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Characterization of pleiotropic adult plant resistance loci to wheat diseases Caixia Lan, Ravi P Singh, Sybil Herrera-Foessel, Julio Huerta-Espino, Bhoja R Basnet, Evans S Lagudah* * CSIRO Plant Industry, Australia
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Characterization of pleiotropic adult plant resistance loci to wheat diseases

Aug 23, 2014

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Caixia Lan, CIMMYT
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Page 1: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Characterization of pleiotropic adult plant resistance loci to wheat

diseases

Caixia Lan, Ravi P Singh, Sybil Herrera-Foessel, Julio Huerta-Espino, Bhoja R Basnet, Evans S Lagudah*

* CSIRO Plant Industry, Australia

Page 2: Characterization of pleiotropic adult plant resistance loci to wheat diseases

► Introduction

► PAPR research in CIMMYT

► Breeding for PAPR in wheat

► Future plan

Contents

Page 3: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Wheat major diseases

Black (stem) rust Puccinia graminis

Yellow (stripe) rust Puccinia striiformis

Brown (leaf) rust Puccinia triticina

Powdery mildew Blumeria graminis

Page 4: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Resistance loci for wheat diseases � Seedling resistance gene (race-specific) and adult

plant resistance gene (non-race specific)

� Resistance genes:

72 leaf rust, 63 stripe rust, 58 stem rust and 47

powdery mildew

� Resistance QTL:

80 for leaf rust, 140 stripe rust, 114 powdery

mildew, stem rust?

Page 5: Characterization of pleiotropic adult plant resistance loci to wheat diseases

“Boom-and-Bust”: Race-specific genes Year Variety Resistance genes Released Breakdown Race Country Bread Wheat: Yecora 70 Lr1, 13 1970 1973 ? Mexico Tanori 71 Lr13, 17 1971 1975 ? Mexico Jupateco 73 Lr17, 27+31 1973 1977 TBD/TM Mexico Genaro 81 Lr13, 26 1981 1984 TCB/TB Mexico Seri 82 Lr23, 26 1982 1985 TCB/TD Mexico Baviacora 92 Lr14b, 27+31 1992 1994 MCJ/SP Mexico Lovrin derived lines Yr9 1971-1972 1985 CYR29 China

Moro Yr10 ? 2011 ? Canada Milan Yr17 ? 2006 134 E16 A+ Australia Chuanmai 42 Yr24/26 2004 2010 V26 China Opata 85 Yr27 1985 1996 Mex96.11 Mexico Pastor Yr31 ? 2008 Mex08.13 Mexico Lovrin 10 Pm8 1970s 1990-1991 ? China Kavkaz Sr31 1980s 1998 Ug99 Uganda Durum Wheat: Altar 84 Lr72 1984 2001 BBG/BN Mexico Jupare 2001 Lr72, 27+31 2001 2007 BBG/BP Mexico Khapstein/ 9*LMPG and Vernal

Sr13, Sr13+Sr9e ? 2009 TRTTF and JRCQC

Ethiopia

Page 6: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Pleiotropic adult plant resistance (PAPR) genes �  Lr34 [Syn.=Yr18=Sr57=Pm38=Ltn1=Sb1=Bdv1] chromosome 7DS (leaf rust, yellow rust, stem rust, powdery mildew, leaf tip necrosis, spot blotch, barley yellow dwarf virus )

�  Lr46 [Syn.=Yr29=Sr58=Pm39=Ltn2=Ts?] chromosome 1BL �  Lr67 [Syn.=Yr46=Sr55=Pm46=Ltn3] chromosome 4DL �  Lr68 [yellow rust, powdery mildew and stem rust?] chromosome 7BL �  Yr54 [leaf rust, powdery mildew and stem rust?] chromosome 2DL

Apav (susceptible)

Lr67

Lr68

Lr46

Lr34

Avocet (susceptible)

Formation of cell wall appositions (instead of hypersensitivity with NBS-LRR type race-specific genes)

Page 7: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Potential PAPR QTLs for rusts

1BS, 2AL, 2BS, 2DL, 5AL, 5BL, 6AL and 7BL

Page 8: Characterization of pleiotropic adult plant resistance loci to wheat diseases

CIMMYT PAPR research

► Norman Borlaug, 1950s

► Goes back to mid. 1970s and initiated by Sanjay

Rajaram (breeder) and Jesse Dubin (pathologist)

► Late 1980s: Breeding strategy to develop high

yielding varieties with near-immune resistance

►  Early 2000s: Genetics and mapping of resistance

genes

Page 9: Characterization of pleiotropic adult plant resistance loci to wheat diseases

PAPR QTL analysis in Avocet/Sujata

PVEs: QYL.cim-1AS explained 10.5-13.8% and 7.9-8.2% of stripe rust and leaf rust, respectively; QYL.cim-7BL explained 16.6-20.4% and 5.7-13.0% of for stripe rust and leaf rust, respectively Lr46/Yr29 and Lr67/Yr46 were also mapped in the population

Page 10: Characterization of pleiotropic adult plant resistance loci to wheat diseases

PAPR QTL analysis in Avocet/Francolin#1

►  Additional QTL have been detected on 1BL (Lr46/Yr29), 3BS (LR/Yr30) and 7DS (PVE:3.3-4.2 for LR)

►  Flanking makers to Lr16 and YrF can be used in MAS based on testing in 350 lines from 45th IBWSN

PVE: 17.8–27.9% PVE: 10.3–21.1%

Lan et al. 2014 Molecular Breeding, DOI: 10.1007/s11032-014-0075-6

Page 11: Characterization of pleiotropic adult plant resistance loci to wheat diseases

PAPR QTL analysis in Avocet/Quaiu #3

► QYr.tam-2D, explained 49-64% of total phenotypic variation and was designated as Yr54

► QYLr.tam-3D explained up to 6 and 7% of the phenotypic variance, respectively

► Known resistance genes Lr46/Yr29, Sr2/Yr30 and Lr42

Basnet et al. 2014 Molecular Breeding 33 (2):385-399

Page 12: Characterization of pleiotropic adult plant resistance loci to wheat diseases

PAPR QTL analysis in Avocet/Kenya Kongoni

LR: QLr.cim-1DS (Lr42, 6-21%), QLr.cim-2BL (20% in BV2011) and

QLr.cim-3BS (5-10%)

YR: QYr.cim-2BS (7-12%) and QYr.cim-5BL (6-8%)

PVE: LR (12-57%) and YR (25-35%) PVE: LR (5-13% ) and YR (10%)

Page 13: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Fine mapping

� Single QTL/gene mapping populations

� Minor QTL with fixed genetic background

populations

� Deletion produced by γ-ray

Page 14: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Identification of deletion mutants for Lr67/Yr46/Sr55/Pm46

► Mutagenesis by gamma-irradiation using a 60 Co source at ININ, Mexico.

► 4000 seed radiated ► Grow M1, individually harvest plants ► Grow M2 (2000 lines, 20 space planted plants/M2), identify

susceptible, harvest… ► M3, M4 plots ► 1 mutant was 15 bp deletion (M55), 1 mutant was 3bp deletion

(M157) and 3 mutants were complete deletion of Lr67 (M87, M147, M168)

Page 15: Characterization of pleiotropic adult plant resistance loci to wheat diseases

MAS in rust Genes Markers Type Cultivar Reference PAPR genes Lr34/Yr18/Pm38/Sr57 Lr34SNP STS, SNP Parula Lagudah et al., 2009 Lr46/Yr29/Pm39/Sr58 csLv46 CAPS Pavon 76 Lagudah ES, pers comm Lr46/Yr29/Pm39/Sr58 csLV46G22 CAPS not in Parula Lagudah ES, pers comm Lr67/Yr46/Pm46/Sr55 Lr67SNP SNP RL6077 Lagudah ES, pers comm Lr68 CSGS, cs7BLNLRR CAPS Parula Herrera-Foessel et al. 2012 Sr2/Yr30 csSr2 CAPS Pavon76 Mago et al. 2011 Sr2/Yr30/Lr? gwm533 SSR Quaiu#3 Basnet et al. 2014 Yr54 Xgwm301 SSR Quaiu#3 Basnet et al. 2014 HTAP genes Yr36 Gpc-B1 Glupro, ND?? Uauy et al. 2006 Yr39 Xwgp36, Xwgp45, Xgwm18, Xgwm11 RGA, SSR Alpowa Lin and Chen, 2007 Yr52 Xbarc182, Xwgp5258 RGA, SSR PI 183527 Ren et al. 2012 Yr59 Xwgp5175, Xbac32, Xbac182 RGA, SSR PI 178759, PI 660061 Chen XM, pers comm Seedling genes Lr16 Xgwm210, Xwmc661 SSR Francolin#1 Lan et al. 2014 Lr19/Sr25 Psy1Da-g_SNP, PSY-E SNP, SSR Misr#1 Lr21 D14 Talbert et al. 1994 Lr42 cfd15, wmc432 SSR Quaiu#3 Basnet et al. 2014 Lr47 PS10R/ PS10L, PS10R/PS10L2 Helguera et al. 2000 Lr51 S30-13L/AGA7-759R Helguera et al. 2005 Yr17 VENTRIU/LN2, URIC/LN2 Milan Helguera et al. 2003 Yr24/26 CYD15, Xgwm11 Chuanmai 42 Zakari et al. 2003 Yr41 Xgwm410, Xgwm374 SSR Chuannong 19 Luo et al. 2008 Yr43 Xwgp110, Xwgp103, Xbarc139 RGA, SSR ID0377S Cheng and Chen 2010 Yr44

XpWB5/N1R1, Xwgp100, Xgwm501 RGA, SSR Zak Cheng and Chen 2010

Yr50 Xgwm540, Xbarc1096, Xwmc47, Xwmc310 SSR

CH223 Liu et al. 2013 Yr60 Xwmc776,Xwmc313,Xwmc219 SSR Lal Bahadur Herrera-Foessel, pers comm

Page 16: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Breeding strategy of wheat rusts

Adapted cultivar X Sujata

Adapted cultivar F1 X

(Obregon)

(Batan/Toluca)

BC1 (400-500 plants per cross, MR)

(Obregon)

F2 (1000-1600 plants per cross, low to MR)

(Batan/Toluca)

Bulk selected

F7 (30-40 lines, yield and quality tests)

F3-F4 (400 plants per cross, grain characteristics )

F5-F6 (60-80 lines per coss, small plot, grain characteristics

(Obregon,Batan/Toluca)

Bulk selected

(Batan/Toluca)

(Obregon)

Pedigree

Self-crossing

MAS

Phenotype and MAS

Breeder for YT and EYT

Page 17: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Why do we use PAPR in breeding?

► Leads to resistance durability- good for farmers and donors’ investment

► Genes have pleiotropic genetic control on rusts, powdery mildew and some other diseases

► Field based selection simultaneously with other traits increases high genetic gains for multiple traits

Page 18: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Utilization of PAPR genes in breeding: challenges

► Small to intermediate effects of individual genes ► Dispersed presence of genes in different cultivars and

germplasm ► Field selection environment lacking uniform and high disease

pressure ► Need for growing larger population sizes for selection ► Difficulty in distinguishing small effect race-specific genes from

slow rusting genes (especially for resistance to yellow rust) ► Higher G x E interaction on the expression and effectiveness of

genes Despite numerous challenges, significant progress was made at

CIMMYT for resistance to all three rusts

Page 19: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Cd. Obregón 39 masl High yield (irrigated), Water-use efficiency, Heat tolerance, Leaf rust, Stem rust (not Ug99)

Toluca 2640 masl Yellow rust Septoria tritici

El Batán 2249 masl Leaf rust, Fusarium

Njoro, Kenya 2185 masl Stem rust (Ug99 group) Yellow rust

►  Targeted crosses for shuttle breeding made in 2006 and 1st group of populations planted in Kenya in 2008

►  2000 F3/F4 populations undergo Mexico-Kenya shuttle ►  High yielding, resistant lines derived from 1st group of Mexico-Kenya shuttle

distributed worldwide in 2011 and 2012 ►  Distribution of new materials to continue each year

Njoro, Oct. 2008

Breeding for PAPR in CIMMYT Mexico (Cd. Obregon-Toluca/El Batan)- Kenya International Shuttle Breeding

A five-year recurrent breeding cycle

Page 20: Characterization of pleiotropic adult plant resistance loci to wheat diseases

Future plan ► New PAPR gene discovery in bi-parental and

association mapping panels

► Fine mapping and cloning genes Lr68, Yr54, YrF,

YrSuj, SrND643 and SrSHA7/SrHaril, and QTL on

1AS and 3DC

► Understanding the PAPR gene mechanism

►  PAPR gene pyramiding and marker assistant

selection in wheat breeding

Page 21: Characterization of pleiotropic adult plant resistance loci to wheat diseases

China: Zhonghu He Xianchun Xia Zaifeng Li Fangping Yang Garry Rosewarne Ennian Yang Jin Feng Yelun Zhang

Acknowledgements Collaborators:

Norway: Morten Lillemo South Africa Zakkie Pretorius Kenya: Sridhar Bhavani India: Arun Kumar Joshi

Donors:

DRRW

Thank you

Global Wheat Program (GWP)