Biotechnology Questions

Biotechnology Questions, Paper - 01  

Questions Answers

1. The term cistorn, muton and recon were introduced by(A) Watson and Crick(B) S. Benzer(C) Meselson(D) Morgan

Answer: (B)

2. Extranuclear genetic material is found in (A) Plastid and nucleus(B) Mitochondria and plastids(C) Nucleus and cytoplasm(D) Mitochondria and nucleus

Answer: (B)

3. The molecular formulae of deoxyribose sugar and ribose sugar respectively are(A) C5 H10 O4 and C5H10O6 (B) C5 H10 O4 and C5H10O5 (C) C5 H10 O5 and C5H10O4 (D) C5 H10 O5 and C6H10O4

Answer: (B)

4. The nitrogen bases which pair with two hydrogen bonds are(A) Adenine and thymine(B) Adenine and Cytosine(C) Cytosine and guanine(D) Cytosine and adenine

Answer: (A)

5. DNA differs from RNA in(A) Presence of deoxyribose sugar(B) Presence of thymine base(C) Property of replication(D) All the above

Answer: (D)

6. DNA molecules makes a complete turn after every(A) 20 Å(B) 34 Å(B) 3.4 Å(D) 10 base pairs

Answer: (D)

7. The distance between two successive nitrogenous base pairs is(A) 34 Å(B) 36 Å (C) 20 Å(D) 3.4 Å

Answer: (D)

8. In nucleoside, nitrogen base is attached to pentose sugar at(A) Carbon – 1 of pentose sugar(B) Carbon – 2  of pentose sugar(C) Carbon – 4 of pentose sugar(D) Carbon – 5 of pentose sugar

Answer: (A)

9. If the strand of DNA has 35 nucleotide how many phosphodiester bonds would exist(A) 34(B) 35(C) 24(D) 70

Answer: (A)

10. In eukaryotic DNA replication, lagging strand is formed by(A) RNA fragments(B) Okazaki fragments(C) DNA fragments  (D) Nucleotide fragments

Answer: (B)

11. The enzyme DNA polymerase can work only in(A) 3  5 direction(B) 5  3 direction(C) Both the direction(D) 5  5 direction

Answer: (B)

12. Enzyme required for removing RNA primer during DNA replication is(A) DNA primase (B) DNA ligase(C) DNA polymerase I(D) DNA polymerase III

Answer: (C)

13. During DNA replication, the reunion or recoiling of separated DNA strand is prevented by(A) Helix destabilizing protein(B) Single strnad binding protein(C) Rep protein(D) Both (A) and (B)

Answer: (D)

14. The enzyme that cuts the bonds of DNA molecule at the origin of replication is(A) Endonuclease(B) DNA polymerase(C) DNA gyrase(D) DNA ligase

Answer: (A)

15. Which of the following enzyme is required to release the tension imposed by uncoiling of strands?(A) Endonuclease(B) DNA ligase(C) DNA gyrase(D) DNA helicase

Answer: (C)

16. The cellular composition of m-RNA is(A) 5-10%(B) 3-5%(C) 10-20 %(D) 70-80%

Answer: (B)

17. Formation of mRNA from DNA is called(A) Transformation(B) Transduction(C) Traslation(D) Transcription

Answer: (D)

18. The ratio of purines and pyrimidines in mRNA is not 1:1 because the nitrogenous bases are(A) Unpaired(B) Paired(C) Paired only in loops(D) Paired in stems

Answer: (A)

19. The codons which may present at 3¢ end of mRNA(A) UAA(B) UAG(C) UGA(D) Any one of these

Answer: (D)

20. Which of the following is not tool of genetic engineering?(A) Vectors(B) Enzymes(C) Foreign DNA(D) GMO

Answer: (D)

21. In recombinant DNA technology a plasmid vector is cleaved by(A) Modified DNA ligase(B) A heated alkaline solution(C) The same enzyme that cleave the donor DNA

Answer: (C)

(D) The different enzyme other than that cleave the donor DNA

22. The most common plasmid vector used in genetic engineering is(A) PBR 328(B) PBR 322(C) PBR 325(D) PBR 330

Answer: (B)

23. ‘Nif gene’ for nitrogen fixation is cereal crops like wheat, jowar etc. is introduced by cloning(A) Rhizobium meliloti(B) Bacillus thuringiensis(C) Rhizopus(D) Rhizophora

Answer: (A)

24. Eco RI is an(A) Ligase(B) Polymerase(C) Restriction enzyme(D) Gyrase

Answer: (C)

25. The transgenic plant flavr savr tomato carries an artificial gene for(A) Delay ripening process(B) Longer shell life(C) Added flavours(D) All of these

Answer: (D)

Biotechnology Questions, Paper - 02  

Questions Answers

1. Hirudin is obtained from the transgenic plant(A) Brassica napus(B) Hibiscus rosasinesis(C) Raphanus sativus(D) Vinca rosea

Answer: (A)

2. Bt Cotton is(A) Cloned plant(B) Transgenic plant(C) Hybrid plant(D) Mutated plant

Answer: (B)

3. Dolly sheep was genetically similar to (A) The mother from which nucleated fertilized egg was taken(B) The mother from which nuclear DNA of udder cell was taken(C) The surrogate mother(D) Both surrogate mother and nuclear donor mother

Answer: (B)

4. Genome is(A) Genes on nuclear DNA(B) Nuclear DNA + mitochondrial DNA(C) Nuclear DNA + chloroplast DNA(D) Nuclear DNA + Mitochondrial DNA + Chloroplast DNA

Answer: (D)

5. A technique of using very small metal particles coated with desired gene in the gene transfer is called(A) Electroporation(B) Microinjection(C) Liposome(D) Biolistics

Answer: (D)

6. The complete set of chromosomal and extrachromosomal genes of an organisms is called(A) Genome(B) Gene pool(C) Gene bank(D) Gene library

Answer: (A)

7. The study of all the proteins coded by the genome is called(A) Proteome(B) Proteomics(C) Genome(D) Protein formation

Answer: (B)

8. Sequencing of genomic DNA is included under(A) Structural genomics(B) Functional genomics(C) Proteomics(D) Transgenesis

Answer: (A)

9. Gene expression, regulation and phenotype production are studied in second phase of genome analysis called(A) Structural genomics(B) Functional genomics(C) Proteomics(D) Transmeiosis

Answer: (B)

10. A flowering plant lily have ______ more DNA than humans(A) 10 times(B) 15 times(C) 18 times(D) 13 times

Answer: (C)

11. In forensic science which of the following is used?(A) Bacterial cloning(B) DNA foot printing(C) DNA fingerprinting(D) DNA cloning

Answer: (C)

12. DNA fingerprinting is based on(A) Occurance of VNTR’s (B) Knowledge of human karyotype(C) Cloned DNA(D) Recombinant DNA

Answer: (A)

13. VNTRs represnets-(A) New terminal regions in DNA(B) Functional genes in the DNA(C) Split genes in the sample DNA(D) Specific non-coding sequences with unique tandem repeats

Answer: (D)

14. Which ones produce androgenic haploids in anther cultures?(A) Anther wall(B) Tapetal layer of anther wall(C) Connective tissue(D) Young pollengrains

Answer: (D)

15. Variations observed during tissue culture of some plants are known as(A) Clonal variations(B) Somatic variations(C) Somaclonal variations(D) Tissue culture variations

Answer: (C)

16. Virus free plants can be obtained through(A) Anitibiotic treatment(B) Bordeaux micture

Answer: (D)

(C) Root tip culture(D) Shoot tip culture

17. To raising of plants from a small tissue in culture is known as(A) Macroproduction(B) Micropropagation(C) Tissue culture(D) Mass production

Answer: (B)

18. Callus is(A) Tissue that forms embryo(B)  an insoluble carbohydrate(C) Unorganised actively dividing mass of cells maintained in culture(D) Tissue that growth to form embryoid

Answer: (C)

19. Biopatents are ______.(A) Right to use invention(B) Right to use biological entities(C) Right to use products(D) Right to use process

Answer: (B)

20. African plant Pentadiplandra is used as______.(A) Low calories sweetner(B) 2000 times sweeter agent(C) Sweetner for diabetic patients(D) All of these

Answer: (D)

21. Which organism was used as bioweapon derived from______.(A) Clostridium(B) Yerstsinia pestis(C) Fusarium species(D) Green algae

Answer: (C)

22. A set standards used to regulate own or community activity in relation to biological world is(A) Biopotency(B) Biopiracy(C) Biowar(D) Bioethics

Answer: (D)

23. Biopiracy means(A) Use of biopatents(B) Thefts of plants and animals(C) Stealing of bioresources(D) Exploitation of bioresources without authentic permission

Answer: (D)

24. Bioethcs is related to(A) Preventing biopiracy(B) Regulation of unethical activities likegene cloning in animals(C) Preventing theft of living materials(D) Moral guidance to the problems in biology

Answer: (B)

25. Three dimensional shape of tRNA is(A) L-shaped(B) Clover leaf-like(C) X-shaped(D) Y-shaped

Answer: (B)

Biotechnology Questions, Paper - 03

Sr. No. Questions Answers

1. A DNA nucleotide chain has AGCTTCGA sequence of other chain would be(a) TCGAAGCT(b) GCTAAGCT(c) TAGCATAT(d) GATCCTAG

Answer: (a)

2. A nucleoside is formed of (a) Pentose sugar, phosphate and nitrogen base(b) phosphate and nitrogen base(c) Pentose sugar and phosphate(d) Pentose sugar and nitrogen base

Answer: (d)

3. A nucleotide is formed of (a) Purine, Pyrimidine and phosphate(b) Purine, Sugar and phosphate(c) Nitrogen base, Sugar and phosphate(d) Pyrimidine, Sugar and phosphate

Answer: (c)

4. A riboside is(a) Base + phosphate(b) Ribose + phosphate(c) Ribose + phosphate + base(d) Ribose + base

Answer: (d)

5. A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is(a) 120(b) 240(c) 60(d) 480

Answer: (d)

6. A Strand of DNA has base sequence CATGACTAG. The base sequence on the other strand would be(a) CAT TAG GAC(b) GTA CTG ATC(c) GAT GTC ATC(d) TAC ACT GCT

Answer: (b)

7. A totipotent cell means(a) An undifferentiated cell capable of developing into a system or entire plant(b) An undifferentiated cell capable of developing into an organ(c) An undifferentiated cell capable of developing into complete embryo(d) Cell which lacks the  capability differentiate into an organ or system

Answer: (a)

8. Adenine is(a) Purine(b) Pyrimidine(c) Nucleoside(d) Nucleotide

Answer: (a)

9. Amino acid binding site of tRNA is(a) 5’end(b) Anticodon loop(c) DHU loop(d) -CCA 3’end

Answer: (d)

10. Anticodon occurs in(a) tRNA(b) mRNA(c) mtRNA

Answer: (a)

(d) rRNA

11. Bacterial plasmid contains(a) RNA(b) RNA + protein(c) DNA(d) Photosynthetic structures

Answer: (c)

12. Base pairs present in one turn of DNA are (a) 12(b) 11(c) 10(d) 9

Answer: (c)

13. Best method to determine paternity is (a) Protein analysis(b) Chromosome counting(c) Gene counting(d) DNA finger printing

Answer: (d)

14. Callus is (a) Tissue that forms embryo (b) An insoluble carbohdrate(c) Tissue that grows to form embryoid(d) Unorganised actively dividing mass of cells maintained in culture

Answer: (d)

15. Chemical Knives/ molecular scissors of DNA are(a) Restriction endonucleases(b) Polymerases(c) Ligases(d)Transcriptases 

Answer: (a)

16. Chemofusion and electrofusion are employed in(a) Eugenics(b) Protoplast fusion(c) Cloning(d) Mutations

Answer: (b)

17. Choose the correct statement (a) DNA is hereditary material(b) RNA is hereditary material(c) DNA is hereditary material but where it is absent RNA can function as hereditary material(d) Both DNA and RNA are hereditary materials

Answer: (c)

18. Development of shoot and root in tissue culture is determined by(a) Cytokinin and auxin ratio(b) Enzymes(c) Temperature(d) Plant nutrients

Answer: (a)

19. Distance between two base pairs of DNA is(a) 34 nm(b) 3.4 nm(c) 0.68 nm(d) 0.34 nm

Answer: (d)

20. Distance between two strands of DNA is(a) 34 Å (b) 20 Å (c) 3.4 Å (d) 340 Å 

Answer: (b)

Biotechnology Questions, Paper - 04

Sr. No. Questions Answers

1. Distance between two successive nitrogenous bases or base pairs of DNA is (a) 34 Å (b) 3.4 Å (c) 10 Å (d) 5 Å 

Answer: (b)

2. DNA and RNA are similar in having(a) Similar nucleotides(b) Similar pyrimidines(c) Similar purines(d) Similar sugars

Answer: (c)

3. DNA and RNA show similarity in having(a) Polymers of nucleotides(b) Similar pyrimidines(c) Double strands(d) Similar sugars

Answer: (a)

4. DNA does not occur in(a) Nucleus (b) Ribosomes(c) Mitochondria(d) Plastids

Answer: (b)

5. DNA duplex shows (a) Right handed coiling and parallel(b) Right handed coiling and antiparallel(c) Left handed coiling and antiparallel(d) Left handed coiling and parallel

Answer: (b)

6. DNA is composed of repeating units of(a) Ribonucleosides(b) Deoxyribonucleosides(c) Ribonucleotides(d) Deoxyribonucleotides

Answer: (d)

7. DNA replication in eucaryotes commences(a) From both ends of a chromosome simultaneously (b) Several sites along DNA of a chromosome simultaneously(c) From centromere to either end(d) From one end of chromosome to the other

Answer: (b)

8. DNA replication is(a) Conservative and discontinuous(b) Semiconservative and semidiscontinuous(c) Semiconservative and discontinuous(d) Conservative

Answer: (c)

9. DNA resembles RNA as both have(a) Polymers of nucleotides(b) Similar sugars(c) Similar pyrimidine bases(d) Ability to replicate

Answer: (a)

10. DNA sequence is ATG. What would be the sequence of bases in anticodon of tRNA(a) ATG

Answer: (b)

(b) AUG(c) UAC(d) TAC

11. DNA sequence is TAG. What shall be the sequence in anticodon of tRNA(a) UAG(b) ATC(c) ATG(d) UAC

Answer: (a)

12. DNA sequence of ATTCGATG is transcribed as(a) AUUCGAUG(b) UAAGCUAC(c) CAUCGAAU(d) GUAGCUUA

Answer: (b)

13. DNA strand with nitrogen base sequence ATTGCC will have sequence in mRNA(a) UAACGC(b) ATCGCC(c) ATTGCA(d) AGGACC

Answer: (a)

14. Double chained DNA strand is made radioactive in both its chains. It is allowed to replicate twice in non-radioactive medium. The result would be(a) All strands have radioactivity(b) Half the strands have radioactivity(c) Three strands have radioactivity(d) Radioactivity is absent in all strands

Answer: (b)

15. Double helical structure of DNA was proposed by(a) Kornberg(b) Nirenberg(c) Watson and Crick(d) Wilkins and Franklin

Answer: (c)

16. Functional unit of gene that specifies synthesis of one polypeptide is(a) Codon(b) Cistron(c) Recon(d) Muton

Answer: (b)

17. Gene is segment of (a) RNA(b) DNA(c) RNA or DNA(d) Both DNA and RNA

Answer: (b)

18. Genetically engineered bacteria are being used in commercial production of(a) Melatonin(b) Testoteron (c) Human insuline(d) Thyroxine

Answer: (c)

19. Haploid plant cultures are got from(a) Leaves(b) Root tip(c) Pollen grain(d) Buds

Answer: (c)

20. Hydrogen bonds present between cytosine and guanosine are(a) 2(b) 3(c) 1

Answer: (b)

(d) 4

Biotechnology Questions, Paper - 05

Sr. No. Questions Answers

1. In a DNA molecule cytosine is 18%. Percentage of adenine would be(a) 32%(b) 64%(c) 36%(d) 18%

Answer: (a)

2. In AGCT of DNA hydrogen bonds and base pairings occur between(a) A-U, C-G(b) A-C, G-T(c) A-G,C-T(d) A-T,C-G

Answer: (d)

3. In callus culture, roots can be induced by the supply of (a) Auxin and no cytokinin(b) Higher concentration of auxin and lower concentration of cytokinin(c) Higher concentration of cytokinin and lower concentration of auxin(d) Both auxin and cytokinin in equal proportions.

Answer: (b)

4. In DNA, adenine pairs with(a) Guanine(b) Thymine(c) Cytosine(d) Uracil

Answer: (b)

5. In DNA, guanine lies opposite(a) Uracil(b) Cytosine(c) Adenine(d) thymine

Answer: (b)

6. In double helix of DNA, the two DNA strands are(a) Coiled around a common axis(b) coiled around each other(c) coiled differently(d) Colied over protein sheath

Answer: (a)

7. In RNA, thymine is replaced by(a) Adenine(b) Guanine(c) Cytosine(d) Uracil

Answer: (d)

8. In tissue / bacterial culture glassware and nutrients are sterilized through(a) Water bath at 200° C(b) Dry air oven at 200° C(c) Dehumidifire(d) Autoclave

Answer: (d)

9. In tissue culture, callus can be induced to form shoot or root by altering the ratio of(a) Auxin to cytokinin(b) Cytokinin to ethylene

Answer: (a)

(c)  Auxin to gibberellin(d) Gibberellin to cytokinin

10. Initiation codon of protein synthesis (in eucaryotes) is(a) GUA(b) GCA(c) CCA(d) AUG

Answer: (d)

11. Introduction of foreign genes for improving genotype is(a) Tissue culture(b) Immunisation(c) Biotechnology(d) Genetic engineering

Answer: (b)

12. It is now possible to breed plants and animals with desired characters through(a) Genetic engineering(b) Chromosome engineering(c) Ikebana technique(d) Bonsia technique

Answer: (a)

13. Most abundant RNA of the cell of(a) tRNA(b) rRNA(c) mRNA(d) tRNA

Answer: (b)

14. Nitrogen bases of DNA are (a) ATUC(b) UTGC(c) ATGC(d) AUGC

Answer: (c)

15. Nonsense codon takes part in(a) Terminating message of gene controlled protein synthesis(b) Formation of unspecified amino acids(c) Conversion of sense DNA into non-sense one(d) Releasing tRNA from polypeptide chain

Answer: (a)

16. Nucleosides are(a) Sugar + Phosphoric acid(b) Purine / Pyrimidine + Sugar + Phosphate(c) Purine / Pyrimidine + Phosphoric acid(d) Purine / Pyrimidine + Sugar

Answer: (d)

17. Nucleotides present in one turn of DNA helix (a) 4(b) 8(c) 10(d) 9

Answer: (c)

18. Okazaki segments are formed during(a) Transduction(b) Transcription(c) Replication(d) Translation

Answer: (c)

19. Plants developed in vitro culture from pollen grains are(a) Androgenic plants(b) Pollen plants(c) Male plants(d) Sterile plants

Answer: (a)

20. Plasmids are vectors for gene cloning because they Answer: (a)

(a) Self replicate in bacterial cells(b) Replicate freely outside bacterial cells(c) Can be multiplied in culture(d) Can be multiplied in laboratories using enzymes

Biotechnology Questions, Paper - 06

Sr. No. Questions Answers

1. Pomato is somatic(a) Poppy and Potato(b) Potato and tomato(c) Poppy and tamarind(d) Poppy and Tomato

Answer: (b)

2. Preserving germplasm in frozen state is(a) Cryopreservation(b) Cold storage(c) In situ preservation(d) Vernalisation

Answer: (a)

3. Protein helping in opening of  DNA double helix in front of replication fork is(a) DNA gyrase(b) DNA Polymerase I(c) DNA ligase(d) DNA topoisomerase

Answer: (a)

4. Pyrimidine base present in RNA in place of thymine of DNA is(a) Uracil(b) Adenine(c) Cytosine(d) Guanine

Answer: (a)

5. Restriction endonuclease is employed for cutting(a) A single stranded DNA(b) Double stranded DNA(c) RNA fragment(d) mRNA

Answer: (b)

6. Restriction endonucleases are(a) Used in genetic engineering for uniting two DNA molecules(b) Used for in vitro DNA synthesis(c) Present in mammalian cells for degeneration of DNA of dead cells(d) Synthesised by bacteria for their defence

Answer: (d)

7. Restriction enzymes are used in genetic engineering because they(a) Can join DNA fragment (b) Cut DNA at specific base sequence(c) Cut DNA at variable sites(d) Are proteolytic enzymes which degrade harmful proteins

Answer: (d)

8. Reverse transcriptase is(a) RNA dependent RNA polymerase

Answer: (d)

(b) DNA dependent RNA polymerase(c) DNA dependent DNA polymerase(d) RNA dependent DNA polymerase

9. RNA contains(a) Hexose(b) Ribose(c) Fructose(d) Glucose

Answer: (b)

10. RNA does not possess(a) Uracil(b) Thymine(c) Adenine(d) Cytosine

Answer: (b)

11. RNA that picks up specific amino acid from amino acid pool of cytoplasm to carry it to ribosome during protein synthesis is(a) tRNA(b) mRNA(c) rRNA(d) gRNA

Answer: (a)

12. Semiconservative DNA / Chromosome replication using 14N was demonstrated by(a) Messelson(b) Tylor(c) Messelson and stahl(d) Hershey and Chase

Answer: (c)

13. Similarity between DNA and RNA is that both have(a) Similar sugars(b) Similar mode of replication(c) Similar pyrimidines(d) Polymers of nucleotides

Answer: (d)

14. Structure of DNA was given by(a) Kornberg(b) Nirenberg(c) Watson and Crick(d) Holley and Nirenberg

Answer: (c)

15. Successive nucleotides are covalently linked through(a) Glycosidic bonds(b) Phosphodiester bonds(c) Hydrogen bonds(d) Nitrogen bonds

Answer: (b)

16. Termination of polypeptide chain is brought about by(a) UUG, UAG and UCG(b) UAA, UAG and UGA(c) UUG, UGC and UCA(d) UCG, GCG and ACC

Answer: (b)

17. The basic uniot of a nucleic acid is(a) Pentose sugar(b) Nucleoid(c) Nucleoside(d) Nucleotide

Answer: (d)

18. The codon for anticodon 3’ UUUA - 5’ is(a) 5’ AAAU- 3’(b) 5’ UUUA- 3’(c) 3’ UAAD- 5’

Answer: (a)

(d) 3’ AUUU- 5’

19. The common feature amongst nucleus, chloroplast and mitochondria is(a) Lamellae(b) DNA(c) Cristae(d) All the above

Answer: (b)

20. The enzyme taking part in joining two ends of DNA is(a) Ligase(b) Polymerase(c) Gyrase(d) Helicase

Answer: (a)

CHAPTER – 9

1. Genetic engineering is the manipulation of ________ for practical purpose.

Genetic bacteria

Genetic plant

Genetic material

Genetic animal

2. The ________ technology has launched an industrial revolution in bio-technology.

DNA transfer

DNA recombinant

genetic bacteria

genetic transferring

3. Recent reproductive cloning intended to introduce ________.

identical plants

genetic bacteria

identical animals

identical sheeps

4. ________ was the first ever cloned animal.

Dolly

Tomy

Kitty

Spike

5. ________ formulated rule to explain the phenomenon of inheritance.

Gregor Johann Mendel

Charles Darwin

Jean Baptiste de Lamark

All of them

6. Each basis inheritable character is controlled by a factor called ________.

Chromosome

DNA

RNA

gene

7. Gene is made up of ________.

Proteins

RNA

DNA

All of them

8. The discovery of ________ was a tremendous stimulus to genetic research.

DNA of bacteria

cloned Dolly

Role of DNA

genetic engineering

9. Methodology was discovered in ________.

1952

1956

1973

1989

10. Methodological methods were referd to as ________.

Recombinant DNA technology

Genetic engineering

Both

All of them

11. ________ is defined as the “se of living organisms for the welfare of mankind”.

Micro-biology

Human biology

Bbiotechnology

Zoology

12. Genetic engineering usually utilizes ________.

Bacterial cells

Bacterial plasmids

Both of them

None of them

13. Recombinant DNA technology depends on ________ steps.

3

4

5

8

14. A gene is inserted into a DNA molecule called ________.

vector

pasmid

both

none of them

15. ________ are small, extra circular DNAs molecules found in some bacteria.

vector

plasmid

chromosome

genetic engineered DNA

16. Plasmid replicated ________ of the host cell.

dependent

independent

mostly dependent

mostly independent

17. Plasmids are generally found in ________.

bacteria

vertebrates

all living organisms

bacteriophages

18. The group of enzymes that are used to cut up the DNA molecule are called ________.

restriction enzymes

DNA ligase enzyme

DNA polymerase enzyme

none of them

19. ________ protest bacteria against bacteriophage by cutting viral DNA.

DNA polymerase enzyme

restriction enzyme

DNA ligase enzyme

all of them

20. The molecular scissors in the bacterial cell are ________.

DNA ligase enzyme

vector

plasmid

restriction enzyme

21. Each restriction enzyme cut the DNA at a special site called ________.

sticky end

site of attachment

cutting end

joining ends

22. ________ is a key enzyme that seals the restriction fragment with sticky ends of vector.

DNA polymerase enzyme

DNA ligase enzyme

Restriction enzyme

A and B

23. ________ are taken as host in DNA recombinant technology.

bacterial cells

vectors

plasmids

bacteriophage

24. The gene carried by recombinant molecule is called ________.

cloned

copied

multiplied

engineered

25. ________ is used to form genetically engineered plants which are resistant towards insects.

genetically engineered bacteria

vector

plasmid

all of them

26. Bacteria which usually live in the roots of corn are transformed for producing ________.

good roots

strong barks

insect killing foxins

healthy fruits

27. Hormones can also be prepared by ________.

cloned bacteria

plasmid

vector

rDNA

28. Phynylalanine is used to make a sweetener called ________.

phenyl sweets

nutra sweets

cemilky sweets

natural sweets

29. When a foreign gene is inserted to natural plants they are called ________.

Transgenic plants

genetically engineered plants

both A and B

all of them

30. In ________ technique entire organism can be grown from a single call.

tissue culture

cloning

genetic engineering

transfusion

31. When the cell wall of a plant cell is removed it is called ________.

protoplast

plant cell

changed cell

transgenic cell

32. In plant cell the rDNA can be introduced into ________.

Enbryo

Protoplast

Cell having cell wall

Both A and B

33. The only plasmid for transgenic plants is ________.

Pr-plasmid

Ti-plasmid

Ds-plasmid

All of them

34. ________ is the host for the host for the Ti-plasmid.

Bacteriophage

Agrobacterium

Phenylalamine

All bacteria

35. Transgenic plants are able to make use of ________ of the atmosphere so they dont require fertilizers.

Oxygen

Nitrogen

CO2

All of them

36. ________ is the fastest way of determining amino acid sequences.

DNA finger printing

Gene sequencing

Genomic library

Human genome project

37. ________ provides the location of Restriction site within the gene.

Gene sequencing

DNA finger printing

Genomic library

All of them

38. The similarity in the DNAs of two different organisms can be determined and studied by ________.

DNA finger printing

Human genome project

Gene sequencing

Gene therapy

39. ________ can separate DNA fragments differing in as much as 1 segment in gene sequencing.

Gel electrophoreses

DNA electrophoreses

Gene electrophoreses

All of them

40. ________% of DNA does not code for proteins

20%

30%

40%

50%

41. The difference in DNA electrophoresis patterns among individuals is called ________.

DNA finger prints

DNA fragment length

DNA length measurement

Restriction fragment length polymorphism

42. DNA finger prints is named so due to ________

RFLPs length

Sequence of RFLPs is unique for each

Both A and B

None of the above

43. To produce a DNA finger print ________ would suffice.

A sample of blood

A sample of saliva

Sample of hair folicle

All of them

44. ________ consists of the copy of genetic information or a specre the prefered environment.

Genome library

DNA finger prints

DNA fragment

Gene sequencing

45. Human Genome Project began in ________.

1990

1980

1994

1991

46. Total number of chromosome pairs inhuman is ________.

23

26

44

46

47. ________% of the DNA sequence have determined with 99% accuracy.

89%

95.658%

99.335%

99.99%

48. ________% of the nucleotide sequence in all the hamans is same.

50%

86%

99%

99.9%

49. Medical researches have discovered about ________ genetic diseases.

3000

4000

5000

6000

50. Genetic diseases is mostly related with ________.

Sperms

Ova

Fertilized egg or zygote

All of them

51. There are ________ conditions for the occurance of genetic diseases.

2

3

5

9

52. Is mutation a cause of genetic diseases?

Yes always

No

May be

Not involved at all

53. Genetic diseases fall into ________ main categories.

2

3

5

8

54. Following is the category of genetic diseases.

Chromosomal abnormalities

Unifactorial defects

Multifactorial defects

All of them

55. Huntington’s disease is due to a ________.

Autosomal resesive allele

Autosomal dominant allele

Hetyerozygous allele

Homozyhous allele

56. In huntington’s disease the symptoms appear by the age of ________.

35-45

40-50

55-65

50-60

57. Progressive mental deterioration and unvoluntary muscle movement is the characteristic of ________.

Chromosomal disorder

Chromosomal disjunction

Huntington’s disease

Cystic fibrosis

58. Is a cure for huntington’s disease possible.

Yes

May be

Depends on nature

No

59. Cystic fibrosis occurs due to ________

Autosomal Recessive Allele

A heterotroph

Homozygous Chromosome

Dominant Allele

60. ________ is a diagnostic process in which man amount of amniotic fluid is taken from the amniotic sac to

determine the presence of disease.

Genetic councelling

Tissue culture

Gene therapy

Amniocentesis

61. Through ________ any disease can be determined in the embryonic stage.

Amniocentesis

Amniotic tissue test

fefune blood test

Gene therapy

62. ________ is a test tube method to create and olone various varieties of novel plants.

Cloning

Genetic engineering

Gene therapy

Tissue culture

63. The cell which contains all the genetic potential of the organism is called ________.

Potitotent

Totipotent

Sturgid call

Complete cell

64. Through cloning the duplicate coples of ________ can be formed.

Genetic material

Cells

Both of them

None of them

65. Artificial cloning is now possible by the pethology ________.

Recombinant DNA technology

Gene therapy

DNA finger prints

Genomic library

66. The copies of individuals formed by clonig are called ________.

Twins

Identicals

Clones

Copies

67. In 1997, a ________ was cloned from cell from its mother.

Cat

Dog

Fish

Sheep

68. The biotechnology is now also being considered dangerous due to

Its useful cloning method

DNA finger printing

Harmful side effects

Artificial Techniques

69. In gene therapy of germ line cells the recombinant DNA is inserted into ________.

Human sex cells

Fertilized zygote

Somatic cells

All of them