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    offensive and potentially dangerous substances which cause pollution and contamination of

    receiving ecosystems. Ghosh and Singh (2005) reported that controlled and uncontrolled disposal

    of waste, sewage sludge application to agricultural soils, accidental and process spillages are

    responsible for the migration of contaminants into the soil. Larson, (2003) reported that Physico-

    chemical disturbances are primarily responsible for influencing plant community composition

    and the spread of invasive exotics. The plant species found along diversely polluted effluent

    channels include highly resilient species that are resistant to high amount of different heavy

    metals (Li et al., 2004; Juknevicius et al., 2007; Al-Khashman, 2007; Yetimoglu et al., 2007).

    Plants colonizing metal-contaminated soils are classified as resistant and have adapted to this

    stressed environment. Heavy metal resistance can be achieved by avoidance and/or tolerance.

    Some plant species are able to protect themselves by preventing heavy metal ions from entering

    their cellular cytoplasm and are termed Avoiders.Others are able to detoxify metal ions that

    may have crossed the plasma membrane and are termed Tolerant species (Millaleo et al.,

    2010). A strategy explored by avoiders involves mycorrhizal fungi, where they can extend their

    hyphae outside the plant rooting zone up to several tens of meters and transfer the necessary

    elements to the plant (Ernst, 2006; Baker, 1987). Plants can also restrict contaminant uptake in

    root tissues by immobilizing metals, i.e. through root exudates in the rhizosphere. A series of

    root exudates is to chelate metals and stop their entry into the cell. Tolerant plants are protected

    internally from the stress of metals that gained entry into the cell cytoplasm (Baker, 1987).

    Metallophytes can function normally even when there is a higher plant- internal metal level

    by developing heritable tolerance mechanisms over time. Different species from local non

    tolerant ancestral plant populations have independently evolved mechanisms to tolerate specific

    metals (Schat et al., 2000). Plants exhibit tolerance to metals that are present in excess in soil,

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    with each metal under the control of a specific gene (genostasis). It is the restriction of this

    genetic variability that limits the evolution of the population/species. Metal tolerance and

    accumulation in plants are complex genetic systems. Plants have to modify their physiological

    processes in order to be able to survive in the environment in which they have germinated. In

    turn, the survival of a population to the contaminated environment is dependent on the

    inheritance of favourable traits. Bradshaw, (1991) reported that in the absence of avoidance

    pathways, soils contaminated with heavy metal act as a force on the plant population where

    plants with tolerant genotype can survive and reproduce. Tolerance mechanisms are heritable

    and variable, resulting from genes and gene products (Maestri and Marmiroli 2012). The gene

    for tolerance pre-exist at a low frequency in non tolerant populations of certain plant species

    (Ernst, 2006; Macnair, 1987).

    Baker and Walker, (1990) classified plants based on the strategies used by them as; metal

    excluders, indicators and accumulators/hyperaccumulators. The former limits the translocation of

    metals, maintaining low levels of contaminants in their aerial tissues over an extensive range of

    soil concentrations. The latter (indicators) accumulate metals in their harvestable biomass and

    these levels generally are reflective of the metal concentration in the soil. Metal

    accumulators/hyperaccumulators are plants that increase internal sequestration, translocation and

    accumulation of metals in their harvestable biomass to levels that far exceed those found in the

    soil (Mganga et al., 2011; Baker and Walker 1990).Resistance is a quantitative trait that

    enables a plant to survive, grow and reproduce in the presence of a particular contaminant (Baker

    and Walker, 1989). Plant populations can become resistant to heavy metals by genetic

    adaptation or gradual acclimatization to an increasing heavy metal load (Antonovics et al. 1971;

    Baker et al.,1986; Dickinson et al.,1991; Punshon and Dickinson 1997). All these strategies,

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    allow the establishment of plant communities on metal contaminated soils along an effluent

    channel. The accumulation of metals by plants is interesting from an environmental

    (bioremediation) or agronomic (biofortification of crops to improve the nutritional value of these

    crops) point of view. In industrial sites, accumulator plants could be used for phytoremediation

    as they are likely able to remove metals from soils (Salt et al., 1995; Salt et al., 1998). This

    therefore, informs the need for the study;

    1. To investigate the heavy metal content of the soil along an effluent channel in a brewery

    industry and its effect on the plant community.


    To evaluate the kind of plants found along the effluent channel and their tolerance level

    to heavy metals as well as remediative abilities.

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    The improper management of the vast amount of waste generated by various

    anthropogenic activities, has become one of the most critical problems of developing countries.

    The most challenging aspect is the way and manner people go about disposing them into the

    immediate environment. This indiscriminate disposal waste is usually carried out on freshwater

    bodies, making them unsuitable for primary and even secondary usage (Fakayode, 2005). In

    1978, the UN reported consumable water levels at 2.7% of earths water, with ground water

    being a major contributor. Present estimates quantify consumable water levels at 1%, ground

    water levels also being threatened by pollution either directly or indirectly (Davis and Cornwell,

    1991). Kehinde in 1996, defined sustainable utilization of the earths wateras the use of water

    resources without any cost whatsoever on future generations, which might arise through misuse

    of the resource.

    This however is no longer the case as brewery, tannery, pharmaceutical, paper and textile

    mills, soap and detergent as well as palm oil mill (POM) effluents are responsible for the

    contamination of this natural surface water in developing and densely populated countries like

    Nigeria (Kanu et al.,2011). Wastewater from Brewery Industry originates from liquors pressed

    from grains and yeast recovery and have the characteristic odour of fermented malt and slightly

    acidic (Kanu et al., 2006). Other wastewater from industries includes employees sanitary waste,

    process wastes from manufacturing, wash waters and relatively uncontaminated water from

    cooling and heating operations (Glyn and Gary, 1996). The increase in the industrialization of

    Benin City has led to pollution stress on surface water (Ajayi and Osibanji, 1981). Industrial

    effluent is unwanted water generated from industrial activities and are inappropriately discharged

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    into the environment or receiving stream. Its characteristics provide basic information about the

    integrity of the rivers and streams into which they are discharged (Kanu et al., 2006). Dada, 1997

    reported that more than 60% of the industries in Nigeria discharged untreated effluent into

    surface water. A study by Uchegbu in 2002 showed that surface water becomes polluted when

    harmful substances (effluents) are released into water bodies from either natural or artificial

    sources. Fellman et al., (1995) reported that manufacturing companies in United States of

    America dumped polychlorinated biphenyls (PCBs) into rivers.

    Effluents from industrial activities contain heavy metals (Bichi, 2000) which may

    damage aquatic ecosystem, health of aquatic animals and those who eat them (The Guides

    Network, 2008). Estuaries and inland water bodies, which are the major sources of drinking

    water in Nigeria, are often contaminated by the activities of the adjoining populations and

    industrial establishments around the water body (Sangodoyin, 1991). Sangodoyin 1995; Ogbeibu

    and Edutie 2002, reported that water bodies (river systems) constitute the primary means for

    disposal of waste thus, altering the physical, chemical and biological nature of the receiving

    water bodies. The initial introduction of waste into water bodies causes the degradation of the

    physical quality of the water, followed by the biological degradation, which becomes seen in

    terms of variety, organization and the number of living organism in the water (Gray, 1989).

    Wastes entering these water bodies are either in solid or liquid forms or both, with great effects

    on the health of the public (Osibanjo et al.,2011). Saad et al., (1984) reported that the increased

    population in many African countries accompanied by a sharp increase in urbanization,

    agricultural and industrial land use, results in a tremendous increase in discharge of a wide

    diversity of pollutants into receiving water bodies. This causes undesirable effects on the

    different components of the aquatic environment.

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    The availability of quality water needed for maintenance of normal biological function is

    on the decline (Odigie and Fajemirokun, 2005). When waste materials are received by the water

    bodies, they are assimilated without significant deterioration of some of the qualities, thus

    implying the assimilative capacity of the water (Fair et al., 1968). This is however due to the self

    purification property of lotic systems (Ifabiyi, 2008). In 2005, studies by Ekhaise and Anyansi

    showed that waste introduces foreign microorganisms, organic and inorganic matter, in addition

    to indigenous microflora, when discharged into the water bodies. Various levels of pollutants can

    be discharged into the environment through public sewer lines based on the type of industry. An

    increase in the levels of pollutants in river water systems causes a rise in biological oxygen

    demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended

    solids (TSS), toxic metals such as Cd, Cr, Ni and Pb and fecal coliform and hence make water

    unsuitable for drinking, irrigation and aquatic life (Emongor et al., 2005; Otokunefor and

    Obiukwu, 2005).

    Organic pollution of inland water systems in Africa, in contrast to the situation in

    developed countries of the world, is often the result of extreme poverty as well as economic and

    social underdevelopment. Countries with the lowest quality and quantity of water, have the

    lowest sanitation and nutrition levels with the worst of diseases most prevalent (Tolba, 1982).

    Worse still, are the very few water quality studies for most African inland waters. The practice of

    effluent discharge in Nigeria, is yet too crude and the society is in danger, especially in the

    industrialized parts of the Cities. Benin is fast becoming a fairly industrialized city, though some

    of these industries are situated some distance away from rivers; their effluents are channeled into

    such rivers as Ikpoba River (Ogbeibu and Edutie, 2002). Some of these industries are soft drink

    industry, alcoholic beverage industry and the wastewater from their operations is conveyed over

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    a distance by an underground tunnel and discharged into Eruvbi Stream, a tributary of Ikpoba

    River. These effluents, which are rich in organic and inorganic substances, are capable of

    producing adverse effects on the physical, chemical and biotic components of the environment

    and either directly or indirectly on human health (Mason, 1981; Ogbeibu and Ezeunara, 2002).

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    3.1 Study Area

    The study was carried out at a site located beside Guinness Nigeria plc, Benin City where their

    effluent channel passes through to the Ikpoba River, along the Benin-Agbor Road, Benin City,

    Edo state.

    Plate 1: The research student on the brewery effluent channel terminating at Ikpoba River, Benin


    Effluent at contact

    point with water

    Brewery effluent


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    3.2 Soil and Plant Collection

    Plant samples were collected using a quadrant method (Trivedy and Goel, 1986). The quadrants,

    1m x 1m was used. Three quadrants randomly placed within 5m along the whole length of the

    channel, and plants were identified and counted. Soil samples were also collected from the

    quadrants and taken to laboratory for analysis in aluminum foil wraps (Anoliefo et al., 2006).

    Plants were identified by using Akobundus 1987 handbook of West Africa weeds.

    Plate 2: The study area around Guinness effluent channel some distance away

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    3.3 Soil Physiochemical Analyses

    3.3.1 Determination of PH

    The pH reading was obtained with the aid of an Hanna microprocessor pH multimeter which was

    earlier standardized with buffer 4.0, 7.0 and 9.0.Twenty (20) grams of the fresh soil sample was

    weighed into a 100 ml glass beaker. Twenty (20) milliters of sterile distilled water was added

    and the suspension was stirred continuously for 30 minutes. The mixture was allowed to stand

    for another 30minutes undisturbed. A Hanna microprocessor pH meter was dipped into the

    solution and steady readings noted (Kalra and Maynard, 1991).

    3.3.2 Electrical Conductivity (EC)

    Twenty (20) grams of the fresh soil sample was weighed into a 100 ml glass beaker. Twenty (20)

    milliters of sterile distilled water was added and the suspension was stirred continuously for 30

    minutes. The mixture was allowed to stand for another 30minutes undisturbed. A Digital

    Conductivity Meter (Labtech) was used in determining soil conductivity by dipping the sensitive

    rod into the mixture and a steady reading taken.

    3.3.3 Total Organic Carbon Content (TOC)

    Air dried soil was passed through a 2 mm sieve in other to remove large particles, roots, organic

    debris and ensure for consistency. This soil sample were used for both carbon and nitrogen

    analyses. A weighed amount (1.0g) of prepared soil sample was dispensed into a 250 ml conical

    flask. Ten (10) mls of Normal Potassium dichromate was added to the flask followed by the

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    addition of 20 ml of concentrated tetraoxosulphate (VI) acid. The flask was shaken for 1 minute

    and allowed to cool. Distilled water was then added to the cold solution to make the volume up

    to 150 ml. This solution was shaken and allowed to cool. Ten (10) ml of phosphoric acid was

    added to the solution followed by the pipetting of 1ml of 1% diphenylamine solution (indicator).

    Titration with 0.5 ferrous ammonium sulphate solutions was done until there was colour change

    from dark violet to green. A blank determination was done for each soil sample (Onyeonwu,



    Blank - Sample Normality of Ferrous Ammonium Sulphate 0.03 1.3 100

    Weight of Sample

    3.3.4 Determination of Total Nitrogen

    The total nitrogen content of the soil samples was determined using micro Kjeldahl digestion and

    colorimetric method (Bremmer and Mulvaney, 1982). One gram soil sample was placed into

    30ml Kjeldahl digestion flask. One tablet of a catalyst (Kjeldahl) and 10 ml concentrated

    H2SO4was added, and the mixture was hand shaken to ensure mixing. At completion of

    digestion, the mixture was clear and upon removal from the digestion chamber, it was allowed to

    cool. Then, 10 ml distilled water was added and the solution was decanted through a Whatman

    filter paper No 42 into a 100ml volumetric flask. The Kjeldahl flask was washed with 2 to 3

    small aliquots of distilled water and all the washings were added into the volumetric flask via the

    filter paper and made up to volume. The nitrogen content of the filtrate was then determined

    colorimetrically. For colorimetric analysis, a standard nitrogen stock solution was prepared using

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    dry ammonium sulphate and from the resultant 100 ppm nitrogen stock solution, 5, 10, 15, 20

    and 25 ppm nitrogen standards were prepared and in each standard, 4 ml concentrated H 2SO4

    and 0.95g anhydrous sodium sulphate was added. A blank solution containing no nitrogen

    standard but having the same quantity of acid and anhydrous sodium sulphate was also prepared.

    Then, 5 ml of the digested filtrate was pipetted into a 25 ml glass flask, and 2.5ml alkaline

    phenol, 1 ml sodium potassium tartrate and 2.5 ml of sodium hypochlorite were added. The

    mixture was hand shaken, and made to 25ml mark with distilled water. The solution was read

    colorimetrically at 630 nm, using a spectrophotometer.

    3.3.5 Available Phosphorus Content

    Five (5) grams of the soil sample was weighed and dispensed into plastic bottle. Forty (40)

    milliters of the extracting solution (0.03M NH4F in 0.025 M HCl) was added and the bottle was

    shaken for 1 minute. The solution was filtered with the aid of a Whatman filter paper No 42. The

    clear supernatant was used for determining the phosphorus content of the respective soil samples.

    Five (5) milliters of the supernatant was pipetted into a 100 ml flask. The pH of the supernatant

    was adjusted to 5 respectively by the addition of 3 drops of p- nitrophenol, and upon the

    development of yellow colour, some drops of 2 M NH4OH were added until a deep yellow

    colour was developed. Also, 2 M HCl was added dropwise until the supernatant became

    colourless. (The resultant pH was between 3 and 5). Thirty (30) milliters of water was added,

    followed by the addition of 10 ml of ascorbic acid reagent. The absorbance of the solution was

    read at 660 nm using a spectrophotometer (Model). (Onyeonwu, 2000)

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    P (mg/kg) = Instrument reading Colour volume Extract volume

    Weight of sample aliquot taken

    3.3.6 Determination of Cation Exchange Capacity (CEC)

    Five (5) grams of air dried soil was weighed into a plastic bottle. One hundred (100) milliters of

    neutral 1 M ammonium acetate was then added to the soil and the mixture was shaken with the

    aid of a mechanical shaker for 30 minutes. The mixture was filtered using a No 42 Whatman

    filter paper into a 100 ml volumetric flask. The filtrate was made up to mark with the acetate.

    Stock working standards 0,2,4,6,8 and 10 ppm were prepared for sodium, potassium, calcium

    and magnesium using 2.54g of oven dried sodium chloride, 100 ml of ammonium acetate, 1.9067

    g oven dried potassium chloride, 0.5004g of calcium carbonate (at 1050C) in 5 ml of

    hydrochloric acid and 0.1216 g of magnesium turning in volume of 6 M hydrochloric acid. The

    concentration of the exchangeable cations (Na, Ca, K and Mg) in the filtrate was determined

    using a flame photometer (Model). The flame photometer was adjusted according to its

    instruction manual and the standards were aspirated to obtain reliable curves before aspirating

    the samples. The blank utilized was ammonium acetate (Onyeonwu 2000).


    Ca (Meq/100g) = Instrument reading 100

    Weight of sample Eq. wt.

    K (Meq/ 100g) = Instrument reading 100

    Weight of sample Eq. wt.

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    Mg (Meq/ 100 g) = Instrument reading 100

    Weight of sample Eq. wt.

    Na (Meq/ 100 g) = Instrument reading 100

    Weight of sample Eq. wt.

    CEC (meq/100 g) = Ca + K + Mg + Na

    3.3.7 Minerals (Metals) Analyses

    The soil sample was spread on a clean plastic sheet placed on a flat surface and air dried under

    room condition for 72hrs. The soil was sieved and 5g sample was taken from the sieved soil and

    put in a beaker. Ten (10) ml of nitric perchloric acid, ratio 2:1 was added to the sample. The

    sample was digested at 105oC. 5ml of HCl was added to the digester again and digested for

    30mins. The digest was then removed from the digester and allowed to cool to room

    temperature. The cooled digest was washed into a 100ml standard volumetric flask and was

    made up to 100ml mark with distilled water. Determination of Iron (Fe), Chromium (Cr),

    Manganese (Mn), Zinc (Zn), Vanadium (V), Arsenic (As), Mercury (Hg), Lead (Pb), Copper

    (Cu), Cadmium (Cd) and Nickel (Ni) were done by aspirating the solution for (analysed) each

    metal analysis into the Atomic Absorption Spectrometer (ASS) PG 550 model (Adelekan and

    Abegunde, 2011).

    3.3.8 Extraction of Nitrate, Sulphate and Ammonium Nitrogen from Soil

    Ten (10) grams of air dried soil was weighed into a plastic bottle. Fifty (50) extraction solutions

    (100g of sodium acetate, and 30ml of acetic acid in one litre of distilled water) were added and

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    the mixture was shaken with the aid of a mechanical shaker for 30 minutes. The mixture was

    filtered using a No 42 Whatman filter paper into a 100 ml volumetric flask. The filtrate was

    made up to mark with the distilled water and preserved for nitrate, sulphate, and ammonium

    nitrogen determination. Nitrate Determination

    Ten milliliter of digest was transfer into fifty milliliter flask, two milliliter of brucine and ten

    milliliter of concentrated sulphuric acid were added. The mixture was mixed and allowed to

    stand for ten minutes. Stock working standards of 0, 2,4,6,8 and 10 ppm were prepared and

    treated in similar way. The optical density (OD) of the samples and standard were taken at

    470nm (Onyeonwu 2000).


    NO3 (mg/kg) = OD x SR x Colour Vol x Ext. vol

    Weight of sample x Vol. taken Sulphate Determination

    Ten milliliter of digest was transfer into fifty milliliter flask, five milliliter of water, one milliliter

    of barium chloride gelatin reagent were added and the solution was allowed to stand for thirty

    minutes, and ten milliliter of concentrated sulphuric acid were added. The mixture was mixed

    and allowed to stand for ten minutes. Stock working standards 0, 2,4,6,8 and 10 ppm were

    prepared and treated in similar way. The optical density (OD) of the samples and standard were

    taken spectrophotometrically at 420nm

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    -S(mg/kg) = OD x SR x Colour Vol x Ext. vol

    Weight of sample x Vol. taken Ammonium Nitrogen Determination

    Five milliliter of digest was transfer into fifty milliliter flask, two and half (2.5ml) milliliter of

    alkaline phenate, one milliliter of sodium potassium tatrate reagent, and two and half (2.5ml)

    milliliter of sodium hypochlorite (parazone) were added. The solution was then shaken. Stock

    working standards of 0, 2,4,6,8 and 10 ppm were prepared and treated in similar way. The

    optical density (OD) of the samples and standard were taken spectrophotometrically at 636nm.


    NH4+-N(mg/kg) = OD x SR x Colour Vol x Ext. vol

    Weight of sample x Vol. taken

    3.3.9 Determination of Metals in Soil/Sediment with AAS Using Wet Acid Extraction

    Method (SRL/AM/S01)

    Determination of Pb, Cu, As, Zn, Ni, Cd, Fe, Mn, Na, K, Ca, Mg and Al in soil and sediment wet

    digestion method.

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    The sample extract was first aspirated into the flame whose high temperature converts the

    analyte atoms into ions in vapour state (exited state). Absorption occurred, when a ground state

    atom absorbed the energy in the form of light of a specific wavelength and was elevated to an

    excited state. The relationship between the amounts of light absorbed by the ion is directly

    proportional to the concentration of the ionic molecules in the solution.

    Samples processing

    The samples were placed in glass Petri dishes and sundry them for 24 hours. After 24 hours of

    drying, any lumps present were broken up with a clean glass rod in order to expose the inside for

    drying.When the samples appeared to be dried, they were left under the sun for further 24 hours

    before grinding. After drying, the soil was grounded. In heavy contaminated soil, it was

    necessary to break up the hard pieces using a mortar and pestle.

    Extraction Procedure

    One gram (1g) of the dried soil sample was transferred into an acid wash 250ml extraction

    bottle. 9ml of concentrated HCl, 3ml of HNO3 and 2ml of perchloric acid were added. The

    mixture was digested for 5-6 hrs on mechanical shaker hot plate. After digestion was completed,

    20 ml if distilled water was added and the solution was filter through a whatman No 42 filter

    paper and finally made up to 100ml. Prepare blank samples using the procedure without any

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    animal sample. The filtrate were analyzed for Heavy metal and trace metal (Cu, As, Zn, As, Pb,

    Cr, V, Ni, Cd, Fe, Mn) using AA PG 550 Spectrometer.

    Calibration and Analysis

    Single elemental standards were prepared by diluting 1000mg/l stock solutions of the respective

    individual elements (Cu, As, Zn, As, Pb, Cr, V, Ni, Cd, Fe, and Mn). A minimum of five

    standard working solutions were prepared daily from the stock Solutions, solution ranged from

    0.1mg/l to 1mg/l. External calibration was used by running demonized water and a suite of

    calibration standards for each element, and Calibration curve was then generated for each

    individual metal. The extracted solutions and blank were then run on the AA to obtain the

    absorbance values, and the concentrations of each metal in the digested samples were

    automatically calculated from the equation of the calibration curve by the AAS equipment.

    Quality Assurance: field acidified demonized water is first aspirated as blanks in duplicates and

    laboratory control samples were run as QC samples.

    SAFTY: Use PVC hand gloves and laboratory coats.

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    4.0 RESULTS

    Physiochemical properties of the soil used in the present study are presented on Table1. Soil used

    in the study had a pH of 6.75, 5.55 and 6.05 in quadrants 1, 2 and 3 respectively. The pH mean

    and standard deviation for the first, second and third quadrants was 6.12 3.52. Total dissolved

    solid for second quadrant was 81.00 mg/kg and for the third was 92.50 mg/kg. Total nitrogen

    was 0.13% for the first quadrant, 0.16% and 0.08% for the second and third quadrants

    respectively. Exchangeable acidity for the first quadrant stood at 0.14mg/100g, 0.72mg/100g for

    the second quadrant and 1.52mg/100g for the third. Heavy metal contents of soil include Fe

    (9.73mg/kg), Mn (0.15mg/kg), Zn (2.47mg/kg), Cr (1.23mg/kg), Pb (0.85mg/kg), Ni

    (0.11mg/kg) and V (0.14mg/kg) for the first quadrant.

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    Table 1: Physicochemical properties of soil used for the present study



    S.I Unit Quadrant 1 Quadrant 2 Quadrant 3 Mean SD

    pH 6.75 5.55 6.05 6.12 3.52

    EC uS/cm 137.00 162.00 185.00 37.2 125.7

    TDS mg/kg 68.50 81.00 92.50 80.67 9.79

    Cl- mg/kg 41.10 48.60 55.50 145.2 96.98

    SO4- mg/kg 42.47 50.22 57.35 50.0 6.07

    NO3- mg/kg 16.44 19.44 22.20 19.36 2.35

    PO4- mg/kg 10.96 12.96 14.80 12.9 1.57

    Na+ mg/kg 4.11 4.86 5.55 4.84 0.62

    K+ mg/kg 6.85 8.10 9.25 8.07 0.83

    Ca+ mg/kg 2.33 2.75 3.15 2.74 0.34


    mg/kg 1.92 2.27 2.59 2.26 0.28Exc. Base meq/100g 1.52 1.79 2.05 1.79 0.2

    Exc. Acid meq/100g 0.14 0.72 1.52 0.79 0.56

    ECEC meq/100g 1.66 2.51 3.57 2.58 0.91

    Fe+ mg/kg 9.73 11.50 13.14 11.5 1.39

    Zn mg/kg 2.47 2.92 3.33 2.91 0.35

    Mn+ mg/kg 0.15 0.18 0.20 0.18 0.02

    Cu+ mg/kg 0.69 0.81 0.93 0.81 0.095

    Ni+ mg/kg 0.11 0.13 0.15 0.13 0.11



    mg/kg 0.27 0.32 0.37 0.32 0.0345V

    + mg/kg 0.41 0.49 0.56 0.49 0.22

    Cr+ mg/kg 1.23 1.46 1.67 2.54 1.12

    Pb+ mg/kg 0.85 1.00 1.15 1 0.13

    Hg mg/kg

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    Plate 3: Plants growing around the effluent channel at the study area

    A combination of all the plant surveyed gave a total of thirteen (13) different species, identified

    in the 3 randomly sampled sites (Table 2 4). A total of 176 plants were collected in the three

    sites visited. From the 49 plants surveyed in quadrants 1, 2 and 3, there were 3 members of the

    Fabaceae family, which included Desmodium scorpiurus, D. tortuosum and Schrankia

    leptocarpa;2 Cyperaceae; Kyllinga squamulataand Cyperus esculentus, 1 member each of the

    Poaceae, Pontederiaceae, Euphorbiaceae, Malvaceae, Limiaceae, Amaranthaceae, Cucurbitaceae

    and Asteraceae families respectively.Poaceae family had the highest number of plants surveyed

    in the three quadrants, with a total of 48 plants, followed by Cyperaceae with 37 plants. Others

    were Euphorbiaceae 26 plants, Amaranthaceae family 20, Limiaceae and Pontederiaceae with 12

    and 11 plants samples respectively. The least number of plants sampled were from the

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    Asteraceae family with a total of 5 plants. Plant species that were moderately distributed

    includes;Hyptis lancoelata, Cyperus esculentus,Luffa aegyptiaca andGomphrena celosiodes.

    Plants that were least distributed in the sites visited are Veronia cinerea Sida acuta and

    Schrankia leptocarpa

    A total of 11 samples ofEichhornia natansappeared in quadrant 1 but not in quadrants 2 and 3.

    Sixteen samples ofKyllinga squamulata,of the Cyperaceae family were surveyed n quadrant 1

    compared to 6 samples surveyed in quadrants 2 and non in quadrants 3. Out of the 49 plants

    surveyed in Q1 (Table 2), Kyllinga squamulata had the highest frequency of 0.33. This was

    followed byEichhornia natansandPhyllanthus amarus with 0.22 respectively.

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    Table 2: Distribution of plant species collected from quadrant 1 (Q1), values show the frequency

    of occurrence of plant species.


    Kyllinga squamulata Cyperaceae 16 12.23 148.8 0.33

    Leptochloa caerulescens Poaceae 3 0.77 0.6 0.06

    Eichhornia natans Pontederiaceae 11 7.23 52.3 0.22

    Phyllanthus amarus Euphorbiaceae 11 7.23 52.3 0.22

    Desmodiumscorpiurus Fabaceae 2 1.77 3.13 0.04

    Cyperus esculentus Cyperaceae 6 2.23 4.97 0.12

    Desmodiumtortuosum Fabaceae 0 0 0 0

    Sida acuta Malvaceae 0 0 0 0

    Hyptis lancoelata Limiaceae 0 0 0 0

    Gomphrena celosiodes Amaranthaceae 0 0 0 0

    Luffa aegyptiaca Cucurbitaceae 0 0 0 0

    Veronia cinerea Asteraceae 0 0 0 0

    Schrankia leptocarpa Fabaceae 0 0 0 0

    TOTAL 49 31.5 262.1

    MEAN 3.77 2.4 20.2

    Variance = 20.2

    Standard deviation =

    = 4.49

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    Table 3 shows the total number of plants surveyed in the second quadrant (Q2). Of the 58

    plants sampled, there were 30 poaceae, 12 cyperaceae and 15 euphorbiaceae. No members of the

    pontederiaceae, malvaceae, limiaceae, amaranthaceae, cucurbitaceae and asteraceae families

    were sampled. The plant with the highest frequency wasLeptochloa caerulescens, of the poaceae


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    Table 3: Distribution of plant species collected from quadrant 2 (Q2), values show the frequency

    of occurrence of plant species.


    Kyllinga squamulata Cyperaceae 6 1.54 2.37 0.10

    Leptochloa caerulescens Poaceae 30 25.5 650.3 0.52

    Eichhornia natans Pontederiaceae 0 0 0 0

    Phyllanthus amarus Euphorbiaceae 15 10.5 110.3 0.26

    Desmodiumscorpiurus Fabaceae 0 0 0 0

    Cyperus esculentus Cyperaceae 6 1.54 2.37 0.10

    Desmodiumtortuosum Fabaceae 1 3.46 11.97 0.02

    Sida acuta Malvaceae 0 0 0 0

    Hyptis lancoelata Limiaceae 0 0 0 0

    Gomphrena celosiodes Amaranthaceae 0 0 0 0

    Luffa aegyptiaca Cucurbitaceae 0 0 0 0

    Veronia cinerea Asteraceae 0 0 0 0

    Schrankia leptocarpa Fabaceae 0 0 0 0

    TOTAL 58 42.54 777.3

    MEAN 4.46 3.27 59.8

    Variance = 59.8

    Standard deviation =

    = 7.73

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    Table 4 shows the plants surveyed in the third quadrant. Gomphrena celosiodeshad the highest

    number of plants surveyed with 20 plant samples. These were followed by Leptochloa

    caerulescens(poaceae) with 15 plant samples and Hyptis lancoelata(limiaceae) with 12 plants


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    Table 4: Distribution of plant species collected from quadrant 3 (Q3), values show the frequency

    of occurrence of plant species.


    Kyllinga squamulata Cyperaceae 0 0 0 0

    Leptochloa caerulescens Poaceae 15 9.69 93.9 0.22

    Eichhornia natans Pontederiaceae 0 0 0 0

    Phyllanthus amarus Euphorbiaceae 0 0 0 0

    Desmodiumscorpiurus Fabaceae 0 0 0 0

    Cyperus esculentus Cyperaceae 3 2.31 5.34 0.04

    Desmodiumtortuosum Fabaceae 0 0 0 0

    Sida acuta Malvaceae 4 1.31 1.72 0.06

    Hyptis lancoelata Limiaceae 12 6.69 44.8 0.17

    Gomphrena celosiodes Amaranthaceae 20 14.69 215.8 0.29

    Luffa aegyptiaca Cucurbitaceae 8 2.69 7.24 0.12

    Veronia cinerea Asteraceae 5 0.31 0.1 0.07

    Schrankia leptocarpa Fabaceae 2 3.31 10.96 0.03

    TOTAL 69 41 379.9

    MEAN 5.31 3.15 29.2

    Variance = 29.2

    Standard deviation =

    = 5.40

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    The report shows the effects of Guinness brewery effluent on the distribution of plants

    along its effluent channel. Some of the effects the indiscriminate dispersal of industrial and urban

    wastes generated by human activities are the contamination of the soil and plant communities

    and the pollution of the aquatic environment, controlled and uncontrolled disposal of wastes,

    accidental and process spillage, mining and smelting of metalliferous ores and sewage sludge

    application to agricultural soils. (Ikhajiagbe et al., 2013). These processes are responsible for the

    migration of contaminants onto noncontaminated sites (Ghosh and Singh, 2005). Unfortunately,

    in developing countries like Nigeria where effluent quality standards imposed by legislation

    (where they exist) are sometimes easily flouted, waste water is indiscriminately discharged into

    water bodies which are the primary receivers (Okereke, 2007). Industrial effluents are liquid

    wastes which are produced in the course of industrial activities. This has however been a major

    concern to both government and industrialist.

    Even though the disposals of effluents are technologically and economically achievable

    for particular standard, industries do not comply with pretreatment requirement. Brewery

    industries produce waste waters like spent cooling water, spent grain and hop liquors, liquor

    from yeast recovery system and wash down water. Waste such as dry brewery grain and spent

    grain are used as animal feedstuff and therefore, do not create disposal problems. Brewery waste

    water can be applied to lands after a limited pretreatment (screening and equalization) if the

    lands are readily available (Otta and Cable, 1987). There was an observed increase in most of the

    parameters studied with little fluctuations in some of the parameters. The introduction of

    wastewater, high in organic matter and essential nutrients brings about changes in the microflora

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    (Rheinheimer, 1991). The physico-chemical parameters investigated showed some variation

    along the sampled sites (Table 1).

    The pH of soil is one of the most important physicochemical parameter. It affects mineral

    nutrient, soil quality and much microorganism activity (Kiran, 2013). It has been found that soil

    pH is correlated with the availability of nutrients to the plant (Gray et al., 1998). Consequently,

    as pH decreases, the solubility of metallic elements in the soil increases and they become more

    readily available to plants (Oliver et al., 1998; Salam and Helmke, 1998). There were slight

    variations in the PH in the three quadrants sampled. The PH values ranged from 5 to 7, an

    indication that effluent from the brewery was slightly acidic. The recorded PH values fell within

    the effluent limitation guidelines and discharge standards, an indication of the basic nature

    (Emmanuel and Jacob, 2013; Kiran, 2013).

    The presence of organic nitrogen in effluent in substantial amount like other tested

    parameters signifies the need for treatment to avoid the associated adverse effects. The values of

    organic nitrogen ranged from 16.44 mg/kg to 22.20 mg/kg (Table 1). These values are slightly

    above the value of 20 mg/l given for effluent discharged in aquatic ecosystem by the FEPA 1988

    but well below the value of 100 mg/L given by General Standard (Emmanuel and Jacob, 2013).

    Many of the ions which are harmless or even beneficial at relatively low concentrations may

    become toxic to plants at high concentration, either through direct interference with metabolic

    processes or through indirect effects on other nutrients, which might be rendered inaccessible. A

    number of elements are normally present in relatively low concentrations, usually less than a few

    mg/kg. These are called trace elements and include manganese (Mn) and vanadium (V). Heavy

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    metals create health hazard when taken up by plants. Heavy metals include copper (Cu), lead

    (Pb), mercury (Hg), arsenic (As), zinc (Zn) and cadmium (Cd).

    Cadmium levels in all the soil samples were found in trace amounts between 0.27 0.37 mg/kg.

    These values are far below the natural limits of 0.01 3.0 mg/kg in soil (MAFF, 1992 and EC,

    1986). The mean level of Pb 1 0.13mg/kg was also, far lower than EC (1986) limits of

    300mg/kg and the maximum tolerable levels proposed for agricultural soil of 90400 mg/kg set

    by the WHO (1993) and NEPCA (2010). Normal range for calcium is between 0.98 2.45 in soil.

    The samples collected from quadrants 2 and 3 (Table 1), were above the normal (Kiran, 2013).

    Electrical conductivity (EC) is a measure of the total ionic composition of soil and its

    overall chemical richness. It is primarily determined in soil by the presence and levels of

    concentration of sodium and magnesium ions and to an extent, calcium ions. Their ions help

    buffer the effect of bicarbonate and carbonate ions, thus maintaining the pH of the soil

    (Ikhajiagbeet al., 2013). The conductivity range of the various quadrants was wide and varied,

    considerably between 137 uS/cm to 185 uS/cm. These values however do not suggest a normal

    soil (Kiran, 2013). The electrical conductivity of the soil is a useful indicator of its salinity or

    total salt content (Ikhajiagbeet al., 2013).

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    Treated brewery effluent discharges onto arable agricultural lands will lead to oxygen depletion,

    increase in plant biomass, decrease in species diversity and changes in the dominant biota of the

    agricultural lands. The studies showed an increase in the concentration of some parameters of

    samples collected close to the brewery, with decreases in concentration further away from the

    brewery. The increase in concentration leads to an increase in biomass as seen in the result in the

    total number of plant species.