Cold-hardy Grape Germplasm Collection, Geneva, NY Acquire Maintain Conserve Characterize Evaluate Distribute Vitis Genetic Diversity Tenturier cultivar Leaf senescence Geneva, NY cold hardy grapes ~ 1371 accessions On own roots ~ 68% are Vitis hybrid ~20 species Davis, CA cold tender grapes ~ 3000 accessions ~ half are Vitis vinifera - 45 species Vitis aestivalis 29 Vitis amurensis 19 Vitis cinerea 47 Vitis coignetiae 11 Vitis hybrid 929 Vitis labrusca 45 Vitis monticola 2 Vitis mustangensis 1 Vitis palmata 7 Vitis piasezkii 3 Vitis pseudoreticulata 2 Vitis riparia 80 Vitis romaneti 4 Vitis rupestris 36 Vitis spp. 24 Vitis vinifera 84 Vitis tilifolia 1 Vitis thunbergii 1 Vitis vulpina 16 Vitis X champinii 4 Vitis X doaniana 2 Vitis X novae-angliae 1 Vitis yenshanensis 2 Ampelopsis aconitifolia var glabra 1 Ampelopsis brevipedunculata 4 Cissus sp. 1 Total 1371 Acquire Developed selections from breeders Elmer Swenson 1913-2004 pioneering grape breeder who revolutionized grape growing in cold and short- seasoned regions Wild accessions through exploration Mexico Kazakhstan USA Vitis mustangensis, Alabama (new, disjunct population) I1_01_005_PI_588118_01 DESCRIPTOR No. of accessions Berry length 1026 Berry shape 980 Berry size 975 Berry weight 979 Berry width 1026 Berry flavor 973 Bloom date 1078 Brix 979 Budburst date ~1350 Cluster length 1023 Cluster weight 981 Cluster width 980 Digital images ~1800 Flesh color 979 Peduncle length 980 Seedlesness 951 Sex of flower 1245 Skin color 1031 Characterize and Evaluate Collaborative evaluations 1) Berry resveratrol concentration 2) Leaf resveratrol content 3) Crown gall susceptibility 4) Genome size in base pairs 5) Genome size in pico grams In progress 6) Phomopsis resistance (Dr. Wilcox, CU) 7) Powdery mildes resistance (Dr. Reisch, CU ) 8) Genetics of grape-pathogen interactions (Dr. Cadle-Davidson, GGRU) 9) Key Secondary Metabolites (Dr. Chen, CU) min 0 10 20 30 40 50 mAU 0 5 10 15 20 DAD1 C, Sig=365,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D) 365nm min 0 10 20 30 40 50 mAU 0 20 40 60 80 100 DAD1 E, Sig=525,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D) 525nm min 0 10 20 30 40 50 mAU 0 10 20 30 40 50 60 DAD1 A, Sig=280,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D) 280nm Plus Passport Data All information available at: http://www.ars-grin.gov Characterizations Molecular: SSR fingerprint of every vine Photo documentation Vitis sylvestris PI 157518 10) Virus Indexing and Therapy (Dr. Fuchs, CU) 0 500 1000 1500 2000 2500 3000 3500 4000 4500 5000 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 05 06 07 08 09 No accessions annually mean = 1262 Annual distribution of grape germplasm 1988-2009 Grape total accessions distributed = 27,766 Grape total orders = 1413 Grape orders per year mean = 64 range 14 - 145 Distribute Dormant Cuttings, Seeds, Pollen, Flowers, Fruit, Leaves Research and Collaborations Phylogenomic Analysis of Grapes • Resequencing • 27 EST-derived markers • 11,400 aligned base pairs • 304 accessions (North America, China, Europe) • Phylogeographic analysis • Evolutionary history • Assess genetic diversity • develop tools/markers for • Germplasm conservation/use • Collaboration • Horizon x Ill547-1 • Numerous disease response/ viticultural traits • Germplasm characterization • Marker assisted selection Functional Marker Development Vitis9KSNP Array Supported
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Cold-hardy Grape Germplasm Collection, Geneva, NYAcquire Maintain Conserve Characterize Evaluate Distribute Vitis Genetic Diversity
Tenturier cultivar
Leaf senescence
Geneva, NYcold hardy grapes~ 1371 accessions
On own roots~ 68% are Vitis hybrid
~20 species
Davis, CA cold tender grapes~ 3000 accessions
~ half are Vitisvinifera
- 45 species
Vitis aestivalis 29Vitis amurensis 19Vitis cinerea 47Vitis coignetiae 11Vitis hybrid 929Vitis labrusca 45Vitis monticola 2Vitis mustangensis 1Vitis palmata 7Vitis piasezkii 3Vitis pseudoreticulata 2Vitis riparia 80Vitis romaneti 4Vitis rupestris 36Vitis spp. 24Vitis vinifera 84Vitis tilifolia 1Vitis thunbergii 1Vitis vulpina 16Vitis X champinii 4Vitis X doaniana 2Vitis X novae-angliae 1Vitis yenshanensis 2Ampelopsis aconitifolia var glabra 1Ampelopsis brevipedunculata 4Cissus sp. 1
Total 1371
AcquireDeveloped selections from breeders
Elmer Swenson 1913-2004
pioneering grape breeder who revolutionized grape growing in cold and short-seasoned regions
Wild accessions through exploration
Mexico
Kazakhstan USA
Vitis mustangensis, Alabama (new, disjunct population)
I1_01_005_PI_588118_01
DESCRIPTOR No. of accessions
Berry length 1026
Berry shape 980
Berry size 975
Berry weight 979
Berry width 1026
Berry flavor 973
Bloom date 1078
Brix 979
Budburst date ~1350
Cluster length 1023
Cluster weight 981
Cluster width 980
Digital images ~1800
Flesh color 979
Peduncle length 980
Seedlesness 951
Sex of flower 1245
Skin color 1031
Characterize and Evaluate
Collaborative evaluations 1) Berry resveratrol concentration
2) Leaf resveratrol content
3) Crown gall susceptibility
4) Genome size in base pairs5) Genome size in pico grams
In progress6) Phomopsis resistance (Dr. Wilcox, CU)
7) Powdery mildes resistance (Dr. Reisch, CU)
8) Genetics of grape-pathogen interactions (Dr. Cadle-Davidson, GGRU)
9) Key Secondary Metabolites (Dr. Chen, CU)
min0 10 20 30 40 50
mAU
0
5
10
15
20
DAD1 C, Sig=365,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D)
365nmmin0 10 20 30 40 50
mAU
0
20
40
60
80
100
DAD1 E, Sig=525,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D)
525nmmin0 10 20 30 40 50
mAU
0
10
20
30
40
50
60
DAD1 A, Sig=280,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D)
280nm
Plus Passport DataAll information available at:
http://www.ars-grin.gov
CharacterizationsMolecular: SSR fingerprint of every vine
Photo documentation
Vitis sylvestris
PI 157518
10) Virus Indexing and Therapy (Dr. Fuchs, CU)
0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 05 06 07 08 09
No
acce
ssio
ns a
nnua
lly
mean = 1262
Annual distribution of grape germplasm 1988-2009
Grape total accessions distributed = 27,766 Grape total orders = 1413Grape orders per year mean = 64 range 14 - 145
Distribute
Dormant Cuttings, Seeds, Pollen, Flowers, Fruit, Leaves
Research and Collaborations
PhylogenomicAnalysis of Grapes
• Resequencing• 27 EST-derived markers• 11,400 aligned base pairs• 304 accessions (North America,
China, Europe)• Phylogeographic analysis• Evolutionary history• Assess genetic diversity• develop tools/markers for • Germplasm conservation/use
• Collaboration• Horizon x Ill547-1• Numerous disease response/
viticultural traits• Germplasm characterization• Marker assisted selection
Functional MarkerDevelopment
Vitis9KSNP ArraySupported
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