2kV 5lpm- 2kV

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Figure 4.5 The result of plasma plume length of design 1 shown as a function of

applied frequency whereas the applied potential was fixed at 3kV at any flow

rate range 2-5 liter/minute

The result shown that both of frequency and flowrate do not effect on the plasma

plume length. For this design 1 at condition 2kV, 2- 7 kHz and 2-5 liter/minute the

maximum plasma plume length approximately 0.4 cm. When operate at the condition

3kV, 2- 7 kHz and 2-5 liter/minute shown the same trend lined that frequency and

flowrate do not effect on the plasma plume length for this result after increase the

applied potential from 2kV to 3kV the maximum plasma plume is approximately 1.0

cm.

0

0.5

1

1.5

2

0 2 4 6 8

Pla

sma

plu

me

len

gth

(cm

)

Frequency (kHz)

2lpm- 3kV 3lpm- 3kV

4lpm- 3kV 5lpm - 3kV

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4.2.2 Plasma plume length of design 2

Figure 4.6 The result of plasma plume length of design 2 shown as a function of

applied frequency whereas the applied potential was fixed at 2kV at any flow

rate range 2-5 liter/minute

Figure 4.7 The result of plasma plume length of design 2 shown as a function of

applied frequency whereas the applied potential was fixed at 3kV at any flow

rate range 2-5 liter/minute

0

0.5

1

1.5

2

2.5

3

0 1 2 3 4 5 6 7 8

Pla

sma

plu

me

len

gth

(cm

)

frequency (kHz)

3lpm -2kV 4lpm -2kV

5lpm -2kV 2lpm - 2kV

0

0.5

1

1.5

2

2.5

3

0 2 4 6 8

Pla

sma

plu

me

len

gth

(cm

)

frequency (kHz)

2lpm -3kV

3lpm -3kV

4lpm -3kV

5lpm - 3kV

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The result from design 2 also give the same notice with the previous design that

the flow rate and frequency do not effect on plasma plume length. And for this design

2 when operated at 2kV, 2- 7 kHz and 2-5 liter/minute Argon gas flow rate is

approximately 0.8 cm. When operate at the condition 3kV, 2- 7 kHz and 2-5 liter/minute

shown the same trend lined that frequency and flowrate do not effect on the plasma

plume length for this result after increase the applied potential from 2kV to 3kV the

maximum plasma plume is approximately 1.7 cm.

4.2.3 Plasma plume length of design 3

Figure 4.8 The result of plasma plume length of design 3 shown as a function of

applied frequency whereas the applied potential was fixed at 2kV at any flow

rate range 2-5 liter/minute

0

0.5

1

1.5

2

0 1 2 3 4 5 6 7 8

Pla

sma

plu

me

len

gth

(cm

)

Frequency (kHz)

2lpm -2kV 3lpm -2kV

4lpm -2kV 5lpm - 2 kV

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Figure 4.9 The result of plasma plume length of design 3 shown as a function of

applied frequency whereas the applied potential was fixed at 3kV at any flow

rate range 2-5 liter/minute

The result from design 3 also give the same notice with the two previous designs

that the flow rate and frequency do not effect on plasma plume length. It ‘s founded tht

only the applied voltage is When operate at the condition 3kV, 2- 7 kHz and 2-5

liter/minute shown the same trend lined that frequency and flowrate do not effect on

the plasma plume length for this result after increase the applied potential from 2kV to

3kV the maximum plasma plume is approximately 1.7 cm.

0

0.5

1

1.5

2

0 1 2 3 4 5 6 7 8

Pla

sma

plu

me

len

gth

(cm

)

Frequency (kHz)

2lpm -3kV 3lpm -3kV

4lpm -3kV 5lpm -3kV

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Table 4.1 The summary results of plasma jet in three designs

The plasma performance based on three designs of atmospheric plasma jet

system are investigated. It was found that after varying the three parameters voltage,

flow rate and frequency. The first design yields the highest power discharge among

those designs considered. When the supplied voltage, the frequency, and Argon flow-

rate, the similar trend has been observed from those three designs. For the plasma plume

length term, Design 2 yields the highest plume length due design 2 has more diameter

at the end of the pyrex glass tube when compare with design 1 which has a narrow

shape at the end of the pyrex glass tube. When the working gases in this system, Ar and

Air feed into the design 1. The working gases will accumulate nearly the end at the tube

and when the plasma launch out it will rapid mixing to the ambient air. Thus, the plasma

plume length sound to shorter than the atmospheric plasma design 2.

4.3 Optical Emission Spectroscopy of plasma jet

Power discharge

Design 1 Design 2 Design 3

1405 ± 156 mW 989 ± 416 mW 484 ± 69 mW

Plasma plume

length

1.0 ± 0 cm 1.8 ± 1 cm 0.3 ± 0 cm

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Figure 4.10 The emission spectra of the plasmas produced with Argon gas, top

figure is shown at the applied potential 2kV. Bottom figure is shown at the

applied potential 3kV

For the emission spectra obtained from plasmas jet with Argon gas (Ar) flow.

Emissions from the OH radical which formed from the water vapor in ambient air

(around 306,307,308,309,310) are present in the emission spectra [23, 74, 98-100].

Some N2 line emissions also present in the spectra. The emission spectra of Argon is

appear at the right end of spectra [23]. The results of OES spectrum represent the similar

trend lines in these 3 designs of plasma jet. The trend lines seem that Argon flow rate

2 liter/minute give the highest count when compare with the others. This result seem

agreeable with Boonyawan. D et al. (2016)[23]. With mention that The OH radical

emission light is quite intense at argon gas flow rate between 2 liter/minute to

3 liter/minute.

4.4 Impact of plasma jet on pork skin

The effect on pork skin was observed after 30 seconds and 60 seconds exposing

with atmospheric pressure argon plasma jet. The measurement was carried out using

the Image J software. The result of pork skins after treated with plasma for 30 seconds

and 60 seconds are show in below figure.

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Figure 4.11 Contact angle on pork skin. Without treated with plasma jet the

degree has higher than treated with plasma jet

After treating with atmospheric plasma on pork skin, the result shows that

contact angle decreases. It implies that atmospheric pressure plasma may not be

good for healing wound if it considers only this factor of wettability because the

wound will hard to dry. However, if only sterilization is considered, plasma jet

might be able to use for killing microorganisms due to the contact angle decrease.

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Chapter 5

Conclusions and Recommendations

5.1 Conclusions

In this work, it is founded that the power discharge depend on applied voltage

and frequency and it is does not depend on the Argon flow rate. For the plasma plume

length, the results show that there are no effect on plume although the applied voltage,

frequency and the flow rate were varied when analyzed by ANOVA method. The

further study is try to analyzed the effect on plasma plume by used Correlation method

and it was found that they have a relation between the applied voltage and the frequency

come up with the flow rate. Moreover, the observation of the OES spectrum was

recorded and the trend line for each design is look similar. When consider the effect of

three parameters, it is founded that the trend line will increase if the applied voltage and

the frequency increase. For the term of the flow rate variation, it is founded that the

flow rate at 2lpm give the most count when compare to another flow rates. Thus, the

suitable design in the laboratory should be the first design with the condition 3kV 7

kHz and 2 liter/minute. For the effect of plasma on pork skin, it is observed in term of

the wettability or water contact angle. The result shows that the contact angle decreases

after the explosion with plasma or the pork’s skin increases its hydrophilic property.

5.2 Recommendation

It is recommended that a dielectric of the plasma jet should be replaced the

quartz glass instead of Pyrex glass because the quartz has more heat resistance.

Currently the Pyrex always crack when it exposes with the high applied voltage. For

this case the plasma jet system will available to operate at the high voltage more than

the traditional system.

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Appendix

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Appendix

Results Information

The results of power discharge for each 5 days in each design are showed in

the following.

1. The power discharge of design 1 at the condition 2-3 kV, 2-7 kHz and 2-5 lpm

day1

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2. The power discharge of design 1 at the condition 2-3 kV, 2-7 kHz and 2-5 lpm

day2

3. The power discharge of design 1 at the condition 2-3 kV, 2-7 kHz and 2-5 lpm

day3

4. The power discharge of designl 1 at the condition 2-3kV, 2-7 kHz and 2-5 lpm

day4

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5. The power discharge of design 1 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day5

6. The power discharge of design 2 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day1

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7. The power discharge of design 2 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day2

8. The power discharge of design 2 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day3

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9. The power discharge of design 2 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day4

10. The power discharge of design 2 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day5

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11. The power discharge of design 3 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day1

12. The power discharge of design 3 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day2

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13. The power discharge of design 3 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day3

14. The power discharge of design 3 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day4

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15. The power discharge of design 3 at the condition 2-3kV, 2-7 kHz and 2-5 lpm day5

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ANOVA METHOD : Effect of voltage on power discharge design 1

Sum of Squares df Mean Square F Sig.

Fre2kHz2lpm Between Groups .003 1 .003 46.227 .000

Within Groups .000 8 .000

Total .003 9

Fre2kHz3lpm Between Groups .003 1 .003 129.584 .000

Within Groups .000 8 .000

Total .003 9

Fre2kHz4lpm Between Groups .003 1 .003 63.873 .000

Within Groups .000 8 .000

Total .004 9

Fre2kHz5lpm Between Groups .003 1 .003 156.440 .000

Within Groups .000 8 .000

Total .003 9

Fre3kHz2lpm Between Groups .006 1 .006 70.555 .000

Within Groups .001 8 .000

Total .007 9

Fre3kHz3lpm Between Groups .005 1 .005 149.151 .000

Within Groups .000 8 .000

Total .005 9

Fre3kHz4lpm Between Groups .006 1 .006 91.032 .000

Within Groups .001 8 .000

Total .007 9

Fre3kHz5lpm Between Groups .006 1 .006 72.839 .000

Within Groups .001 8 .000

Total .007 9

Fre4kHz2lpm Between Groups .013 1 .013 94.509 .000

Within Groups .001 8 .000

Total .014 9

Fre4kHz3lpm Between Groups .012 1 .012 125.257 .000

Within Groups .001 8 .000

Total .012 9

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Fre4kHz4lpm Between Groups .011 1 .011 94.938 .000

Within Groups .001 8 .000

Total .012 9

Fre4kHz5lpm Between Groups .013 1 .013 110.505 .000

Within Groups .001 8 .000

Total .014 9

Fre5kHz2lpm Between Groups .024 1 .024 69.541 .000

Within Groups .003 8 .000

Total .027 9

Fre5kHz3lpm Between Groups .022 1 .022 111.022 .000

Within Groups .002 8 .000

Total .023 9

Fre5kHz4lpm Between Groups .021 1 .021 80.179 .000

Within Groups .002 8 .000

Total .024 9

Fre5kHz5lpm Between Groups .023 1 .023 96.850 .000

Within Groups .002 8 .000

Total .025 9

Fre6kHz2lpm Between Groups .029 1 .029 70.358 .000

Within Groups .003 8 .000

Total .032 9

Fre6kHz3lpm Between Groups .031 1 .031 71.604 .000

Within Groups .003 8 .000

Total .035 9

Fre6kHz4lpm Between Groups .032 1 .032 149.800 .000

Within Groups .002 8 .000

Total .033 9

Fre6kHz5lpm Between Groups .033 1 .033 70.716 .000

Within Groups .004 8 .000

Total .036 9

Fre7kHz2lpm Between Groups .042 1 .042 106.747 .000

Within Groups .003 8 .000

Total .046 9

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Fre7kHz3lpm Between Groups .046 1 .046 107.226 .000

Within Groups .003 8 .000

Total .049 9

Fre7kHz4lpm Between Groups .053 1 .053 161.510 .000

Within Groups .003 8 .000

Total .055 9

Fre7kHz5lpm Between Groups .045 1 .045 69.240 .000

Within Groups .005 8 .001

Total .050 9

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ANOVA METHOD : Effect of voltage on power discharge design 2

Sum of Squares df Mean Square F Sig.

Fre2kHz2lpm Between Groups .002 1 .002 9.680 .014

Within Groups .001 8 .000

Total .003 9

Fre2kHz3lpm Between Groups .002 1 .002 10.058 .013

Within Groups .001 8 .000

Total .003 9

Fre2kHz4lpm Between Groups .002 1 .002 13.697 .006

Within Groups .001 8 .000

Total .003 9

Fre2kHz5lpm Between Groups .002 1 .002 3.747 .089

Within Groups .004 8 .000

Total .006 9

Fre3kHz2lpm Between Groups .004 1 .004 13.896 .006

Within Groups .002 8 .000

Total .006 9

Fre3kHz3lpm Between Groups .004 1 .004 13.821 .006

Within Groups .002 8 .000

Total .006 9

Fre3kHz4lpm Between Groups .004 1 .004 10.039 .013

Within Groups .003 8 .000

Total .007 9

Fre3kHz5lpm Between Groups .001 1 .001 1.229 .300

Within Groups .006 8 .001

Total .007 9

Fre4kHz2lpm Between Groups .008 1 .008 12.620 .007

Within Groups .005 8 .001

Total .013 9

Fre4kHz3lpm Between Groups .006 1 .006 11.015 .011

Within Groups .005 8 .001

Total .011 9

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Fre4kHz4lpm Between Groups .007 1 .007 11.202 .010

Within Groups .005 8 .001

Total .012 9

Fre4kHz5lpm Between Groups .003 1 .003 1.373 .275

Within Groups .015 8 .002

Total .018 9

Fre5kHz2lpm Between Groups .014 1 .014 13.753 .006

Within Groups .008 8 .001

Total .022 9

Fre5kHz3lpm Between Groups .012 1 .012 12.019 .008

Within Groups .008 8 .001

Total .021 9

Fre5kHz4lpm Between Groups .013 1 .013 10.906 .011

Within Groups .009 8 .001

Total .022 9

Fre5kHz5lpm Between Groups .004 1 .004 1.286 .290

Within Groups .028 8 .003

Total .032 9

Fre6kHz2lpm Between Groups .021 1 .021 12.412 .008

Within Groups .013 8 .002

Total .034 9

Fre6kHz3lpm Between Groups .019 1 .019 11.470 .010

Within Groups .013 8 .002

Total .032 9

Fre6kHz4lpm Between Groups .018 1 .018 10.970 .011

Within Groups .013 8 .002

Total .031 9

Fre6kHz5lpm Between Groups .005 1 .005 .831 .389

Within Groups .045 8 .006

Total .049 9

Fre7kHz2lpm Between Groups .027 1 .027 11.938 .009

Within Groups .018 8 .002

Total .046 9

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Fre7kHz3lpm Between Groups .021 1 .021 13.971 .006

Within Groups .012 8 .002

Total .033 9

Fre7kHz4lpm Between Groups .024 1 .024 13.269 .007

Within Groups .015 8 .002

Total .039 9

Fre7kHz5lpm Between Groups .005 1 .005 .859 .381

Within Groups .047 8 .006

Total .052 9

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ANOVA METHOD : Effect of voltage on power discharge design 3

Sum of Squares df Mean Square F Sig.

Fre2kHz2lpm Between Groups .000 1 .000 16.937 .003

Within Groups .000 8 .000

Total .000 9

Fre2kHz3lpm Between Groups .000 1 .000 17.138 .003

Within Groups .000 8 .000

Total .000 9

Fre2kHz4lpm Between Groups .000 1 .000 174.509 .000

Within Groups .000 8 .000

Total .000 9

Fre2kHz5lpm Between Groups .000 1 .000 169.945 .000

Within Groups .000 8 .000

Total .000 9

Fre3kHz2lpm Between Groups .000 1 .000 15.117 .005

Within Groups .000 8 .000

Total .001 9

Fre3kHz3lpm Between Groups .000 1 .000 17.002 .003

Within Groups .000 8 .000

Total .001 9

Fre3kHz4lpm Between Groups .001 1 .001 141.425 .000

Within Groups .000 8 .000

Total .001 9

Fre3kHz5lpm Between Groups .001 1 .001 191.693 .000

Within Groups .000 8 .000

Total .001 9

Fre4kHz2lpm Between Groups .001 1 .001 14.749 .005

Within Groups .000 8 .000

Total .001 9

Fre4kHz3lpm Between Groups .001 1 .001 16.763 .003

Within Groups .001 8 .000

Total .002 9

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Fre4kHz4lpm Between Groups .001 1 .001 151.151 .000

Within Groups .000 8 .000

Total .002 9

Fre4kHz5lpm Between Groups .001 1 .001 322.808 .000

Within Groups .000 8 .000

Total .001 9

Fre5kHz2lpm Between Groups .001 1 .001 14.085 .006

Within Groups .001 8 .000

Total .002 9

Fre5kHz3lpm Between Groups .002 1 .002 14.058 .006

Within Groups .001 8 .000

Total .003 9

Fre5kHz4lpm Between Groups .003 1 .003 79.484 .000

Within Groups .000 8 .000

Total .003 9

Fre5kHz5lpm Between Groups .003 1 .003 202.152 .000

Within Groups .000 8 .000

Total .003 9

Fre6kHz2lpm Between Groups .003 1 .003 17.082 .003

Within Groups .001 8 .000

Total .004 9

Fre6kHz3lpm Between Groups .003 1 .003 17.077 .003

Within Groups .001 8 .000

Total .004 9

Fre6kHz4lpm Between Groups .003 1 .003 233.233 .000

Within Groups .000 8 .000

Total .004 9

Fre6kHz5lpm Between Groups .003 1 .003 147.267 .000

Within Groups .000 8 .000

Total .003 9

Fre7kHz2lpm Between Groups .003 1 .003 12.615 .007

Within Groups .002 8 .000

Total .005 9

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Fre7kHz3lpm Between Groups .003 1 .003 17.626 .003

Within Groups .001 8 .000

Total .005 9

Fre7kHz4lpm Between Groups .005 1 .005 206.589 .000

Within Groups .000 8 .000

Total .005 9

Fre7kHz5lpm Between Groups .005 1 .005 189.806 .000

Within Groups .000 8 .000

Total .005 9

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ANOVA METHOD : Effect of frequency on power discharge design 1

Sum of Squares df Mean Square F Sig.

volt2kV2lpm Between Groups .008 5 .002 26.651 .000

Within Groups .001 24 .000

Total .009 29

volt2kV3lpm Between Groups .007 5 .001 44.894 .000

Within Groups .001 24 .000

Total .007 29

volt2kV4lpm Between Groups .007 5 .001 33.940 .000

Within Groups .001 24 .000

Total .008 29

volt2kV5lpm Between Groups .006 5 .001 42.135 .000

Within Groups .001 24 .000

Total .007 29

volt3kV2lpm Between Groups .073 5 .015 34.704 .000

Within Groups .010 24 .000

Total .083 29

volt3kV3lpm Between Groups .077 5 .015 41.134 .000

Within Groups .009 24 .000

Total .086 29

volt3kV4lpm Between Groups .083 5 .017 54.511 .000

Within Groups .007 24 .000

Total .090 29

volt3kV5lpm Between Groups .071 5 .014 28.562 .000

Within Groups .012 24 .000

Total .082 29

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ANOVA METHOD : Effect of frequency on power discharge design 2

Sum of Squares df Mean Square F Sig.

volt2kV2lpm Between Groups .003 5 .001 5.768 .001

Within Groups .002 24 .000

Total .005 29

volt2kV3lpm Between Groups .004 5 .001 6.236 .001

Within Groups .003 24 .000

Total .006 29

volt2kV4lpm Between Groups .003 5 .001 4.558 .005

Within Groups .003 24 .000

Total .006 29

volt2kV5lpm Between Groups .014 5 .003 .844 .532

Within Groups .077 24 .003

Total .090 29

volt3kV2lpm Between Groups .042 5 .008 4.404 .005

Within Groups .046 24 .002

Total .088 29

volt3kV3lpm Between Groups .036 5 .007 4.482 .005

Within Groups .039 24 .002

Total .075 29

volt3kV4lpm Between Groups .036 5 .007 4.089 .008

Within Groups .043 24 .002

Total .079 29

volt3kV5lpm Between Groups .027 5 .005 1.941 .125

Within Groups .067 24 .003

Total .095 29

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ANOVA METHOD : Effect of frequency on power discharge design 3

Sum of Squares df Mean Square F Sig.

volt2kV2lpm Between Groups .001 5 .000 60.190 .000

Within Groups .000 24 .000

Total .001 29

volt2kV3lpm Between Groups .001 5 .000 21.844 .000

Within Groups .000 24 .000

Total .001 29

volt2kV4lpm Between Groups .001 5 .000 49.979 .000

Within Groups .000 24 .000

Total .001 29

volt2kV5lpm Between Groups .001 5 .000 50.555 .000

Within Groups .000 24 .000

Total .001 29

volt3kV2lpm Between Groups .008 5 .002 7.530 .000

Within Groups .005 24 .000

Total .013 29

volt3kV3lpm Between Groups .006 5 .001 6.890 .000

Within Groups .004 24 .000

Total .011 29

volt3kV4lpm Between Groups .008 5 .002 56.724 .000

Within Groups .001 24 .000

Total .008 29

volt3kV5lpm Between Groups .007 5 .001 72.059 .000

Within Groups .000 24 .000

Total .008 29

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ANOVA METHOD : Effect of argon flowrate on power discharge design 1

Sum of Squares df Mean Square F Sig.

Volt2kV2kHz Between Groups .000 3 .000 .278 .840

Within Groups .000 16 .000

Total .000 19

Volt2kV3kHz Between Groups .000 3 .000 .269 .847

Within Groups .000 16 .000

Total .000 19

Volt2kV4kHz Between Groups .000 3 .000 .450 .721

Within Groups .001 16 .000

Total .001 19

Volt2kV5kHz Between Groups .000 3 .000 .951 .440

Within Groups .001 16 .000

Total .001 19

Volt2kV6kHz Between Groups .000 3 .000 .252 .858

Within Groups .001 16 .000

Total .001 19

Volt2kV7kHz Between Groups .000 3 .000 .615 .615

Within Groups .001 16 .000

Total .001 19

Volt3kV2kHz Between Groups .000 3 .000 .091 .964

Within Groups .001 16 .000

Total .001 19

Volt3kV3kHz Between Groups .000 3 .000 .283 .837

Within Groups .002 16 .000

Total .002 19

Volt3kV4kHz Between Groups .000 3 .000 .226 .877

Within Groups .003 16 .000

Total .003 19

Volt3kV5kHz Between Groups .000 3 .000 .192 .900

Within Groups .007 16 .000

Total .008 19

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Volt3kV6kHz Between Groups .000 3 .000 .095 .962

Within Groups .011 16 .001

Total .011 19

Volt3kV7kHz Between Groups .001 3 .000 .212 .886

Within Groups .014 16 .001

Total .014 19

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ANOVA METHOD : Effect of argon flowrate on power discharge design 2

Sum of Squares df Mean Square F Sig.

Volt2kV2kHz Between Groups .001 3 .000 1.191 .345

Within Groups .002 16 .000

Total .003 19

Volt2kV3kHz Between Groups .001 3 .000 1.077 .387

Within Groups .003 16 .000

Total .004 19

Volt2kV4kHz Between Groups .002 3 .001 1.216 .336

Within Groups .008 16 .001

Total .010 19

Volt2kV5kHz Between Groups .003 3 .001 1.140 .363

Within Groups .016 16 .001

Total .020 19

Volt2kV6kHz Between Groups .005 3 .002 .991 .422

Within Groups .027 16 .002

Total .032 19

Volt2kV7kHz Between Groups .007 3 .002 1.255 .323

Within Groups .028 16 .002

Total .035 19

Volt3kV2kHz Between Groups .001 3 .000 .604 .622

Within Groups .005 16 .000

Total .006 19

Volt3kV3kHz Between Groups .000 3 .000 .081 .969

Within Groups .010 16 .001

Total .010 19

Volt3kV4kHz Between Groups .000 3 .000 .024 .995

Within Groups .022 16 .001

Total .022 19

Volt3kV5kHz Between Groups .000 3 .000 .009 .999

Within Groups .037 16 .002

Total .037 19

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Volt3kV6kHz Between Groups .000 3 .000 .032 .992

Within Groups .057 16 .004

Total .058 19

Volt3kV7kHz Between Groups .001 3 .000 .061 .979

Within Groups .063 16 .004

Total .064 19

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ANOVA METHOD : Effect of argon flowrate on power discharge design 3

Sum of Squares df Mean Square F Sig.

Volt2kV2kHz Between Groups .000 3 .000 .763 .531

Within Groups .000 16 .000

Total .000 19

Volt2kV3kHz Between Groups .000 3 .000 2.406 .105

Within Groups .000 16 .000

Total .000 19

Volt2kV4kHz Between Groups .000 3 .000 2.174 .131

Within Groups .000 16 .000

Total .000 19

Volt2kV5kHz Between Groups .000 3 .000 3.718 .033

Within Groups .000 16 .000

Total .000 19

Volt2kV6kHz Between Groups .000 3 .000 2.181 .130

Within Groups .000 16 .000

Total .000 19

Volt2kV7kHz Between Groups .000 3 .000 2.985 .062

Within Groups .000 16 .000

Total .000 19

Volt3kV2kHz Between Groups .000 3 .000 .356 .786

Within Groups .000 16 .000

Total .000 19

Volt3kV3kHz Between Groups .000 3 .000 .682 .576

Within Groups .000 16 .000

Total .001 19

Volt3kV4kHz Between Groups .000 3 .000 .326 .806

Within Groups .001 16 .000

Total .001 19

Volt3kV5kHz Between Groups .000 3 .000 .427 .737

Within Groups .002 16 .000

Total .002 19

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Volt3kV6kHz Between Groups .000 3 .000 .124 .945

Within Groups .003 16 .000

Total .003 19

Volt3kV7kHz Between Groups .000 3 .000 .175 .912

Within Groups .004 16 .000

Total .004 19

The results of power plasma plume length for 5 days for each design are showed

in the following.

16. The plasma plume length of design 1 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day1

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17. The plasma plume length of design 2 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day2

18. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day3

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19. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day4

20. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day5

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21. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day1

22. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day2

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23. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day3

24. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day4

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25. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day5

26. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day1

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27. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day2

28. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day3

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29. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day3

30. The plasma plume length of design 3 at the condition 2-3 kV, 2-7 kHz and 2-5

lpm day5

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Corelation result : Between voltage versus frequency and flowrate of design 1

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Corelation result : Between voltage versus frequency and flowrate of design 2

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Corelation result : Between voltage versus frequency and flowrate of design 3

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The results of power plasma plume length for 5 days for each design are showed

in the following.

31. The OES result of plasma jet design 1 in condition 2kV, 2lpm and 2–7 kHz

at Day 1

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32. The OES result of plasma jet designl 1 in condition 2kV, 2lpm and 2–7 kHz

at Day 2

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33. The OES result of plasma jet design 1 in condition 2kV, 2lpm and 2–7 kHz

at Day 3

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34. The OES result of plasma jet design 1 in condition 2kV, 2lpm and 2–7 kHz

at Day 4

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35. The OES result of plasma jet design 1 in condition 2kV, 2lpm and 2–7 kHz

at Day 5

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36. The OES result of plasma jet design 1 in condition 3kV, 2lpm and 2–7 kHz

at Day 1

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37. The OES result of plasma jet design 1 in condition 3kV, 2lpm and 2–7 kHz

at Day 2

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38. The OES result of plasma jet design 1 in condition 3kV, 2lpm and 2–7 kHz

at Day 3

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39. The OES result of plasma jet design 1 in condition 3kV, 2lpm and 2–7 kHz

at Day 4

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40. The OES result of plasma jet design 1 in condition 3kV, 2lpm and 2–7 kHz

at Day 5

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41. The OES result of plasma jet design 2 in condition 2kV, 2lpm and 2–7 kHz

at Day 1

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42. The OES result of plasma jet design 2 in condition 2kV, 2lpm and 2–7 kHz

at Day 2

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43. The OES result of plasma jet design 2 in condition 2kV, 2lpm and 2–7 kHz

at Day 3

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44. The OES result of plasma jet design 2 in condition 2kV, 2lpm and 2–7 kHz

at Day 4

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45. The OES result of plasma jet design 2 in condition 2kV, 2lpm and 2–7 kHz

at Day 5

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46. The OES result of plasma jet design 2 in condition 3kV, 2lpm and 2–7 kHz

at Day 1

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47. The OES result of plasma jet design 2 in condition 3kV, 2lpm and 2–7 kHz

at Day 2

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48. The OES result of plasma jet design 2 in condition 3kV, 2lpm and 2–7 kHz

at Day 3

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49. The OES result of plasma jet design 2 in condition 3kV, 2lpm and 2–7 kHz

at Day 4

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50. The OES result of plasma jet design 2 in condition 3kV, 2lpm and 2–7 kHz

at Day 5

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51. The OES result of plasma jet design 3 in condition 2kV, 2lpm and 2–7 kHz

at Day 1

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52. The OES result of plasma jet design 3 in condition 2kV, 2lpm and 2–7 kHz

at Day 2

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53. The OES result of plasma jet design 3 in condition 2kV, 2lpm and 2–7 kHz

at Day 3

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54. The OES result of plasma jet design 3 in condition 2kV, 2lpm and 2–7 kHz

at Day 4

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55. The OES result of plasma jet design 3 in condition 2kV, 2lpm and 2–7 kHz

at Day 5

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56. The OES result of plasma jet design 3 in condition 3kV, 2lpm and 2–7 kHz

at Day 1

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57. The OES result of plasma jet design 3 in condition 3kV, 2lpm and 2–7 kHz

at Day 2

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58. The OES result of plasma jet design 3 in condition 3kV, 2lpm and 2–7 kHz

at Day 3

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59. The OES result of plasma jet design 3 in condition 3kV, 2lpm and 2–7 kHz

at Day 4

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60. The OES result of plasma jet design 3 in condition 3kV, 2lpm and 2–7 kHz

at Day 5