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1 2010 12 15 ( ) , ( , PWRI) Andrew JOHNSON (Centre for Ecology and Hydrology, CEH) 22
27

BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

Oct 17, 2019

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Page 1: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

1

2010 12 15

( )

, ( , PWRI)Andrew JOHNSON

(Centre for Ecology and Hydrology, CEH)

22

Page 2: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

2005 2009

• Roach

EDC

2

Page 3: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

0 per cent

1–20 per cent

21–40 per cent

41–60 per cent

61–80 per cent

81–100 per cent

Jobling et al., 2006)

3

Page 4: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

17�-estradiol (E2)

Estrone (E1)

OH

OH

O

OH

Ethynylestradiol EE2)

OH

OH

ng/L4

Page 5: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

0

2

4

6

8

10

12

14

16

18

England Japan

No

rma

lis

ed

ex

cre

tio

n E

2 e

qu

iv u

g/h

ea

d/d

2%( , 2001)

%

E2 (Johnson et al., 2006

0.6 %

?

? ? ?

Page 6: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

6

1)2)3)

UK-J Phase 2 CORE 1 (2005 to 2009)

Page 7: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

Estradiol (E2) Estriol (E3) Estrone(E1)

( or

Ethynyl estradiol (EE2)

O

C ONa

OH

HO

OH

O

OHSO

O

OH

OH

7

Page 8: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

Sample

SPE

(GF/B)

Oasis HLB

STD

NH2 Cartridge

CH3CN/H2O(1:9),1ml

MeOH 0.5% NH4OH/MeOH

N2 Evap.

LC-MS/MS LC-MS/MS

Clean up

Clean-up with NH2

Change LC 50 mm 100 mm

: 500 ml

/ : 1000 ml

8

Page 9: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

0

10

20

30

40

50

60

70

80

90

100

E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

Con

cent

ratio

n (n

g/L)

Influent Effluent

UK (n = 3)

� E1, E2 EE2

=Range

9

Page 10: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

� EE2

� E1� E1

n=29

0

10

20

30

40

50

60

70

80

90

100

E1 E2 E3 E1-3S E2-3S E3-3S E1-3G E2-3G

Con

cent

ratio

n (n

g/L)

0

10

20

30

40

50

60

70

80

90

100

Rem

oval

effi

cien

cy (

%)

Influent Effluent Removal

10

Page 11: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

0

20

40

60

80

100

E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

Rem

oval efficien

cy (%

)

Removal efficiency

-80

-60

-40

-20

0

20

40

60

80

100

E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

Rem

oval

effi

cien

cy (%

)E2,EE2

E1

Jap

an

(n

= 9

)U

K (

n=

3)

=Range

11

Page 12: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

12

� E1 EE2

E2

� EE2

0

10

20

30

40

50

Con

cent

ratio

n (n

g/L)

Influent

Effluent

Downstream

E1 E2 E3 EE2 E1-3S E2-3S E3-3S E2-3&17GE3-3&17G

Co

nc

en

tra

tio

n (n

g/L

)

Page 13: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

13

Benson Sandhurst

R. Thames Camberley

Parking

Sandhurst

Page 14: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

14

E2

?

PST Reactor FST

Qs

Qs Qs

PST Reactor FST

Qs

Qs

kQs

Qs

kQs

+

Page 15: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

SRT

15

SRT=22E1 E2

Page 16: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

�� ..

N=6

0

2

4

6

8

10

12

14

16

18

20

E1 E2 E3 E1-3S E2-3S E3-3S

Con

cent

ratio

n (n

g/L)

0

20

40

60

80

100

Rem

oval

effi

cien

cy (

%)

Secondary Effluent After Ozonation Removal

16

Page 17: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

D7

D4

D5

D3

D2

D9

D8D6

D1

U2

U1

K1

U9 U6

U7

U9

U10

K7

K9K8

K6

K5

K10

K4

K2

K3

U3 U4

U9

17

�E2 EE2 , E1

�E1 ,

�E1

Page 18: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

(segment)

(segment)

STP

STP

STP

60

, , ,

18

Page 19: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

0

1

2

3

4

5

6

7

8

9

10

Fro

me

Dar

t

Tor

ridge

Cuc

kmer

e

Gre

at S

tour

Aru

n

Soa

r

Tem

e

Cro

uch

Cam

Wea

r

Tee

s

Ede

n

Cal

der

Wyr

e

Taf

f

Tyw

i

Eas

tern

Cle

ddau

Con

wy

Mer

sey

Irwel

l

Aire

Don

Bly

th

Ere

was

h

Tre

nt

Idle

Littl

e O

use

Wav

eney

Tha

mes

Med

way

Avo

n

0

1

2

3

4

5

6

7

8

9

1075% 25%

19

EEQ (ng/L) E

sti

mate

d E

EQ

E2-e

qu

ivale

nt,

ng

/L

ng/L

Page 20: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

Risk Class

No risk

At risk

High risk

LF2000-WQX

(>1 ng/L EEQ)

(>10 ng/L EEQ)20

Page 21: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0R

umoi

Sar

u

Tok

oro

Ishi

kari

Aka

Yon

esiro

Kita

kam

i

Tsu

rum

i

Kuj

i

Nak

a

Kak

ehas

hi

Kur

obe

Ara

Shi

nano

Kik

u

Abe

Syo

unai

Kis

o

Kita

Yam

ato

Yur

a

Ash

ida

Asa

hi

Gou

no

Dok

i

Mon

obe

Niy

odo

Yos

hino

Hom

myo

Kim

otsu

ki

Ooi

ta

Chi

kugo

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

1ng/L

30.6 18.0

98Summer

98Autumn

99Spring

99Summer

99Autumn

99Winter

00Autumn

01Autumn

NA NA NA NA NA

NA:No available data

21

Estr

og

en

ic A

cti

vit

ies, E

2-e

qu

ivale

nt,

ng

/LEEQ

(ng/L)

YES Assayw/ hER

Page 22: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

22

Page 23: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

•• k=0.067 /h

0

1

2

3

4

0 1 2 3 4

Estimated Concentration (ng/L)

Mea

sure

d C

once

ntra

tion

(ng/

L)

Theoretical line (x=y)

23

Page 24: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

24

Page 25: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

� SRT E1 , O3.

25

SRT + O3

E1

25

Page 26: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

• EE2E2

• SRT

Summary

26

Page 27: BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

27

Acknowledgements

12th Annual Scientific Workshop UK-Japan Co-operation for Research on Endocrine Disrupters in the Aquatic Environment held at Otterburn HallNorthumberlandNational Park UK on 2-4 November 2010