Announcements This course is MCB 110L Prerequisites : BMB, Track 1: MCB C100A; MCB 100B; MCB 110 (may be taken concurrently) BMB, Track 2: MCB C100A; Chem. 135; MCB 110 (may be taken concurrently) Chem. Bio.: Chem. C130-130B (or Chem. 120A-120B); Chem. 135; MCB 110 (may be taken concurrently) Lab Manual : Purchase ASAP at Copy Central, 48 Shattuck Square Required Reading for Lecture #1 : Short article by Botstein & Fink Additional Required Reading : Each experimental protocol and the additional background articles by Sherman in the Lab Manual
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Announcements This course is MCB 110L Prerequisites: BMB, Track 1: MCB C100A; MCB 100B; MCB 110 (may be taken concurrently) BMB, Track 2: MCB C100A; Chem.
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AnnouncementsThis course is MCB 110L
Prerequisites:BMB, Track 1: MCB C100A; MCB 100B; MCB 110 (may be taken concurrently)BMB, Track 2: MCB C100A; Chem. 135; MCB 110 (may be taken concurrently)Chem. Bio.: Chem. C130-130B (or Chem. 120A-120B); Chem. 135;
MCB 110 (may be taken concurrently)
Lab Manual: Purchase ASAP at Copy Central, 48 Shattuck Square
Required Reading for Lecture #1: Short article by Botstein & Fink
Additional Required Reading: Each experimental protocol and theadditional background articles by Sherman in the Lab Manual
Exam Date: FRIDAY, 22 February 2008
Course Policies: READ THE HANDOUT (& remember its content)
Pre-lab Safety Lecture (~15 min): Ann Fischer BMB Laboratories Supervisor
For those of you who are already planning your post-Cal life, and arranging for career-related interviews (e.g., at medical schools, graduate schools, pharmacy schools, optometry schools, veterinary schools, pharmaceutical companies, biotech firms, etc. etc.), please keep in mind that:
(1) Each student enrolled in MCB 110L is responsible for all lecture material and lab experiments.
(2) If a lecture is missed or a lab is missed, there is no make-up for either.
(3) If an in-class pop quiz is missed, there is no make-up for the missed quiz.
(4) Missing a lecture or a lab also costs points in "lab management", which is 10% of your final grade.
(5) All experiments are conducted in teams of two; hence, your absence hurts your lab partner.
(6) The instructor may consider imposing an additional penalty for any unexcused absence from lecture or lab (such as completion of an additional relevant assignment outside of class). See: MCB 110L Policy Statement for definition and discussion of excused absences.
(7) I hope you find the above information helpful in assisting you with scheduling your commitments this Spring Semester.
GRADING:
Five lab reports at 100 pts. per report = 500Flowcharts for 5 exps. at 20 pts. per chart = 100Occasional in-class pop quizzes = 100General lab management and decorum = 100End-of-Section I Examination = 200
TOTAL 1,000
MCB 110L: Section I— The Power of Molecular GeneticsKeep your eyes on the prize: The objects of our focus for the next
five weeksGene Transcription by EukaryoticRNA Polymerase II
Initiation of mRNA Translationby Eukaryotic Ribosomes
Ribbon diagram of the structure of yeast RNA polymerase II at 2.8 Å resolution.Identity and location of each of the ten subunits is color-coded and interactionsbetween subunits (thickness of the connecting line represents the amount ofsurface area buried in the corresponding subunit interface) are depicted in theschematic diagram to the right. See: Cramer P, et al. (2001) Science 292: 1863-1876.