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b.tech Biotech II Yr i Sem_0

Dec 16, 2015

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Syllabus of Bio-Technology

BIOTECHNOLOGY SYLLABUSSCHEME OF INSTRUCTION AND EXAMINATION

B.Tech. (Biotech) II YEAR - I Semester

SUBJECT CODESUBJECTS

PERIODS / WEEK

Th/ LabMARKS

DURATION OF EXAM.

SessionalExamsHours

BTT.2.101

Mathematics - 1430703

BTT.2.102

Biochemistry430703

BTT.2.103

Cell Biology & Immunology430703

BTT.2.104

Process Engineering Principles430703

BTT.2.105

Chemical & Biochemical Thermodynamics430703

BTT.2.106

Environmental Studies430703

BTP.2.107

Biochemistry Lab425504

BTP.2.108

Cell Biology and Immunology Lab

425504

BTP.2.109

Multimedia Assisted Language Lab425504

255570

MATHEMATICS-I

Subject code : BTT.2.101 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : TheoryExam Duration : 3 Hrs

UNIT - I. DIFFERENTIAL EQUATIONS:

Formation of Differential Equations; Solution of First Order and First Degree Ordinary Differential Equations (Variable Separable, Homogeneous and Exact Differential Equations) -Equations Reducible to Homogeneous and Exact Differential equations; Linear Differential Equations; Integral Factors; Equations Reducible to Linear Equations; Linear Differential Equations of n* Order with Constant coefficients; Complementary Functions and Particular Integrals.

UNIT - II. LINEAR EQUATIONS OF SECOND ORDER:

i. Change of dependent variableii. Reduction to the normal fromiii. Change of independent variableiv. Variation of parameters

UNIT - III. POWER SERIES SOLUTIONS:Series Solution of Differential Equations: Ordinary and Regular points of a differential equation, series solution at an ordinary point and at a regular point.

UNIT - IV. SPECIAL FUNCTIONS:Properties of Gamma and Beta Functions.; Relation between Beta and Gamma Functions related Problems; Error Functions; Solution of Hyper geometric equation. Properties of hyper geometric function.

UNIT - V. SOLUTION SPECIAL EQUATIONS:Solution of Legendre's equation in series. Rodrigue's formula. Generating function, Recurrence formulae and Octagonal property. Solution of Bessel equation. Generating function. Recurrence formulae. Orthogonal property and Integral formula for Jn ( x)

EXAMINATION : One question from each unit with internal choice.

TEXT BOOKS:

1. Engineering Mathematics, B.V.Ramana, Tata McGraw-Hill (2003).

2. A text Book of Engineering Mathematics by P. Nageshwara Rao, Y. Narasimhulu & N. Prabhakar Rao, Deepthi Publications.

3. A Text Book of Engineering Mathematics, Vol-1 T. K. V. Iyengar, B. Krishna Gandhi and Others, S. Chand & Company.

REFERENCE BOOKS:1. Ordinary and partial differential equations by M.D. Raisinghania Sultan & Chand Co. (1991).2. Higher engineering mathematics by DR. B.S. Grewal, Khanna publishers(1998).

BIOCHEMISTRY

Subject code : BTT.2.102 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : TheoryExam Duration : 3 Hrs

UNIT-I: METABOLISM OF CARBOHYDRATES AND LIPIDSCarbohydrate Metabolism- Glycolysis, glycogenolysis, HMP, Citric Acid Cycle (TCA Cycle) and Oxidative Phosphorylation, Metabolic Pathways- Biosynthesis of Glucose, Glycogen and starch, Lipid Metabolism- Catabolism of Fatty Acids, Triglycerol and Cholesterol Metabolism; Metabolic Pathways- Biosynthesis of Saturated (stearic acid) and Unsaturated (Oleic acid) Fatty Acids, phospholipids and sphingolipids, Glycoproteins and Glyco Lipids.UNIT-II: METABOLISM OF PROTEINS AND NUCLEIC ACIDSAmino acids metabolism- Biosynthesis of Amino Acids, Peptides; Catabolism of Carbon skeletons of Amino Acids- Transamination, Oxidative Deamination and Oxidative Decarboxylation, metabolic fate of amino acids; Nitrogen Excretion and Urea Cycle;

Nucleic Acid Metabolism- Biosynthesis of Purine and Pyrimidine Ribonucleotides, synthesis of Deoxyribonucleiotides; Degradation of Purine and Pyramidine Nucleotides.

UNIT-III: BIOCHEMICAL PATHWAY OF PHOTOSYNTHESISPlant photosynthesis - Photosynthetic pigments and photochemistry; Hill reaction; Photosynthetic reaction centers- Photosystem -I, Photosystem II; Chloroplast- Organization of chloroplasts, chlorophylls trap solar energy; Oxygenic Photosynthesis - I; Photophosphorylation-Cyclic and Non-cyclic Photo Phosphorylation; Dark reaction - Carbon dioxide fixation in Ca path way (Calvin cycle).UNIT-IV: ENZYMES, COENZYMES AND METAL & NON METAL IONSEnzyme: Nomenclature, Classification, Properties and Functions; Coenzymes-Coenzymes in hydrogen transfer reactions (NAD+, FAD, Lipoic acid) and group transfer reactions(Biotin, TPP, Pyridoxal phosphate, Coenzyme A, Tetrahydrofolic acid), Metal ions Biological Systems - Role of Sodium, Potassium, Iron, Copper and Magnesium; Calcium, Phosphorus, Iodine and Sulfur.UNIT-V: HORMONES AND VITAMINSHormones- Classification of hormones-Based on the Chemical Nature & Mechanism of Action; Steroid hormones-Ovarian, Testicular hormones; Peptide hormones- Hormones of the Pancreas; Amino acid derivatives-Thyroidal and Parathyroid hormones; Vitamins - Classification (Water and Fat Soluble); Chemical Nature and Mechanism of Action of Vitamins; Biological Importance of Vitamins.

EXAMINATION: One question from each unit with internal choice

TEXTS BOOKS:

1. Lehninger Principles of Biochemistry: A.L, Nelson O.L, M.M. Cox, , CBS

Publications, 1993.

2. Biochemistry by Jeremy Berg (Author), John L. Tymoczko (Author), Lubert Stryer . W.H. Freeman & Company, (2006).

REFERENCE BOOKS:

1. Biochemistry - Voet D. and Voet J.G., 2nd edition, 1995, John Wiley publications, New York.2. Outlines of Biochemistry Conn and Stumpf, John Wiley, (2007)

3. Nelson, David L., Cox, Michael M. Lehninger Principles of Biochemistry 4/e, (2005), W.H. Freeman, Madison Avenue, New York.

4. Berg Jeremy M., Tymoczko, John L. , Stryer, Lubert Biochemistry 5/e, (2002), W.H. Freeman, New York.

5. Experimental Biochemistry by Switzer, Robert L., Garrity, Liam F. 3/e, (1999), W.H. Freeman, New York.

6. Biochemistry by Campbell K.M and Farrel O.S 5ed (2005), Thomson brooks and Cole.

7. Biochemistry, Berg M.J, Tymoczko J.L, Stryer L., 5ed ((2002)), W.H. Freeman, New York.

8. Harpers Biochemistry by Murray R.K, Granner D.K, Mayes P.A and Rodwell V.W 26ed (2003) Mcgraw Hill, New Delhi.

CELL BIOLOGY AND IMMUNOLOGY

Subject code : BTT.2.103 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : TheoryExam Duration : 3 Hrs

UNIT-I: CELL STRUCTURE, ORGANELLES AND THEIR FUNCTIONSCell structure and organization in Bacteria, Yeast, higher plants and animal cells. Structure and functions of cell wall, plasma membrane, lysosomes, Ribosomes, Golgi complex, Peroxisomes, Glyoxysomes, Mitochondria, Plastids, Endoplasmic Reticulum, Vacuoles, Centrioles, Cytoskeleton - composition, structure & functions of Microtubules, Microfilaments and Intermediate filaments.UNIT-II: NUCLEUS & CHROMOSOMESNucleus, its ultra structure, (Nuclear envelope, Nucleoplasm, Chromatin fibers); Structure of Nucleosome, Euchromatin, Heterochromatin, fine structure of chromosome, role of Histone & Non-Histone proteins in structural organization of Chromosomes, Giant chromosomes; Polytene & Lamp-brush chromosomes.UNIT-III: CELL DIVISION AND CELL CYCLECell Division: Mitosis - General events of Interphase, Prophase, Meta phase and Telophase; Meiosis - Heterotypic and Homotypic Meiotic division; Cytokinesis: Significance of Mitosis, Meiosis and Reproductive cycle.Cell cycle: Different phases of cell cycle, molecular basis of cell cycle regulation, cyclin dependent kinases, CDK- cyclin activity- Progress through cell cycle- Apoptosis.UNIT-IV: IMMUNE SYSTEMHistory of immunology, Structure, composition and functions of cells and organs involved in immune system- B-cells, T-cells, lymphocytes, phagocytes, auxiliary cells, soluble mediators -lymphokines, cytokines, interleukines; lymphoid organs- primary (thymus, bone marrow, bursa of fabricus) secondary (lymph node, spleen, MALT, GALT); Immune responses- innate and acquired immunity; humoral and cell-mediated immunity;UNIT-V: ANTIGENS, ANTIBODIES AND REACTIONSAntigens- nature and properties; haptens toxoids and vaccines; Immunoglobullins- structure, heterogenecity, types, antibody production - hybridoma technique, Antigen - antibody reactions-agglutination, precipitation, complement fixation; and Atomic Absorption microscopy for detection of antigen - antibody complex, ELISA, RIA.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. The Cell: A Molecular Approach by Geoffrey M. Cooper, Robert E. Hausman Publisher: Sinauer Associates; 3rd edition (2003).

2. Cell and Molecular Biology: Concepts and Experiments by Gerald Karp Wiley; 4th edition (2004).

3. Cell Biology CB Powar & SC Rastogi, Himalaya Publisher, (2006).4. Immunology Kuby, Richard Goldsby Thomas J Kinott Barbara and Osborne, W.H Freeman & Co. 5th edition, (2003).

REFERENCE BOOKS:1. Molecular Cell Biology - Damell J, Lodish H, Baltimore D, W H Freeman, (1990).

2. Essentials of Immunology- Riovett. Blackwell Scietific Publication Oxford 1991.

3. The world of cell Becker Reece Poenie 3rd edition Benjamin publishers

4. Immunology, Kindt Thomas J. ,Osborne, Barbara A., Goldsby, Richard A. Kuby 6/e, (2006), W.H.Freeman, Madison avenue, New York.

5. Immunobiology, by Charles A. Janeway, 6th edition, Garland Science Publishers.

6. Cellular and Mole

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