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b.tech Biotech III Yr i Sem_0

Mar 05, 2015

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BIOTECHNOLOGY SYLLABUS SCHEME OF INSTRUCTION AND EXAMINATION B.Tech. (Biotech) III YEAR -I Semester

SUBJECT CODE

SUBJECTS

PERIODS/ WEEK The / Lab

MARKS Sessional 30 30 30 30 30 30 25 25 25 255 Exams. 70 70 70 70 70 70 50 50 50 570

DURATION OF EXAM Hrs. 3 3 3 3 3 3 4 4 4

BTT.3.101 BTT.3.102 BTT.3.103 BTT.3.104 BTT.3.105 BTT.3.106 BTP.3.107 BTP.3.108 BTP.3.109

Protein Engineering and Enzyme Technology Bio-fluid Mechanics and Heat Transfer Probability and Statistics Plant and Animal Biotechnology Bio-reaction Engineering Fermentation Technology Bio-reaction Engineering Lab Fluid Mechanics and Heat Transfer Lab Fermentation Lab

4 4 4 4 4 4 4 4 4

ROTEIN ENGINEERING & ENZYME TECHNOLOGY

Subject code

: BTT.3.101

Sessional

: 30 : 70 : 3 Hrs

Periods / Week : 4 Nature of Examination : Theory UNIT- I PROTEIN STRUCTURE AND FUNCTIONS

Examination Exam Duration

Peptide bond- Structure, functions, physical and chemical properties, chemical synthesis of peptides; liquid phase and solid phase techniques; Proteins-classification & Biological functions; Physico-chemical properties, forces stabilizing protein structure-primary structure and its determination, helical-p-pleated structure; Ramachandran plot; super secondary structure, tertiary & quaternary structure; myoglobin and hemoglobin, Lysozyme, Ribonuclease A, Carboxy Peptidase, Chymotrypsin, Sickle cell hemoglobin; structure and functional relationship; Fibrous protein (Collagen).

UNIT-II PROTEIN BIOSYNTHESIS AND STRUCTURE PREDICTION Methods of protein isolation, purification and Quantification; large scale synthesis of proteins, design and synthesis of peptides, use of peptides in biology, methods of detection and analysis of proteins; examples of engineered proteins, protein design, and examples. Post translation modification, protein transport, Inhibitors of Protein Synthesis. Random, site directed catalytic affectivity; Structure prediction and modeling of proteins:

UNIT- III PRODUCTION AND APPLICATIONS OF ENZYMES Production and purification of crude Enzyme extracts from plant, animal and microbial sources; Methods of characterization of enzymes; development of enzymatic assays. Production of recombinant proteins (Insulin, Interleukin, Interferon) having therapeutic and diagnostic applications; Important commercial enzymes; Proteases; Amylases; Lipases; Cellulases; Pectinases; Isomerases and its applications in food, pharmaceutical and other industries; Enzymes for analytical and diagnostic purposes; Design of enzyme electrodes and their application as biosensors in industry health care and environment.

UNIT- IV MECHANISMS AND KINETICS OF ENZYME ACTION

Mechanisms of enzyme action; Concept of active site and energetics of enzyme substrate complex formation; Specificity of enzyme action; Kinetics of single substrate reactions; Turn over number; Estimation of Michaeli-Menten parameters; Multi substrate reaction mechanisms and kinetics; Types of inhibition - Allosteric regulation of enzymes; Deactivation of kinetics.

UNIT- V ENZYME IMMOBILIZATION & MASS TRANSFER EFFECTS IN IMMOBILISED ENZYME SYSTEMS Physical and chemical techniques for enzyme immobilization - adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., examples; Advantages and disadvantages of different immobilization techniques; Overview of applications of immobilized enzyme systems; Applications of enzymes in analysis; Analysis of Film and pore Diffusion Effects on kinetics of Immobilized Enzyme Reactions; Formulation of dimensionless groups and calculation of Effectiveness Factors.

EXAMINATION:

One question from each unit with internal choice

TEXT BOOKS: 1. Principles of Biochemistry- Lenhinger, David Nelson, W.H.Freeman, (2006). 2. Enzyme Technology- Palmer Trevor, E.W.P, (2004). 3. Fundamentals of Biochemistry by J.L. Jain, Chand (S.) & Co Ltd , India ((1999)). REFERENCE BOOKS:

1. Biochemistry- Voet and Voet J.G, 2nd edition, John C.Wiley & Sons (1994). 2. Biochemical Engineering James M. Lee eBook Version 2.2. ii (2001) by James M.Lee, Gerald Reed , Steve Taylor Third Edition, Academic Press.

3. Enzymes by Paul R. Mathewson Eagan Press Handbook Series (1998). 4. Biocatalysis - Fundamentals and Applications Edited by Bommarius, Andreas Sebastian;Riebel, Bettina R. Wiley-VCH (2004). 5. Biocatalysts and Enzyme Technology by Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer, Wiley (2005). 6. Enzyme Technology by Ashok Pandey, Collins and others, Springer, (2006).

BIO-FLUID MECHANICS AND HEAT TRANSFER Subject code : BTT.3.102 Sessional : 30 : 70 : 3 Hrs

Periods / Week : 4 Nature of Examination : Theory

Examination Exam Duration

UNIT-I Basic Concepts in Flow of Fluids: Introduction, Nature of fluid, Rheology of fluids -Newton's law of viscosity. Concept of Newtonian and non-Newtonian fluids- Different types of nonNewtonian fluids with examples in bioprocessing. Measurement of viscosity using extrusion rheometer, plate and cone viscometer, coaxial cylinder viscometer etc.

UNIT-II Flow field, Friction losses in laminar flow through a circular tube (Hagen-Poiseuille equation), Friction losses in turbulent flow (Fanning equation), Pumping of fluids Flow through pipes, average velocity, flow regimes, boundary layer concept. Laminar and turbulent flow -characterization by Reynold's number, pressure drop due to skin friction and form friction, friction factor chart, Hageri -Poiseuille equation.

UNIX-III Flow past immersed bodies: Definition of drag and drag coefficient. Friction in flow through beds of solids, Brief introduction to flow of compressible fluids. Flow measuring and monitoring systems- valves, bends, elbows, prevention of leaks, mechanical seals, stuffing box. Flow measuring devices-manometers, orifice meter, venture meter and rotameter. Brief description of Pumps and Blowers

UNIT-IV Basic Concepts in "Heat Transfer: Introduction and Mechanisms of heat transfer; Conduction heat transfer (through slab, cylinder & Sphere); Conduction through solids in series, Forced convection heat transfer inside pipes, Introduction to radiation heat transfer, Chilling and freezing of food and Biological materials. Heat .transfer correlations, and calculations, basic heat exchange equipment

UNIT-V Basic Concepts in Evaporation and Condensation: Introduction, Types of evaporation equipment and operation methods; Overall heat transfer coefficients in evaporators; simple material balances. Calculation methods for single effect evaporators, Evaporation of biological materials. Types of condensation, numerical problems and condensation equipment. EXAMINATION: One question from each unit with internal choice

TEXT BOOKS: 1. Unit operations in Chemical Engineering, W L McCabe & J C Smith, 6th Ed., McGraw Hill Intl. Ed.(2005). 2 Transport Processes and Unit Operations by Christie J. Geankoplis, India Pvt. Ltd. 3rd Ed., Prentice Hall

3. Basic Fluid Mechanics, Kothandaraman C P and Rudramoorthy. R., New Age International Publishers, New Delhi, (1998). 4. Fundamentals of Engineering Heat and Mass Transfer by Sachdeva. R.C., New Age International Publishers. New Delhi, (1996)

REFERENCE BOOKS:1. Introduction to Chemical Engineering by S. K. Ghosal, S. K. Sanyal and S. Dutta, TMH 2.

3. 4. 5. 6. 7.

publications, (1993). Computational Fluid Mechanics and Heat Transfer, by Richard Pletcher Second Edition, Pub: Taylor & Francis; (1997) "Mechanics of Fluids". By Irving H Shames. 3rd Ed., McGraw-Hill Publishing Co.. New York, (1992). Rate Processes in Metallurgy, Mohanty A.K., Prentice Hall of India, New Delhi, (2000) Transport Phenomena by Byron Bird R., Stewart.W.E., John-Wiley & Sons Inc., (1994). Engineering Thermofluids: Thermodynamics, Fluid Mechanics, and Heat Transfer By Mahmoud Massoud Publisher: Springer (2005). Introduction To Thermal Systems Engineering: Thermodynamics, Fluid Mechanics And Heat Transfer, by Michael J. Moran, Howard N. Shapiro, Bruce R. Munson, David P. DeWitt, John Wiley & Sons edition (2003).

8. Fundamentals of Fluid Mechanics by Bruce R. Munson, Theodore H. Okiishi, Donald F. Young, John Wiley & Sons Inc, (2005). 9. Fluid Mechanics with Engineering Applications by E. John Finnemore, Joseph B. Franzini, McGraw-Hill College (2001). 10. Introduction to Thermal Systems Engineering:Thermodynamics, Fluid Mechanics, and Heat Transfer by Michael J. Moran, Bruce R. Munson, Howard N. Shapiro, David P. Dewitt , John Wiley & Sons Inc, (2002).

PROBABILITY AND STATISTICS Subject code : BTT.3.103 Sessional Examination Exam Duration : 30 : 70 : 3 Hrs

Periods / Week : 4 Nature of Examination : Theory

UNIT-1 PROBABILITY AND PROBABILITY DISTRIBUTIONS Probability: Sample space and events - counting - probability - The Awicens of probability some elementary theorems - conditional probability - mathematical; Probability Distributions: Random variables - The Binomial Distribution- The mean and the Variance of a probability Distribution - Chebyshev's theorem - The Poisson approves - motion of the binomial distribution - Poisson procession.

UNIT -II PROBABILITY DENSITIES AND SAMPLING DISTRIBUTIONS Probability Densities: Continuous Random Variables - The normal Distribution - The normal Approximation to the Binomial Distribution. Sampling Distributions: Populations and Samples; The sampling Distribution of the Mean (known) - The sampling distribution of the Mean (unknown) - The sampling Distribution of the Variance.

UNIT -III INTERFERENCE Interference Concerning Means: Point Estimation - Interval Estimation - Bayesians Estimation Tests of Hypothesis and Significant Tests - Hypothesis concerning one me

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