Abstract Book

1

2

Book of Abstract Conference on Promotion of Biotechnology in Bangladesh: National and International Perspectives April 6, 7 & 8

Compiled by Mustak Ibn Ayub S M Mahbubur Rashid Md. Mehedi Hasan

Published By Conference Secreteriat: Department of Biochemistry and Molecular Biology University of Dhaka

Printed By Digital Sajghar 34, Northbrook Hall Road, Dhaka-1100 Ph: 01712964636

3

Preamble Biotechnology is a multidisciplinary Science which has the potential to play a major role in National Development. Management and consolidation of Biotechnology activities in the country is therefore essential in order to implement a coordinated and coherent plan for the country’s development. In this context, the recent publication of the ‘Biotechnology Policy’ of the Government through the Ministry of Science, Information and Communication Technology (MoSICT), is a welcome document. However the most important need of the hour is to mobilize this policy into an effective ‘plan of action’ with tangible benefits in the immediate, short-term and long-term future of Bangladesh. This will not only require an effective prioritization of scientific research, but also identification of successful strategies for generating sustainable funding for work in specific areas of Medicine, Health, Agriculture, Environment and Energy and related industries. For the first time in the country, the Government, Public and Private Universities, a Major Science Academy and the Private Sector are jointly organizing this 3-day conference to brainstorm and put ideas forward in an effort to promote Biotechnology in Bangladesh. The Ministry of Science, Information and Communication Technology, University of Dhaka, BRAC University, Bangladesh Academy of Sciences, International Center for Diarrhoeal Disease and Research (ICDDR,B), Square Pharmaceuticals, Incepta Pharmaceuticals and East West Seed (Bangladesh) Ltd. are sponsoring this major event. The participation of other relevant government agencies, academic and research institutes, both public and private, banking institutions, and the private sector, are also being welcomed. It is hoped that the deliberations of the conference will result in the following actions:

1. Scientific capacity assessment within the country in terms of identifiable research outputs of government, non-government and private institutes working on Biotechnology-related activities. Strengthening, management and appropriate networking of existing research institutes and universities. Effective partnership with government agencies and industry for delivering research output. Strategies for upgrading 1-2 selected institutes into Centers of Excellence, e.g. the National Institute of Biotechnology, NIB.

2. Establishment of International Linkages with Scientific Centers of Excellence and arranging collaboration with the help of Expatriate Scientists. Identification of local areas of strength as well as need, which could attract foreign funding and collaboration.

3. Prioritization of research goals and activities and strategies for implementation of deliverable outputs in tune with some of the Millennium Development Goals.

It is expected that the deliberations of the conference will produce a targeted plan of action for biotechnological research activities for National Development. The plan should lead to tangible outputs through cooperation, networking and coordination, not only among local and expatriate scientists, but also with the Government, industry and end-users for the welfare of the general population of Bangladesh. The idea for the conference was initiated by Expatriate and Local Senior Scientists and the forum of Bangladeshi Biotechnologists built through the website, Global Network of Bangladeshi Biotechnologists, or GNOBB on a purely voluntary basis. This site has played a crucial role in bringing the Bangladeshi Biotechnology Community all over the world together over the past two years. The multifaceted activities of Bangladeshi Biotechnologists and important advances in the diverse fields of biotechnology have been regularly highlighted in this website. GNOBB was also responsible for bringing the student community together through the formation of the Young Bangladeshi Biotechnologists web-based group, YoungBB. A one day preliminary seminar also took place successfully at the Center of Excellence, University of Dhaka on the 28th December, 2006 to initiate the activities for the current 3-day conference. The response to the call of the conference from Biotechnologists within Bangladesh has been overwhelming. More than 300 participants have already registered for the conference. We were unable to

4

accommodate many undergraduate students, who were eager to participate. Many expatriate scientists have agreed to participate voluntarily and will be presenting their work. Some scientists unable to physically participate are providing information through the survey questionnaire, which will be published in the book of abstracts. We are very sorry that we were unable to accommodate many oral presentations because of time constraints in this 3-day promotional and scientific conference. Well known Scientific Leaders from India, Pakistan, Turkey and Malaysia are joining us to share their experience and help us in our endeavors. COMSTECH and ICGEB have joined our efforts to make this important participation possible. Messages from some expatriate and foreign well-wishing scientists have been included in the book of abstracts. Abstracts have been published as received from the participants, without any editing. The abstracts have been named after the scientist who communicated with us. In a few cases, we were unaware whether the communicator was a student or professor-we hope that any error in this regard will be overlooked. The effort of expatriate scientists, conference conveners, conference chairs, chairs of other committees, Committee members, senior scientists at the venue, ICDDR,B and most of all the young and enthusiastic volunteers at the conference secretariat have been crucial in organization of the conference. Grateful mention must also be made of the young student webmasters of the conference website, promotebiotechbd.net. However organizational lapses may occur and request is being made to the participants to overlook these and join hands to make the conference a resounding success. Conference Secretariat

5

Brief Contents

Program 05 - 11 Oral Presentations 14 - 73 Poster Presentations 74 - 141 Expatriate and Foreign Scientists Abstracts and Messages

142 - 145

Questionnaire 146 - 230 Conference Committees 231 - 239 Index of Scientists’ Name 240 - 242

6

PROGRAM Conference on

Promotion of Biotechnology in Bangladesh: National and International Perspectives

April 6-8, 2007, Dhaka, Bangladesh Day-1 April 6, 2007: Venue: ICDDR, B, Sasakawa Auditorium Inaugural Session: 9:00 - 10:10 Chief guest Honorable Mr. Tapan Chowdhury, Advisor, MoSICT Special Guest: Dr. C. S. Karim, Honorable Adviser, MoA Prof. A.S. Islam Retired Professor, Botany, DU

Recitation of verses from the Holy Qur’an relating to the theme of the Conference and their Translation

Prof. Jamilur Reza Choudhury Vice Chancellor, BRAC University

Welcome Address and conference objective

Prof. Naiyyum Choudhury Secretary, Bangladesh Academy of Sciences (BAS)

Current Status and Prospects for Biotechnology in Bangladesh

Dr. David Sack Executive Director, ICDDR,B

Speech by Guest of Honor

Dr. S.M.A Faiz Vice Chancellor, Dhaka University


Prof. Shamsher Ali President, BAS


Dr. C. S. Karim Honorable Adviser, MoA

Speech by the Special guest

Mr. Tapan Chowdhury Honorable Adviser, MoSICT

Inaugural address by the Chief Guest

Tea: 10:10 - 11:00 Scientific Session 1: 11:00 -13:00 Chair: Dr. A. K. Azad Khan, Secretary General, Diabetic Association of Bangladesh Co-Chair: Dr. Anwar Nasim, Adviser Science, COMSTECH, Pakistan Prof. Ahmed A. Azad (Scientific Adviser (CSA), ICGEB and TWAS Research Professor, BRAC University)

Novel Therapeutic Strategies against AIDS Progression based on the Structure and Function of HIV-1 Pathogenic Proteins Nef and Vpr.

Dr. S. M. Faruque (Head, Molecular Genetics, ICDDR, B)

Potential For Biotechnology In Environmental Intervention To Prevent Epidemic Diseases: Cholera As A Model

Dr. Alam Nur-É-Kamal (Associate Professor, Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, USA)

Embryonic Stem Cells: Potential Use in Tissue Engineering and Regenerative Medicine

Dr. Lamia Sharmeen (Nanoderm Therapeutics, Founder and CSO, USA)

Drug Discovery Knowledge And Application: Strategies to Reinvent The R&D Business Model

LUNCH 13:00-14:15

7

Scientific Session 2: 14:15-16.45 Chair: Prof. S. Hadiuzzaman, Botany, DU Co-Chair: Dr. C.M. Hasan, Chairman, BCSIR Dr. Abed Chaudhury (CSIRO, Australia)

Biotechnological Approaches in Hybrid Development

Dr. Firdausi Qadri (Head, Immunology, ICDDR,B)

Vaccine Research, Development and Production: an important strategy for Reducing Infectious Diseases in Bangladesh

Prof. A.K. Azad Choudhury (Pharmacy, DU)

Biotechnology to Solve some Local health Problems: Arsenicosis, Molecular Characterization of Pathogens, Emergence of Multi Drug Resistance and Development of Drugs for Multidrug Resistant Pathogens.

Prof. M. Anwar Hossain, (Biochemistry and Molecular Biology and Dean, Biological Science Faculty, DU)

Biosensors for Pesticide Detection in Fruits and Vegetables

Prof. Haseena Khan (Biochemistry and Molecular Biology, DU)

Understanding Jute at The Molecular Level

TEA 16.45-17:00 Scientific Session 3: 17:00-18.15 Chair: Dr. Abed Chaudhury, PI, CSIRO, Australia Co-Chair: Dr. Zafar Iqbal Dr. Abul Ekramoddoullah (Canadian Forest Service, Victoria, Canada)

Proteomic Approach to Study Forest Tree-Pathogen Interaction

Prof. Zeba Seraj (Biochemistry and Molecular Biology DU.)

Approaches to Production of Salt Tolerant Rice for Coastal Region of Bangladesh

Prof. R.H. Sarker (Botany, DU.) Improvement of Grain Legumes

POSTER SESSION*** 18:15-19:15 Exhibition all day Cultural Program & Buffet Dinner hosted by East West Seeds: 19:15

8

Day-2 April 7, 2007: Venue: ICDDR, B, Sasakawa auditorium Session 1: 09:00-11.00 Biotechnology in developing countries Chair: Mr. Sayeduzzaman, Chairman, Bangladesh Rice Foundation Co-Chair: Dr. G. B. Nair, ICDDR,B Prof. V. S. Chauhan (Director, ICGEB, India)

Biotechnology in India: An ICGEB Experience

Dr. Anwar Nasim (Advisor, COMSTECH, Pakistan)

Biotechnology Initiatives In Pakistan

Prof. Mehmet Ozturk (Molecular Biology and Genetics, Bilkent University, Turkey)

Life Sciences And Biotechnology In Turkey

Dr. Asma Ismail (Director, Inst. Research Molecular Medicine, Malaysia)

Integrating excellence in molecular science and technology to generate Knowledge-based industries: A Malaysian experience in diagnostics

Open Discussion & TEA: 11.00- 11:30 Session 2: Capacity Development I 11:30 -13:15 Chair: Major General Dr. A. S. M. Matiur Rahman (Rtd.) Co-Chair: Dr. Naiyyum Choudhury

Prof. Ahmed A. Azad (Scientific Adviser, ICGEB and TWAS Research Professor, BRAC University)

Biotechnology Capacity Development in scientifically lagging countries of Asia and Africa through the establishment of a Drug Discovery and Development Research Network based on Traditional Knowledge and Indigenous Resources

Prof. Yusuf Haider (ProVC, DU) Research prospects at the Centre of Excellence, DU

Mr. S. M. Wahid-uz-Zaman (Secretary, MoSICT)

Coordination and funding for Biotechnology Research

Mr. A. Muktadir (MD, Incepta Pharmaceuticals)

Prospects for biopharmaceutical R&D in Bangladesh

Dr. A. K. Azad (Biochemistry, NICVD)

National Guidelines on Medical Biotechnology in Bangladesh

LUNCH: 13:15-14:15

9

Session 3: 14:15-16:00 Capacity Development II Chair: Dr. C.S. Karim, Adviser Co-Chair: Prof. Serajul I. Khan, Prof. Microbiology, DU Dr. Jalaluddin Bhuiyan (Head, Clinical Biocem., King Faisal Hospital, SA)

Importance of Laboratory Tests to a Country’s Healthcare System and its Accreditation – What It Is and How to Get It?

Dr. M.A Razzak (Member-Director, Crops, BARC)

Overview of Policy and Research in Agricultural Biotechnology

Mr. Awal Mintoo (Chairman, East West Seeds)

Social and Economic Dimension of Agricultural Biotechnology

Prof. Mushtaque Choudhury (Dean, BRAC School of Public Health) BRAC’s Biotechnology Vision for BD

TEA: 16:00-16:15 Session 4: 16:15-17:45 Research Partnerships, Biosafety, Bioethics, IPR etc. Chair: Dr. David Sack, Executive Director, ICDDR, B Co-Chair: Dr. Ainun Nishat, IUCN Prof. A.S. Islam (Retired from Botany, DU) GMOs and Biosafety

Mr. Md. Solaiman Haider (Assistant Director (Technical) Department of Environment)

Cartagena Protocols on Biosafety and its implementation activities in Bangladesh

Mr. Michael Behan (ICDDR, B)

Intellectual Property Rights, Innovation, Trade Laws and Access

Dr. Sharif Akhteruzzaman (Forensic Science, DMC)

DNA Technology in Forensic Science: Bangladesh Perspective

Ms. Nafisa Akhter Rouf (Executive, Square Agrobiotech) Agrobiotechnology and Square

Dr. Shah Md. Razzaque (BRAC Biotech Laboratory, Joydebpur) Plant Tissue Culture at BRAC ARDC

POSTER SESSION*** 17:45-19:00 Exhibition all day; Chairs and committee for selection of best posters Prof. Rafiqur Rahman, Prof. Ziauddin Ahmad, Prof. M. Imdadul Hoque, Dr Prof. Mozammel Hoq, Prof. Sayedul Islam, Prof. Choudhury Rafiqul Ahsan

FORMAL DINNER 19:00

10

Day-3 April 8, 2007: Venue: ICDDR, B, Sasakawa Auditorium Session 1: 09:00-11:00 Research Institutes: Current and Future Activities Chair: Prof. Saleheen Qadri, Director CoE, DU Co-Chair: Prof. Mamunur R. Chowdhury, DU Prof. M. A. Aziz (Project Director, National Institute of Biotechnology)

Prospects of research at NIB

Dr. G. B. Nair (ICDDR,B) New Frontiers in Laboratory Sciences at ICDDR,B Dr. Liaquat Ali (BIRDEM)

Biochemical and genetic characterization of young onset diabetes in Bangladeshi population

Dr. C.M. Hasan (Chairman, BCSIR) Present Status of Biotechnological Research in BCSIR

Prof. M. A. Rashid (Dean, Faculty of Pharmacy, DU)

Present and Future Research Perspectives in Herbals, Pharmaceuticals, Biopharmaceuticals and Biotechnological Areas

Dr. Samir Kumar Saha (Shishu Hospital)

Resource-poor Microbiology Laboratories in Bangladesh: Prospects of Biotechnology

TEA: 11:00- 11:30 Session 2: Oral Presentations (3 concurrent sessions) 11:30-13:30 Concurrent 1 : Sasakawa Auditorium Chair: Prof. Mustafizur Rahman, DU Co-Chair: Prof. Md. Shahjahan, RU Dr. Firoz Alam (Botany, RU), Biotechnology for Rice and Solid Waste Decomposition Prof. M.A. Rahim (Horticulture, BAU)

Germplasm Center (GPC)-The largest fruit genetic resources for biotechnological research

Prof. Lutfur Rahman (BAU) Morpho-Molecular Characterization of Plant Varieties of Bangladesh for Variety Protection

Prof. Md. Golam Rabbani (BAU) Molecular Characterization of Landraces and Wild relatives of Some Important Vegetables and Fruits of Bangladesh

Dr. Lutful Hassan (BAU) Generation of Bioengineered Rapeseed for Salt Tolerance

Dr. Aparna Islam (BRAC University) Plant Defensin for Improving Plant Resistance

Dr. A.F.M. Jamal Uddin (BAU) Phylogenetic Relationship of Phytophthora citrophthora Isolates based on rDNA Internal Transcribed Spacer Sequence Analysis.

Dr. M.M. Islam (BINA) A Classical and Molecular Analysis: Are Genes for Salinity Tolerance at Seedling and Reproductive Stages in Rice the Same or Different?

Dr. Md. Rafiqul Islam (BRRI) Application of Molecular Markers to Rice Breeding Populations and Haplotype Diversity for Salinity Tolerance in Chromosome 1

Dr. S. H. Prodhan (Tsukuba) Salt Tolerant Rice Development through Genetic Engineering

11

Concurrent 2: Seminar Room 1

Chair : Prof. Ishtiaq Mahmud, DU Co-Chair: Prof. Anwar Hossain (Microbiology, DU) Dr. M. A. Mazid (Pharmacy, DU)

Generation of Monoclonal Antibody for Prostaglandin D2 Using Stable, Isoteric Analogue as an Hapten Mimic and its Application

Dr. Rashidul Haque (ICDDR, B)

Molecular Diagnostic Tests for Enteric Protozoan Parasites: Recent Developments

Dr. Apala F. Naved (BMB-DU)

Screening For Lymphatic Filariasis and Development Of Indigenous Molecular Diagnosis.

Dr. Rubhana Raqib (ICDDR,B) Evaluation of Antibodies in Lymphocyte Supernatant- New Hope for Diagnosis of Pediatric TB.

Dr. Md. Bakhtiar Hossain (ICDDR, B)

Effects of Iron and Zinc Supplementation during Pregnancy on Neonatal Iron Status in Rats.

Dr. Anwarul Azim Akhand (GEB, DU)

Arsenic-mediated Apoptosis of Murine T Lymphocytes involving Activation of JNK

Dr. M. Alimul Islam (BAU) Molecular Characterization and Clinical Evaluation of Dengue Outbreak in 2002 in Bangladesh

Nadim Ashraf (UK) Current Advances In The Formulation And Delivery Of Insulin

Dr. Md. Shahidur Rahaman Khan (Microbiology, BAU)

Molecular Characterization of the Major Outer Membrane Protein of Haemophilus somnus

Concurrent 3 : CSD Auditor Chair: Prof. H. K. M. Yusuf Co-Chair: Prof. Laila Noor Islam Dr. Arifuzzaman (Dept. of Biochemistry and Biotechnology, USTC)

Large scale Identification of Protein-Protein Interaction of E.coli K-12

Abdullah-Al-Mueen (Bioinformatics, BUET)

A Heuristic Algorithm for Individual Haplotyping with Minimum Error Correction

Dr. A.K. Fazlul Haque Bhuiyan (Animal Breeding, BAU)

Characterization, Conservation and Improvement of Red Chittagong Cattle of Bangladesh

Dr. Khan Shahidul Haque (BLRI) Development of Herbal Feed Additives for Ruminants

Dr. Ananda K. Saha (Zoology, RU)

Biodegradation of Effluents of Tannery Wastes

Dr. Md. Amzad Hossain (BSRI) Tissue culture: An effective Biotechnological Tool for Propagation, Production and Utilization of Stevia at Bangladesh Sugarcane Research Institute

Dr. Md. Mukhlesur Rahman Khan (Fisheries, BAU)

Genetic Variation of Wild and Hatchery Populations of Indian Major Carp,Catla (Catla Catla Hamilton) revealed by RAPD markers

Dr. Md. Azharul I. Talukder (BLRI) Study on RAPD Analysis of Selective Goat Population

Dr. Monzur Hossain (RU) Development of Somaclonal Variants of Strawberry, Adaptive to the Agro-Ecological Condition of Bangladesh

Dr. M. Aminul Haque (RU) Development and Evaluation of Transgenic Lettuce Expressing Salmon calcitonin (sCT) and Human calcitonin related peptide (hCGRP) Genes.

12

LUNCH 13:30-14:30 Session 3: 14:30-16:00 Improvement of Research Productivity and Infrastructure Chair: Mr. Mahfuz Anam Co-Chair: Dr. Firdausi Qadri, ICDDR, B Dr. A.S. Islam (Retired Professor, Botany, DU)

Presentation of Draft Position Paper

Open Discussion and Recommendations

TEA: ` 16:00-17:00 (Finalization of Recommendations by the Implementation Committee during tea break) CONCLUDING SESSION 17:00- 17:30 Chief Guest: Major General (Retd) Dr. A.S.M. Matiur Rahman Co-Chair: Dr. A. S. Islam Prof. A. Azad (Conference Chair) Presentation of Final Recommendations and Press Release

Prof. Jamilur Reza Choudhury (Chairperson of Preparatory Committee)

Closing remarks

Prof. Haseena Khan (Secretary, OC) Vote of thanks

PRESS RELEASE 17:30-18: DINNER: Invited Speakers, Sponsors, Chairs/Co-Chairs, Preparatory Committee

13

14

Abstracts

Oral Presentations

A. Scientific (S) B. Promotional (Pr)

Poster Presentations (P)

15

Abstract

Oral Presentations

16

Detailed Contents of Oral Presentations

(The recieved abstracts are arranged according to their presentation sequence in the program)

A. Scientific

Category Name of Author Subject Page No.

S-1 Ahmed A Azad

Novel Therapeutic Strategies against AIDS Progression based on the Structure and Function of HIV-1 Pathogenic Proteins Nef and Vpr

19

S-2 Alam Nur-E-Kamal Ph.D.


20

S-3 Lamia Sharmeen, PhD

Drug Discovery knowledge and application: Strategies to reinvent the research and development business model

21

S-4 A.K. Azad Chowdhury, Sheikh Zahir Raihan, M. Asaduzzaman, Nishat Nasreen and Nahid Akhter, A. K. M. Shahidur Rahman, Mohammad Shawkat Ali & Golam Hasan Rabbani, Mahbubur Rahman, and Kaiser Ali Talukder

Biotechnology to solve some local health problems: arsenicosis, molecular characterization of pathogens, emergence of multi drug resistance and development of drugs for multi drug resistant pathogens

22

S-5 M. Anwar Hossain, Kagan Kerman, Naoki Nagatani, Akihiro Takeuchi, Teruko Yuhi,Tatsuro Endo, , Yuzuru Takamura and Eiichi Tamiya

Biosensors for pesticide detection in fruits and vegetables

26

S-6 Nadim Ashraf, Aleya Awal, Samiul Haque, Monira Obaid, Rokeya Sultana, Saaimatul Hoque, Shakinur Islam Mondal and Haseena Khan

Understanding Jute at the Molecular Level

27

S-7 Abul K M Ekramoddoullah


28

S-8 Zeba I. Seraj

Approaches to Production of Salt Tolerant Rice for Saline Costal Region of Bangladesh

29

S-9 R. H. Sarker, Rehana Hashem and M. I. Hoque

Improvement of Grain Legumes Through Genetic Transformation

30

S-10 M. Firoz Alam, M. Khalekuzzaman, S. Mahfuja Khatun, Swapan K. Datta and Philippe Herve

Biotechnology for Rice and Solid Waste Decomposition

31

17

S-11 M. A. Rahim, H. R. M. Masud Anwar, M. S. Alam, M. A. Kabir, B. C. Sarker, M. S. Bari, N. Naher, F. Islam, D. A. N. Majumder

Germplasm Center (GPC)-The largest fruit genetic resources for biotechnological research

33

S-11 Lutfur Rahman, Rezwan Mollah, Sayeda Sulata, Mohammad Nazrul Islam, Nesar Uddin Ahmed, Md. Shefatur Rahman, Md. Nazim-ud-Dowla , M.Shah-e-Alam, and M.S.Alam

Morpho-molecular Characterization of plant varieties of Bangladesh for plant variety protection

34

S-12 M.G. Rabbani, M. A. Rahman and E.J. Garvey

Molecular Characterization of Landraces and Wild relatives of Some Important Vegetables and Fruits of Bangladesh

35

S-13 Lutful Hassan and S.K. Talukder Generation of bioengineered rapeseed for salt tolerance

36

S-14 Aparna Islam and V. S. Reddy Plant Defensin For Improving Plant Resistance

37

S-15 A.F.M. Jamal Uddin, M. Senda, S. Uematsu, and K. Kageyama

Phylogenetic relationship of Phytophthora citrophthora isolates based on rDNA internal transcribed spacer sequence analysis

38

S-16 M.M. Islam and G.B. Gregorio

A Classical and Molecular Analysis: Are Genes for Salinity Tolerance at Seedling and Reproductive Stages in Rice the Same or Different?

39

S-17 M.R. Islam, G.B Gregorio, B.C.Y. Collard, E. Tumimbang-Raiz, D.L. Adorada, R.D. Mendoza, M.A. Salam, and L. Hassan

Application of Molecular Markers to Rice Breeding Populations and Haplotype Diversity for Salinity Tolerance in Chromosome 1

40

S-18 Shamsul H. Prodhan, K. Nakao, K. Nagamiya, T. Motohashi, S. Jesmin, A. Komamine and H. Morishima.

Salt Tolerant Rice Development Through Genetic Engineering

41

S-19 M. A. Mazid, M. A. Rashid, A. A. Chowdhury, K. Nishimura, M. Jisaka, T. Nagaya, and K. Yokota

Generation of Monoclonal Antibody for Prostaglandin D2 Using the Stable, Isosteric Analogue as an Hapten Mimic and its Application

42

S-20 Rashidul Haque

Molecular diagnostic tests for enteric protozoan parasites: Recent Developments

43

S-21 Apala Farhat Naved Screening endemicity for lymphatic filariasis (LF) and development of indigenous molecular diagnostics Running Title- LF endemicity & indigenous molecular diagnostics

44

S-22 Rubhana Raqib, Dinesh Mondal, Anwarul karim, Fahima Chowdhury, Sultan Ahmed, Sanaul Bashar, Stephen Luby, Jan Andersson, David Sack.

Evaluation of antibodies in lymphocyte supernatant- New hope for diagnosis of pediatric TB.

45

18

S-23 Mohammad Bakhtiar Hossain, Shannon L Kelleher, and Bo L Lonnerdal.

Effects of iron and zinc supplementation during pregnancy on neonatal iron status in rats.

46

S-24 Anwarul Azim Akhand, Khaled Hossain and Izumi Nakashima

Arsenic-mediated apoptosis of murine T lymphocytes involving activation of JNK

47

S-25 M.A. Islam, M. U. Ahmed , N. Begum , N. A. Chowdhury , A. H. Khan , M. C. Parquet , S. Bipolo , S. Inoue , F. Hasebe , Y. Suzuki and K. Morita

Molecular Characterization and Clinical Evaluation of Dengue Outbreak in Year 2002 in Bangladesh

48

S-26 Nadim Ashraf and Gary Adams Current Advances in Insulin Delivery and Formulation

49

S-27 M.S.R. Khan, A. Tanaka A,H Ide, K Hoshinoo, Y Hanafusa , Y Tagawa2


50

S-28 Mohammad Arifuzzaman, Maki Maeda, Rintarou Saito, Shigehiko Kanaya, Chieko Wada, Hirotada Mori

Large-Scale identification of protein-protein interaction of Escherichia coli K-12

51

S-29 Abdullah Al Mueen, Md. Shamsuzzoha Bayzid, Md. Maksudul Alam and Md. Saidur Rahman


52

S-30 K. S. Huque and Nasrin Sultana Development of herbal feed additives for ruminants

53

S-31 A.K.Saha, M.A.Mannan , and M.K. Mahanta

Biodegradation of tannery effluents by bacteria

54

S-32 Dr. Md. Mukhlesur Rahman Khan

Genetic variation of wild and hatchery populations of Indian major carp, catla (Catla catla Hamilton) revealed by RAPD markers

55

S-33 M.A.I. Talukder; B.B. Roy and K.M. Hossain

Study on RAPD Analysis of Selective Goat Population

56

S-34 M. Hossain, U.K. Roy, R. Karim, MK Biswas, N Nahar, A Hoque and R Islam

Development of Somaclonal Variants of Strawberry Adaptive to the Agro- ecological Condition of Bangladesh.

57

S-35 A. Hoque, M. Hossain, MB. Ahmed, MK. Biswas and R. Islam

Development and Evaluation of Transgenic Lettuce Expressing Salmon calcitonin (sCT) and Human calcitonin related peptide (hCGRP) Genes.

58

19

B. Promotional

Category Name of Author Subject Page No.

Pr-1 Prof. Virander Singh Chauhan


61

Pr-2 Dr. Anwar Nasim Biotechnology Initiatives in Pakistan 62 Pr-3 Mehmet Ozturk, Ph. D. Life Sciences And Biotechnology in

Turkey 63

Pr-4 Prof Asma Ismail

Integrating excellence in molecular science and technology to generate Knowledge-based industries: A Malaysian experience in diagnostics

64

Pr-5 Prof. Ahmed A. Azad

Biotechnology Capacity Development in scientifically lagging countries of Asia and Africa through the establishment of a Drug Discovery and Development Research Network based on Traditional Knowledge and Indigenous Resources

65

Pr-6 Dr. A. K. Azad


66

Pr-7 Dr. Jalaluddin Bhuiyan

Importance of Laboratory Tests to a Country’s Healthcare System and its Accreditation – What It Is and How to Get It.

67

Pr-8 Dr. Md. Abdur Razzaque Biotechnology Policy, Challenges and Opportunities and Research at Agricultural Research Institutes: An Overview

68

Pr-9 Mr.Michael Behan


69

Pr-10 Dr. Sharif Akhteruzzaman


70

Pr-11 Dr. Liaquat Ali Biochemical and Genetic Characterization of Young Onset Diabetes in Bangladeshi Population

71

Pr-12 M. A. Rashid, M. A. Mazid, M. S. Rahman, M. R. Haque


72

Pr-13 Samir K. Saha, Ph.D.

Resource-poor microbiology laboratories in Bangladesh: Prospects of Biotechnology.

73

20

Novel Therapeutic Strategies against AIDS Progression based on the Structure and Function of HIV-1 Pathogenic Proteins Nef and Vpr

Ahmed A Azad1

HIV/AIDS kills more people than any other infectious disease, and causes devastation to the health and economies of the poorest and least developed countries of the world that are least able to afford the currently used retroviral therapy that has had a dramatic effect in reversing AIDS-related morbidity and mortality in the developed countries. While the world eagerly awaits an effective prophylactic vaccine, there is an urgent and on-going need to slow the infection rate and provide relief to the 45 million HIV-infected people. There is, therefore, an urgent need for new drugs and therapeutic vaccines, for the infected population, that are effective, affordable and patient friendly, and that help to slow progression to full blown AIDS. We have presented evidence to show that HIV-1 accessory proteins Nef and Vpr could be involved in AIDS pathogenesis. When present in the extracellular medium, Nef and Vpr cause the death of uninfected (bystander) cells, and may, therefore, be responsible for the depletion of immune cells in lymphoid tissues during HIV infection. When present inside the cell they prevent the death of infected cells and could, therefore, contribute to increase in viral load. Neutralisation of extracellular Nef and Vpr should prevent the death of uninfected immune cells and thereby the destruction of the immune system. Neutralization of intracellular Nef and Vpr should hasten the death of infected cells and help reduce the viral load. Nef and Vpr are, therefore, very important molecular targets for developing therapeutics that slow progression to AIDS. Nature has pointed to some very useful potential drugs. The N-terminal region of Nef and naturally-occurring Bee Venom Mellitin have very similar primary and tertiary structures, and they both act by destroying membranes. Chemical analogues of a Mellitin Inhibitor prevent Nef-mediated cell death and inhibit the interacton of Nef with cellular proteins involved in apoptosis. Naturally occurring Bee Propolis also contains substances that prevent Nef-mediated cell lysis, and increases proliferation of CD4 cells in HIV-infected cultures. Simple bioassays, based on the pathogenic effects of Nef and Vpr, have been developed in my laboratory. These can be used in Bangladesh to screen natural product libraries held by Bangladeshi laboratories to discover lead compounds and develop new IP. Bangladeshi scientists could choose to join a drug discovery and development research network being set up in the OIC region to optimize the lead molecules into candidate drugs. 1TWAS Research Professor, BRAC University, Dhaka, Bangladesh [email protected]

21


Alam Nur-E-Kamal Ph.D.1

Embryonic stem cells (MESCs) are permanent cell lines derived from the inner cell mass of blastocysts. Embryonic stem cells possess the ability to grow in cell culture condition and differentiate into all types of body cells under appropriate conditions. The regulation of embryonic stem cell fate is controlled by the interplay of signaling networks that either promote self-renewal or induce differentiation. Leukemia inhibitory factor (LIF) is a cytokine demonstrated to be a requirement for stem cell renewal in mouse but not in human embryonic stem cells. However, feeder layers of embryonic fibroblasts are capable of inducing stem cell renewal in both cell types, suggesting that the self-renewal signaling pathways may also be promoted by other triggers, such as alternative cytokines and/or chemical or physical properties of the ECM secreted by feeder fibroblasts. We have recently developed a synthetic nanofibrillar matrix whose three dimensional organization resembles the ECM/basement membrane. Growth of mouse embryonic stem cells on these three dimensional (3D) surfaces greatly enhanced proliferation and self-renewal in comparison to growth on two dimensional (2D) surfaces, despite the presence of LIF in both systems. Enhanced proliferation and self-renewal of the stem cells on 3D surfaces was correlated with the activation of the small GTPase Rac, the enhanced expression of nanog, a homeoprotein which is required for maintenance of pluripotency; and the enhanced expression of c-Fos, a transcription factor involved in the regulation of cell cycle progression, differentiation, and apoptosis. Use of inhibitors of PI3K demonstrated a decrease in the expression level of nanog for mouse embryonic stem cells cultured on 3D surfaces. 3D polyamide nanofibers also stimulated human embryonic stem cell proliferation. We have developed condition to direct differentiation of mouse and human embryonic stem cell to brain and pancreatic precursor cells. These precursor cells are now being used to produce mature brain and pancreatic cells. Potential application of differentiated embryonic stem cells will be discussed. 1Department of Pharmacology, Robert Wood Johnson Medical School-UMDNJ, 675 Hoes Lane, Piscataway, NJ 08854, USA

And Department of Biology, MEC-City University of New York, New York 11225, USA

22

Drug Discovery Knowledge and Application: Strategies to Reinvent the Research and Development Business Model

Lamia Sharmeen, PhD1

The primary objective of drug discovery is to utilize knowledge with a purpose to transfer its benefit from bench side to patients. Transferring bench research to patients or translational research is driving the modern drug discovery. This lecture will present few examples of G-protein coupled receptors (GPCRs) and cell signal molecules to illustrate how scientific ideas can be transferred into discoveries of new therapeutic molecules. The CC chemokine receptor 5 (CCR5) and CXCR4 are used by the human immunodeficiency virus (HIV) to infect cells. Strategies that target human CCR5 were therefore developed to prevent and treat HIV infection. Two novel series of aminal and indole-amine compounds were identified as potent and selective inhibitors of HIV infection. Similarly, clozapine, targets a GPCR, acetylcholine muscarinic receptor, and it was approved in 1989 by US Food and Drug administration as first atypical anti-psychotic for its effectiveness in drug refractory patients. However, it causes agranulocytosis in some patients leading to death. Recent work has led to a new understanding of the mechanism of action of this compound. One of the metabolites of clozapine, n-desmethylclozapine functions as muscarinic M1 subtype selective allosteric agonist and this property could be responsible for its superior activity. Many drug discovery targets may not be accessible to small chemical molecules. A small peptide sequence (Ac-PHSRN-NH2) derived from cell fibronectin protein, functions like a signal molecule to enhance cell invasion and migration leading to rapid wound closure and reepithelization in wound model of obese diabetic mice. One of our current research goals is to develop this early discovery product by proving its efficacy in diabetic ulcer patients and develop a nanoparticles encapsulated topical formulation for drug delivery. New paradigm in modern drug discovery empowered by biotechnology tools like genomics, proteomics and bioinformatics is shifting research from small chemical molecule to biologics, and RNA interference technologies. This shift will include fusion of diagnostics with therapeutics, biotechnology with nanotechnology. 1Nanoderm Therapeutics Inc., 330 E. Liberty, LL Ann Arbor, MI 48104, USA

23

Biotechnology to Solve Some Local Health Problems: Arsenicosis, Molecular Characterization of Pathogens, Emergence of Multi Drug

Resistance and Development of Drugs for Multi Drug Resistant Pathogens

A.K. Azad Chowdhury1, Sheikh Zahir Raihan1, M. Asaduzzaman1, Nishat Nasreen1 and Nahid Akhter, A. K. M. Shahidur Rahman1, Mohammad Shawkat Ali1

& Golam Hasan Rabbani2, Mahbubur Rahman2, and Kaiser Ali Talukder2

1. Antioxidants (a recipe of vitamins, β-carotene, selenium) and Applephenon® in detoxifica-tion of Arsenic induced oxidative injury in rabbits Inorganic arsenic contamination of drinking water from various sources has been the cause of chronic arsenic poisoning in many countries including Bangladesh (1). Arsenic concentrations in drinking water in Bangladesh have been found to be higher than WHO recommended standards of .01mg/L (2,3). Recent surveys have shown that about 90% of the tube wells providing 97% of the drinking water were found to contain unacceptable levels of arsenic ranging from .05 to 3.0mg/L (2,3). Arsenic is known to cause severe adverse effects on human health including cancers of the skin and urinary bladder (4). It has been estimated that 75 million people are exposed to the risk of chronic arsenic poisoning; many of them showing early changes in skin and arsenic accumulation in scalp hair and finger nail.

The mechanisms of arsenic toxicity in man are poorly understood, although the involvement of oxidative stress, DNA damage, and genetic abnormalities has been suggested (5,6). During oxidative metabolism, arsenic produces free radicals such as superoxides anion (O2-) and hydrogen peroxide (H2O2) which induce lipid peroxidation and cell death. Similarly, nitric oxide (NO) or its toxic derivatives, peroxinitrites can damage cells by inhibiting mitochondrial respiration or interacting with reactive oxygen species (7). Many social and technical interventions are going on in Bangladesh to provide arsenic free drinking water to the people (2). But the efforts or intervention to alleviate sufferings of the millions of people who have already been exposed to varying degree of arsenic poisoning are very limited, if any. The studies conducted by this group are aimed at relieving the oxidative stress caused by the arsenic by using vitamins and natural products (mainly poly phenolic compounds) that have antioxidative properties. In the initial study, a receipe of Vitamins, A, C, E and beta carotene and selenium, and Applephenon(R) (a mixture of proanthocyanin and anthocyanin, a gift from Tomen Corporation, Japan) have been tested in the rabbit model of Arsenicosis. 2. Effect of black and green tea extracts (Ployphenols) on Arsenic induced oxidative injury in rabbits Sheikh Zahir Raihan, G. H. Rabbani, A.K. Azad Chowdhury Encouraged by the results of the Applephenon which is essentially a mixture of anthocyanins in relieving the arsenic induced oxidative stress in the rabbit model of arsenocosis [1], the authors became interested to test the effect of black and green tea extracts, which are known to have antioxidant properties due to their polyphenol constituents [2-4], in the model. The rabbit model of arsenicosis developed by the group in the previous study has been used to test the effect of Black and Green tea extracts in relieving the oxidative stress caused by arsenicosis.

24

Black tea (BT) and green tea (GT) extracts (polyphenols), each, when administered for the next 14 days caused a significant elevation of the depleted GSH level to 53.12 and 57.47% respectively in rabbit model of arsenicosis. On the contrary, in the placebo group (PL) the elevation was 26.59%. The BT and GT reduced the elevated TBARS level to 43.27%, 62.28% respectively whereas the corresponding reduction in PL group was 21.24%. The NOx levels were also reduced to 63.62%, 67.67%, and 58.94% in BT, GT and PL groups respectively. When arsenic and black tea extract are given concurrently in another group the results were even more pronounced. The polyphenols components of black and green teas were 27.69% and 29.71% of the dry weight of the total extracts respectively. These results indicate that arsenic induced toxicities in rabbits were significantly reversed by the black and green tea polyphenols. The greater activity of green tea compared to that of black tea correlates with slightly higher content of polyphenols in green tea. 3. Antibiotic Susceptibility and Molecular Characterization of Salmonella Paratyphi A Isolated in Bangladesh

K. A. Talukder ([email protected])1, M. Asaduzzaman2, Z. Islam1, M. Aslam1, A. S. Mondol1, I. J. Azmi1, M. A. Islam1 and A.K.A. Chowdhury2

Enteric fever, comprising both typhoid and paratyphoid fevers, continues to be a major public health problem even after the development and advent of newer antimicrobial drugs (1,2). Recent surveillance in Asia gives indication of increase in Paratyphoid fever and similar trends was also observed in the UK (2) Salmonella enterica serovar Paratyphi A is the second most common cause of enteric fever after S. Typhi in developing countries including Bangladesh. Till date there are no published data which can reflect the present status of the prevalence, susceptibility pattern and genetic properties of S. Paratyphi A strains in Bangladesh. The major objective of this study was to characterize Salmonella Paratyphi A strains using phenotypic and genotypic traits. A total of 524 strains of Salmonella Paratyphi A were isolated at Clinical Microbiology Laboratory from patients attending the Dhaka treatment center of ICDDR,B between January 1999 and June 2004. Of these, 31 strains were characterized extensively using serotyping, antibiotic resistance analysis, plasmid profile analysis and pulsed-field gel electrophoresis (PFGE) and DNA sequencing was performed to investigate the resistance mechanism against fluroquinolone antibiotics. Among the 31 strains, 32% (n=10) were resistant to nalidixic acid. Strains that showed reduced susceptibility to ciprofloxacin by disk diffusion method, had elevated MIC value (0.5µg/ml). Analysis for mutation in the gyrA and parC genes in the QRDR by sequencing revealed a point mutation in the gyrA gene at codon 83 (TCC to TTC), which substitutes phenylalanine for serine whereas no mutations were found in parC gene. Of 31 strains, 16 (52%) S. Paratyphi A strains harbored 35 MDa plasmid which was found to be non-conjugative. Pulse-field gel electrophoresis (PFGE) showed that most of the strains (83%) were clonal regardless of antimicrobial sensitivity patterns.

The study emphasizes further extensive characterization and epidemiological investigation of Salmonella Paratyphi A which will aid in undertaking strategy to combat antimicrobial resistance in the species and redefining the treatment strategy with conventional, effective antimicrobial agents in the face of the emergence of strains with reduced susceptibility to fluoroquinolone group of antibiotics. 4. Emergence of Optochin-resistant Streptococcus pneumoniae: Implications for Diagnosis and Management of Pneumococcal Diseases M. Rahman1, H. Rashid1, N. Nasrin2, K. Zaman1, N. Nahar1, and A.K.A. Chowdhury2 Acute respiratory tract (ARI) infections are now the principal causes of death in children under five years of age throughout the world including Bangladesh(1). Pneumonia accounts for up to

25

30% of all deaths in children less than 5 years of age (2,3). Those most commonly at risk for pneumococcal infection are children between 6 months and 4 years of age and adults over 60 years of age. New, safe effective vaccines have been developed, but the burden of pneumococcus in Bangladesh is unclear. Pneumonia is a frequent occurrence with incidence rates of at least 1 per 10 children per year in rural settings and >1 episode per child per year in urban settings. The global emergence of Streptococcus pneumoniae resistant to commonly used antimicrobial agents has complicated the management of pneumococcal diseases (4). The MIC results showed high rates of resistance to cotrimoxazole (77.9%) and tetracycline (46.3%)(5). Even in ideal situations pneumococci are difficult to isolate, and so the vast majority of pneumococcal infections are unrecognized, misdiagnosed or wrongly diagnosed with consequent treatment failure. The optochin susceptibility test remains the primary and, in some cases, the only method in clinical microbiology to differentiate S. pneumoniae from α-hemolytic viridans streptococci. However, emergence of optochin resistance in S. pneumoniae results in misidentification of pneumococci, lowering their isolation rate that jeopardizes the diagnosis, treatment and prevention of pneumococcal diseases. The present study was conducted in the ARI lab (LSD) of ICDDR,B and the main objective of the work was to observe the emergence of optochin-resistant S. pneumoniae. Fifteen Hundred nasopharyngeal swabs of mothers and infants enrolled in the study were subjected to the tests used to identify S. pneumoniae such as colony morphology, gram staining, optochin susceptibility and bile solubility tests and finally confirmed by lytA (autolysin) PCR. Antimicrobial susceptibility test and MIC value detection were also performed to see the emergence of drug-resistance pattern among the strains. In total, 111 optochin-resistant α-hemolytic streptococci strains were detected. When subjected to bile solubility test, 37 (33.3%) optochin-resistant, bile soluble S. pneumoniae were obtained, as other α-hemolytic-streptococci were not bile soluble. An S. pneumoniae-specific lytA gene PCR was positive in all 37 isolates confirming their identification. Thus, the optochin susceptibility test failed to differentiate S. pneumoniae from other streptococcal species, showing its decreasing sensitivity and specificity. Optochin-resistant S. pneumoniae had significant co-resistance to other antimicrobial agents (64.86% were resistant to penicillin, 78.37% to co-trimoxazole, 78.37% to ciprofloxacin, 45.95% to tetracycline and 27.03% to azithromycin/erythromycin) and multi-drug-resistance (resistant to ≥3 drugs, 64.86%).(6) Emergence of optochin-resistance in S. pneumoniae threatens the diagnosis, therapy and prevention of pneumococcal diseases because of failure to detect S. pneumoniae. For correct identification and, consequently, for correct treatment, α-hemolytic-streptococci with a typical colony morphology of S. pneumoniae but optochin resistant should be checked by bile solubility test or PCR for S. pneumoniae. Alternatively the bile solubility test should be routinely used for identifying S. pneumoniae. 5. Bioactivity guided search for antimicrobial principles against multi-drug resistant pathogens from Swietenia mahagoni A.K.M. Shahidur Rahmana, M. Shawkat Ali a, Hosne Ara Alia and A. K. Azad Chowdhurya Indiscriminate use of antibiotics in the developing world like Bangladesh has been the cause of the emergence of multidrug resistance pathogens. Many pathogens have developed resistance to many commonly used antimicrobials and thereby affecting the morbidity and mortality of the patients suffering from infective diseases. To cite few examples, the strains of Shigella, Salmonella, Streptococcus pneumoniae have developed resistance to commonly available antibiotics. The Shigella and Salmonella strains are resistant to the fluoroquinolones due to single to three point mutations in the gyrA and parC genes. Two of our group members are working on the topic involving Streptococcus pneumoniae Salmonella Paratyphi. In search of molecules with antimicrobial activity (also cytotoxic principles) Swietenia mahagoni, belonging to a family (Maliaceae) known to possess antibacterial activity and antiparasitic

26

activities has been subjected to bioactivity guided investigations. The methanolic extract of seeds of S. mahagoni was chromatographed on silica gel column, eluted with CH2Cl2 and CH3OH mixture in order of increasing polarity. The SMS1 (Rf = 0.25, silica gel-G: CHCl3), SMS2 (Rf = 0.50, silica gel-G: CHCl3). The structures of SMS1 and SMS2 were elucidated by spectra analyses (1H, 13C and DEPT135 NMR, Mass spectra) and CHN analyses. Table 1. Antibacterial activity of pure compounds SMS1, SMS2, SMLH1, SMLH3, SMLH8 isolated from Swietenia mahagoni against multi-drug resistant pathogens.

Test Bacteria SMS1 SMS2 SMLH1 SMLH2 SMLH3 SMLH8 Salmonella Paratyphi a 19 15 -- -- -- -- Shigella dysenteriae b 16 14 -- -- 13 E.coli c 15 16 17 -- 12 -- Staphylococcus aureus d -- 16 17 -- -- 11 Bacillus subtilis e 19-8 19 -- -- -- -- Streptococcus β-hemolyticus f 16 12 10 13 11 13

a resistant to Ciprofloxacin, b resistant to Ciprofloxacin, c resistant to Cotrimoxazole, d resistant to Amoxycillin, e resistant to Amoxycillin, f resistant ot Ciprofloxacin, Cotrimoxazole, Amoxycillin, Erythromycin and Doxycyline. SMS1, SMS2, SMLH1, SMLH2, SMLH3, SMLH8, each has 100 µgm/ml/disc The SMS1 and SMS2 spectra analysis by 1H, 13C and DEPT135 NMR analysis

SMS1: Proposed structure SMS2: Proposed structure

1Dept. of Clinical Pharmacy & Pharmacology, University of Dhaka, Dhaka-1000 2ICDDR,B: Centre for Health and Population Research, Dhaka Bangladesh

O

O

O

H

HO O

OHH

OH

OH8

30

1

3

5

9

Molecular Weight = 488.58, Exact Mass = 488 Molecular Formula = C27H36O8 Molecular Composition = C 66.38%, H

Molecular Weight = 584.67 Exact Mass = 584 Molecular Formula = C32H40O10 Molecular Composition = C 65.74%, H 6.90%, O 27.36%

O

O

O

HO O

OHH

O

OH

OO

8

30

1

3

5

9

27

Biosensors for Pesticide Detection in Fruits and Vegetables

M. Anwar Hossaina, Kagan Kermanb, Naoki Nagatanic, Akihiro Takeuchib, Teruko Yuhib, d,Tatsuro Endoe, , Yuzuru Takamurab and Eiichi Tamiyab

Widespread use of chemical pesticides for crop protection and other household uses have posed a grave threat to human health and environment. Day-to-day exposure to pesticides in fruits, vegetables, crops, drinking water, dairy products and common beverages can cause serious health hazards. The problem is steadily growing, especially, in the developing countries like Bangladesh, where monitoring system to detect residual pesticides in biological samples including food items is almost non-existent. We report on two new and versatile biosensors for easy detection of residual pesticides in biological samples.

In the first method the neurotoxic property of organophosphate pesticides due to the inhibition of acetylcholinesterase (AChE) was utilized to develop a disposable detection chips for easy and fast detection of the pesticides. An enzymatic assay involving acetylcholinesterase (AChE) was developed using a stable substrate specific to the enzymatic reaction product, thiocholine. The generated thiocholine reacted with 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) to produce 5′-mercapto-2′-nitrobenzoic acid, which was measured at 410 nm. Optimum pH, buffer types and concentrations, substrate concentrations and optimum conditions of the color reaction were investigated. The substrate specificity, test interferences were evaluated. Our simple detection system does not require any expensive instruments. Diazinon-oxon (DZN-oxon) could be detected by a visual color change from white to yellow on the surface of the chip. The total amount of time required for the detection of residual DZN-oxon in apple and orange juices was about an hour. This method provides excellent specificity, reproducibility, a wide measurement range and minimal interference from endogenous substances in juice matrices. Since the reagents are stable after preparation, our method would be useful for routine pesticide screening by non-professional end-users.

In a more sensitive system we monitored the recognition event between rabbit polyclonal antibody to parathion (parathion-pAb) and parathion by following their electrochemical current signals at a disposable pencil graphite electrode using differential pulse voltammetry. The current response was linear over a wide range between 6 ppb-2.60 ppm parathion. The applicability of the immunosensor to monitor parathion in distilled water and simulated well water, as well as in tomato juice, was demonstrated. We anticipate that the methods reported here will be readily applicable to the on-field detection of various pesticides since these will be suitable for miniaturization and mass fabrication. aDepartment of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh bSchool of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan cDepartment of Biotechnology and Applied Chemistry, Faculty of Engineering, Okayama University of Science, 1-1 Ridai-cho, Okayama 700-0005, Japan dJapan Science and Technology Agency (JST), Innovation Plaza Ishikawa, 2-13 Asahidai, Nomi City, Ishikawa 923-1211, Japan eDepartment of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan

28

Understanding Jute at the Molecular Level

Nadim Ashraf, Aleya Awal, Samiul Haque, Monira Obaid, Rokeya Sultana, Saaimatul Hoque, Shakinur Islam Mondal and Haseena Khan1

Very little molecular information of jute or its related species is available at the databank, and no real effort has been undertaken in the past to develop molecular markers to study its genetic variability. Hence we standardized the protocol for Random Amplification of Polymorphic DNA (RAPD) to allow fingerprinting of diverse jute germplasm and to identify DNA loci correlated with low temperature tolerance without any prior sequence information. Jute usually grows at a base temperature of 200 C, however four accessions have been identified from the seed bank, which are able to grow at a lower temperature of 160C. Classical genetics studies in our laboratory have indicated that this characteristic is mainly determined by a single and dominant DNA locus. An F2 population developed from a cross between two jute varieties, showing differential tolerance to low temperature, was screened with 50 RAPD primers. One of the polymorphic bands from RAPD, CT3-1200, was found to be strongly linked to the low temperature tolerance trait in the segregating F2 population of 100 individuals. This polymorphic band when cloned and sequenced revealed a 150bp region showing a strong homology (>90%) to the translated product of the terminal exon of an Arabidopsis’ expressed gene coding for low-density lipoprotein B like protein. With reverse transcriptase–PCR this exon has been shown to be expressed in jute. Both the local and global alignment of a 5′ RACE product confirmed that the gene might be a low density lipoprotein (LDLP). The SCAR (Sequence Characterized Amplified Region) primers were developed from the sequences which showed amplification for both parent genotypes. On sequencing the SCAR products, a single nucleotide polymorphic site was detected between the two genotypes. This gave rise to the potential to develop SNP assay linking with the low temperature tolerance in jute. Primer walking revealed another SNP at the RAPD primer binding site. Differential display RT-PCR has also identified differentially expressed genes in low temperature stressed jute seedlings. Bioinformatics analysis of expressed sequences revealed interesting findings where the evolutionary origin and putative function have been deduced with a particular sequence showing strong homology with a probable transmembrane protein of Agrobacterium tumefaciens. 1Plant Molecular Biology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh

29


Abul K M Ekramoddoullah1 Except for alien pests introduced into the environment, trees diseases and insect pests are natural components for forest ecosystems. Nevertheless, the damage they cause to forests can have severe economic impact. In British Columbia (on the Pacific Coast of Canada), this damage results in an estimated annual growth loss of 18%. Understanding host–pathogen interactions is important in managing yield loss and can aid in identification of disease-resistant trees. Molecular characterization of the genes and proteins that make up resistance and virulence phenotypes is critical to understand the function, evolution, and stability of a given conifer pathosystem. The study of interactions involving forest pathogens offers both challenges and opportunities. In this presentation, I will describe some of these issues from a perspective gained while working on the white pine–blister rust pathosystem. Several defence responsive proteins and their genes have been characterized through use of a proteomic approach. Some of these are identified as potential candidates for markers associated with disease resistance or susceptibility. Current research activities, future directions and the application of technologies to isolate and characterize resistance genes in white pine will be discussed. 1Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, Victoria, British Columbia, V8Z 1M5, Canada Email: [email protected]

30

Approaches to Production of Salt Tolerant Rice for Saline Costal Region of Bangladesh

Zeba I. Seraj1

Work is being conducted to identify DNA markers linked to salinity tolerance traits to aid in breeding programs for production of salt tolerant rice. The marker work has led to the identification of three regions within a 5 cM locus in rice chromosome 1, which is linked to salinity tolerance traits. These regions are 11.2-11.45, 12.0-12.3 and 12.6-12.7 million base pairs. Work for confirmation of three DNA markers within these regions is continuing using both mapping and breeding populations, so that these can be used in marker-assisted backcrossing programs or MAB for introgression of salt tolerance into farmer popular mega rice varieties. In a second approach, transgenic rice is being produced with the rice vacuolar Na/H antiporter gene (OsNHX1), which has previously been shown to confer salt tolerance in dicots, like tomato and Brassica. The rice has been found to be more tolerant than untransformed controls and the transcript expression to correlate with seedling tolerance in hydrponics. The transformed gene will now be backcrossed into farmer-popular varieties. Similar work by other workers have failed to produce rice which flowered under stress, probably due to poor expression of the gene by the CaMV 35S promoter. We have found the endogenous Pokkali promoter of the OsNHX1 gene to be much stronger than the CaMV 35S promoter. Work is progressing to hook the gene downstream of this promoter to achieve stronger expression. 1Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000

31

Improvement of Grain Legumes through Genetic Transformation

R. H. Sarker1, Rehana Hashem and M. I. Hoque In developing countries, grain legumes have gained much importance in view of the wide prevalence of protein malnutrition. In Bangladesh, a number of grain legumes are cultivated, which include lentil, chickpea, mungbean, blackgram and grasspea. However, the production of these crops is characterized by low yield potential. The diseases caused by different fungus are the main constraints of grain legume production. Conventional breeding methods did not produce desired varieties in the past due to the narrow genetic base and non-availability of resistance genes against the major fungal diseases in their respective germplasms. Under these circumstances, Agrobacterium-mediated genetic transformation can be one of the methods of choice to introduce gene/genes of interest into these crop varieties. Agrobacterium – mediated transformation compatible regeneration system was developed for the popular local varieties of lentil, chickpea and mungbean. In vitro organogenesis was found to suitable in achieving regeneration using various explants, The protocol for Agrobacterium – mediated transformation was developed through the utilization of marker genes like GUS (β - glucuronidase) and NPT II (neomycin phosphotransferease, conferring kanamycin resistance ) for all the varieties tested. The stable integration of the GUS and NPT II genes in the transformed plantlets was confirmed through histochemical GUS assay and PCR analysis. In the cases where regenerated shoots were failed to produce effective root system, in vitro grafting as well as in vitro flowering and seed setting experiments were conducted using the in vitro raised shoots. In vitro flowering and seed setting in lentil was found to be effective in recovering transgenic plants. Transformation experiments were further conducted using fungal resistance gene Ri-PGIP (raspberry polygalacturonase inhibitory protein). Integration of this gene in lentil varieties in T0 and T1 generations was confirmed through PCR analysis. A construct was further developed containing Ri-PGIP gene to perform marker free transformation. 1Department of Botany, University of Dhaka, Dhaka –1000, Bangladesh,

e-mail: [email protected]

32

Biotechnology for Rice and Solid Waste Decomposition 1M. Firoz Alam, 2M. Khalekuzzaman, 1,3S. Mahfuja Khatun,

4Swapan K. Datta and 3Philippe Herve Nowadays biotechnology has been proved as an important tool for sustainable crop improvement and keeps the environment friendly especially for agricultural soil. As rice is a globally important major cereal crop, which provides the staple diet for more than half of the total world’s population, therefore its yield potentiality should be sustained against different biotic and abiotic stress. Though rice is an important source of diet for human, the present form of rice does not fulfill the recommended dietary allowance. So, attention should be given to improve its nutritional quality. Among the biotic stress stem borer damage is a serious problem in rice, causing estimated losses of 10-30% of the total yield. Bacillus thuringiensis (Bt) produces characteristic crystalline insecticidal proteins which can disrupt the midgut cells of the insect pest. Bt toxins are highly specific and therefore are not toxic to the beneficial insects, birds and mammals including humans. The truncated chimeric Bt gene-CryIA(b) driven by constitutive and tissue-specific promoters was introduced into rice varieties. The presence and expression of the Bt gene in regenerated plants were confirmed by southern and western blot analysis. Inheritance of the transgene was confirmed in the successive generations. Insect bioassays showed an enhancement of resistance against the yellow stem borer. Like biotic factors, rice productivity is greatly affected by environmental stresses such as drought, high salt and freezing. Plants respond and adapt to these stresses to survive under stress conditions at the molecular and cellular levels as well as physiological and biochemical levels. So, environmental stresses can induce the expression of a large amount of genes. Among these are many transcription factors that regulate the expression of downstream genes by specifically binding to cis-elements or forming transcriptional complexes with other proteins. Expression of a variety of genes has been demonstrated to be induced by these stresses in a variety of plants. The dehydration-responsive element (DRE) with the core sequence A/GCCGAC was identified as a cis-acting promoter element in regulating gene expression in response to drought, high salt and cold stresses in Arabidopsis. It is important to analyze the DRE/DREB (CRT/CBF) regulation in rice to understand the molecular mechanisms of stress tolerance and produce monocots like rice with higher stress tolerance by gene transfer. It is known that the removal of the outer layers of rice grain by commercial milling considerably reduces the levels of micronutrients, including iron, because most of the iron is accumulated in the aleurone layer. Iron deficiency is the most common nutritional disorder in the world. Its effects on human health are severe. The most widely recognized strategies for reducing micronutrient malnutrition are supplementation (tables/capsule), food fortification, dietary diversification and disease reduction. For various reasons (such as continuous supplement supply, insufficient suitable food, lack of nutritional knowledge and high cost) none of these programs has been very successful in reducing the prevalence of iron deficiency anemia in developing countries. Therefore, genetic engineering or plant breeding is an alternative, more effective and sustainable approach for the enrichment of food staples (such as rice) to overcome iron-deficiency anemia, especially in developing countries. Ferritin is an iron-storage protein found in plant, animals and bacteria. To increase iron storage in rice, the soybean ferritin gene driven by an endosperm specific glutelin promoter (GluB-1) was introduced into a Bangladeshi rice cultivar, BRRI Dhan 29 (BR 29). Analysis demonstrated integration, inheritance and expression of the ferritin gene up to the T3 generation. The iron content in seeds was estimated by using the ICP (Inductively coupled Argon plasma) spectrometer. All transgenic plants accumulated higher level of iron in the grain, with as much as 9.2 mg/kg verses the control (3.8 mg/kg). A histochemical relation of the thin microtome section revealed the presence of iron in the endosperm cells of the transgenic grain. This finding suggests that homozygous rice lines with enhanced iron content developed by genetic engineering could help overcome iron deficiency in developing countries.

33

Any crops with high genetic potentiality to produce excepted yield may fail to do so due to soil problems like having low organic matters content or toxified with harmful chemical or heavy metals. On the other hand fertilizers are used to provide the minerals lacking in some soil, and to replace the minerals remove from the soil by crops as they grow. Many farmers rely on concentrated fertilizers that are rapidly absorbed by plants. These fertilizers produced quick growth but at the same time may kill important soil organism such as earthworm and bacteria. Farmers use manure, compost (a mixture of decaying organic matter that is rich in beneficial soil microorganism) and other natural materials as fertilizers that nourished soil organism which in turn slowly and steadily make minerals available to plants and eventually helps to reduce dependence on the chemical fertilizers. Everyday huge quantities of municipal garbage are generated in all cities. The safe disposal of the garbage is a major complex problem that can affect the year, land, water and environment. Therefore, proper operation, maintenance and appropriate technology are essential to overcome this serious problem by proper utilization of garbage. Conversion of garbage in to valuable organic compost seems to be an immediate solution of the problems. Trichoderma is notably capable of producing various polysaccharides-degrading enzymes, which enable it to grow on organic (domestic) waste. It has been reported that some Trichoderma spp are widely used to produce various industrially important enzymes, thus proving potential use of Trichoderma for bioconversion of solid organic waste. Besides this Trichoderma harzianum also has the ability to degrade organochlorine pesticides, such as DDT, dieldrin, endosulphan, pentachloronitrobenzene and, pentachlorophenol, and hence has potential application for bioremediation. Trichoderma ssp have been known to suppress many soil-born fungi and nematode diseases under greenhouse and field conditions. Trichoderma harzianum, Trichoderma hamatum has been found to antagonize fungal plant pathogens and parasitic nematodes. It is also known that Trichoderma is able to increase the rate of plant growth and regulation. Recent research indicates that Trichoderma can reduce the nitrogen requirement of many crops such as corn up to about 40%. So use of this organism may provide a useful method to retain high agricultural productivity. Similarly, Effective bacterial strains (Cellulomonas, Clostridium, Bacillus, Xanthomonas, Pseudomonas and Streptococcus) are unknown to us, which are very active for bioconversion of solid organic waste. So, research should be taken to keep our agricultural soil environmentally friendly by using effective soil microorganisms like fungi and bacteria. Trichoderma harzianum, Trichoderma pseudokoningii and Trichoderma virens were isolated and identified from the local garbage heap. Among the three strains Trichoderma harzianum was found most effective on conversion of organic solid waste. Highest weight losses (32%) were found after 30 days of treatment with gradual changing of colour from greenish to deep greenish colour with no bad small. Comparing between using of pellets and spore suspension, pellets were found more effective for decomposition. Similar findings were also observed in case of using studied bacteria for solid waste decomposition. So, use of fungi and bacteria not only effective for solid waste decomposition but also effective for bioremediation.

1. Department of Botany, University of Rajshahi, Rajshahi, Bangladesh 2. Department of Genetic engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh 3. International Rice Research Institute, Philippines 4. Department of Botany, University of Calcutta, Kolkata, India.

34

Germplasm Center (GPC)-The Largest Fruit Genetic Resources for Biotechnological Research

1M. A. Rahim, H. R. M. Masud Anwar, M. S. Alam, M. A. Kabir, B. C. Sarker, M. S. Bari,

N. Naher, F. Islam, D. A. N. Majumder

The fruit tree improvement program developed the largest fruit repository in Bangladesh. All sorts of research and development of on fruit are going on. About 60 MS and 12 Ph.D. students from different Universities doing their research here in GPC. This genetics resources can largely be used in biotechnological works.

1Fruit Tree Improvement Program (FTIP), Department of Horticulture, Bangladesh Agricultural University, Mymenisngh-2202

35

Morpho-molecular Characterization of Plant Varieties of Bangladesh for Plant Variety Protection1

Lutfur Rahman, Rezwan Mollah, Sayeda Sulata, Mohammad Nazrul Islam, Nesar Uddin Ahmed, Md. Shefatur Rahman, Md. Nazim-ud-Dowla , M.Shah-e-Alam, and M.S.Alam2

Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh,

This titled paper is a result of research studies on crop varieties of Bangladesh aimed at characterization and documentation of released and registered varieties of all crops. This was done in order to identify each of the variety with both morphological and molecular characters. In recent years such study has become essential to establish sovereign rights of the country over the varieties, rights of the breeders and farmers who have developed or maintained the variety to help organized protection of the genetic resource by the Government of Bangladesh. The project is financed by the Seed Industry Development project of the Ministry of Agriculture, Government of the People’s Republic of Bangladesh under the Assistance of the DANIDA. The study covered a period of 15 months from April 2005 to June 2006 and was conducted at the Department of Genetics and Plant Breeding, Bangladesh Agricultural University. A team of scientists was formed and worked on the crop varieties. The study completed the genetic finger printing and identification of the distinct morphological characters of 157 varieties of 20 crop species. The study results have been recorded through qualitative and quantitative data using the crop descriptors of the IBPGR as approved by the government of Bangladesh. In case of the DNA finger printing, the SSR and RAPD markers were used. Out of 20 crop species/varieties Jute (both species), Mesta, Kenaf, Sesame, Sunflower, Safflower and Linseed have been tested through RAPD markers. RAPD was used for these species because the team did not find appropriate identified primers of SSR for those crops from world literature. The genetic diversity available in different varieties of a species has been estimated and clusters determined. In rice alone, modern and traditional varieties have been studied separately. In case of traditional varieties, except one all the other 14 varieties were identified distinctly from one another with specific primers. This means that the varieties could be identified using SSR site specific primers of rice. While in case of the modern varieties 5 of the 19 varieties could not be differentiated using the same three primers. This indicates that the new primers having capacity to identify other loci are required to differentiate those five verities from one another. In cases of other crop species like wheat, barley, maize, potato, cotton, sugarcane, rapeseed, soybean and groundnut, the SSR primers used could differentiate one variety from the other indicating that the variety specific primers have been identified. It was interesting to note that in maize, which is a cross pollinated crop, there have been a number of heterozygous loci mapped by the primers. In cases of three species of Brassica oil crops used in the study similar variation was observed. The potato and sugarcane have shown a further situation where the involvements of polyploidy in their development have become distinct through the use of SSR primers. In overall conditions, the study clearly indicates the possibility of identifying and differentiating varieties with specific primers of SSR. The varieties thus identified as distinct from others are also photographed for specific morphological trait(s) of distinction, if any, and the information on quantitative traits is also used for distinction. ________________________________________________________________________

1A project of the Seed Industry Development, Ministry of Agriculture, GoB, Financed by the DANIDA 2 Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh. This extended abstract is part of the published abstract of the special article in 33(2) of the BJAS for record of the 157 crop varieties of 20 species to be protected by the Government of Bangladesh.

36

Molecular Characterization of Landraces and Wild relatives of Some Important Vegetables and Fruits of Bangladesh

M.G. Rabbani1, M. A. Rahman1 and E.J. Garvey2

Under a USDA funded project at Bangladesh Agricultural University, Mymensingh; more than 2000 landraces and wildrelatives of some important vegetables and fruits of Bangladesh was collected and characterized. For characterization, both morphological traits and molecular markers (isozymes and RAPD) were used. Genetic diversity among the germplasm of the collected species was estimated following Mahalanobis (1932) D2 analysis using the morphological traits and molecular markers. Cluster analysis using morphological traits and molecular markers revealed that in majority of the cases, the grouping of germplasm based on morphological traits did not match with grouping based on isozymes and RAPD markers. Based on morphological traits and molecular markers, the collected germplasm were also tested for genetic divergence using D2 techniques. Results revealed that the germplasm collected from the same places were grouped into different clusters indicating that there is no relationship between genetic divergence and geographical distribution of the germplasm. Estimates of Nei’s (1973) gene diversity and Shannon (1968) information index across all loci supported the existence of high level of genetic variation among the collected germplasm of different vegetables and fruits. The detail results on characterization of the landraces and wildrelatives of different vegetables and fruits of Bangladesh based on morphological traits and molecular markers will be presented. Key words: Landraces, Wildrelatives, Genetic diversity, Molecular characterization, Isozymes, RAPD

1 Professor, Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202 2Plant Exchange Office, USDA/ARS, BARC-West, Beltsville, Maryland MD 20705, USA Name of corresponding author: Professor Dr. Md. Golam Rabbani Email: [email protected] [email protected] Cell: 01711885790 Fax: 880-91-55810

37

Generation of Bioengineered Rapeseed for Salt Tolerance

Lutful Hassan and S.K. Talukder Agricultural productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. Overexpression of a vacuolar Na+/H+ antiport from Arabidopsis thaliana that promotes sustained growth and development in soil watered with upto 200 millimolar sodium chloride. This salinity tolerance was correlated with higher-than-normal levels of AtNHX1 transcripts, protein, and vacuolar Na+/H+ (sodium/proton) antiport activity. These results demonstrate the feasibility of engineering salt tolerance in plants. Keeping this view in mind, we attempted the generation of salt tolerant rapeseed (Brassica campestris and Brassica napus) varieties in Bangladesh by the application of genetic transformation. To achieve the goal, the salt tolerant gene AtNHX1 from Arabidopsis has been inserted in Rapeseed varieties. Agrobacterium rhizogenes strain LBA 9402 was used for the production of hairy roots. For co-transformation experiments the strain LBA 9402 with the binary vector pBIN19 containing the p35S GUS INT gene was used. For plant regeneration 0.5 mm sections of root material were excised and treated with a liquid callus-inducing medium (C23γ) for three days. After that they were placed on N5 medium with antibiotics. The GUS staining was carried out according to Jefferson et al. (1987). Agrobacterium tumefaciens strains: I) GV3101 with the vir plasmid pMP90, II) the strain C58C1 ATHV with the vir-plasmid pTiBo542, a strain similar to EHA101 were used. The selectable marker gene, nptII (neomycin phosphotransferase) was used. The reporter gene β-Glucuronidase under control of the Ubi and the 35S-Promotor and with an Intron was used. Stem segments proved to be the best explant. Shoot regeneration in Agrobacterium rhizogenes transformation experiments was not successful. Regeneration from Agrobacterium tumefaciens mediated transformation proved to be successful. Insertion of salt tolerant genes (AtNHX1) from Arabidopsis thaliana in the popular varieties of Brassica genotypes is in progress. The transformed rapeseed varieties will be used by the farmers of the coastal wetland of Bangladesh.

Key words: Genetic transformation, Agrobacterium, Brassica campestris, Brassica napus, salt tolerance Name of corresponding author: Lutful Hassan Biotechnology & Genetic Engineering Laboratory, Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh-02202, Bangladesh E-mail: [email protected]; Fax: 091 55810

38

Plant Defensin For Improving Plant Resistance

Aparna Islam1 and V. S. Reddy2

Plants are exposed to enormous numbers of pathogens. But the appearances of diseases are rare. This is due to the presence of defense mechanisms. Various antifungal compounds protect seed, which is a good source of nutrition for pathogens while the source of next generation for plant. These antifungal compounds secreted out during germination and protect the young plant from fungal attach. In the present study, screening of antifungal agent was performed against Pythium aphanidermatum. Out of six legumes seeds screened, chickpea (Cicer arietinum, L.) was found to have inhibitory effect on the test fungus. Isolation and purification was performed in a simple two-step method. Sequence analysis showed that it is a “Plant Defensin” peptide and it was termed Ca-AFP. Further characterization revealed that this peptide is highly stable at extreme pH (2-10) and temperature. Moreover, it is effective towards broad range of phytopathogenic fungi. To assess the antifungal activity of Ca-AFP in heterologous system, transgenic tobacco plants were raised by introducing Ca-AFP gene using Agrobacterium-mediated transformation. Variation in expression of transgenes was observed within the raised transgenic plants but no morphological difference was observed in compare to non-transformed control. Moreover, during fungal bioassay these plants demonstrated effective inhibition towards susceptible fungi ranging from complete restriction of the inoculated fungi to reduced fungal growth. Effectiveness of this defensin gene in heterologous system opens up an opportunity to employ this gene into many of our important crop plants to improve disease resistance thus ensuring production and food security.

1 Department of Mathematics and Natural Sciences, BRAC University, Dhaka, Bangladesh.

2 ICGEB, New Delhi, India.

39

Phylogenetic Relationship of Phytophthora citrophthora Isolates based on rDNA Internal Transcribed Spacer Sequence Analysis

A.F.M. Jamal Uddin1, M. Senda2, S. Uematsu3, and K. Kageyama4

The global significance of the genus Phytophthora as important crop pathogens has been recognized. P. citrophthora is one of the most important pathogens in Citrus species. The pathogen is involved in trunk and crown canker of apple, pear, peach, plum and other woody Rosaceae, avocado, honey-locust and walnut; and damping-off of a large variety of nursery seedlings of tomato and conifers as well as Citrus species. Traditional identification method in Phytophthora species requires time, labors and skills. And the complex taxonomical treatments sometimes make miss-identification. The internal transcribed spacer of ribosomal DNA (rDNA-ITS region) is commonly used for taxonomy and identification in Phytophthora species. The rDNA-ITS region of P. citrophthora isolates was amplified using the PCR with universal primers, ITS1 and ITS4, and sequenced. Nineteen isolates from P. citrophthora from different locations of Japan were examined and compared with the sequences of the other isolates of the species and the genetically related species obtained from DNA database. The consensus tree of the rDNA-ITS region, constructed by maximum parsimony with a bootstrap 50% majority rule, reveled that the phylogenetic relationship among the isolates observed on the rDNA-ITS region tree did not exhibit any consistent clusters depending on the host range, geographical distribution and the isolation time, although there are some intra-species and intra-isolate variation. The result suggests that P. citrophthora will be a distinct species, although the species consists of the isolates which have different host and geographical origins. Key words: Phylogeny, ITS region, rDNA, PCR and intra-specific variation 1 Sher-e- Bangle Agricultural University, Dhaka, and Post Doctoral Researcher, River Basin Research Center, Gifu University, Japan 2 Reserch Associate, River Basin Research Center, Gifu University, Japan

3 Head, Plant Pathology Division, Southern Prefectural Horticultural Institute, Chiba, Japan

4 Professor, River Basin Research Center, Gifu University, Japan Corresponding author: Abul Faiz Md. Jamal Uddin PhD Email: [email protected] Fax: 81-58-293-2063

40

A Classical and Molecular Analysis: Are Genes for Salinity Tolerance at Seedling and Reproductive Stages in

Rice the Same or Different?

M.M. Islam1 and G.B. Gregorio2 Microsatellite or simple sequence repeats (SSR) was the marker-aided selection (MAS) technique utilized to determine salinity tolerance in rice. Tagging genes for salinity tolerance in rice is an important step in identifying candidate markers flanking the genes. The genes for salinity tolerance in Pokkali were mapped utilizing the F8 recombinant inbred lines (RILs) of IR29. Phenotyping of 80 RILs and their parents (IR29/Pokkali) at the seedling stage was done using salinized (EC 12 dS/m) culture solution under controlled conditions at the IRRI Phytotron and at the reproductive stage using salinized (EC 5 dS/m) water at the IRRI greenhouse. The 12-linkage group map, which was constructed using 93 microsatellite markers, collectively covered 2433.3 cM at an average interval between markers of 25.39 cM. Quantitative trait loci (QTL) mapping determined the positions and effects of QTL for yield components and traits associated with salt tolerance in rice. Single marker analysis and interval mapping were combined in the QTL analysis procedures and all approaches showed similar QTL detection results. QTLs associated with salinity tolerance at the reproductive and seedling stages were tagged on chromosomes 1, 3, 4, 7 and 9. Two QTLs were identified for percent reduction of filled grain weight (RFGWT), four QTLs for percent reduction of biomass weight (RBWT), three QTLs for percent reduction of total biomass weight (RTBWT), and three QTLs for seedling stage tolerance. Common QTLs for the three quantitative traits (RFGWT, RBWT, and RTBWT) for salinity tolerance were observed in chromosomes 7 and 9 at the reproductive stage. Common QTLs for RBWT and RTBWT were also detected in chromosomes 4, 7, and 9. The proportions of phenotypic variations explained by each QTL ranged from 17.23 to 21.25% for RFGWT, 16.53-39.07% for RBWT, 17.62-24.74% for RTBWT, and 16.24-28.6% for seedling tolerance with higher logarithm of odds (LOD) value of >3.0. The QTLs detected among the traits (RFGWT, RBWT and RTBWT) at the reproductive stage and the salt stress rating at seedling stage did not share the same map locations, suggesting that the genes controlling salinity tolerance at the seedling and reproductive stages of rice are different. Key words: Mapping, QTLs, RILs, Salinity, Rice.

1Biotechnology Lab., Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, P.O. Box 4, Mymensingh 2200, Bangladesh 2Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines Name of corresponding author: Dr. M.M. Islam Email: [email protected], Fax: +88-091-54091, 54046

41

Application of Molecular Markers to Rice Breeding Populations and Haplotype Diversity for Salinity Tolerance in Chromosome 1

M.R. Islam1,2, G.B Gregorio1, B.C.Y. Collard1, E. Tumimbang-Raiz1, D.L. Adorada1, R.D.

Mendoza1, M.A. Salam2, and L. Hassan3

SalTol is a major quantitative trait locus (QTL) for salinity tolerance in rice. Three F2 breeding populations derived from the crosses viz. ‘BRRI dhan40’ (moderately tolerant)/ ‘IR61920-3B-22-2-1’ (highly tolerant); ‘BRRI dhan28’ (highly sensitive)/ ‘IR50184-3B-18-2B-1’ (moderately tolerant); and ‘Kajalsail’ (tolerant)/ ‘IR52713-2B-8-2B-1-2’ (tolerant) were used to studies. 20 SSR and two EST markers were used for the targeted mapping of the chromosomal region containing SalTol (49.6 to 87.1 cM) on chromosome 1. There was a good parallel of among the linkage maps of the three populations to the previous QTL map that identified SalTol. SalTol QTL was detected only in population derived from ‘BRRI dhan40’/ ‘IR61920-3B-22-2-1’ cross. The SSR marker RM8094 was the most tightly-linked marker (P<0.001) comparison to four other markers, RM1287, RM3412, RM493 and CP03970, which were also significantly associated with salinity tolerance (P<0.05). A F3 population of this cross was used to reconfirm these results and similar result was observed as in the F2 population. It was interesting to identify SalTol in this population through the tolerant parent was un related to the tolerant parent used for the original mapping studies. No QTLs were detected at the SalTol locus in any of the other two populations. This indicates that the SalTol QTL could only be effective in specific populations. To determine the usefulness of specific SSR markers associated with major QTL for salinity tolerance designated as ‘SalTol’, a collection of 115 diverse rice genotypes were phenotyped and genotyped using tightly-linked DNA markers viz. five SSR RM1287, RM8094, RM3412, RM493, RM140 and two EST CP6224, CP03970 on chromosome 1. Among the seven markers, RM8094 produced the highest number of alleles (15) followed by RM1287, RM3412 and RM493 (10). The polymorphic information content (PIC) values ranged from 0.54 to 0.89 with highest for RM8094, followed by RM493 and RM3412 (0.81), RM1287 and RM140 (0.77). It suggested that RM8094 markers could be useful for discriminating tolerant and susceptible may be successfully used for marker assisted selection supplementing the conventional breeding. Out of 115 genotypes studied seven haplotypes were identified with reference haplotype of IR66946-3R-178-1-1 (FL478), one of the most widely-used tolerant parents. Four genotypes had the same haplotype as of FL478. Of the seven different Pokkali seed accessions from different sources, Pokkali-1 (IRGC8948) was the actual source contributed the SalTol region in chromosome 1 segment of FL478. Genotypes from the haplotypes 1, 2, and 5 could be important for selecting alternative tolerant parents. Keyword: Salinity tolerance, QTL, haplotype diversity, rice

1Plant Breeding, Genetics and Biotechnology Division, International Rice research Institute DAPO Box 7777 Metro Manila,

Philippines 2Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh 3Genetics and Plant Breeding Department, Bangladesh Agricultural University, Mymensingh, Bangladesh

42

Salt Tolerant Rice Development through Genetic Engineering

Shamsul H. Prodhan1,2, K. Nakao,2 K. Nagamiya2, T. Motohashi2, S. Jesmin3., A. Komamine4 and H. Morishima2.

In an attempt to improve the salt tolerance of rice, we introduced katE, a catalase gene of Escherichia coli, into the indica rice cultivar Kasalath. Plant morphological variations and survival of plants under different salt water concentrations were checked. Transformation was carried out by using Agrobacterium tumefaciens strain EHA101 harboring a binary vector pIES6/Hm/katE which contains genes for catalase katE, hygromycine resistance gene HPT and kanamycine resistance gene NPTII in the T-DNA region. With the inclusion of acetosyringon higher amount of transgenic cells and regenerated plants were obtained. Transformation was confirmed with PCR and and Southern hybridization analysis. In this research we treated the transgenic plants in three different growth stages. Three days aged plants in 100 mM concentration survived up to 15 days and in 250 mM concentration up to 7 days. Four week aged plants in 100 mM concentration formed immature inflorescence and in 250 mM concentration survived up to 20 days. Six to seven week aged plants were able to grow for more than 20 days in the presence of 250 mM sodium chloride and produced seeds for more than 3 months in the presence of 100 mM sodium chloride. On the contrary, non-transgenic rice plants could not survive for 10 days even in the presence of 50 mM sodium chloride. Catalase activity was 1.5 – 2.5 fold higher in T1 plants than non-transgenic rice plants. Introduction of a single trait significantly improved the salt tolerance of indica rice plants. This result indicates that over expression of catalase improved salt tolerance efficiently in recalcitrant rice plant and the transgenic rice plant reported here is one of the highest salt tolerant indica rice plant which have been reported so far. Keywords: Rice (Oryza sativa L.), environmental stress, salt tolerance, transformation, katE, E. coli., restriction enzyme, Agrobacterium. 1Laboratory of Plant Genetic Engineering, Institute of Life and Environmental Sciences, University of Tsukuba, 1-1-1Tennodai, Tsukuba city, Ibaraki prefecture 305-8577, Japan.

2Laboratory of Genetics and Plant Breeding, Department of Agricultural Science, Tokyo University of Agriculture, Japan 3Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan 4The Research Institute of Evolutionary Biology. 2-4-28, Kamiyoga, Setagaya-ku,Tokyo, 158-0098, Japan. Corresponding author: Dr. Md. Shamsul Haque Prodhan E-mail address: [email protected] Fax: 88- 07326-63888

43

Generation of Monoclonal Antibody for Prostaglandin D2 Using the Stable, Isosteric Analogue as an Hapten Mimic and its Application

M. A. Mazid1, M. A. Rashid1, A. A. Chowdhury1, 2, K. Nishimura2,

M. Jisaka2, T. Nagaya2, and K. Yokota2 Prostaglandin (PG) D2 is a well-known cyclooxygenase metabolite of arachidonic acid, having a variety of biological activities. To determine the production of PGD2 in response to external signaling molecules, immunological methods would be convenient and useful. However, PGD2 is unstable under the physiological conditions, so that it has been difficult to get a specific antibody for the parent PGD2. To get an antibody specifically recognizing PGD2, we attempted to prepare monoclonal antibodies for 11-deoxy-11-methylene PGD2, a novel, chemically stable, isosteric analogue of PGD2. We were successful to get a cloned hybridoma cell line secreting a monoclonal antibody reacting against the parent PGD2. To develop the enzyme-linked immunosorbent assay (ELISA) for PGD2, the immobilized antigen using the stable PGD2 derivative was immunoreacted in a competitive manner with the monoclonal antibody in presence of free PGD2. The monoclonal antibody showed a cross-reaction of 36.8% for PGD2 as compared with the corresponding derivative. The optimization of the assay provided a sensitive calibration curve for PGD2 from 0.32 pg to 0.18 ng with a value of 7.6 pg at 50% displacement. According to the calibration curve for PGD2, PGF2� and PGE2 showed cross-reactivities of 5.9% and 3.7%, respectively, whereas those of other related prostanoids were less than 1%. The developed assay method was useful for applying to the direct determination of PGD2 in the culture medium of mouse 3T3-L1 adipocytes. Key words: Prostaglandin D2; 11-Deoxy-11-methylene-PGD2; Monoclonal antibody; Enzyme-linked immunosorbent assay (ELISA). 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh. 2Department of Life Science and Biotechnology, Shimane University, Matsue, Shimane 690-8504, Japan Name of the corresponding author: Dr. M. A. Mazid E-mail: [email protected] Fax: 88-02-8612069

44

Molecular Diagnostic Tests for Enteric Protozoan Parasites: Recent Developments

Rashidul Haque1

The etiological agents of diarrhea include viruses, bacteria and parasites. Among parasites Entamoeba histolytica, Giardia lamblia and Cryptosporidium spp., are considered to be the most common enteric protozoan parasites and associated with diarrheal diseases Amebic liver abscess is also due to infection with the enteric protozoan parasite Entamoeba histolytica. This parasite has recently been separated using modern diagnostic techniques from the nonpathogenic parasite E. dispar, which is more common and identical in appearance to E. histolytica. The WHO, estimates that approximately 50 million people worldwide suffer from invasive amebic infection each year, resulting in 40 to 100 thousand deaths annually. Giardia lamblia (synonyms: Giardia intestinalis and Giardia duodenalis) is the most common protozoan infection of the intestinal tract worldwide. G. lamblia is considered as one of the main non-viral causes of diarrhea in developed countries. Cryptosporidiosis is a frequent cause of diarrheal disease in humans. In developing countries, Crryptosporidium spp., infections occur mostly in children younger than 5 years, with a peak in children under 2 years of age. In immunodeficient humans, especially individuals with HIV/AIDS, cryptosporidiosis can be associated with chronic, potentially life-threatening diarrhea.

Diagnosis of these enteric protozoan parasites has historically been performed by microscopy. Microscopy is neither a sensitive nor a specific test for detection of these enteric protozoan parasites. Moreover, microscopy is unable to distinguish invasive parasite E. histolytica from the commensal parasite such as E. dispar and E. moshkovskii. Microscopy is also unable to detect these parasites at species level or their genotypes. Over the last decade a lot of work has been carried out to develop molecular diagnostic tests for these enteric protozoan parasites. In our lab we developed antigen detection test and PCR tests for specific detection of E. histolytica in collaboration with the University of Virginia, USA. We have also established and evaluated antigen detection tests, PCR tests for detection of and Cryptosporidium spp., and Giardia intestinalis. Here, we present some of the results that we have obtained in our recent studies.

1Parasitology Laboratory, Laboratory Sciences Division, ICDDR,B

45

Screening Endemicity for Lymphatic Filariasis (LF) and Development of Indigenous Molecular Diagnostics

Running Title- LF Endemicity & Indigenous Molecular Diagnostics

Apala Farhat Naved1 Lymphatic filariasis (LF) creates burning socio-economic problems in Bangladesh. Especially affected are the northern districts. Patients and their attendants in Rangpur Medical College Hospital (RMCH) were screened to select LF endemic study spots. A total of 205 nocturnal blood samples were examined by microscopy and 6 of the volunteers were found microfilaremic. Hydrocele found in 3 of the male volunteers. Bancroftian adult worm antigen found in 37 of the volunteers and 9 of them were PCR positive. All the microfilaremics were PCR positive and all the PCR positives were bancroftian antigen positives. Localities of the microfilaremics indicated presence of endemic areas in Rajendrapur Union and the villages, Radhakrishnapur, Kamdevpur and Gopinathpur were studied as endemic spots there. Among 192 of the volunteers studied in Rajendrapur, 13.9% and 1.8% of the males found with hydrocele and elephantiasis respectively. Elephantiasis was also present in 5.95% female volunteers. Altogether 11.46% of the study population was symptomatic. Microfilaremics were 10.18% of the males. All of the microfilaremics were bancroftian PCR positives and all the PCR positives (53) were found to be bancroftian antigen positive. PCR based molecular diagnostics developed using (1) nocturnal blood samples, (2) diurnal blood samples, (3) blood (nocturnal) stained filter paper, (4) diurnal sputum samples, (5) diurnal urine samples, & (6) mosquito samples. Previously established and available immunodiagnostic tools were also used to detect endemicity and infections. It was found that the PCR positive individuals with nocturnal blood were found also to be PCR positive with the diurnal blood, sputum and urine samples. All the infections were found to be bancroftian through microscopy, immunodiagnosis, PCR based assays of both human and mosquito samples, and DNA sequencing of the species specific PCR products. It’s the first confirmation at molecular level that Cx. quinquefasciatus is the LF vector in Bangladesh. PCR based assays are also developed first time in Bangladesh to detect (1) endemicity through quick mosquito screening and (2) LF infection in nocturnal and diurnal blood, sputum and urine samples at sub-clinical level, which is essential to detect present infection for treatment before occurrence of the irreversible disabling deformities of the body.

Key words: Lymphatic filariasis (LF), microfilaremic, Wuchereria bancrofti, Culex quinquefasciatus, polymerase chain reaction (PCR).

1Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh.

46

Evaluation of Antibodies in Lymphocyte Supernatant- New Hope for Diagnosis of Pediatric TB.

Rubhana Raqib1, Dinesh Mondal1, Anwarul karim2, Fahima Chowdhury1, Sultan

Ahmed1, Sanaul Bashar3, Stephen Luby1, Jan Andersson4, David Sack1. Diagnosis of tuberculosis (TB) in children presents challenges because commonly accepted clinical diagnostic criteria may be difficult to fulfill. We evaluated the TB diagnostic performance of antibodies in lymphocyte supernatant (ALS) assay in children suspected of having TB that was earlier found to be useful in the diagnosis of pulmonary TB in adults. A cross-sectional study was conducted in 56 children (age range 18-167 months) presenting to a hospital with presumptive diagnosis of TB. In the absence of an acceptable gold standard test, children were initially categorized as "radiologically certain TB," "probable TB” with radiological signs and good clinical response to anti-TB treatment, and "non-TB" who did not meet clinical diagnostic criteria. About 9% of the patients were diagnosed as cultured confirmed TB and 66% as probable TB. Patients with confirmed and probable TB had significantly higher TB-specific antibody titers at enrollment compared to the healthy control children and non-TB children (P<0.001). The sensitivity and specificity of the ALS method were found to be 86% and 90% respectively at a cut-off value of 0.3. The ALS titers declined from enrollment through 6 months following anti-TB therapy (p=0.001). Thus, the ALS method may be used as a potential aid for improved diagnosis of pediatric TB.

1. ICDDR,B: Centre for Health and Population Research, Bangladesh; 2. Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka; 3. Nari Maitree, Urban Primary Health Care Project PA-5, Dhaka City Corporation; 4. Center for Infectious Medicine, Karolinska Institutet, Huddinge University Hospital, Sweden.

47

Effects of Iron and Zinc Supplementation during Pregnancy on Neonatal Iron Status in Rats.

Mohammad Bakhtiar Hossain1,2, Shannon L Kelleher2, and Bo L Lonnerdal2.

Iron (Fe) and zinc (Zn) are necessary for optimal growth and development. Fe plays a key role in the transport of oxygen and development of cognitive function. Zn is associated with more than 300 enzymes by participating in their structure and or in their catalytic or regulatory actions. In a developing country like Bangladesh Fe and Zn deficiencies are common. Pregnant women and growing children are most vulnerable to Fe and Zn deficiency. Fe and Zn deficiencies during pregnancy may result in neonatal Fe and Zn deficiency. To combat this problem, co-supplementation of Fe and Zn during pregnancy is often recommended. However, supplementation with high molar ratio of one mineral has been shown to inhibit the absorption of the other. Although co-supplementatAion of these micronutrients may interact in pregnant woman, effects of maternal Fe and/or Zn supplementation on neonatal Fe status, Fe absorption and Fe transporters are unknown. Rats were fed a low Fe and Zn diet (12 µg/g Fe; 10 µg/g Zn) for 1 mo, then bred. Pregnant rats were supplemented 3 times/wk with vehicle (UnS), Fe (FeS; 9.0 mg), Zn (ZnS; 1.5 mg) or Fe and Zn (FeZnS). At weaning, Fe status, 59Fe absorption, and intestinal Fe transporter expression were determined in the pups. Pups from dams given FeS and FeZnS had higher hemoglobin (91 ± 4 and 80 ± 3 g/L, respectively) compared to UnS (56 ± 11 g/L) and ZnS (63 ± 5 g/L). Liver Fe was higher in FeS pups (31.1 ± 9.8 µg/g) compared to UnS, ZnS and FeZnS (20.2 ± 3.6, 20.1 ± 3.7, 22.2 ± 4.9 µg/g, respectively). Iron absorption was higher in UnS (98.0 ± 0.3%) compared to FeS (94.8 ± 1.6%), ZnS (93.1 ± 2.1%) and FeZnS (87.6 ± 6.0%) pups. Intestinal 59Fe retention was higher in ZnS (7.2 ± 0.8%) compared to UnS (3.8 ± 0.8%), FeS (4.6 ± 0.9%) and FeZnS (4.8 ± 0.3%) pups. FeS decreased DMT1 expression but ZnS and FeZnS had no effect. There was no effect on FPN. When compared to UnS, hephaestin expression was higher in both FeS and FeZnS pups. Pups born from Fe supplemented dams had better Fe status which resulted in decreased DMT1 expression and Fe absorption. Zn supplementation interfered with Fe absorption by trapping Fe in the intestine, potentially through altered FPN localization or reduced hephaestin activity. Maternal Zn supplementation alone or in combination with Fe may compromise neonatal Fe status.

1International Centre for Diarrheal Disease and Research, Bangladesh (ICDDR,B) 2Dept of Nutrition, University of California, Davis, CA, 95616

48

Arsenic-mediated Apoptosis of Murine T lymphocytes

Involving Activation of JNK

Anwarul Azim Akhand1, Khaled Hossain2 and Izumi Nakashima3

Arsenic is a heavy-metal substance that exists in nature in very small amounts. Although exists in small amounts, elevated level of the compound can naturally be found in ground water. In Bangladesh, arsenic thereby became a devastating pollutant that has caused an environmental tragedy in some areas where a large population has been drinking arsenic-contaminated ground water. The long-term health effects caused by arsenic exposure includes cancers of skin, bladder, kidney and lung, neurological effects, cardiovascular disease, immunotoxicity, and immunosuppression. All these disease-causing effects of arsenic may share a common molecular mechanism at an early stage. Here we report sodium arsenite (NaAsO2)-induced signal cascades from the cell surface to the nucleus of murine T lymphocytes. We observed that NaAsO2 induced apoptosis through fragmentation of DNA, activation of caspase, reciprocal regulation of Bcl-2/Bax with concomitant reduction of membrane potential. NaAsO2-induced caspase activation is dependent on the activity of c-Jun amino-terminal kinase (JNK). As an initial event of triggering the signal, NaAsO2 induced aggregation of GPI-anchored protein Thy-1 and production of superoxide. These results are very important for a better understanding of the molecular mechanism of NaAsO2-induced signal delivery pathway that would lead us to invention of a new therapeutic approach. 1Department of Genetic Engineering and Biotechnology, University of Dhaka, 2Department of Biochemistry, University of Rajshahi, Bangladesh and 3Department of Immunology, Nagoya University School of Medicine, Nagoya, Japan

49

Molecular Characterization and Clinical Evaluation of Dengue Outbreak

in Year 2002 in Bangladesh

M.A. Islam1, 5, M. U. Ahmed 2, N. Begum 3, N. A. Chowdhury 3, A. H. Khan 1, M. C. Parquet 1, S. Bipolo 1, S. Inoue 1, F. Hasebe 1, Y. Suzuki 4, 5 and K. Morita 1, 5

During the febrile illness epidemic in Bangladesh in 2002, 58 people died out of 6132 affected,

died. Two hundred hospitalized patients were analyzed clinically, serologically and

virologically to determine the features of this dengue infection. Among 10- to 70- year- old age

group of the 200 clinically suspected dengue patients, 100 (50%) were confirmed as dengue

cases by virus isolation and dengue IgM-capture ELISA. Of the 100 dengue confirmed cases, the

mean age was 29.0 (± 12.4). The possible dengue secondary infection rate determined by

Flavivirus IgG-indirect ELISA was 78% in 2002. Eight dengue virus strains were isolated,

representing the first dengue virus isolation in the country and all were dengue virus type-3

(DEN-3). Sequence data for the envelope gene of the DEN-3 Bangladeshi isolates were used in a

phylogenetic comparison with DEN-3 from other countries. A phylogenetic analysis revealed

that all 8 strains of DEN-3 were clustered within a well-supported independent sub-cluster of

genotype II and were closely related to Thai isolates from the 90’s. Therefore, it is likely that the

currently circulating DEN-3 viruses entered Bangladesh from neighboring countries.

Key Words: Molecular Characterization, Clinical Evaluation, Dengue

1 Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan,2

Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh,3

Shaheed Suhrawardi Hospital, Shere-e-Bangla Nagar, Dhaka, Bangladesh,4 Department of Biochemistry, School of

Pharmaceutical Science, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan, 5Core Research for

Evolutional Science and Technology (CREST), Japan

Name of corresponding author: Prof. Dr. Md. Alimul Islam

E.mail: [email protected] Fax: +88091-55810

50

Current Advances in Insulin Delivery and Formulation

Nadim Ashraf1 and Gary Adams2 The prevalence of diabetes in Bangladesh is estimated to be 5.2% among the adult population. It is estimated that almost 3 million people have diabetes in Bangladesh, among which about 90% - 95% belong to Type 2. For these population, an insulin regime is not only applied to Type1 diabetes patients but also to Type 2 patients, who remain persistently symptomatic hyperglycaemic on maximum dose of oral agents and diet. Since insulin was introduced into clinical practice in 1922, attempts at replicating physiological insulin secretion, as a means of restoring the normal metabolic milieu and thereby minimizing the risk of diabetic complications, has become an essential feature of insulin treatment. As our understanding of the importance of strict glycemic control has come into focus, our ability to achieve this goal has improved. Much of this newfound ability to obtain a level close to the euglycemic index in patients with diabetes can be attributed to novel insulin molecules that scientists have synthesized in the past decade and a half. Following the development of recombinant human insulin in the 1980s and insulin analogues in the 1990s, at present insulin therapy holds the promise of non-injection delivery methods that will become widely available in the near future. The evolution of insulin therapy already has produced more effective delivery methods for better glycemic control with fewer side effects. Current research in the insulin arena has focused on new formulations, novel insulin molecules, and various methods of insulin delivery that more closely approximate normal physiologic insulin response than conventional preparations. This paper will review on the recent development of insulin formulation and the current advances in the alternative approaches to insulin administration that are under consideration, with the goal to replicate physiological patterns of insulin secretion and free the patients from the increasingly complex and intensive insulin regimens involving multiple daily injections. Key words: Diabetes; Insulin; Delivery; Insulin formulation. 1School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough Leics, LE12 5RD, UK 2Insulin and Diabetes Experimental Research (IDER) Group, University of Nottingham, Faculty of Medicine and Health Science, Clifton Boulevard, Nottingham, NG7 2UH, UK Name of the corresponding author: Nadim Ashraf E-mail: [email protected]. Tel: + 44 (0) 115 951 6400, Fax: +44 (0) 115 951 6032

51


M.S.R. Khan1, A. Tanaka A3,H Ide3 , K Hoshinoo2, Y Hanafusa3 , Y Tagawa2

The major outer membrane protein (MOMP) of Haemophilus somnus shows antigenic and molecular mass diversity that forms the basis of a preliminary grouping system for H. somnus strains. In this study, the gene encoding MOMP of H. somnus strain 8025 was cloned in three overlapping fragments by PCR techniques, and then sequenced. The gene consists of a 1164-bp open reading frame encoding a deduced 380-amino acid protein with a 19-amino acid signal sequence, giving a mature protein with a calculated molecular mass of 39,913 Da. Significant homology was found between MOMP and porin protein sequences of bacteria in Pasteurellaceae species. When expressed in Escherichia coli, the protein from the MOMP gene directed by the T7 promoter was identical in size (approximately 40 kDa) to native MOMP and reacted with MOMP-specific antibodies. Comparisons of the MOMP gene sequences from six unrelated strains of H. somnus to that of strain 8025 revealed that the genes of three MOMP type 1 strains were highly conserved with that of strain 8025 in length and sequence. However, two MOMP type 3c strains and one MOMP type 3a strain differed markedly from the MOMP of strain 8025 in their 3'-terminal halves. Their deduced MOMP amino acid sequences differed in sequence (3c, 80.5 and 82.7% identity; 3a, 62.4% identity) and in length (3c, 384 and 376; 3a, 316), indicating that the molecular differences are the basis of antigenicity and molecular mass differences of H. somnus MOMP. In the predicted MOMP secondary structure, the variable sequences primarily mapped to putative surface-exposed loops, and a variable and surface-exposed epitope of MOMP-specific antibody was identified in the seventh-largest loop. These findings are useful for understanding the structural and immunological characteristics of H. somnus. Key words: Haemophilus somnus, MOMP gene, PCR cloning

1. Department of Microbiology and Hygiene, Bangladesh Agricultural University, Faculty of Veterinary Science,

Mymensingh-2202, Bangladesh 2. Bacterial Disease Section, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan 3. Department of Integrative Environmental Sciences, Graduate School of Life and Environmental Sciences, University

of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan Name of corresponding author: Professor Dr. M. S.R. Khan E.mail: [email protected] Fax:+88091-55810

52

Large-Scale Identification of Protein-Protein Interaction of Escherichia coli K-12

Mohammad Arifuzzaman1, Maki Maeda3, Rintarou Saito4, Shigehiko Kanaya5, Chieko Wada6, Hirotada Mori2, 3

E. coli is one of the most well characterized organisms and has been used as a model system to study various aspects of bacterial physiology and genetics of fundamental and applied interest. Among the 4,339 ORFs (open reading frames) predicted in E. coli, nearly 50% of these ORFs are experimentally uncharacterized. In addition to functional analysis of individual ORFs, systematic analyses of relationships between constituent elements, such as gene regulatory networks, protein-protein interactions (PPIs) and metabolic networks, has not been possible before the post-genomic era. Protein-protein interactions play key roles in the structural and functional organization of a cell. A thorough description of these interactions should provide the foundations to facilitate elucidation of cellular activities, targeted drug design and cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged E. coli ORF clone library and 2,667 out of 4,339 proteins were successfully analyzed their putative interaction with target proteins on Ni2+-NTA column. Co-purified putative interacting proteins were identified by MALDI-TOF MS. Total 11,511 putative protein complexes were identified from 2,667 baits and expression of 798 previously hypothetical proteins. We analyzed our protein pair data using biological information and revealed many novel protein-protein interaction candidates. An extended analysis of these putative interaction networks, by both bioinformatics and experimental approaches, will provide novel strategies for investigations in systems biology.

Keywords: ASKA library/E. coli/post genome analysis/protein interaction network/pull down assay

1 Department of Biochemistry and Biotechnology, University of Science and Technology, Chittagong (USTC), Foy’s Lake, Chittagong, Bangladesh 2 Department of Biosciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan 3CREST, JST (Japan Science and Technology), Kawaguchi, Saitama 332-0012, Japan 4Institute of Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0035, Japan 5Department of Bioinformatics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan 6Institute for Virus Research, Kyoto University, Sakyo, Kyoto 606-8507, Japan Name of the corresponding author: Hirotada Mori e-mail: [email protected]

53


Abdullah Al Mueen¹, Md. Shamsuzzoha Bayzid¹, Md. Maksudul Alam¹ and Md. Saidur Rahman¹

A Single Nucleotide Polymorphism (SNP) is a variation in a single nucleotide that can occur within a person's DNA sequence. Haplotype is a pattern of SNPs on a DNA sequence. Constructing a pair of haplotypes that represent the SNP patterns of the inherited DNAs of an individual is referred as individual haplotyping. Haplotyping is an important recent technique which is used in DNA fingerprinting for criminal or parental verification, study of evolution and pharmacogenetics. Haplotyping from aligned and overlapping but erroneous fragments of the chromosomal sequences is a non-trivial problem. Minimum Error Correction (MEC) is a computational method for haplotyping, which minimizes the number of errors to be corrected so that the pair of haplotypes can be constructed through consensus of the fragments. In this paper, we give a heuristic algorithm for haplotyping through minimum error correction. Our algorithm iteratively searches for a better solution by maximizing a gain measure and stops whenever no better solution can be achieved. Time complexity of each iteration is O(m³k), where m represents the number of fragments and k represents the number of SNPs. We also give another gain measure which reduces the time complexity to O(m²k) without affecting the performance much. We tested our algorithm over a set of real haplotypes and another set of simulated haplotypes. The accuracy of our result is comparable to that of the most recent genetic algorithm. We had up to 100% accurate construction of haplotypes in our experiments in some cases. Moreover, our algorithm, which is deterministic in nature, is significantly faster than the known genetic algorithm. It takes less than 1 second for haplotypes with 964 bases whereas the known genetic algorithm takes over 100 seconds when the programs are executed on a Pentium III processor. Key words: Algorithm; Bioinformatics; DNA sequence; SNP; Haplotype; Minimum Error Correction; Heuristic; Gain; Accuracy; 1Department of Computer Science and Engineering, Bangladesh University of Engineering and Technology, Dhaka 1000, Bangladesh. Name of corresponding author: Md. Saidur Rahman Email: [email protected]

54

Development of Herbal Feed Additives for Ruminants

K. S. Huqueδ and Nasrin Sultanaδ The present work was conducted with an objective to determine the effect of native herbals as ruminant feed additives for boosting beef production of native growing cattle. Two groups of growing bulls each with six animals were fed with a basal diet of adlib urea-molasses-straw (UMS) supplemented with a concentrate mixture. Keeping a group control, Mukorossi herbal additive (collected and prepared from native plant sources) was added with the concentrate of the other group, and considered as the treatment diet. Addition of Mukorossi did not affect the intake of nutrients by animals or digestibility in vivo, but it reduced the rumen fauna (Ciliated protozoa) significantly (p<0.05) and improved rumen fiber degradability in sacco at early incubation hours. Feeding control or Mukorossi diets to two groups animal having initial average live weight of 113.0 Kg or 114.0 Kg, respectively resulted in a non-significant (p>0.05) difference in final live weight (160.7 Kg vs 174.0 Kg, respectively) or average daily live weight gain (483.2g vs 612.0g/head, respectively). The average extent of difference in final live weight was about 13.3 Kg/head and, that in daily live weight gain was 128.8g/head resulting in feed conversion ratios of 9.70 vs 8.70, respectively. About 26.7% higher growth response of Mukorossi feeding may be cost effective than using growth hormones, the growth response of which may vary from 15 to 20%. Mukorossi feeding significantly (p<0.05) increased blood glucose level (73.7 mg/dl vs 83.7mg/dl) after 2 hours of morning feeding, and it may be justified by the increased degradability in sacco of straw in the rumen. No significant differences in the level of blood glucose before morning feed or zero urinary glucose loss irrespective of feeding times may have nullified the possibility of any effect of Mukorossi on glucose loss through urine. From the above results and discussion it may be stated that Mukorossi may be a good herbal feed additive for the growing beef cattle without any apparent deleterious effect on animal health. Further research works are essential to conduct to find effective herbal sources with their easy availability of biomass for processing and manufacturing of cost effective additives for feeding ruminants. Key words: Herbal additive, Protozoa, Blood Glucose, Digestibility, Growth

δ Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh; Name of the corresponding author: Dr. K. S. Huque, e-mail: [email protected]

55

Biodegradation of Tannery Effluents by Bacteria A.K.Saha1, M.A.Mannan 1 , and M.K. Mahanta 1

Biodegradation of tannery effluents containing cadmium, chromium and lead nitrate by bacteria was investigated. Sample of effluents with cadmium, chromium and lead-nitrate (10 µg/ml) were incubated in mineral salts medium at 370C for 4 days and bacterial strain was isolated from the sample. The optimum pH 7.0 and temperature 370C are suitable for the growth of the bacteria. Isolated bacteria are resistant to cephradine and amoxycillin and utilize different carbohydrates viz. xylose, glucose. fructose, lactose, sucrose, mannose, galactose and maltose. The effluents are so toxic that fishes cannot survive in it even for two hours time. Even after dilution of the effluents by fresh water the same type of fishes cannot survive for the same duration. But after release of the isolated bacteria in the diluted effluents the same type of fishes survives for longer period. It was also found that fishes survived longer period after adding small quantity of glucose in the effluents. Cured bacteria were unable to grow on media containing cadmium, chromium, and lead nitrate. It may be conclude that isolated bacteria are able to degrade the effluents and the ability might be borne on plasmid. Key words: Tannery; effluents; bacteria; plasmid

1 Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh.

Name of corresponding author: Dr. A. K. Saha

E.mail: [email protected] Fax:0721-750064

56

Genetic Variation of Wild and Hatchery Populations of Indian Major carp, catla (Catla catla Hamilton) Revealed by RAPD Markers

Dr. Md. Mukhlesur Rahman Khan1

Randomly amplified polymorphic DNA (RAPD) markers were used to assess genetic variations in three wild viz. the Halda, the Jamuna and the Padma rivers and one hatchery population of Indian major carp, Catla catla (Hamilton). Five decamer random primers were used to amplify RAPD markers from 30 samples of each population. Out of 55 scorable bands, 30 were detected as polymorphic indicating some degree of genetic variation in all the studied populations. The estimated values of proportion of polymorphic loci and gene diversity values estimated were 54.55% and 0.23 for the Halda; 45.45% and 0.17 for the Jamuna; 49.09% and 0.20 for the Padma; and 41.82% and 0.17 for hatchery populations, respectively reflecting relatively a higher level of genetic variation in the Halda population. Among the polymorphic loci, 16 were observed to cause significant departure from homogeneity in all the population pairs indicating the presence of some degree of divergence in the populations. The inter-population similarity indices, gene flow and genetic distance values showed that the Jamuna-Pamda population pair of catla was genetically closer than other wild population pairs. The pair wise population differentiation (FST) values indicated a low level of genetic differentiation between the population pairs. The UPGMA dendrogram constructed from Nei’s genetic distances showed segregation of populations into two distinct clusters: the Halda alone was in one cluster while the Jamuna, the Padma and hatchery populations formed the second cluster. Understanding of genetic variation of the three wild populations and a representative hatchery population of catla would be supportive for management of the populations to facilitate maintenance of their genetic quality.

1Dr. Md. Mukhlesur Rahman Khan Associate Professor & Head Department of Fisheries Biology & Genetics Bangladesh Agricultural University Mymensingh-2202 Mobile-01711-407359 e.mail: [email protected]

57

Study on RAPD Analysis of Selective Goat Population

M.A.I. Talukder;1 B.B. Roy2 and K.M. Hossain3 In this study, RAPD-PCR conditions were evaluated to verify the genetic diversity of Black Bengal goat population. Blood samples of 25 pure breed animals were collected form Savar Goat Farm, Directorate of Livestock Services. DNA was extracted from blood and quality and quantity was checked by spectrophotometric measurement. 10 random primers were screened using 3 set of PCR conditions. Only 3 primers amplified scorable polymorphic bands. The fragment length of the products ranged from 250 bp to 680 bp. Primer BLRI 4 amplified two bands (500bp and 680bp) in all samples. Allelic frequencies were calculated by assuming that the population was in H-W equilibrium. Allelic frequencies varied from the lowest of 0.0513 to highest 0.9487. Total polymorphic loci were 66.67% Genetic diversity of the goat population was as low as 0.3680+0.1835. The relatively low level of genetic diversity of Black Bengal indicates that Black Bengal goat has been bred without any crossbreeding with foreign breeds after its domestication. In addition, it is likely that Black Bengal goat has experienced the loss of variation owing to non-random mating and/or genetic drift. DNA fingerprints generated in this study were used for identification of this breed. The application of such fingerprints can be identify of mixing of sheep meat in goat meat, which is now a very common practice in slaughterhouses of the country. 1. Principal Scientific Officer, Goat & Sheep Production Research Division, BLRI, Savar, Dhaka-1341. 2. M.Sc. Student, Biotechnology & Genetic Engineering Discipline, Khunla University, Khulna. 3. Professor, Biotechnology & Genetic Engineering Discipline, Khunla University, Khulna.

58

Development of Somaclonal Variants of Strawberry Adaptive to the Agro- ecological Condition of Bangladesh.

M. Hossain1, U.K. Roy1, R. Karim1, MK Biswas1, N Nahar3, A Hoque2 and R Islam1

Strawberry, Fragaria x ananassa (Duch.) is a natural hybrid of Fragaria chiloensis (L.) P. Mill. and Fragaria virginiana (Duch.). Fragaria is a member of the Rosaceae family. Strawberry is one of the most important and popular fruit in the temperate countries of the world due to its fragrance, test and nutritional properties. Objective of this study was to develop a new strawberry cultivar from F. x ananassa cultivars cultivated in Honshu region of Japan through the induction and selection of somaclonal variation. In initial step eight Japanese strawberry cultivars were grown for adaptive evaluation and one cultivar was selected that showed better adaptability to the environmental condition of Rajshahi. Callus was induced from runner’s nodal segment of the selected cultivar onto agar gelled MS medium supplemented with 0.2 – 3.0 mg/l NAA. Shoot regeneration was achieved by repeated sub-culturing of the calli onto MS medium supplemented with BAP (0.05 – 1.5 mg/l). The shoots were rooted individually on half MSO medium. The regenerated plants were transplanted to field and were evaluated for the occurrence of somaclonal variation using flowering and fruiting ability, adaptability and sustainability in summer and rainy season of Bangladesh, as parameters. Wide range of somaclonal variations in terms of morphological and agronomical characters were observed among the plants. Initially nine vernelization independent flowering plant types with better agronomic features were selected. The selected lines were evaluated for overcoming harsh summer and hot-humid rainy season. Out of nine, three lines (ST1, ST2 and ST3) were selected for commercial cultivation for subsequent years. These somaclones are distinct from each other in terms of fruit and other agronomical characters, and have potential for commercial cultivation in Bangladesh. Key words: Strawberry, somaclonal variation, callus 1. Plant Breeding and Gene Engineering Lab., Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh. 2. Professor, Dept. of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi-6205, Bangladesh. 3. PSO, Aman Tissue Culture LTD., Narikelbaria, Rajshahi Name of corresponding author: M. Monzur Hossain E-mail: [email protected]

59

Development and Evaluation of Transgenic Lettuce Expressing Salmon calcitonin (sCT) and Human calcitonin related peptide (hCGRP) Genes.

A. Hoque2, M. Hossain1, MB. Ahmed1, MK. Biswas1 and R. Islam1

Calcitonin (CT) is an endogenous polypeotide hormone plays a crucial role in both calcium homeostasis and bone remodeling and CGRP is a neuro-peptide and potent vasodilator, also plays very important role in calcium metabolism and believed to reduce blood pressure. The 1st and 2nd true leaves from 14-day-old in vitro grown lettuce seedlings were infected with A. tumefacience LBA4404 containing sCT and CGRP gene cassettes in pCAMBIA2301 Ti plasmids. Infected leaves were induced to develop callus within 2 week of culture and subsequently started to develop shoot buds within 6 weeks of culture initiation. Regenerated shoots were individually rooted onto rooting medium contained 50 mg/l Kanamycin. The genomic DNA amplified with sCT specific primer reveals the presence of sCT gene in 37 out of 66 kanamycin resistant plantlets. Similarly, genomic DNA of 50 kanamycin resistant plantlets amplified with CGRP specific primers reveals the presence of CGRP gene among 39 plantlets. These plants were transferred to net house and grown to maturity. Transcripts of both sCT and CGRP in the To plants were analyzed by RT-PCR. RT-PCR profiles of To plants generated through sCT specific primers exhibits the presence of expected size cDNA of sCT gene in 22 out of 28 plants tested. Similar expression of the transcript of CGRP gene in 32 out of 35 plants tested was also demonstrated. Presence study clearly demonstrated that both sCT and CGRP genes could express their transcript. Further evaluation for the detection of peptides to corresponding to both genes is in under way. Key words: Salmon calcitonin (sCT), human calcitonin related peptide (hCGRP), lettuce 1. Plant Breeding and Gene Engineering Lab., Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh. 2. Professor, Dept. of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi-6205, Bangladesh. Name of corresponding author: Md. Aminul Hoque E-mail: [email protected]

60

Abstract

Promotional

61


Prof. Virander Singh Chauhana

Indian Biotechnology scenario has grown quite rapidly in the last decade or so and presently ranks third in the region after Japan and Korea. This has become possible with numerous initiatives taken by the Department of Biotechnology, Government of India, and the strong base of trained scientific manpower in the country. The Biotech sector in India comprises of around 280 companies generating revenue of 41.5 billion and is expected to grow around 30-35% each year. ICGEB plays a crucial role in promoting research and capacity building in biotechnology for the developing world. Mandated to carry out research, training and development of IP and technologies for the developing world in the field of biotechnology and genetic engineering, ICGEB has been successful in building a strong capacity of scientific manpower by training around 5000 researchers and students in the ICGEB member states. Indian biotech scene and research programmes at ICGEB will be discussed a International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi – 110067, India Email: [email protected]

62

Biotechnology Initiatives in Pakistan

Dr. Anwar Nasima Pakistan is a country of over 160 million people. It has predominantly agriculture-based economy that accounts for the 24% of GDP. It covers a total area of 19,671 million acres of which 5,411 million acres are cultivated. Using traditional means our ancestors have been breeding animals & growing crops of different varieties, however these common practices have damaged the cultivated land through water and wind erosion, salination and water logging. To overcome the impending shortage in this field conventional methodologies have to undergo a paradigm shift. Thus the government is primarily focusing on various aspects of agricultural biotechnology. Pakistan has a solid history of engagement with traditional biotechnology. Many new plant varieties, some of which are used all over the country. Coordinated efforts at the Government level have laid strong foundations for Biotechnology. The Ministry of Science and Technology has infact already invested more than Rs. 1 billion in various projects. There are 29 Government Institutes/Organization & Universities working in the field of Biotechnology. The main thrust of most of these institutes has been the development of genetically modified crops that are best suited to our environment and can increase the productivity of the crops with minimal environmental hazards. Realizing the immense potential of Biotechnology, in the third meeting of the National Commission for Science and Technology chaired by the President of Pakistan, Biotechnology was declared one of the highest priorities among the selected research fields. Since Pakistan has an agro-based economy, the Federal investment has been more focused in agricultural field. Pakistan has also established a National Commission on Biotechnology. The National Commission on Biotechnology is an advisory body to the Ministry of Science and Technology to monitor new developments in the field of Biotechnology at national and international level and to recommend appropriate measures for the benefit of the country. The Commission comprises of 15 members which includes expert Agriculturists, Environmentalists, Industrialists & Scientists. The main Objective of the Commission are:

i. Human Resource Development. ii. To strengthen Government-Private sector collaboration. iii. Publications, T.V. and Radio Programmes.

It has been decided that initially special attention should be paid to human resource development and in view of the limited resources Agriculture and Health will be the top priority areas. Further more a comprehensive National Policy and Action Plan has been developed. In Industrial Biotechnology the focus is on encouraging the industry for indigenous development and production of probes, primers/ ELISA plates, monoclonal antibodies, enzymes and other reagents required for diagnosis of local strains of causative agents for malaria hepatitis, cholera and tuberculosis. Considering the profound importance of research and development in the health arena, investment is now being focused on vaccine development and production of diagnostic kits. Development policies encouraging conducive environment for biotechnology industrial growth have been introduced. Continued government efforts are ensuring a sustainable development of Biotechnology in Pakistan.

aAdvisor, COMSTECH, Pakistan

63

Life Sciences and Biotechnology in Turkey

Mehmet Ozturk, Ph. D.a

Modern biotechnology takes its roots from the scientific discoveries and technical advances in the field of life sciences. This is why the biotechnology initiatives in the developed countries are based on science and technology policies where the science is defined as either molecular biology or lifer sciences, and the technology means biotechnology. It is now well accepted that this “science and technology” policy is highly successful in bringing the humanity to “the age of biotechnology”. Unfortunately, the biotechnology does not serve the humanity equally well, as less developed countries are becoming more and more dependent on imported biotechnological products in medicine as well as agriculture. Such countries are trying to solve this growing problem by national biotechnology programs for local production of key biotechnological products. Biotechnology has also been on the agenda of Turkey for the last 30 years. However, the progress was modest and did not make an impact on Turkish economy. Many believe that the poor success of biotechnology policies in developing countries results from ill-defined national strategies. The main weakness of such strategies is the expectation that it is possible to develop biotechnological industry by investing on technology only. The investment on science is disregarded in most developing counties because it is “expensive”, “slow to produce”, or even “useless” for the society. Turkey, after following such a strategy for many years is now shifting its priorities to developing its science and technology hand by hand. One of the earlier consequences of this strategy was the high rate of increase in the scientific production of Turkey. Our country is in the 19th position in the World with nearly 20.000 SCI papers published in 2005. The change of policy is reflected in biotechnology by the development of new undergraduate and graduate programs in molecular biology, biotechnology and nanobiotechnology. These programs are complemented by a recent and unprecedented increase in research funding, state-supported technoparks for technology companies nearby universities, taxes exemptions for technology-based investments, and the participation to the research framework programs of the European Union. I will discuss these new developments together with their potential impacts on the development of biotechnology in the country. a Bilkent University, Department of Molecular Biology and Genetics, 06800 Ankara, Turkey

64

Integrating excellence in molecular science and technology to generate Knowledge-based industries:

A Malaysian experience in diagnostics

Prof Asma Ismaila In the knowledge era, the challenge in R&D is to focus on the development of original knowledge-based products that can compete in the global market. Development of commercially viable, patented knowledge-based products within a university environment requires the innovation system and innovation policies to be in place and a change in the paradigm towards research approach. A crucial agent towards the success of the innovation system is the development and training of the human capital that would be the future drivers of the Knowledge-based industry. Awareness of intellectual property rights, the need for original research, indigenous platforms, entrepreneurship as well as developing and strengthening self confidence and leadership are among the factors needed towards the training of K-workers facing the new economy. For K-based products to fare well in developing countries, it is imperative that these products are developed and designed with the clients need in mind. Enhancement of the value chain can be achieved with the existence of innovation policies of the country and venture capitalists willing to invest in indigenous biotech products and innovations. The paper will also discuss the Malaysian experience in the R&D strategies and commercialization of indigenous diagnostics towards wealth creation and improvement in the quality of life. For developing countries to survive K-economy, it is important that researchers develop the capacity for technology development rather than just being users of technology.

aDirector, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia Health campus, 16150 Kubang Kerian , Kelantan, or Suite 110 Eureka Building, 11800 Minden, Penang Malaysia

65

Biotechnology Capacity Development in Scientifically Lagging Countries of Asia and Africa through the Establishment of a Drug

Discovery and Development Research Network Based On Traditional Knowledge and Indigenous Resources

Ahmed A. Azada

Bangladesh and most of the scientifically lagging and least developed countries of the developing world are largely concentrated in a geographical region that includes Southern and Central Asia, the Near East, North Africa and Sub-Saharan Africa, a region that can be loosely termed the “OIC region” as it is also home to the overwhelming majority of the OIC-member countries. The poorer and least developed countries in the OIC region, including Bangladesh, are in the greatest need of effective and affordable drugs and vaccines against diseases that continue to cause devastation to the health and economies of these countries. Unfortunately these countries are unable to carry out effective indigenous research to meet their needs for new drugs because of their weakness in scientific proficiency and lack of research capacity.

This is a proposal for the establishment of a Drug Discovery and Development Programme in the OIC region with a major focus on research capacity development. The initiative will draw on indigenous knowledge, natural products and existing scientific resources, and will foster multidisciplinary research collaboration between diverse research groups with complementary expertise and facilities. The drug development process, even before commercialization, is extremely expensive and requires extensive capacity development. This is beyond the capability of any single scientifically lagging country, but could be achieved through focused collaboration within a regional research network. This major initiative could result in much needed therapeutic products and, in collaboration with local industry partners, lead to the establishment of a research-based pharmaceutical industry dedicated to the health care needs of the region. Cutting edge technologies in the molecular biosciences and core facilities set up for this initiative will also be available to all other biotechnology based activities in the region and lead to increased research productivity, capacity development and a well trained scientific manpower. These are all very important steps needed to raise the science and technology base to the level required to transform the region from being scientifically lagging to being scientifically proficient. Since scientific and technological proficiency underpins sustainable development, this initiative should be treated as a priority by regional governments, including the Government of Bangladesh, and supported by national (BAS) and international science academies (TWAS, IAS, AAS), multinational science organizations (ICGEB, COMSTECH), regional development banks (IDB, ADB) and international development partners that subscribe to science and technology-based sustainable development in the least developed and scientifically lagging countries. aFTWAS, FIAS, FBAS, MASSAf, FRSSA TWAS Research Professor, BRAC University, Dhaka, Bangladesh [email protected]

66


A. K. Azada

The Government of Bangladesh recently approved the National Guidelines for Medical Biotechnology (MBT) subject to implementation within the framework of National Biotechnology Policy. The guidelines focus on public-private partnership to exploit the benefits of medical biotechnology in the country. The guidelines caution for protection of human health and nutrition, privacy and personal rights, and religious and cultural values through effective regulatory system and public awareness. The guidelines also include a three-phase action plan for implementation of MBT in Bangladesh. The first phase will be implemented by one year, which aims for assessment, sensitization and creation of background environment for future MBT development in the country. The second phase will be implemented by 2010, when basic to moderate levels of infrastructure and capacity for MBT will be developed. In the third phase to be implemented by 2025, advanced infrastructure and capacity will be developed to enable attainment of MBT in Bangladesh to the globally competitive level. This paper discusses, in detail, the national guidelines on medical biotechnology in Bangladesh. Key words: National; Guidelines; Medical Biotechnology; Regulatory System; Public Awareness; Action Plan; Bangladesh

aDepartment of Biochemistry, National Institute of Cardiovascular Diseases (NICVD), Shere Bangla Nagar, Dhaka, MOHFW, Bangladesh Email: [email protected]

67

Importance of Laboratory Tests to a Country’s Healthcare System and its Accreditation – What It is and How to Get It.

Jalaluddin Bhuiyan1

It is important for a medical and cost effective point of view for a country to have an infrastructure in place that provides qualified manpower and monitors the quality of the testing in its clinical laboratories. This can be used to bring about the standardization of critical analytes that are utilized in the diagnosis and management of those diseases that are of greatest burden to the country’s healthcare system. At an optimum a country should also have an accreditation program in place as part of a requirement for the licensing of its medical laboratories. There are many different accreditation bodies, like CAP, COLA, ISO 15189, QMPLS and others and that ISO 15189 will eventually be the de-facto accreditation standard globally. For international clinical trials most laboratories are trying to get CAP accreditation, many also have registered under CLIA. In an ideal world, Bangladesh should develop an accreditation standard for its clinical laboratories and have a government standards setting body (like BSTI) confirm it as a national standard for the country. In Canada the body that does this is the SCC, in Mexico it is EMA. Through BSTI, Bangladesh subscribes to the ISO process as ‘Member bodies’ and it will be an obvious expectation to bring country’s clinical laboratories equivalent to the standards of ISO 15189. Corresponding author: Jalaluddin Bhuiyan, PhD, DABCC, FACB Head of Clinical Biochemistry, Department of Pathology & Laboratory Medicine, King Faisal Specialist Hospital & Research Center MBC 10, P.O. Box 3354, Riyadh 11211 Kingdom of Saudi Arabia Tel 966 1 442 4293 (Office) Mobile 966 503207589 Fax 966 1 442 4280 E-mail: [email protected]

68

Biotechnology Policy, Challenges and Opportunities and Research at Agricultural Research Institutes: An Overview

Dr. Md. Abdur Razzaquea

Modern biotechnology has opened up wide opportunities in the development of new and novel types of crops, improvement in farming practices, controlling pest and diseases through enhanced genetic resistance and use of bio-control agent. It provides effective tools for enhancing crop production by food security and poverty alleviation. An excellent prospect of biotechnology also exists for health and nutrition. To create congenial environment for encouraging Research and Development in biotechnology, Bangladesh Government has formulated biotechnology policy. The main goal of the policy is to ensure sustainable development of agriculture, food and other crops; nutrition, health, environment and livelihood of people, enhance agricultural competitiveness in relation to global standards. The other important goals include strengthening of national capabilities in modern biotechnology, biosafety and bioethics in order to ensure judicious use of this modern tool for socio-economic development of the country. With a view to increasing food production and to overcoming constraints of agricultural productivity, the country embarked on the development and application of relevant biotechnology at Agricultural Research Institutes. Bangladesh Agricultural Research Institute and Bangladesh Rice Research Institute have acquired biotechnology products developed in other countries and institute to improve our crop varieties. This requires environmentally sound trial system. Bangladesh gives more emphasis on effective risk assessments, risk management associated with the use, handling and transfer of living modified organism. Under the collaborative programme between Bangladesh Agricultural Research Institute and Agricultural Biotechnology Support Project II, trials on Bt eggplant seeds of 9 varieties and true potato seeds of two varieties of potato are being conducted in contained condition at the Bangladesh Agricultural Research Institute. Bangladesh Rice Research Institute is conducting BR29 golden rice trial in collaboration with International Rice Research Institute. The paper highlights on the biotechnology policy, biosafety guidelines, research programmes, challenges and opportunities of biotechnology and field trial guidelines for Genetically Modified Organism. aMember-Director (Crops), Bangladesh Agricultural Research Council

69


Michael Behan The WHO Intergovernmental Working Group on Public Health, Innovation and Intellectual Property Rights (IGWG) is now considering how to prioritize research and development of medicines for diseases predominantly affecting developing countries, and how to increase access to such new treatments. It will make recommendations to the World Health Assembly in early 2008 that will impact what new treatments are developed, how technologies are shared, and what tools will be available to countries for ensuring access to medicines for decades to come. IGWG Issues to be discussed by Michael Behan include:

A. Prioritizing diseases disproportionately affecting developing countries, to foster more investment in research and development (R&D) of treatments for such diseases;

B. Ensuring that existing flexibilities under TRIPS are preserved and that new mechanisms to increase access are examined, such as patent pools and the UNITAID model;

C. Exploring alternative ways to stimulate and reward R&D for diseases that disproportionately affect developing countries, including prize fund models; and

D. Exploring new standard-setting frameworks, alternative to TRIPS, for developing countries to meet appropriate obligations to fund medical R&D, such as the proposed Global Medical R&D Treaty.

70


Sharif Akhteruzzamana Forensic science involves many scientific disciplines to identify the source of a piece of evidence taken from a crime scene, victim, suspect or other potentially involved individuals in relation to a crime. The introduction of DNA technology in forensic science in mid 90’s has indeed revolutionized the criminal justice system. Personal identification based on variations that exist in DNA molecule has become as an established crime solving tool for police and prosecutors now a days. With its capacity to implicate and eliminate DNA evidence can play a vital role from the initiation of a case to the post-conviction confirmation of truth. In humans, about 99.9% of all 3.3 billion nucleotides we inherit from our parents are identical among individuals and differences exist in only 0.1% DNA. Forensic DNA analysis looks into these differences present as tandemly repeated clusters called VNTs (Variable Number of Tandem Repeats) and STRs (Short Tandem Repeats) in human genome. With the advent of PCR (Polymerase Chain Reaction), capillary electrophoresis and other molecular biological techniques it is now possible to generate DNA profile from traces of biological evidence like blood, semen, saliva, hair teeth or bone in 24 hours of time. The potential application of this technology includes identification of alleged murderer, sexual assault offender, mutilated dead bodied, missing children, disaster victims as well as resolving paternity disputes. Bangladesh is not far behind in introducing this technology in its legal system. The government has established the National Forensic DNA Profiling Laboratory, the first laboratory of its kind in Bangladesh in January, 2006. This laboratory therefore, is a vital addition to the techniques traditionally available to the investigators and would catapult the criminal justice of this country into a new era. aNational Forensic DNA Profiling Laboratory, Dhaka Medical College

71

Biochemical and genetic characterization of young onset diabetes in Bangladeshi population

Dr Liaquat Ali1

Considerable controversies exist regarding the nature of young onset diabetes in some tropical countries including Bangladesh. Biochemical and genetic characterization are important tools for resolving this controversy. We have investigated insulin secreting capacity and insulin sensitivity (the two basic determinants of glucose tolerance) in Bangladeshi population. A substantially lower insulin secretory capacity, both in absolute and relative terms, has been observed in the young lean (BMI 19-22) diabetic patients by glucagon stimulation test. These patients do not show any considerable insulin resistance with short insulin tolerance test. A tendency of insulin insensitivity was observed in these patients with higher BMI [Kitt values inversely correlated to BMI in diabetic subjects (r= -0.559, p=0.004)]. This finding is in contrast with that found in middle-aged (above 40 years) patients with higher BMI (25-27) in the same population who develop insulin deficiency and insulin resistance both. Thus it seems that insulin resistance is an important factor in the pathophysiology of diabetes in middle aged patients with normal to higher body weight, but insulin deficiency is the primary cause of diabetes in the lean and young diabetic patients of Bangladesh. To find out the genetic contribution in the pathophysiology of type 2 diabetes, normal glucose tolerant first-degree relatives of diabetic subjects have been studied for their insulin sensitivity and B cell function with the control subjects (without family history of diabetes). The data suggest that genetically determined insulin resistance may also be a basic pathophysiological mechanism for Type 2 diabetes mellitus in Bangladeshi population. Studies on autoimmunity has revealed that about a quarter of young diabetes subjects are positive for GAD ab and 12% for IA2 -ic Ab GAD Ab positive subjects show significantly lower C-peptide values compared to the negative ones. Some of the candidate genes for diabetes and pancreatitis have been investigated in the YDM subjects. The trypsinogen variants (R122H, A16V, N29I), associated with pancreatopathy, are absent in these subjects. However, about 12% of YDM subjects are positive for SPINK1 gene N34S mutation (p<0.001). YDM subjects do not show significant association with INS VNTR polymorphism. However, variant ‘A’ allele is found to have significantly higher fasting C-peptide levels [0.23 (0.02-1.32)] compared to those with wild ‘T’ allele [0.19 (0.03-1.13) (p<0.05). Polymorphic ‘A’ allele is also found to be significantly associated with GAD Ab negativity of the YDM subjects (p<0.018). Transcription factor gene variants, associated with neonatal and early childhood T1D [EIF2AK3 gene Indel15 AT+/AT-], and maturity onset diabetes of the young (MODY) [TCF1 (A98V), NEUROD1 (A45T) and NEUROG3 (G167R, S199F)], is not associated with diabetes in YDM. Studies on pancreatic morphology (by ERCP) and on exocrine pancreatic function (by secretin induced bicarbonate measurement or by fecal elastase-1 test) reveal that morphological and functional damage do not have a straightforward correlation with endocrine pancreatic dysfunction (assessed by fasting as well as glucagon/arginine stimualated C-peptide and glucagon measurement) in the Tropical Calcific Pancreatitis (TCP) and Fibrocalculus Pancreatic Diabetes (FCPD) patients. Transmission of HLA genes suggest both similarity and dissimilarity of FCPD with type 1 diabetes. This may suggest that diabetes in FCPD is not straightforward secondary diabetes as suggested in the recent classification of ADA and WHO Expert Committees. 1Dept of Biochemistry and Cell Biology, BIRDEM, 122 Kazi Nazrul Islam Avenue, Dhaka-1000, Bangladesh e-mail: [email protected]; [email protected]

72


M. A. Rashid1, M. A. Mazid1, M. S. Rahman1, M. R. Haque1

Pharmaceutical sciences, as a multidisciplinary subject, contributes to all aspects of modern health care systems by providing safe, effective as well as new drugs through basic and applied research. Realizing the potential role of pharmacists in the total health care system, pharmacy education in Bangladesh was introduced through the establishment of Department of Pharmacy in the University of Dhaka in 1964. Later on, the Department of Pharmacy was up-graded to the Faculty of Pharmacy in 1995 comprising of three departments namely, Department of Pharmaceutical Chemistry, Department of Pharmaceutical Technology, and Department of Clinical Pharmacy & Pharmacology to fulfill the increasing demands of specialized personnel and multidimensional contributions of this noble profession in our national health care program. We are extensively engaged in the discovery of new herbals and synthetics drugs, and the development of their assay methods using instrumental, pharmacological, clinical, and molecular biology & biotechnological approaches. Considering the recent progress in the field of biotechnology and biotechnological products such as vaccines, monoclonal antibodies, gene therapy and anti-sense drugs in the treatment of cancer, infectious and other life-threatening diseases, we are deeply concerned in the development of new biopharmaceuticals along with the natural and synthetic drugs and their quality control aspects in collaboration with other national research institutions, pharmaceutical industries and international organizations. As a part of our ongoing research, we have isolated and characterized 134 compounds, including 33 new molecules from 48 medicinal plants of Bangladesh, belonging to 27 families. Terpenoids were the major constituents among the isolated compounds. The crude extractives and several purified molecules obtained from some of these plant species demonstrated significant in vitro anitmicrobial activity and cytotoxicity. Further screening of these extractives and isolated compounds are under way to investigate their therapeutic values and establish them as herbal or natural drugs. Key words: Pharmacy, Research, Herbals, Biotechnology, Molecular Biological Approaches.

1. Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh Name of corresponding author: Dr. Md. Abdur Rashid Email: [email protected] Fax: 88-02-8612069

73

Resource-poor microbiology laboratories in Bangladesh: Prospects of Biotechnology.

Samir K. Saha, Ph.D.1

The molecular techniques have revolutionized the diagnosis, treatment, and epidemiology of infectious diseases. These techniques, however, are mostly unavailable in areas of the world where the prevalence of infectious diseases is the highest. Latest biotechnological tools are essential, at real time, in clinical laboratories for diagnostic and epidemiological purposes, Availability of these technologies and their proper use will have paramount implications in reducing child mortality. Nonetheless, in a country like Bangladesh, availability and rational use of these facilities is a dream rather than reality. Biotechnology, erroneously we believe, is always considered as an expensive endeavor and beyond the reach of the routine laboratories of developing countries. However, in the recent years, the cost, turn-around-time and complexity of performing molecular techniques is reducing. Our work in last few years, at Dhaka Shishu Hospital, has provided the proof of principal that state-of-the-art technology can be adapted in a resource poor environment and applied to address important public health issues. This extremely resource-poor laboratory has been contributing to health policy for children in the field of infectious diseases in Bangladesh and provides an example for other laboratories of the developing world. The Microbiology Laboratory of DSH, in turn, can transfer this technology to other laboratories of the country and beyond. At the same time, we are also interested to work with the experts of different fields, and explore the possibility of sharing the common facilities to improve the capacity of our laboratory in a rational way. This society, specifically the workshop, could be a milestone to form a multidisciplinary group to facilitate the promotion and application of biotechnology in an appropriate way. 1Department of Microbiology, Bangladesh Institute of Child Health, Dhaka Shishu (Children’s) Hospital, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.

74

Abstract

Posters

75

Detailed Contents Of Poster Presentations

(The documents are arranged according to their presentation sequence in the program)

Category Poster Title Authors Page No. P-1 Bioactivity guided search for

antimicrobial principles against multi-drug resistant pathogens from Swietenia mahagoni

A.K.M. Shahidur Rahman , M. Sawkat Ali, Hosne Ara Ali and A. K. Azad Chowdhury (Pharmacy, DU),

81

P-2 Molecular Characterization and Genetic Diversity of Rice using SSR and RAPD Markers

Abdul Quyyum Khan, M.M. Islam, M.S. Haque, S.N. Begum, M.A. Azam And R.M. Emon (BAU & BINA)

82

P-3 In Vitro Regeneration of Ginger Abu Naser Md. Mamun, Md. Iqbal Haque Swapan (BARI)

Abstract not

received P-4 Preservation And Maintenance of

Wood Quality Through Biotechnological Approaches

Ahmad Humayan Kabir (Botany, RU)

83

P-5 Some More Interesting ESTs from the cDNA Library of Corchorus olitorius L.

Ahmad S. Islam (Retired Professor, Botany, DU), Mohamad W. Wazni, J. Matthew Taliaferro, and K. Sathasivan

84

P-6 Meristem Culture of Potato Ahsan Hafiz (Head Of Biotechnology, Alpha Agro Limited)

Abstract not

received P-7 Circadian Changes in the Nucleoid

Structure and Clock Protein Localization in Synechococcus elongatus PCC 7942

Ali Azam Talukder and Takao Kondo (Microbiology, JU)

85

P-8 Role of Trichoderma in Composition of Garbage and in Agriculture.

G N M Ilias (N D S, Kapuriaputty)

Abstract not

received P-9 Screening of Aspergillus Flavus for

the Production of Aflatoxins B1and its Quantitation by TLC and ELISA Methods.

Gita Shrestha (Tribhuvan University, Kath, Nepal)

Abstract

not received

P-10 Efficacy of Gamma Radiations against Housefly (Musca Domestica L.) Reproduction and Survival I. Pupal Treatment

Halima Sadia Khan (Genetical Society Of Bangladesh)

86

P-11 Study of Artificial Breeding, Growth and Biochemical Genetic Analysis of Endangered Local Sarpunti, Puntius Sarana (Hamilton) for its Revival

Imran Parvez, Md. Mukhlesur Rahman Khan (HMDSTU & BAU)

87

P-12 Genetic and Biochemical Testing for Hereditary Breast and Ovarian Cancer

Jalaluddin Bhuiyan (Head, Clinical Biochem., King Faisal Hospital, SA)

88

P-13 A Comparative Study of the Genes Related to Long-Term Potentiation (Ltp) and Memory Development

Jesmin & Shahani Noor (GEB-DU)

89

76

P-14 Expression, Identification and Purification of Cry Protein from entomopathogenic Bacterium Bacilus thuringiensis israelensis.

Kazi Asraful Alam (MS Student, KU)

90

P-15 Analysis of 16s rRNA and Dehalognease Genes of Microbial Consortia Involved in Dechlorination of Chloroethenes

K H M Nazmul Hussain Nazir, F. Okamoto, H. Hashimoto, T. Futagami, M. Goto And K. Furukawa (BAU)

91

P-16 Salinity and drought resistant genes in potato: cloning, expression, transformation and variety development

KM Nasiruddin, R Begum, S K Sopory and A K Mattoo

(Biotechnology, BAU)

92

P-17 Gas Exchanges and Yield Responses of Mungbean (Vigna Radiata) Genotypes Differing In Flooding Tolerance

K.M. Shamsul Haque, M. Rafiqul Islam (BSMRAU)

Abstract

not received

P-18 Coastal Rice Landraces of Bangladesh: DNA Fingerprinting And Potential as Donor for Salt Tolerance Traits

Laisa Ahmed Lisa, Sazzadur Rahman, And Zeba Islam Seraj (BMB-DU, BRRI.)

93

P-19 Genetic structure of wild and hatchery populations of Indian major carp, mrigal (Cirrhinus cirrhosus) in Bangladesh.

M A Hasanat

94

P-20 Molecular Characterization of Soybean [Glycine Max (L.) Merr. ] Cultivars Using Microsatellite Markers for Plant Variety Protection

M.A.N.Nazim-Ud-Dowla, Md. Rezwan Molla, Mohammad Nazrul Islam, Md. Shefatur Rahman, Md. Samsul Alam And Lutfur Rahman (Fisheries Biology And Genetics, BAU)

95

P-21 Elevated Serum Levels of Heat Shock Proteins in Small Cell Lung Cancer

Md Abdul Khaleque (Radiation Oncology, BIDMC, Harvard Medical School, Boston, USA)

96

P-22 Evolutionary Trees and Their Generation

Md. Abdullah Adnan and Md. Saidur Rahman(CSE, BUET)

Abstract not

received P-23 Development of Novel Multiplex

Reverse Transcriptase-PCR Assays For Rapid Detection of Arboviruses

M. Alimul Islam S. Inoue, A. H. Khan, T. Nabeshima, A. Jittmittraphap, Aryati, N. Talemaitoga, Y. Jin, And K. Morita (BAU)

97

P-24 Antibiotic Susceptibility and Molecular Characterization of Salmonella Paratyphi A Isolated in Bangladesh

Md. Asaduzzaman, Kaisar Ali Talukder, Prof. A. K. Azad Chowdhury (Pharmacy, DU)

98

P-25 Antioxidant Activities of The Fruits of Manilkara Zapota (Sofeda), Aegle Marmelos (Bel), Phyllanthus Emblica (Amloki), Borassus Flabellifer (Tal) and Terminalia Chebula (Horitoki).

Md. Ashabul Islam (M.S Student, Biotechnology And Genetic Engineering, KU)

Abstract not

received

P-26 Need for production of Pharmaceutical and Industrial

Md. Ashraful Islam Bhuiya (GEB-DU)

99

77

Protein-A Bangladesh Prospectus. P-27 Genetic Diversity of Bitter Gourd

Germplasm of Bangladesh Md. Azizur Rahman (Horticulture, BAU)

100

P-28 Morphological, Physiological and Molecular Characterization of Phomopsis Vexans Isolates of Eggplant (Solanum Melongena) of Bangladesh

M. Bahadur Meah (BAU)

101

P-29 Isolation of Plasmid DNA from E. coli (JM109 & BL21DE3) for the Preparation of Recombinant DNA Cell

Md. Belal Hossain, J.W. Choi and K.S. Ryu ( Soil Science, BINA )

102

P-30 Production and Evaluation of Transgenic Lettuce with Pta (Pinellia Ternate Agglutinin), an Aphidicidal Gene.

Md. Bulbul Ahmed (M.S, Botany, RU)

Abstract

not received

P-31 Immune Cascade of Spodoptera Litura: Cloning, Expression And Characterization of Prophenol Oxidase Activating Enzyme 1(Ppael)

Md. Ekramul Hoque (Biotechnology, Sher-E-Bangla Agriculture University, Dhaka)

103

P-32 Prospect of Biotechnological Application in Bangladesh Tanning Industry

M. Fakhrul Alam (Editor, Bangladesh Leather)

Abstract not

received (a) Study on Interrelationship Among 10 Citrus Fruits Using RAPD

Md. Golam Rabbani (BAU), M. A. Maya, E. J. Garvey,

104

P-33

(b) Molecular Characterization of Sweet Gourd Using RAPD

E.H.M.S. Rahaman, M.G. Rabbani and E.J. Garvey

105

P-34 REP-PCR fingerprint of Lentil (Lens culinaris) Rhizobia isolated from Bangladesh

Md. Harun-or Rashid, M. A. Sattar and J.P.W. Young ( Soil Microbiology, BINA)

106

P-35 Red Cell Survival Study with Cr-51 Labeled Donor R.B.C Before and After Splenectomy of Thalassaemia Patients

Md. Israque Hossain Ansari (Bangladesh Atomic Energy Commission)

107

P-36 (a) Antibiogram, Plasmid Profiling Of E.Coli Isolate and Detection of Their Toxin Encoding Genes by Multiplex PCR. (b) In Vitro Propagation Of Pointed Goued(Trichosasthes Dioica Roxb)From Nobal Segments

Md. Jahangir Alam (BAU)

Abstract not

received P-37 Effect of Levels of Soya Milk on the

Storage Life of Dahi Prepared from Buffalo Milk

Md. Jamal Uddin, M. N. Sultana, S. Basak And M. R. Hassan (Biotechnology Division, BLRI)

108

P-38 Possible pathway (Hypothesis) of food chains in natural ecosystem through which human may be affected by arsenic poisoning

M. Mahfuzur Rahman and M. Azizur Rahman (Botany, JU)

109

P-39 Mechanistic Studies of Acetohydroxyacid Synthase (2.2.1.6)

78

from Mycobacterium Tuberculosis and Nicotinia Tabacum. New Potent Anti Tb Drug Findings by Ahas Gene Manipulation

Md. Masud Karim (Asst. Prof., University Of Development Alternative)

Abstract

not received

P-40 Genetic Divergences and Phylogenetic Relationships of Fejervarya Limnocharis Complex from Bangladesh and some Other Asian Countries Inferred from Mtdna Sequence and Allozyme Analyses.

Mafizul Islam (Hiroshima University)

110

(a) Agrobacteriam Mediated Transformation in Sugarcane of Bangladesh.

Md. Munan Shaik (BSRI)

111

P-41

(b) Effects of Soluble Dietary Mixture on Fiber Enriched Cookie Processing

Md. Munan Shaik (BSRI)

112

P-42 Some Organo-Chlorine Pollutants from Small Indigenous Floodplain Fishes of Bangladesh

M Niamul Naser (DU)

Abstract

not received

P-43 Impact of Hospital Liquid Waste Discharge in the Development of Antibiotic Resistance in the Environmental Bacteria.

M. N. Anwar, K. T. Osman and M. A. Hossain ( Microbiology, CU)

113

P-44 Role of PKA Sub Cellular Anchoring in the Glucagons-Induced Camp Stimulation of Insulin Secreting Pancreatic Cells

Md. Omar Faruque (BIRDEM), Dung Lenguyen, Anne-Dominique Lajoix, Eric Vives, Pierre Petit, Dominique Bataille, Liaquat Ali, El Habib Hani.

114

P-45 Present status of Biotechnology in Animal Production in Bangladesh

Md. Omar Faruque, S.A. Aziz, M.A. Alim (Animal Breeding and Genetics, BAU)

115

P-46 Commercial Enzyme Production by Recombinant DNA Technology; a Conceptual Works

M.R. Anower (BGE, KU)

116

P-47 In Vitro Propagation of Pointed Gourd (Trichosanthes Dioca Roxb.) from Nodal Segments

Prof. Md. Raihan Ali (BGE, KU)

Abstract not

received P-48 Collection, In Vitro Maturation,

Fertilization and Subsequent Development of Goat Oocytes in View of in Vitro Production (Ivp) of Embryos

Md. Rashedul Islam, S. Afroz, and M.A.M.Y. Khandoker (NIB, BAU)

117

P-49 Characterization of Different Strains of Common Carp (Cyprinus Carpio L.) (Cyprinidae, Cypriniformes) in Bangladesh Using Microsatellite DNA Markers

Md. Rashedul Kabir Mondol, , Md. Shahidul Islam And Md. Samsul Alam (Fisheries, RU)

118

P-50 Molecular Characterization of Groundnut (Arachis Hypogaea L.) Varieties Using SSR Markers for Variety Protection

Md. Rezwan Molla, Mohammad Nazrul Islam, Md. Shefatur Rahman, Md. Samsul Alam And Lutfur Rahman(Genetics And Plant Breeding, BAU)

119

P-51 Hori Dhan: Genetic Variation of Md. Sazzadur Rahman (BRRI) 120

79

Br11? P-52 Deduction of Coding Sequence of a

Jute Gene Using 5’ Race And Its Bioinformatics Analysis

Md. Shakhinur Islam, Nazlee Sharmin, Md Maksudul Alam (BMB & GEB-DU)

121

P-53 DNA Fingerprinting of Rice (Oryza Sativa L.) Cultivars Using Microsatellite Markers

Md. Shefatur Rahman, Md. Rezwan Molla And Lutfur Rahman (Genetics And Plant Breeding, BAU)

122

P-54 Endophytic Fungi are the Sources of Novel Pharmaceuticals

Md. Shoeb, M. Moshiuzzaman And N. Nahar (Chemistry, DU)

123

P-55 Genes Transcribed by Rhodococcus Equi Inside Macrophages and Under Selected Environmental Conditions

Md. Tanvir Rahman, V.M. Nicholson, And J.F. Prescott (Microbiology And Hygiene, BAU)

124

P-56 Association of Ins Vntr Polymorphism in Young Onset Diabetes Subjects Of Bangladesh

Md. Zahid Hassan M. I. Hawa, M. O. Faruque, K. B. Biswas, R. Islam, K. Azad, A. K. Azad Khan, L. Ali, R. D. G. Leslie, G. A. Hitman (BIRDEM)

125

P-57 Biological screening of spice materials for bioactivity against Tribolium castaneum (Hbst.) adults

N Islam, K Farhana, H Islam and E H Emran (Zoology, RU)

126

P-58 An Easy and Efficient DNA Isolation Protocol for Fingerprinting Using RAPD Markers of Sugarcane

Nadira Islam (BSRI, Pabna)

127

P-59 Overcoming Breeding Barriers of Interspecific Hybridization in Buckwheat: an Ultrastructural Analysis and Future Prospects

Nilufar Yasmin Shaikh And Taiji Adachi

128

P-60 Emergence of Optochin-resistant Streptococcus pneumoniae: Implications for Diagnosis and Management of Pneumococcal Diseases

Nishat Nasrin , Mahbubur Rahman, A. K. Azad Chowdhury (Pharmacy, DU)

129

P-61 Characterization of Insulin Secretory Defect in Bangladeshi Type 2 Diabetic Subjects

Rahelee Zinat, R. Karim, S. Islam, L. Ali (BIRDEM, CU.)

130

P-62 Fine Mapping and Progeny Testing in Rice to Identify the Flanking Markers for Introgression of Salt Tolerance

Rejbana Alam, Suhaila Rahman, Habibul Bari Sozib, Sazzadur Rahman Aliya Ferdousi, Zeba I. Seraj (BMB-DU)

131

P-63 Construction of Vectors with Different Genes and Promoters to Produce Salt Tolerant Rice By Transformation

Richard Malo, Mahzabin Amin, Rakibul Islam, Zeba I. Seraj (BMB-DU)

132

P-64 Glutinous And Non Glutinous Rice Of Bangladesh: DNA Fingerprinting And SNPs in The Waxy Gene

Rokeya Begum, Gazi Nurun Nahar, Zeba I. Seraj, Abed Chaudhury (BMB-DU.)

133

P-65 Rice Transformation With Antiporter Genes And Their Expression With Strong Promoter

Rumana Sultana Tammi, Lisa Parvin, Noorain Munim Rasul, Sharmin Jahan, Zeba I. Seraj (BMB-DU)

134

P-66 Functional analysis of GAMYB gene involved in gibberellin signaling in

S. M. Shahinul Islam et al (Institute of Biological Sciences,

135

80

rice. RU) P-67 Evaluation of Salt Tolerance in Rice

Using Phenotypic and Marker Assisted Selection

Salil Kumar Bhowmik (Biotechnology, BAU), M. M. Islam, R. M. Emon, S. N. Begum, A. Siddika And S. Sultana

136

P-68 Effects of Black and Green Tea Extracts (Polyphenols) on Arsenic-Induced Toxicity in Rabbits

Sheikh Zahir Raihan, A. K. Azad Chowdhury , Golam Hassan Rabbani, Farzana Marni, Md. Shawkat Ali (Pharmacy, DU)

137

P-69 Exocrine And Endocrine Pancreatic Function In T2d Patients Of Bangladesh

Soheli Sattar et al (Physiology and Molecular Biology, BIRDEM)

138

P-70 Expression and Purification of a Ruler Protein, Gp29, of Bacteriophage T4 By Two-Step Affinity Chromatography and its Identification by Maldi-Tof MS.

Subodh Kumar Sarkar, Shuji Kanamaru, Fumio Arisaka (USTC)

139

P-71 Zinc supplementation of pregnant rats with adequate zinc nutriture suppresses immune functions in offspring.

Taher Uddin (GEB-DU)

140

P-72 Chromatography Paper Strip Method for Collection, Transportation and Storage of Viral Genetic Material in Stool Samples

Tasnim Azim (Virology, ICDDR,B)

141

81

Bioactivity Guided Search for Antimicrobial Principles against Multi-Drug Resistant Pathogens from Swietenia mahagoni

A.K.M. Shahidur Rahmana, M. Sawkat Alia, Hosne Ara Alia and A. K. Azad Chowdhurya

Indiscriminate use of antibiotics in the developing world like Bangladesh has been the cause of the emergence of multidrug resistance pathogens such as Shigella, Salmonella,E.coli Staphylococcus, Streptococcus pneumoniae, Streptococcus â-hemolyticus etc. In search of molecules with antimicrobial activity (also cytotoxic principles) Swietenia mahagoni, belonging to a family (Maliaceae) known to possess antibacterial activity and antiparasitic activities has been subjected to bioactivity guided investigations. The methanolic extract of seeds of S. mahagoni was chromatographed on silica gel column, eluted with CH2Cl2 and CH3OH mixture in order of increasing polarity. The SMS1 (Rf = 0.25, silica gel-G: CHCl3), SMS2 (Rf = 0.50, silica gel-G: CHCl3). The structures of SMS1 and SMS2 were elucidated by spectra analyses (1H, 13C and DEPT135 NMR, Mass spectra) and CHN analyses. Table 1. Antibacterial activity of pure compounds SMS1, SMS2, SMLH1, SMLH3, SMLH8 isolated from Swietenia mahagoni against multi-drug resistant pathogens.

SMS1 SMS2 SMLH1 SMLH2 SMLH3 SMLH8 Test Bacteria 100 µgm/ml

100 µgm/ml

100 µgm/ml

100 µgm/ml

100 µgm/ml

100 µgm/ml

Salmonella Paratyphi a 19 15 -- -- -- -- Shigella dysenteriae b 16 14 -- -- 13 E.coli c 15 16 17 -- 12 -- Staphylococcus aureus d -- 16 17 -- -- 11 Bacillus subtilis e 19-8 19 -- -- -- -- Streptococcus â-hemolyticus f 16 12 10 13 11 13

a resistant to Ciprofloxacin, b resistant to Ciprofloxacin, c resistant to Cotrimoxazole, d resistant to Amoxycillin, e resistant to Amoxycillin, f resistant ot Ciprofloxacin, Cotrimoxazole, Amoxycillin, Erythromycin and Doxycyline The SMS1 and SMS2 spectra analysis by 1H, 13C and DEPT135 NMR analysis

SMS1: Proposed structure SMS2: Proposed structure

aDepartment of Clinical Pharmacy & Pharmacology, University of Dhaka, Dhaka, Bangladesh Address of correspondence : A.K. M. shahidur Rahman, M. Phil researcher, Department of Clinical Pharmay & Pharmacology, University of Dhaka, Dhaka, Bangladesh, e-mail: drshaheen20032003 @ yahoo.com , Phone: 1912175494

O

O

O

H

HO O

OHH

OH

OH8

30

1

3

5

9

O

O

O

HO O

OHH

O

OH

OO

8

30

1

3

5

9



82

Molecular Characterization and Genetic Diversity of Rice using SSR and RAPD Markers

A.Q. Khan1, M.M. Islam2, M.S. Haque1, S.N. Begum2, M.A. Azam2 and R.M. Emon2

Twenty diversed rice germplasms were used for this study to estimate genetic diversity using five SSR and five RAPD markers. Average polymorphic information content (PIC) value across the SSR alleles was 0.211 and average polymorphism for all microsatellite markers was 100%. The level of polymorphism as revealed by RAPD was 93.80%. The dendrogram constructed using unweighted pair group method of arithmetic means (UPGMA) on Nei’s genetic distance based on the SSR markers, clustered germplasms were grouped into three clusters; Binadhan-5, Binadhan-6, Iratom-24, BRRI Dhan 37 and BRRI Dhan 36 were grouped in cluster 1. The germplasms TNDB-100, Binashail, Chini Shagor, BRRI Dhan 41, RD-2586, Jongli Boro, BRRI Dhan-40 and Kalo Bhog formed cluster 2, and Dhol Kochuri, BINA Dhan-4, BRRI Dhan 38, Y-1281, Baowi Jhak, BRRI Dhan 32 and Kala Jira formed cluster 3. The dendrogram constructed using UPGMA on Nei’s genetic distance based on the RAPD markers, clustered germplasms were grouped into three clusters: BRRI Dhan 37, Iratom-24, BRRI Dhan 36, Binashail, RD-2586, BRRI Dhan 32, TNDB-100, BRRI Dhan 40 and Kala Jira were grouped in cluster 1; BRRI Dhan 38, BRRI Dhan 41, Chini Shagor, Baowi Jhak and Kalo Bhog were grouped in cluster 2; and Jongli Boro 581, BINA Dhan-4, BINA Dhan-5, BINA Dhan-6, Y-1281 and Dhol Kochuri were grouped in cluster 3. Both SSR and RAPD marker revealed lowest genetic distance (0.00 and 0.041) between BINA Dhan-5 and BINA Dhan-6 which suggests the lowest genetic diversity between these two germplasms. From the SSR and RAPD cluster analysis, SSR analysis showed more definite separation of clusters of genotype indicating a higher level of efficiency of these markers in determining relationships between germplasms that were not accurately differentiated by RAPD markers. Key words: Molecular Characterization; Genetic Diversity; SSR; RAPD; Rice

1Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh 2Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, P.O. Box 4, Mymensingh 2200, Bangladesh Name of corresponding author: A.Q. Khan

Email: [email protected]

83

Preservation and Maintenance of Wood Quality through Biotechnological Approaches

A. H. Kabir1 and M. Firoz Alam1

Wood is a versatile renewable resource, which has been extensively used as a reliable construction material and furniture ever since the beginning of civilization. The major disadvantage of wood is its susceptibility to biodeterioration by decay fungi, insects and bacteria. Anthocephalus cadamba (Kadam), Bombax ceiba (Shimul), Trewia nudiflora (Pithalu), Mangifera indica (Aam), Ziziphus jujuba (Boroi) are five locally available timber species of Bangladesh. Deterioration of wood can be prevented by artificially making the sapwood and heart wood toxic to wood enemies. The study of chemical preservative treatment of these timber species has been undertaken using mixture of Chromate-copper-boron (CCB) at 2:2:1 ratio with different preservative concentrations of 4.0%, 6.0%, 8.0%, and 10.0% with dipping duration of 8, 16 and 24 hours. It has been observed that Trewia nudiflora showed comparatively higher retention than other four species, which indicates that Trewia nudiflora is a permeable and diffusible timber species for dipping method. Preservative penetration test revealed that air dry wood sample using 10% preservative concentration with 8 hour and 24 hours duration of dipping has given best result for copper, while 10% preservative concentration with 24 hours duration used on green wood has shown best result for boron penetration. Fungitoxic activity of the leaf extract of Azadirachta indica (Neem) was also studied. Three different solvents, i.e. acetone, methyl alcohol, ethyl alcohol, were used to isolate the extract of neem leaves. The extractives were then used to see the inhibition activity over the test fungi in laboratory and natural condition. Both results proved that 3.0% concentration of acetone extract is best for inhibiting the growth of test fungus. Acetone and methanolic extract was also effective against that decay fungi. From this investigation, it is established that neem extract is potential source of biological preservative of wood that is environmentally sustainable. Key words: Wood preservation; Neem extract; Wood quality; CCB 1Department of Botany, Rajshahi University, Rajshahi 6205, Bangladesh Name of corresponding author: A. H. Kabir Email: [email protected] Phone: +8801717134836

84

Some more Interesting ESTs from the cDNA Library of Corchorus olitorius L.

Mohamad W. Waznia, J. Matthew Taliaferroa, Ahmad S. Islama and K. Sathasivana

Recently, we reported the complete open reading frame of 60S acidic ribosomal protein P3 and a partial cDNA sequence of class I chitinase in one of the two jute species, Corchorus olitorius var. O-4. 1 Analysis of another 150 samples were carried out using the same procedure except for increasing the time of digestion with PvuII from one to three hours. Allowing more time for digestion has increased the number of inserts many fold. Like last time, analysis of recombinant pBluescript plasmids was done using WII blast for similar base sequences in Arabidopsis thaliana and higher plants. One of the significant ESTs was 380 bp long with a score of 1674 and an E value of 1e-68. It showed 94% identity with similar ESTs in C. capsularis, recently deposited in the GenBank by Sen’s Laboratory in West Bengal, India (EST00136 Jute Pla...[gi:112136129]). Another EST associated with salt stress of Oryza sativa var. indica was 720 bp long with a score of 3490 and an E value of 2e–151. Other ESTs with values more than 800 and an E value of 3e –31 were ESTs from two cotton species, namely, Gossypium hirsutum and G. arboreum species. The results are interesting because the two genera Corchorus and Gossypium yield fiber of commerce. They belong to the family Tiliaceae and Malvaceae and are placed in the same order, Malvales indicating close phylogenetic relationship between Corchorus and Gossypium. aDepartment of Molecular, Cell and Developmental Biology, The University of Texas at Austin, USA-78712 _____________ J. Matthew Taliaferro, Ahmad S. Islam and K. Sathasivan (2006) Plant Tissue Cult. & Biotech. 16(2): 95-104.

85

Circadian Changes in the Nucleoid Structure and Clock Protein Localization in Synechococcus elongatus PCC 7942

Ali Azam Talukder §* and Takao Kondo §

In the cyanobacterium Synechococcus elongatus, almost all gene promoter activities are under the control of the circadian clock that is controlled by clock proteins KaiA, KaiB and KaiC. To elucidate if this genome-wide gene expression rhythms is associated with cyclic changes in the chromosome structure, we isolated Synechococcus nucleoid fractions biochemically and analyzed for the sedimentation rates, protein/DNA composition, structure as well as variation in localization of clock proteins under various growth conditions. Structure of the purified nucleoid changed in a circadian fashion with expanding and compacting around the time of transition from the subjective dusk and the subjective dawn, respectively. This rhythm coincided to the sedimentation pattern of hyracoid-associated nucleoids. Immunoblot analyses revealed that both the KaiC and the KaiB were associated with the nucleoid fraction, whereas KaiA was localized in both the nucleoid and the cytosolic fractions. Our results and previously available information imply the circadian changes of the structure of Synechococcus nucleoid. §Division of Biological Science, Graduate School of Science, Nagoya University, Japan. *Faculty of Biological Sciences, Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh. E-mail: [email protected]

86

Efficacy of Gamma Radiations Against Housefly (Musca Domestica L.) Reproduction And Survival I. Pupal Treatment

M. S. Islam1 and Halima Sadia Khan2

Musca domestica L. (Diptera: Muscidae), being a human commensal pest throughout the world, is of much concern to public health (Service, 1980). They are agents of a number of fatal diseases including typhoid, cholera, bacillary dysentery, amoebic dysentery, tuberculosis, anthrax opthalmia, infantile diarrhoea, leprosy, trachoma, gonorrhea, mastitis, pinkeye, as well as streptococcal and staphylococcal food borne illness. Although previous workers have studied the possible use of radiation of houseflies to effect population suppression (Magaudda et al. 1969; McDonald, 1970; McDonald & Overland, 1972; Flint & McDonald, 1972; Wagoner et al. 1974), more information and knowledge is needed to implement any practical irradiation control strategy of this pest species. Using gamma radiation doses of 0-10 Gy to pupal stadium, reproduction and survival parameters in the parents and subsequent progenies up to F3 generations of the common houseflies Musca domestica L. have been investigated. Compared to the untreated controls, irradiations significantly reduced egg laying, increased sterility and immature mortality, and diminished adult eclosion and female ratio in the treated lines. Sterility reached 100% above 5 Gy levels except for the cross U´I that resulted in 55.2±21.3% sterility at 6 Gy, while complete infecundity in all flies was induced by 10 Gy. Males were readily radiosterilized than the females. Dose mortality (LD50) and sterility (SD50) responses of the test insects were determined 53.03 Gy and 4.34 Gy, respectively. Results are promising in connection with a sterile insect technique (SIT) for this pest species. Key words: Musca domestica; gamma radiation; pupal treatment; oviposition; sterility; immature mortality; adult eclosion; female ratio 1Professor, Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh. 2Lecturer of Biology, Al-Haj Mockbul Hossain University College, Kaderabad Housing, Katasur, Mohammadpur, Dhaka-1207 Name of corresponding author: Dr. M. Saiful Islam Email: [email protected]

87

Study of Artificial Breeding, Growth and Biochemical Genetic Analysis of Endangered Local Sarpunti, Puntius Sarana (Hamilton) for its Revival

Imran Parvez1 and Md. Mukhlesur Rahman Khan2

To revive the endangered local sarpunti Puntius sarana, artificial breeding within and between two different stocks namely Sukhair haor of Sunamganj and the Kangsha River of Netrokona district were conducted. Three breeding lines namely line-1, line-2 and line-3 (Sukhair x Sukhair, Sukhair x Kangsha and Kangsha x Kangsha respectively) having three replications of each were performed with pituitary gland extract (PG) at the doses of 6.5 mg/kg body weight and the breeding parameters in terms of ovulation, fertilization and hatching rate were studied. The growth performances and survival rate of the larvae of three lines (named as SS, SK and KK) were also studied for 35 days in glass aquaria. Offspring of the best performing line was selected for the genetic comparison with their parents using horizontal starch gel electrophoresis. The genetic variations were analyzed using ten enzymes (ADH, EST, G3PDH, G6PDH, GPI, IDHP, LDH, MDH, PGM and SDH) in two buffer systems (CA 6.1 and TC-1). Induction of artificial breeding between Sukhair and Sukhair (line-1) showed the better result in terms of ovulation (83.75%), fertilization (74.25%) and hatching rate (51.18%) compared to other two lines. Higher growth and survival rate were observed in larvae of line-2 (SK), and hence they were forwarded to genetic study. In genetic study, fifteen presumptive gene loci were observed in which the mean proportion of polymorphic loci were 26.67%, 26.67% and 13.33% for broods of Sukhair, the offspring of line-2 and broods of Kangsha respectively. The highest mean number of allele per locus and mean proportion of heterozygous loci was observed in the line-2 (SK) (1.33 and 6.67% respectively). The UPGMA dendrogram (Figure 1) based on Nei’s (1972) genetic distance revealed that the offspring of line-2 and Kangsha population made one cluster (D=0.001) and separated from Sukhair by the genetic distance of 0.0183. The results suggested that the offspring of line-2 (SK) of P. sarana performed better growth and survival and higher genetic variations, which indicated the genetic improvement of P. sarana and if we take immediate action by following this protocol, we can revive this species from the threatened situation and can make available them in nature.

Figure 1. UPGMA dendrogram based on Nei’s (1972) genetic distance showing the genetic differentiation among parents and their offspring (Line-2). Key word: Endangered, Puntius sarana, Electrophoresis, Genetic variation, Revival 1 Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, 2 Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh- 2202

Name of corresponding author: Imran Parvez; Email: [email protected].

Sukhair

Kangsha

Line-2 (SK) 0.0183

0.001

0.000 0.0183

Genetic distance (D)

88

Genetic and Biochemical Testing for Hereditary Breast and Ovarian Cancer

Jalaluddin Bhuiyan a, PhD, DABCC, FACB and Muhammad Faiyaz-ul-Haque a, PhD.

The prevalence of breast cancer stands second to lung cancer and account for high morbidity and mortality rate throughout the world. Breast cancer is very common in women. The identification of BRCA1 and 2 mutations responsible for hereditary breast cancer has a significant value towards clinical management and counseling of the patient. Cancer that is detected early can potentially be cured when the tumor is small enough to be completely removed surgically. Unfortunately, most breast cancers do not produce any symptoms until the tumors are either too large to be removed surgically or cancerous cells have already metastasized. Hence there is need to detect cancer at an early stage. The detection of causative mutations in the BRCA1 and 2 genes in the afflicted families serve several clinical purposes in such high prevalent disease. Tumor markers are mainly used to predict the staging, prognosis, response to therapy, and monitoring the treatment and course of the disease. High quality biochemical tests monitoring the ovarian function by extremely sensitive immunoassay and tandem mass spectrometry can provide accurate assessment for hormone therapy. Data on BRCA1 and 2 involvements in breast cancer from the Sub-Continent is very scarce, therefore, it is important to generate and establish a data base from local patient sample as well as from the control cohort. Identifying the genetic and biochemical etiologies in these disorders will provide insight into our understanding of the highly complex genetic and molecular basis of breast cancer. a Sections of Clinical Biochemistry and Molecular Genetic, Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Kingdom of Saudi Arabia.

89

A Comparative Study of the Genes related to Long-term potentiation (LTP) and Memory development

Shahani Noor1, Jesmin2

The molecular mechanisms underlying the memory development process is highly complicated and still poorly understood. A number of studies have already revealed that long-term potentiation (LTP), is critical for memory development in mammalian brain. It has also been observed that high level expression of a number of genes including MAPK, NAMDA, RAS, RAF, CAMK2A, EGFR, PKC, PKA and FOXP2 are someway related to the memory development and learning process. However, the exact molecular mechanisms and the roles played by these genes have yet to be definitely assigned.

In order to develop a better understanding of the memory development process in human, a comparative genomic study of these genes has been carried out. The Human genomic data, in and around of these targeted genes have been compared mainly with the recently published Chimpanzee genomic data for the various markers such as polymorphisms (SNPs), EST, CpG islands and contigs. Homology study along the genomes of other distantly related species has shown that the codings of these genes are very much conserved within species. It has been found that the Rafp share high degree of sequence homology (~60%-87%) with the Kinase Suppressor of Ras (KSR). KSR is one of the most enigmatic scaffolds identified for Ras/ERK signaling. Multiple sequence alignment and phylogeny analysis data have indicated that Raf homologs are highly conserved in a wide range of species including Rhesus monkey, Gorilla, Dog, Zebra fish, Mouse, Mosquito, Fruit fly and in Honeybee. However, the Raf homologs data of Human and Chimp (sharing ~ 68% homology with each other) have shown to diverge significantly from the other related species. The detailed genomic analysis data revealed that the critical cystein residues of cystein-rich CA3 region and the putative kinase domain were highly conserved within all the species analyzed. It has also been found that the ERK1/2 binding site within KSR, is a FxFP motif within the serine/threonine-rich CA4 region of the protein, is absent in Human and Chimp Rafp homologs but well conserved in other distantly related species.

1,Department of Genetic Engineering and Biotechnology, University of Dhaka, Bangladesh 2Department of Genetic Engineering and Biotechnology, University of Dhaka, Bangladesh Name of corresponding author: Dr. Jesmin, Email: [email protected], Tel: 9661920, Ext: 7819.

90

Expression, Identification and purification of Cry protein from entomopathogenic Bacterium Bacilus thuringiensis israelensis

Kazi Asraful Alam1

Expression and identification of putative Cry proteins of the entomopathogenic bacterium

Bacillus thuringiensis israelensis have been documented during 24, 48, 72 and 96 hours of

bacterial culture both at 30 and 37°C. Putative Cry 11A and Cyt A, Cry4B and Cry4A have been

recorded to be early, mid and late expressing proteins respectively. No difference on the level of

expression of Cry proteins has been observed between 30 and 37°C. The mosquitocidal proteins

were found to be very thermo labile. Heat-treated proteins were found to loose the

mosquitocidal efficacy against the larvae of Aedes aegypti mosquito.

Key words: Cry protein, Bti 1 MSc 1st term, Biotechnology and Genetic Engineering Discipline, Khulna University.

91

Analysis of 16S rRNA and Dehalogenase Genes of Microbial Consortia Involved in Dechlorination of Chloroethenes

K.H.M.N.H. Nazir1, F. Okamoto2, H. Hashimoto2, T. Futagami2, M. Goto2

and K. Furukawa2 Among the groundwater pollutants, chloroethenes were considered as the most serious worldwide. The reductive dechlorination of these compounds by dehalorespiring bacteria in the anaerobic environment is significantly important from the viewpoint of bio-remediation. The research work was conducted in the laboratory of Applied Microbiology, Kyushu University, Japan. We investigated the microbial involvement in microbial consortia by PCR-DGGE, 16S rDNA analyses and detection of the dehalogenase genes. After complete dechlorination checked by gas chromatography, total DNA was extracted from the consortia, PCR-DGGE analyses targeting for 16S rDNA V3 region was done. The results revealed that several microorganisms constitute the consortia. From the extracted genome, the 16S rDNA clone libraries were constructed by using three sets of primer pairs. The first two (66S/180C and 793S/946C) were targeting for Dehalococcoides spp., and the third one (8S/1492C) was targeting for all bacteria of the consortia. After sequencing of 30 clones from each group, we found that two clones termed Nazir-1 and Nazir-2, were closely related with chloroethene dechlorinating Sulfurospirillum halorespirans strain PCE-M2 (98.24%) and with Dehalococcoides sp. strain BAV1 and FL2 (98.45%), respectively. Using specific primers and sequencing, we also detected four dehalogenase genes such as bvcA, vcrA, rdhA3 and rdhA8 in the consortia by PCR. The results suggesting that several microorganisms found in this study have been enriched in the consortia and these are directly or indirectly involved in effective and complete dechlorination of chloroethenes. Key words: Microbial consortia, bioremediation, chloroethene, DGGE, 16S rDNA, dehalogenase genes

1Department of Microbiology and Hygiene, Bangladesh Agricultrual University, Mymensingh-2202, Bangladesh. 2Laboratory of Applied Microbiology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan Name of the corresponding author: KHM Nazmul Hussain Nazir Email: [email protected] or [email protected] or [email protected] Fax: +8809155810

92

Salinity and Drought Resistant Genes in Potato: Cloning, Expression, Transformation and Variety Development

Nasiruddin KMa, R Begum1, S K Sopory2 and A K Mattoo3

Salinity and drought are the universal common hurdles for increasing the land cropped area of potato particularly in Bangladesh and India. Coastal area, 15% of total cultivable land, remain fallow due to salinity problem. Similarly, due to the lack of irrigation facilities cultivable land remain barren in dry period. Potato germplasm including some released varieties were collected and maintained on the Farm and in the USDA Biotechnology Lab in vitro. The meristem cultured apical and nodal explants were grown on MS medium supplemented with 3% sucrose, 5 mg/l BAP and 500 mg/l CCC. Plantlets were regenerated from leaf disc and nodal segment with subsequent microtuber formation with different growth regulators. The molecular characterization by RAPD and isozyme revealed the diversity and relatedness of the genotypes. Experiments were conducted to screen the genotypes against different levels of drought and salinity condition in vitro, in the pot and in the field. Differential responses were found among the genotypes. Salinity and drought resistant gene glyoxalase I isolated from Brassica is inserted in universal plant transformation vector pB1121 which is transformed into Agrobacterium tumefaciens. Plantlets were regenerated after cocultivation on selection medium to get transgenic potato plants (47.65%) for possible resistance against salinity and drought. Genomic DNA was isolated from the microplants grown on selection medium and PCR was performed where 85.25% of the regenerated plants gave the PCR positive as trangenecity confirmation test. Leaf discs of PCR positive plants along with wild plants were placed on MS plates with variable salinity and drought gradients as a bioassay test. Greenness and normality in growth of the leaves (78.35%) from transgenic plants revealed that those gained tolerance over the abnormally grown yellowish wild leaf discs (87.45%). However, Southern, Northern and Western blotting analyses be done for conclusive and reproducible results. aDepartments of Biotechnology ([email protected]), 1Agril Statistics, Bangladesh Agricultural University, Mymensingh, Bangladesh. 2ICGEB, Aruna Asaf Ali Marg, New Delhi, India, 3USDA Vegetable Lab, Beltsville Agricultural Research Centre, Beltsville, USA

93

Coastal Rice landraces of Bangladesh: DNA Fingerprinting and Potential as Donor for Salt Tolerance Traits

Laisa Ahmed Lisa1, Sazzadur Rahman2 and Zeba Islam Seraj3

Farmers in the saline coastal belt of Bangladesh still grow traditional landraces in preference to the modern varieties particularly in the dry winter or Boro season when the salinity level rises high. Characterization of these landraces can suggest how they survive in adverse soils and indicate suitable target genes for transfer to modern rice varieties.

Twenty Oryza sativa L. landraces (LRs) from the saline prone coastal zones of Bangladesh were analyzed with 60 evenly distributed rice microsatellite DNA markers. All IRRI and BRRI released modern varieties clustered into two groups different from the three groups containing the photoperiod sensitive landraces. The photoperiod insensitive landraces from the mildly saline coast of the mid northeast clustered into a separate group. The internationally well-known standard for saline tolerant rice, Pokkali did not group with any of the 20 landraces. Thirty six landraces from the southwest coast were subjected to morphological observations in non-saline soil and screening with 100 mM NaCl salinity stress in hydroponics at seedling stages was conducted. Seven landraces from the highly saline southwest were identified as the most tolerant. UPGMA clustering grouped all 7 LRs with the tolerant control Pokkali.

Two LRs (Horkuch & Jamainaru) out of the latter eight, a modern variety BRRIdhan41 known to be moderately tolerant and the controls were further screened up to maturity under four different stress environments. Irrespective of the seasons and stress conditions, among the landraces, Horkuch performed best of all compared to the tolerant control Pokkali. Minimal change in growth parameters, low Na+/K+ ratio in flag leaves and comparatively lower percent reduction in yield were indicative of its better performance. Good partitioning of Na in the older leaves and maintenance of comparatively higher levels of beneficial Ca and Mg in the flag leaves contributed to its good yield performance and salt tolerance. These results indicate that Horkuch can be a potential donor alternate to Pokkali for salt tolerance in breeding programmes.

Gene expression studies showed that both Horkuch and Pokkali show significant induction in LEA3 gene expression under salt stress, which indicates that both these landraces synthesize a protein known to cause stabilization of other proteins at low water potential. Increase in PTO kinase interactor expression in Horkuch is probably due to general oxidative stress.

1Department of Biochemistry and Molecular Biology, University of Rajshahi 2Bangladesh Rice Research Institute, Gazipur, Dhaka 3Department of Biochemistry and Molecular Biology, University of Dhaka

94

Genetic Structure of Wild and Hatchery Populations of Indian Major Carp, Mrigal (Cirrhinus Cirrhosus) In Bangladesh.

Hasanat, M A

The present study was aimed to reveal the genetic structure of wild and hatchery populations of one of the Indian major carps, mrigal (Cirrhinus cirrhosus) in Bangladesh. Two popular molecular markers (allozyme and DNA microsatellite) were chosen for this study. For allozyme electrophoresis, mrigal were sampled from three main rivers (the Halda, the Jamuna and the Padma), one branch river of the Padma (Gorai) and three hatchery sources (Brahmaputra hatchery, Mymensingh; Raipur hatchery, Laxmipur and Sonali hatchery, Jessore) covering a wide geographical distribution of this species. Fifteen different enzymes (AAT, ADH, AK, EST, EST-D, G3PDH, G6PDH, GPI, HK, LDH, MDH, ME, PGDH, PGM and SOD) were put on trial in two tissue systems (muscle and liver) to reveal the best resolution for routine analysis of genetic variation. Muscle tissue provided better resolution compared to liver tissue. Out of fifteen, only four enzymes (EST, G3PDH, G6PDH and GPI) were proved feasible for identifying polymorphism (P95) in all mrigal populations. The average observed heterozygosity (Ho) and expected heterozygosity (He) in the river populations were comparatively higher than those of hatchery populations. Among all the populations only the Padma-2 (Gorai, branch of the Padma river) and Raipur hatchery populations maintained Hardy-Weinberg equilibrium across all loci. The minimum genetic distance (D=0.001) was observed between the Halda and Raipur hatchery populations, while the maximum genetic distance (0.015) was found between Padma-1 (the Padma) and Sonali hatchery populations. Among the river populations maximum genetic distance (0.012) was observed between Padma-1 and Padma-2 and minimum genetic distance (0.003) between Padma-2 and Jamuna populations. Dendrogram based on UPGMA analysis of allozyme data from seven populations resulted that Padma-1 population was completely isolated from all other populations. Among other six populations Halda along with Raipur hatchery populations was in one cluster and remaining four populations were in the other cluster. These four populations again were separated into two sub-clusters, Brahmaputra hatchery and Sonali hatchery in one sub-cluster and Jamuna and Padma-2 in another sub-cluster. The use of microsatellite DNA marker was verified for discriminating all the populations, used in allozyme experiment except Padma-1 with a representative sample. A total of five microsatellite markers (MFW1, MFW2, MFW17, Barb54 and Bgon22) were applied. Out of five microsatellite loci, three (MFW2, MFW17 and Bgon22) were found to be polymorphic (P95) in all populations. The average observed heterozygosity (Ho) in the river populations was higher than that of hatchery populations but the expected average heterozygosity (He) of river populations was lower than that of hatchery populations in microsatellite analysis. The Padma-2 population maintained Hardy-Weinberg equilibrium across all loci in microsatellite data analysis but the other five populations were in disequilibrium at least in one locus. The lowest genetic distance (0.001) was obtained between Halda and Raipur hatchery populations while highest genetic distance (0.086) between Jamuna and Raipur hatchery populations in this experiment. Dendrogram based on UPGMA analysis of microsatellite data from six populations resulted in two major clusters: Halda population along with Raipur hatchery population was in one cluster and remaining four populations were in the other cluster which was again separated in two sub-clusters similar to the sub-clusters obtained from allozyme data. The clear-cut assessment of genetic variation across the natural distribution of mrigal requires analysis using a larger sample size with more enzymes and primers for allozyme and DNA microsatellite analysis respectively. Individuals from different geographical locations may expose new alleles at polymorphic loci and probably identify polymorphism in more loci, which was detected as monomorphic in this study.

95

Molecular Characterization of Soybean [Glycine Max (L.) Merr. ] Cultivars Using Microsatellite Markers for Plant Variety Protection

M.A.N.Nazim-ud-Dowla1*, Md. Rezwan Molla1, Mohammad Nazrul Islam2, Md.

Shefatur Rahman1, Md. Samsul Alam2 and Lutfur Rahman1

Soybean [Glycine max (L.) Merr. ] cultivars are described for the purpose of Plant Variety Protection (PVP) by standard pigmentation and morphological traits. However, many commercial soybeans developed from a limited number of elite lines and are often indistinguishable based on these traits. A system based on DNA markers particularly Simple Sequence Repeats or SSR markers could provide unique DNA profiles. Microsatellite allele size profiling technology can be readily used to size SSR alleles from any organism. The purpose of the work presented here was to select and evaluate a small set of three SSR markers with maximum reliability and repeatability that would provide a high level of discriminatory power to distinguish soybean genotypes. A total of three SSR primers (SATT 5, SATT 9 and SATT 141) were used to amplify genomic DNA of the six soybean varieties, BAU 5, BAU 6, BARI soybean 4, BARI soybean 5, Davis and Shohag. All the six varieties were distinguished by the three primers. Among the three primers, SATT 5 was found to be the most informative one (PIC= 0.78) as four varieties (BARI soybean 4, BARI soybean 5, BAU 5 and BAU 6) were identified at this locus. A total of seven variety specific alleles were found, these are, SATT 5/146 for BAU 5; SATT 5/155, SATT 9/144 and SATT 141/176 for BAU 6; SATT 5/144 for BARI soybean 4; SATT 5/149 for BARI soybean 5 and SATT 9/204 for Davis. Key words: Soybean, Variety Identification, SSR. 1Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymenisingh; 2Department of Fisheries Biology and Genetics, BAU, Mymensingh; *Corresponding Author: ([email protected]) Studied under the funding support of the DANIDA for Seed Industry Development of the Ministry of Agriculture.

96

Elevated Serum Levels of Heat Shock Proteins in Small Cell Lung Cancer

Md Abdul Khaleque1*, Patrick Ma2, Stuart K. Calderwood1, Ravi Salgia2 and Ajit Bharti2 Heat Shock Protein (HSP) was first discovered as cohort of proteins that are powerfully induced by heat shock and other chemical and physical stresses. The HSP have been subsequently characterized as molecular chaperones, proteins which have in common the property of modifying the structures and interactions of other proteins. Induction of HSP27, 70, 90 and 110 become the dominantly expressed proteins after stress. In addition to the HSP induced by heat, cells also contain a large number of constitutively expressed HSP. Many tumor types have been reported to contain high concentrations of heat shock proteins of the HSP27, HSP70 and HSP90 families. The role of HSP in tumor development may be related to their function in the development of tolerance to stress. Elevated HSP expression has been conjectured to participate in tumor pathogenesis through the ability of individual HSP to block the pathways of apoptosis and permit malignant cells to arise despite the triggering of apoptotic signals. HSP expression is likewise thought to afford protection of cancer cells from treatments such as chemotherapy and hyperthermia by thwarting the pro-apoptotic influence of these modalities. HSP have been regarded as intracellular proteins. However, enough evidence has been shown that under certain circumstance they are released from cells. We have determined the circulating level of HSP70 and HSP27 in small cell lung cancer representing limited disease (LD), extensive disease (ED), no evidence of disease post therapy (NED) and relapsed (RL). Our data indicates higher level of circulating HSP70 and HSP27 in different groups of patients compared to normal healthy individual. The group representing ED and RL showed significant level of change. While HSP70 level remains consistently higher in all patients within a group, patient to patient variation was observed in the serum level of HSP27. Key words: Heat shock protein, small cell lung cancer, HSP27, HSP70 1Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA

2Center for Molecular Stress Response, Department of Medicine, Boston University, Boston, MA 02118, USA

*Present address: Zymed Division, Invitrogen Corporation, Camarillo, CA 93012, USA

Contact e-mail: [email protected]

97

Development of Novel Multiplex Reverse Transcriptase-PCR Assays for Rapid Detection of Arboviruses

M. A. Islam1, 4 & 5, S. Inoue1, A. H. Khan1 , T. Nabeshima1, A. Jittmittraphap2, Aryati3, N.

Talemaitoga1, Y. Jin1, and K. Morita1, 4

This study was designed to develop a highly specific, sensitive and cost-effective molecular diagnostic assay for rapid detection of Dengue virus sero-types (DEN 1-4), Chikungunya virus (CHIK) , Japanese encephalitis virus (JEV) and West Nile viruses (WNV) from the clinical sample (serum) , infectious culture fluid (ICF) and mosquitoes, by a single-tube-single-step multiplex RT-PCR (mRT-PCR I & II) assays. Specificity and sensitivity of the mRT-PCRs and uniplex RT-PCR (uRT-PCR) have been compared and evaluated using 6 different cross combinations of Reverse transcriptases (RT-Ace and AMV-RT) and DNA-polymerases (LA-Taq, rTaq and Tth). Of the 6 combinations, the AMV-RT and LA Taq was found superior in terms of sensitivity and specificity compare to other. Genome detection limit of mRT-PCR I for DEN-1, 1; DEN-2, 20 ; DEN-3, 0.1 ; DEN-4, 10 and CHIK was 10 FFU (focus forming unit) and the mRT-PCR II for JEV and WNV was 10 and 1 FFU, respectively. The primers designed specifically for these mRT-PCRs did not show cross-reactivity within the sero-types of Flavi and Alpha viruses. Therefore, these two mRT PCRs assay could be used as a highly sensitive, reliable, rapid and cost-effective diagnostic tools for the diagnosis and molecular epidemiological surveillance of the viruses. Key words: Multiplex RT-PCR, Rapid Detection, Arbovirus 1 Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan, 2 Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol , University, Thailand , 3 Department of Clinical Pathology, Dr. Soetomo Hospital, Faculty of Medicine, Tropical Disease Center, Airlangga University, Surabaya, Indonesia , 4 Core Research for Evolutional Science and Technology (CREST), Japan, 5 Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Name of corresponding author: Prof. Dr. Md. Alimul Islam E.mail: [email protected] Fax: +88091-55810

98

Antibiotic Susceptibility and Molecular Characterization of Salmonella Paratyphi A Isolated in Bangladesh

K. A. Talukder1, M. A. Zaman2, Z. Islam1, M. Aslam1, A. S. Mondol1, I. J. Azmi1, M. A.

Islam1 and A.K.A. Chowdhury2

Enteric fever, comprising both typhoid and paratyphoid fevers, continues to be a major public health problem even after the development and advent of newer antimicrobial drugs. Salmonella enterica serovar Paratyphi A is the second most common cause of enteric fever after S. Typhi in developing countries including Bangladesh. Till date there are no published data which can reflect the present status of the prevalence susceptibility pattern and genetic properties of S. Paratyphi A strains in Bangladesh. The major objective of this study was to characterize Salmonella Paratyphi A strains using phenotypic and genotypic traits. A total of 524 strains of Salmonella Paratyphi A were isolated at Clinical Microbiology Laboratory from patients attending the Dhaka treatment center of ICDDR,B as well as from patients referred from other clinics and hospitals in Dhaka between January 1999 and June 2004. Of these, 31 strains were characterized extensively using serotyping, antibiotic resistance analysis, plasmid profile analysis and pulsed-field gel electrophoresis (PFGE) and DNA sequencing was performed to investigate the resistance mechanism against fluroquinolone antibiotics. Among the 31 strains, 32% (n=10) were resistant to nalidixic acid. Strains that showed reduced susceptibility to ciprofloxacin by disk diffusion method, had elevated MIC value (0.5µg/ml). Analysis for mutation in the gyrA and parC genes in the QRDR by sequencing revealed a point mutation in the gyrA gene at codon 83 (TCC to TTC), which substitutes phenylalanine for serine whereas no mutations were found in parC gene. Of 31 strains, 16 (52%) S. Paratyphi A strains harbored 35 MDa middle-ranged plasmid which were found to be non-conjugative. Pulse-field gel electrophoresis (PFGE) showed that most of the strains (83%) were clonal regardless of antimicrobial sensitivity patterns. The study emphasizes further extensive characterization and epidemiological investigation of Salmonella Paratyphi A which will aid in undertaking strategy to combat antimicrobial resistance in the species and redefining the treatment strategy with conventional, effective antimicrobial agents in the face of the emergence of strains with reduced susceptibility to fluoroquinolone group of antibiotics. Keywords: Enteric fever; pulsed-field gel electrophoresis; fluoroquinolone; QRDR; DNA sequencing; point mutation 1ICDDR,B, GPO Box 128, Dhaka-1000, Bangladesh 2Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Ramna, Dhaka-1000, Bangladesh Name of the corresponding author: K. A. Talukder E mail: [email protected]

99

Need for production of Pharmaceutical and Industrial Protein- A Bangladesh Prospectus

1Md. Ashraful Islam Bhuiya, 2Dr. Muhammad Hafizur Rahman,

1Sougatul Islam, 1Nazlee Sharmeen, 1Tasnuva Sarowar

Production of pharmaceutical and industrial protein is one of the major branches of Industrial Biotechnology and its demand and consumption are growing dramatically. In developed countries different companies have already established such protein industries investing large amount of capital. It has a big worldwide market. Unfortunately there is no biotechnology-based industry in Bangladesh while it has a great market here. So this is the time to take initiatives to establish industries based on biotechnology in our country. Insulin: There are a great number of consumers of insulin in all over the world as well as in Bangladesh. Estimation of Diabetic patients in Bangladesh: Patients registered with:

• BIRDEM = 0.35 Million • NHN = 0.11 Million • Affiliated Association = 0.15 Million

Total patient = 0.61 Million x 6 = 3.66 Million. DAB is taking care of approx. 15-20% of the estimated diabetic patient in Bangladesh. In Bangladesh Insulin market is occupied 98% of Novo Nordisk, 1.1% by Lily, 0.9% of Santofi Aventis. Present condition in Bangladesh of Biotech Industries: There is no pharmaceutical and other industrial protein producing company. Only some vaccines are produced here, which have a healthcare value but no significant economic value. Pharmaceutical companies import raw materials of insulin, antibiotics and perform some pre-selling test like the test of their activity, stability and do some modifications if needed. Then they pack them and sell to market. To produce protein industrially after completing whole procedure (from fermentation to market) it takes several years and initially it takes a large amount of money. But industrialists in our country do not want to take risk initially to invest this large amount of money to establish such industries. It may be due to lack of interest and proper knowledge. They want to invest money in a way from which benefits come easily and in short time. So initiatives should be taken immediately to solve this problem by scientists, government as well as by renowned industrialists of our country. 1 Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka-1000 2Bangladesh Institute of Research and Rehabilitation for Diabetes, Endocrine and Metabolic Disorders (BIRDEM), 122 Kazi Nazrul Islam Avenue, Dhaka-1000.

100

Genetic Diversity of Bitter Gourd Germplasm of Bangladesh

M.A. Rahman1, M.G. Rabbani2, G.J. Ahammed3, J. Akhter4 and E.J. Garvey5

Genetic diversity of a number of bitter gourd accessions collected from different parts of Bangladesh was evaluated based on phenotypic traits, peroxidase activity and RAPD markers. High heritability with genotypic and phenotypic coefficient of variation were observed for vine length at final harvest, fruit length, average fruit weight and yield, indicating additive gene effects of these traits. Correlation coefficient study indicated that yield per plant had highly significant positive correlation with average fruit weight, fruit length, number of fruit per plant both in genotypic and phenotypic levels. Genotypic path coefficient analysis showed that fruit length had maximum direct effect on yield per plant, whereas average fruit weight had maximum direct effect on yield per plant. Genetic divergence utilizing multivariate analysis of phenotypic data grouped 38 accessions of bitter gourd into six clusters, showing no relationship between genetic and geographic distribution. Peroxidase activity patterns suggested four zymotypes in bitter gourd. RAPD profiling of twenty selected accessions using 8 decamer random primers generated 110 distinguished bands, 87 of which were polymorphic (79.09%). The highest inter-accession similarity index (Sij) was found between MC 172 vs. MC 018 (95.79%) with a gene diversity (h) value across all loci as 0.26. The UPGMA dendrogram based on genetic distance suggested 2 main clustering of 20 selected bitter gourd germplasm – 2 accessions (MC 172 and MC 018) forming cluster I, and the rest 18 accessions forming cluster II, where 16 accessions were grouped together in sub-cluster (I), while 2 accessions (MC083 and MC105) formed sub-cluster II.

Key words: Bitter gourd, genetic diversity, peroxidase isozyme, RAPD, 1 Associate Professor, Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202 2 Professor, Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202 3 Assistant Director, BADC 4 MS Student, Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202 5 Plant Exchange Office, USDA/ARS, BARC-West, Beltsville, Maryland MD 20705, USA Name of corresponding author: Dr. Md. Azizur Rahman Email: [email protected]; [email protected] Telephone +88091 55695-7 ext 2440, 0171105 8453 (cell) Fax: 880-91-55810

101

Morphological, Physiological and Molecular characterization of Phomopsis vexans isolates of Eggplant (Solanum melongena) of

Bangladesh

M.M. Islam1 and M.B. Meah1

Phomopsis vexans isolates from eggplant (Solanum melongena L.) of Bangladesh were examined using traditional mycological characteristics and molecular methods. Variation exists among the isolates of Phomopsis vexans collected from core eggplant growing areas of Bangladesh covering two types of farm having two ecosystems and the isolates were grouped into five distinct groups based on their cultural properties. The pathogen grew best at 250C, under 12/12h cycle light and pH 5.5 levels of culture media. Number of spores per ml was also maximum at 250C temperature, 12/12h cycle light and pH 5.5. Brinjal Fruit Extract Agar media supported the maximum growth and sporulation. Two types of spores á and â were detected in Phomopsis vexans. Five isolates had variation in growth and sporulation under different levels of temperature, light, culture media and pH. á and â conidia varied in size among five isolates. Molecular markers for P. vexans isolates were developed and genomic DNA were analyzed by using the polymerase chain reaction (PCR)- variable number of tandem repeats (VNTR) and amplified fragment length polymorphism (AFLP) techniques. The MR -20 primers amplified PCR products for all isolates studied. Gel electrophoresis of undigested PCR products produced various concentrations including 12.5 - 50 ng/µl. Digestion of these PCR products with enzyme Taq polymerase resulted in distinct bands to identification of P. vexans. The banding patterns of P. vexans isolates were separated into five groups after digestion with Taq polymerase. From this molecular study, 44 isolates were identified as Phomopsis vexans and grouped into five different categories. In addition to distinguish inter-specific and intra-specific variability, molecular characterization allowed the detection of differences among the isolates within the same variety and among the areas.

1 IPM Lab, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh -2202, Bangladesh

102

Isolation of Plasmid DNA from E. coli (JM109 & BL21DE3) for the Preparation of Recombinant DNA Cell

M.B. Hossain1, J.W. Choi2 and K.S. Ryu2

Eschaeria coli (E. coli) used for plasmid DNA isolation to produce recombinant DNA using different enzyme, primers and vector. Plasmid DNA isolation, electrophoresis, competent cell preparation, transformed cell, endonuclease enzyme restriction digestion, elution and ligase were followed by standard procedures. E. coli (JM109 & BL21DE3), pT7-7 ligase vector, 5V plasmid insert vector, NdeI and HindIII primer were used for endonuclease enzyme restriction digestion. Endonuclease enzyme restriction samples were prepared for elution to confirm the production of recombinant DNA sample. Recombinant DNA samples were observed from the studied results with compared the standard sample. Some samples were more number of base pair (bp) and some samples lower base pair recombinant cell comparing to standard sample under this study. From these results it may be concluded that we can produce a wide range antibiotic producing beneficial microorganisms which can help to induce disease resistant capacity in rhizosphere soil. Keywords: E. coli, plasmid DNA, vector, primer, recombinant cell, molecular technique 1Soil Microbiology Laboratory, Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA), P.O. Box 4, Mymensingh 2200, Bangladesh. 2Lab. of Soil Science, Department of Food and Bioenvironmental Science, College of Life & Environment, Daegu University, Kyongsan, Kyongbuk, Korea 712-714, Republic of Korea Name of the corresponding author: Md. Belal Hossain E-mail: [email protected] Fax: +82 053850 6759

103

Immune Cascade of Spodoptera Litura: Cloning, Expression and Characterization of Prophenol Oxidase Activating Enzyme 1(Ppael)

M. E. Hoque1, R. Rajagopal2, Bindiya Sachdev2 and R. K. Bhatnagar2

Prophenol oxidase (PPO) cascade is one of the major insect immune component for combating invasion of a microbe or healing incidental wounds. The PPO cascade is triggered by the activation of prophenol oxidase activating serine protease. We have isolated, cloned and expressed prophenol oxidase activating enzyme1 (ppae 1) from polyphagous pest Spodoptera litura. The structure of matured polypeptide is reminiscent of other lepidopteran PPAE in having a signal peptide, propeptide and catalytically active polypeptide. It shares 64% identity and 12% similarity with PPAE 1 of Manduca sexta. The cDNA has been expressed in Sf-21 cells using baculovirus expression vector. Fractionation of expressing Sf-21 cells revealed its expression in the membranes. The recombinant protein was solubilised from membranes and purified by Ni+2-NTA affinity chromatography. The purified enzyme is catalytically active on chromogenic substrate, activated recombinantly expressed prophenol oxidase (PPO) of S. litura and was inhibited by aprotenin.

Keywords: PPAE; PPO; cloning; expression; Spodoptera litura

1Assistant Professor, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

2Insect Resistance Lab, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi – 110067, India

104

Study on Interrelationship among 10 Citrus Fruits Using RAPD

M. A. Maya, M. G. Rabbani* and E. J. Garvey1

Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

An experiment was conducted to study the interrelationship among 10 Citrus fruits using RAPD. Seven decamer primers were selected for the present study after screening of 19 decamer primers which gave 90 clear and bright fragments. Out of these, 85 fragments were considered as polymorphic. The proportion of polimorphic loci was 94.44% and gene diversity (h) value was found 0.30 across all loci. The highest (84.01 %) and the lowest (27.43 %) inter-species similarity indices (Sij) were observed between C. jambhiri vs C. variegata and Poncirus trifoliata vs C. assamensis species pairs, respectively. The UPGMA dendrogram based on genetic distance segregated 10 Citrus fruits into 2 main clusters where Poncirus trifoliata alone formed 1 cluster and the rest 9 species grouped together into another cluster. C. jambhiri and C. variegata were very close to each other with the lowest genetic distance (0.14). On the other hand, Poncirus trifoliata and C. assamensis were more distant to each other with the highest genetic distance (1.00). The RAPD analysis appeared to be a rapid and reliable method for the estimation of variability and interrelationship among Citrus fruits which could be utilized by the breeders for further improvement of Citrus fruits. 1Plant Exchange Office, USDA/ARS, BARC-West, Beltsville, Maryland MD 20705, USA * Author for correspondence; Email: [email protected]

105

Molecular Characterization of Sweet Gourd Using RAPD

E.H.M.S. Rahaman1, M.G. Rabbani2 and E.J. Garvey3

Sweet gourd (Cucurbita moschata Duch. Ex. Poir.) is one of the popular cucurbitaceous vegetables in with highest storability among all the cucurbits. Sweet gourd is also rich carbohydrates, soluble fiber, â-carotene and vitamin E. Due to its good storage quality, nutritional status, reasonable market price and year round availability; it has a great demand in Bangladesh. There is lot of variability among the existing germplasm of sweet gourd in Bangladesh. There is no report of molecular characterization of sweet in Bangladesh. The present experiment was conducted during the period from December 2004 to March 2005 to characterize 81 sweet gourd germplasm collected from different parts of Bangladesh using RAPD as molecular markers. After screening of 39 decamer primers from kits A, B, C, K and L of Operon Technologies, USA; five (primers OPK 12, OPK 16, OPL 07, OPL 14 and OPL 18 ) were used for molecular characterization of sweet gourd germplasm that produced 50 RAPD loci, of which 58% was polymorphic. The highest pair wise genetic distance was 0.446, between the accessions of CM041 and CM092. The mean gene diversity among all the germplasm was 0.184. The UPGMA dendrogram based on Nei’s genetic diversity demonstrated 8 clusters. Clustering of the germplasm based on morphological characters did not match with the clusters obtained from RAPD analysis. In case of RAPD analysis, no relationship was noticed between the genetic diversity and geographical distribution of the sweet gourd germplasm. Key words: Sweet gourd, Molecular characterization, RAPD, Dendogram 1 Senior Scientific Officer, TCRC, BARI, Joydebpur, Gazipur 2 Professor, Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202 3Plant Exchange Office, USDA/ARS, BARC-West, Beltsville, Maryland MD 20705, USA Name of corresponding author: Professor Dr. Md. Golam Rabbani Email: [email protected] [email protected] Cell: 01711885790 Fax: 880-91-55810

106

REP-PCR fingerprint of Lentil (Lens culinaris) Rhizobia isolated from

Bangladesh.

M.H. Rashid1, M. A. Sattar1 and J.P.W. Young2.

Rhizobia are the soil bacteria having the ability to induce nodules on lentil roots. Select highly effective strains is an important objective in bio-logical nitrogen fixation research because bacteria used for inoculants have to compete with indigenous flora for survival and establishment. An attempt has been made to characterize some Bangladeshi rhizobial strains using sensitive and reliable method to detect and quantify introduced rhizobia as bio-fertilizer and asses their nitrogen fixation capacity under laboratory condition. The experiment was conducted at the university of York, UK and Soil microbiology laboratory, Bangladesh Institute of Nuclear Agriculture, Mymensingh during 2005-2006. Six rhizobial strains were isolated from. Bangladesh. Characterizations of six strains were performed by two methodologies based on PCR amplification. These were PCR DNA fingerprinting of repetitive extragenomic palindromic (REP-PCR) sequences and Restriction Fragment Length Polymorphism (RFLP) analysis of PCR-amplified 16S-rRNA gene regions. Nodulation natures of strains were confirmed by PCR amplification of 800bp long nodC fragments. Nitrogen fixation, nodulation efficiency, acid and temperature tolerance was assays under laboratory condition. On the basis of Box profiles, five genotypes were identified among six strains. On the other hand PCR-RFLP analysis of intergenic genes coding for 16S r-RNA yielded intra-specific monomorphisms indicating that these six strains were closely related. RFLP profiles and Box profiles are not correlated because RFLP provides single locus analysis, whereas the latter assesses genetic variability in the whole genome. However, the REP-PCR based methods are much less time-consuming and are therefore more convenient. Nodulation genes found homogenous regarding fragment size of plasmid borne nodC gene. In lab experiment, Bangladeshi strains showed more nodule (23/plant) as well as affectivity (shoot dry matter weight 22mg/plant) and also showed survivable capacity in acidic conditions e.g. pH 4 & pH 10 and temperate condition such as 380c. Key words: Lentil, rhizobia, 16S r-RNA, REP-PCR, RFLP, nodulation, Bangladesh. 1Soil Microbiology, Bangladesh Institute of Nuclear Agriculture. 2 Department of Biology, University of York, United Kingdom. Corresponding author: Md: Harun-or Rashid E-mail: [email protected].

107

Red Cell Survival Study with Cr-51 Labeled Donor R.B.C Before and After Splenectomy of Thalassaemia Patients

Md.Ishraque Hossain Ansari a

Objective: Chromium-51 labeling of red cells is the most common technique at the present time for the measurement of red cell life span and is also used for evaluation of splenic sequestration. In this study R.B.C life span before and after splenectomy was determined to know whether slenectomy improves the life span of RBC. Methods: Ten Patients between ages 5-13 years were studied both before and after splenectomy. The patients received tagged donor blood of same group for determination of RBC survival. 10 ml of donor blood was drawn by heparinized syringe, kept in the conical flask, then 50 – 60 µCi 51Cr (Na2CrO4) was added. The mixed blood was incubated for 45 mins at 370C in a incubator by gentle shaking after each 10 mins interval. After 45 mins one ml (100mg) ascorbic acid was added to the blood and mixed by gentle shaking. After 3 mins 10 ml of the mixed blood was injected to the patient. One ml of mixed blood was kept for tagging fraction determination. Tagging fraction was determined by using formula, % TF = R.B.C cts. / (Plasma cts.+R.B.C cts.) × 100. Samples of 2 ml blood were collected in a heparinized syringe after 48 hrs of injection, then on 5th and 9th day. R.B.C of samples were separated and kept in refrigerator for counting. On the last day of collection, the R.B.C samples were counted in a well type gamma-counter. Days versus counts were plotted on a semi log graph paper and R.B.C life span was determined from T½ of the graph. Splenic sequestration using external probe counting was measured in 51Cr red cell survival study. Results: Highest labeling yield of ~90% was obtained with donor blood. R.B.C life span before splenectomy were shortened which improves to normal after splenectomy. The ratio spleen to liver 51Cr activity provides a rough measure of degree of hyperslenism. Conclusion: Highest labeling yield obtained using the donor blood. R.B.C life span improves to normal after splenectomy. Key words: Thalassaemia, R.B.C life span, splenectomy a Institute of Nuclear Medicine and Ultrasound, Bangladesh Atomic Energy Commission, Dhaka. Correspondence: Md.Ishraque Hossain Ansari, Senior Scientific Officer, Block-D, 7th-10th floor, BSM Medical

University (IPGMR) campus, Shahbag, Dhaka-1000, E-mail: [email protected] Telephone: 02-861957, Fax: 02-862561.

108

Effect of Levels of Soya Milk on the Storage Life of Dahi Prepared from Buffalo Milk

M. J. Uddin1, M. N. Sultana2, S. Basak2 and M. R. Hassan2

The present study was undertaken to measure the effect of using soya milk to evaluate the storage life of dahi at room temperature (31-32°C) and refrigeration temperature (5°C). In this experiment, four different types of dahi were prepared from buffalo milk with the addition of different levels of soya milk, the dahi samples were designated as ‘A’ (100% BM), ‘B’ (80% BM + 20% SM), 'C' (60% BM + 40% SM) and 'D' (40% BM + 60% SM). All the dahi samples were stored at room temperature (31-32°C) and refrigeration temperature (5°C).

Quality of dahi was also monitored by using physical and chemical tests. From the result of the physical study (smell and taste, body and consistency, color and texture), it was found that the overall score of A, B, C and D type of dahi samples statistically differ significantly kept at room temperature and at refrigeration temperature. In case of chemical tests, acidity value of dahi samples kept at room temperature and at refrigeration temperature differ significantly. It was observed that quality of dahi samples deteriorated rapidly at room temperature than refrigeration temperature. At room temperature all the samples were acceptable for human consumption from 48-72 hours and refrigerated samples were acceptable from 11-14 days after storage.

Finally, it is concluded that storage life was relatively longer when 20% soya milk was used to prepare dahi which would produce dahi nearly similar to the quality of buffalo milk. Key words: Dahi, storage life, buffalo milk and Soya milk. 1Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh 2Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Name of corresponding author: Md. Jamal Uddin, Biotechnology Division, BLRI, Savar, Dhaka-1341, Bangladesh. Email: [email protected]

109

Possible Pathway (Hypothesis) Of Food Chains In Natural Ecosystem through Which Human May Be Affected By Arsenic Poisoning

M. Mahfuzur Rahman 1 and M. Azizur Rahman 1

To investigate the possible pathways (other than drinking water) through which arsenic may deposit into human body, some ecological experiments were conducted. On the basis of the results found from the experiments, we proposed the present hypothesis as summarized below– Arsenic exists in soil environment as hydrous iron, manganese, and aluminium oxides, which may deposit through weathering, biomethylation, leaching or co-precipitation. Through the hand tube-well water, arsenic from soil solution and sorption on organo-mineral colloids is pulled out from aquifer. This arsenic contaminated water is used as drinking and cooking purposes. During 1970s, the UNICEF installed thousands of hand tube-wells throughout the country to provide safe drinking water. Thus, human may be exposed to arsenic poisoning directly from drinking water. This is the main pathway of human exposure to arsenic poisoning in Bangladesh and West Bengal, India. On the other hand, arsenic contaminated ground water is polled out through the shallow tube-well, which is mainly used in irrigation purpose in Bangladesh and West Bengal, India during dry season. Rainwater also becomes contaminated with arsenic from arsenical herbicides, pesticides and fertilizers, which are used as irrigation purpose too. This arsenic contaminated irrigation water is translocated to the aerial parts of aerobic rice plant and arsenic is loaded into rice straw, grain and husk. Arsenic is also deposited in surface soil and continual use of arsenic contaminated irrigation water, surface soil has been deposited with increasing arsenic concentration gradually. In Bangladesh, 32 ppm arsenic has been recorded in agricultural soil (20 ppm arsenic is the critical level for rice plant in soil). 0.5 ppm arsenic has been recoded in rice grain in our experiment while the safe level of arsenic concentration in rice grain is 1.0 ppm. But, it was estimated that a person drinking 4 liters of water per day containing 0.05 ppm arsenic (the acceptable limit of arsenic concentration in drinking water in Bangladesh) is expected to intake 0.20 ppm arsenic per day. On the other hand, the average daily consumption of rice by an adult person is about 400 to 600 gm raw rice per day. If rice grain contains 0.39 ppm arsenic (we found 0.39 ppm arsenic in grain when soil arsenic concentration was 30 ppm, the highest soil arsenic concentration found 32 ppm in agricultural soil of Bangladesh), the daily intake of arsenic into human body from rice grain would be about 0.156 to 0.234 ppm. This findings suggest that intake of arsenic into human body via rice grain is not less than that of throw water. Cattle are that primary consumer of food chain of ecosystem in nature. They eat rice straw and husk and also drink tube-well water and thus may become exposed to arsenic poisoning. The men are the secondary consumer as they eat beef and mutton. The majority (about 87%) of the population of Bangladesh is Muslim and they eat beef as a principle source of meat. Irrespective of religious identity, people have been drinking milk as a source of protein requirement. Thus, there is an ample possibility of arsenic deposition in cattle body and ultimately in human body through “Plant – animal – Man” food chain pathway. Volatile arsenic may contaminate the atmosphere when arsenic contaminated rice straw is burned. This volatile arsenic entire into human body with oxygen. Thus, men are not being exposed to arsenic poisoning from arsenic contaminated drinking water. There are some other major pathways through which men have been exposed to this poisonous chemical and these are that food chain of natural ecosystem. So, we should to give attention to the entrance of arsenic into human body through different food chain pathway other than drinking water. 1 Plant Ecology & Nature Conservation Lab. Botany Dept. Jahangirnagar University, Savar, Dhaka-1342, Bangladesh. E-mail: [email protected]

110

Genetic Divergences and Phylogenetic Relationships of Fejervarya Limnocharis Complex from Bangladesh and Some Other Asian Countries

Inferred From Mtdna Sequence and Allozyme Analyses

M. M. Islam1, N. Kurose1, M. M. R. Khan2, M. S. Alam1, and M. Sumida1 In the present study, allozymes and the nucleotide sequences of mitochondrial Cyt b, 12S and 16S rRNA genes were analyzed to elucidate the degree of genetic divergences and phylogenetic relationships among the F. limnocharis complex from Bangladesh and other Asian countries. Allozyme analysis was carried out at 28 loci encoding 20 enzymes and 2 blood proteins. When Nei’s genetic distances were calculated using PHYLIP, distinct genetic divergences were found among three size-types: mean genetic distances were 0.857 between small and middle types, 1.523 between large and middle types, and 1.521 between large and small types. Phylogenetic trees based on genetic distances showed that small type was most closely related to the Sri Lanka population, and large type formed cluster with several populations from Japan, Taiwan, Malaysia and Thailand, but middle type was segregated from any other groups. As for mtDNA gene sequences, after respective DNA extraction, PCR amplification and direct sequencing, we got the sequences and analyzed the data by using PAUP. We found a total of 21, 7 and 5 haplotypes for Cyt b, 12S and 16S rRNA genes respectively. Phylogenetic trees constructed by ML, NJ and MP methods showed that all Bangladeshi frogs were first divided into 2 groups, large type and the other types, and the latter were further subdivided into small and middle types. After comparison with other Asian Fejervarya species, we found that Bangladesh small type made a clade with F. syhadrensis from India and Sri Lanka, whereas the middle type was closely related to unnamed F. sp. from Myanmar, and the large type grouped with F. iskandari and had a close relation with F. orissaensis. Key words: Fejervarya; allozyme; mtDNA; nucleotide sequence, phylogenetic relationship; genetic divergence 1Institute for Amphibian Biology, Hiroshima University, Hiroshima, Japan 2Dept. of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh Name of corresponding Author: M. Sumida E-mail: [email protected]

111

Agrobacterium –Mediatged Genetic Transformation in Sugarcane of Bangladesh

M.A Hossaina, M.M Shaika, N. Islama, K.M Nasiruddina and M. A S Miaha

Agrobacterium–mediated genetic transformation system was developed for four sugarcane

varieties viz Isd 20, Isd 25, Isd 28 and Isd 31 regeneration of plantlets from transformed explants

were studied. The transformation efficiency in callus, organogenic pretreated callus and

meristgematic spindle leaf segments at three infection times such as 60, 90 and 120 minute using

five Agrobacterium strains was studied. The effects of acetosyringone during bacterial cultures

and in infection medium were also observed Varieties Isd 25 and Isd 28 showed the best

regeneration response from various transformed explants . GUS positives results were found

higher in varieties Isd 31 and Isd 20 whether acetosyringone was used in bacterial culture or

not. However, Isd 25 and Isd 28 showed the higher GUS positive results when acetosyringone

used only in infection medium than in bacterial culture medium along weigh infection medium,

PCR amplification of selected segments from GUS reporter gene was done using DNA isolated

from plantlets of varieties Isd 28 and Isd 31. In both varieties selected amplified DNA band was

found against two GUS primers set. Results indicate the Transformation of transgene in

sugarcane varieties of Bangladesh.

aBangladesh Sugarcane Reaearch Institute . Ishurdi-6620,Pabna ,Bangladesh.

Effects of Soluble Dietary Mixture on Fiber Enriched Cookie Processing

112

M. M. Shaik1, M. M. Rahman2, M. A. Haque3

Application of high-fiber ingredients in the cookie production for the enrichment of dietary properties can affect the dough rheology such as dough moisture content, dough viscosity as well as the quality of the products. The research was undertaken to study the effects of water soluble dietary fiber mixture in the production of fiber-enriched cookie and to compare two type cookies with and without fiber. Two type cookies were used as ‘control’. The first one was soft type cookie made of wheat flour (soft type), sugar, liquid glucose syrup, sucrose syrup, cornstarch, milk powder, vegetable fat, ammonium and sodium bicarbonate etc. The second one was hard type cookie made of wheat flour (hard type), sugar, milk powder, vegetable fat, butter, ammonium and sodium bicarbonate etc. Fiber-enriched cookies were prepared with the addition of 3% dietary fiber, where the wheat flour was substituted with carboxymethyl cellulose 2%, pectin 0.5%, and guar gum 0.5%. The dough moisture content of the fiber-enriched cookie was 17.98%. Whereas, the moisture contents of dough of control cookies were 13.91% and 15.51% respectively. Dough viscosity of fiber-enriched cookie was higher than the dough viscosity of the control cookies. After baking, improved volume and change of color of the product was observed. By the organoleptic evaluation of the baked products, improved crispiness and slightly hardness was observed in fiber-enriched cookie compared to the non-fiber cookies. Improved chewing properties and an unstable sticky mouthfeel were also observed in fiber-enriched cookie. Determination of moisture contents of cookies after baking indicated that, the moisture content of fiber-enriched cookie was less than the moisture content of soft type cookie, but higher than the hard type cookie. Analysis of the moisture absorption rate from the open air indicated that fiber-enriched cookie absorbed moisture from the air at an increased rate than the non-fiber cookies. Microbiological test indicated that, the fiber-enriched cookie had no microorganisms and was safe for human consumption. 1 Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia. 1 Biotechnology and Genetic Engineering Discipline, Khulna University. Khulna-9208, 3 Department of Diagnostic (Bacteriology Lab), King Institute of Preventive Medicine, India.

113

Impact of Hospital Liquid Waste Discharge in the Development of Antibiotic Resistance in the Environmental Bacteria.

K. T. Osman1, M. N.Anwar1, and M. A. Hossain2.

Emerging of antibiotic resistant bacteria becomes a serious growing problem in modern medicine. Misuse and/or extensive use and poor disposal system of antibiotics are the principal cause of resistance development in the bacteria. Un-treated hospital liquid and solid wastes discharge directly to sewerage systems largely contributing in the development of antibiotics resistance in bacteria and its distribution. The disposal systems of Chittagong Medical College Hospital (CMCH) wastes do not maintain proper hygiene and the liquid wastes directly discharge to the municipal sewerage systems. This raises the importance of investigating the involvement of CMCH liquid wastes discharge in the development and distribution of antibiotics resistance in the environmental bacteria. In this study, total 3 groups of samples (1, 2 and 3) connected with CMCH liquid wastes discharge and 2 control groups of samples (4 & 5) either indirectly- or non-connected with CMCH discharge were collected within two months period August – September 2006. The antibiotics used during the period to treat the patients in the hospital also recorded. The most frequently used antibiotic- ciprofloxacin in the CMCH wards and commonly detected environmental bacteria Escherichia coli were used as tools for the analysis of resistance development in the environmental microorganisms. Total bacterial count was very high at immediately connected sites to municipal sewerage system in both sample 1 (connected with CMCH waste) and sample 5 (non-connected to CMCH waste, control-2) and the counts decreased with increasing the distance of collecting sites from waste discharge site. Similarly, ciprofloxacin resistant bacterial count also found very high in the sample 1 and the number decreased with increasing the distance from the discharge site (sample 2 & 3), but the sample 5 showed very insignificant antibiotic resistant bacteria. In contrast, sample 4 (indirectly CMCH connected, control-1) counted resistant bacteria 263 folds more than sample 5 and this value was 38 folds less when compared with hospital waste site sample 1 when we counted the resistant colonies at 200ug/ml ciprofloxacin. 24 ciprofloxacin resistant E. coli colonies were isolated randomly from different samples to analyze the resistance pattern and plasmid profiles. All the isolates showed resistant to tetracycline, bacitracin, cephalexin, and penicillin G, but sensitive to imipenem and gentamycin. The isolates were resistant to very high concentrations of ciprofloxacin even >900 ìg/ml. The antibiotics resistant pattern observed in environmental E. coli isolates during this study clearly corroborate the antibiotics used by the practitioners randomly in CMCH. The plasmid profile of the 19 isolates revealed that all the isolates contained at least one high molecular size plasmid greater than 12 kbp except isolate number 6 and 16. Isolates number 7 and 8 contained two more plasmids of molecular size 5.6 kbp and 3.0 kbp respectively, and isolates 14, 18 and 19 also contained two plasmids of size 9.2 kbp and 4.0 kbp. Furthermore, isolates 11 and 12 also contained a plasmid of 9.2 kbp size in addition to the higher one. These findings conclude– i. CMCH waste contributes in the development and distribution of antibiotics resistance in the environmental bacteria through municipal sewerage system; ii. Observation of multi-drug resistant bacteria reflects the mis- or over- use of those antibiotics by the practitioners. Our findings suggest that hospital waste disposal system and use of antibiotics by the practitioners need to be monitored properly in order to avoid the emergence of antibiotics resistance in bacteria. Key words: Antibiotic resistance; CMCH, Hospital liquid waste; Ciprofloxacin; E. coli; Plasmids. 1 Department of Microbiology, University of Chittagong, Bangladesh. 2 Department of Microbiology, University of Dhaka.

114

Role of PKA Sub Cellular Anchoring in the Glucagons-Induced Camp Stimulation of Insulin Secreting Pancreatic Cells

M Omar Faruque1, Dung Lenguyen2, Anne-Dominique Lajoix2, Eric Vives3, Pierre Petit2,

Dominique Bataille2, Liaquat Ali1, El Habib Hani2

Background: Cyclic AMP is generated following receptor activation by many hormones and neurotransmitters which lead to stimulate PKA activity and subsequent phosphorylation of a variety of cellular substrates. Specificity this signaling pathway is influenced by factors that determines spatio-temporal activation. Subcellular localization of PKA occurs through association with A Kinase Anchor Proteins (AKAP). Using bioinformatics and protein array techniques a novel peptide, named AKAPis, has been proposed which competitively dis-anchors PKA RIIα-AKAPs interactions in vitro. Objective: To evaluate the importance of PKA-AKAP interactions in insulin secretion and stimulation of PKA target proteins of CREB and MAP kinase in pancreatic INS1 cell line.

Methods: AKAP-IS has been synthesized and linked with TAT peptide to have a construction TAT-AKAPis. This original peptide has been used to observe on the glucagon-stimulated cAMP-PKA signals in pancreatic β-cell (INS1). INS1 cells were grown up to 70% confluence in RPMI containing 10mM glucose, 10% serum, 50µM β-mercapto ethanol, 100U/ml penicillin, 100 µg/ml streptomycin. In the day of experiments cells were starved in hepes-balanced KRB buffer for 2h, TAT-AKAPis was added in the middle of the starvation period. Insulin secretion in response to glucose and glucagon was measured using HTRF kits. PKA RIIα-AKAPs interaction was determined using immunoprecipitation followed immunoblot techniques. Glucagon-stimulated CREB and MAP kinase signal was determined using Western blot.

Results: TAT-AKAPis dose dependently disanchored PKA RIIα-AKAP interactions. At 20µM concentration of the peptide RIIα-AKAP95 complexes has been reduced by 55%. Immunoflourescence study also confirms this disruption of RIIα-AKAP complexes through delocalization of PKA-RIIα with TAT-AKAPis. This disanchoring effect further affects significantly on insulin secretion and at 20µM of TAT-AKAPis, glucagon potentiated insulin secretion has been decreased by 80% in INS1 cell line. Phosphorylation of PKA target proteins like CREB and MAP kinase also significantly decreased in glucagon-induced INS1 cells when TAT-AKAPis was used.

Conclusions: a) Subcellular localization of PKA through interaction with AKAPs is an additional mechanism for insulin secretion in glucagon-potentiated pancreatic INS1 cell line, b) PKA-AKAPs interaction is an important factor for the stimulation of PKA target proteins like CREB and MAP kinase. 1Dept of Biochemistry & Cell Biology, Research Division, BIRDEM, Dhaka 1000, Bangladesh. 2CNRS UMR-5160, Centre de Pharmacologie et Biotechnologie pour la Santé, Faculté de Pharmacie, Montpellier Cedex 5, France. 3INSERM EMI0227 Centre de Recherche en Cancerologie, Montpellier Cedex 05, France.

115

Present status of Biotechnology in Animal Production in Bangladesh

M.O.Faruque1, S.A. Aziz2, M.A. Alim3

Biotechnology provides new opportunity to increase animal production/animal improvement. In animal production, biotechnology is applied in the field of Animal Genetics/Animal Breeding, Animal Reproduction, Rumen metabolism and fermentation of dairy products in dairy industry. The widespread use of biotechnology in Bangladesh is Artificial Insemination in Animal Reproduction and production of yogurt in dairy industry. So there is ample scope to adopt the other biotechnologies for increasing animal production/animal improvement. Attempts are being made in different institutes involved in livestock research and education to adopt such technologies. Animal Genotyping for breed identification of livestock species/ breed are in progress in the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh. Genotyping of buffaloes and goats have been done and research is in progress for genotyping of cattle. Experiment has been set for mapping genes of quantitative traits of goat in Bangladesh by the same department. In vitro embryo culture is also in progress. Key words: Bangladesh, Animal Production, biotechnology 1Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh 2Department of Animal Breeding and Genetics, Sylhet Agricultural University, Sylhet, Bangladesh 3Breed upgradation through progeny testing project, Department of Livestock Services, Savar, Dhaka Corresponding author: Dr. Md. Omar Faruque E mail: [email protected]

116

Commercial Enzyme Production by Recombinant DNA Technology: A Conceptual Works

M.R. Anower 1

Until the advent of genetic engineering, enzyme producers were limited in their ability to produce innovative products for the marketplace. They were constrained to isolate enzymes from organisms approved for the industrial use. The desired characteristics could be enhanced only using classical in lit agenesis techniques. When these methods failed, no alternatives were available. Commercialization depended on incremental yield improvements gained by continuous programs of strain development. The ability to use recombinant DNA (rDNA) techniques has removed many of these barriers. Enzyme producers recognized early the potential for commercialization new products using genetic manipulation. They worked with a wide variety of single-celled organisms that were simpler and thus, easier to understand then the higher orders of plants and animals. The organisms already were well characterized for growth and expression rates. Short life cycles allowed rapid testing. These systems were ideal for genetic manipulation using rDNA techniques. Genetic engineering, combined with an understanding of biocatalysts to predict alterations for enzyme improvements, is revolutionizing the production and use of enzymes in the marketplace. Offering a recombinant produced product represents the culmination of a long and complex effort on the part of a multitude of disciplines: molecular and microbiology, X-ray crystallography, enzymology, protein and organic chemistry, biochemistry, fermentation and formulation engineering, assay chemistry and technical service/applications, marketing, sales. Because of the variety of disciplines required, a critical mass is needed to innovate products successfully and them to market. The continued proliferation of novel enzyme products requires development of core technologies so complex and expensive that they can be justified only rDNA technology must consider regulatory issues, ownership protection, and consumer acceptance. Key words: rDNA, enzyme, gene, RNA, protein 1 Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh

117

Collection, In Vitro Maturation, Fertilization and Subsequent Development of Goat Oocytes in View of

In Vitro Production (IVP) of Embryos

M.R. Islam1, S. Afroz2 and M.A.M.Y. Khandoker2

An alternative to conventional superovulation procedure is in vitro production (IVP) of embryos from slaughterhouse ovaries might be considered as a low cost and sustainable technology in Bangladesh. The study was undertaken to establish the suitable culture condition for in vitro maturation, fertilization and subsequent development of goat embryos at Bangladesh Agricultural University (BAU), from January 2004 to December 2005. Goat ovaries were categorized as CL- present group & CL- absent group. Among 146 ovaries CL found in 25 ovaries and remaining 121 ovaries having no CL. Significantly (p<0.05) higher number of normal COCs (1.12 ± 0.07) were found in CL- absent group with an increase of length (1.14 ± 0.02), total number of follicle (4.45 ± 0.19), number of follicle aspirated (2.55 ± 0.10) and total number of COCs (1.87 ± 0.09), but decrease in weight (0.64 ± 0.01), width (0.75 ± 0.01) and abnormal COCs (0.74 ± 0.07) per ovary than CL- present group of ovaries. The collected COCs were then cultured for 22 hours in TCM-199 medium supplemented with 5% fetal calf serum (FCS) in a condition of 5% CO2 in air at 38.5°C for maturation. The higher maturation rate was observed in CL- absent group (75.00 ± 3.62) than CL- present group (70.29 ± 4.20) of ovaries. Matured COCs were cultured for five hours with fresh buck semen in Brackett and Oliphant (BO) medium. In progress IVC was practiced in TCM-199 medium supplemented with 5% fetal calf serum (FCS) in the same condition for 6 to 7 days. The rate of development to blastocyst was found higher in CL- absent group (27.60 ± 4.76) compared to CL- present group (21.50 ± 4.64) of ovaries. CL-absent group ovaries are seems to be suitable for the IVP of goat embryos in Bangladesh. Key words: Goat ovary, IVM, IVF, IVC, IVP

1Animal Division, National Institute of Biotechnology (NIB), AERE, EPZ, Savar, Dhaka-1349, Bangladesh 2Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, BAU, Mymensingh-2202, Bangladesh Name of corresponding author: Md. Rashedul Islam; Email: [email protected]

118

Characterization of Different Strains of Common Carp (Cyprinus Carpio L.)(Cyprinidae, Cypriniformes) in

Bangladesh Using Microsatellite DNA Markers

Md. Rashedul Kabir Mondol1, Md. Shahidul Islam2 and Md. Samsul Alam3 Characterization of different strains of common carp (Cyprinus carpio L.) using molecular markers is essential for the management of this fish in respect to the evaluation of the potential genetic effects induced by hatchery operations and the genetic improvement of carp varieties. Five microsatellite loci (MFW1, MFW2, MFW11, MFW15 and MFW20) were analyzed for the molecular characterization of four common carp strains, i.e. scaled carp, mirror carp, red carp and koi carp. We observed differences in heterozygosities and the average numbers of alleles but not in polymorphic loci (P95) among the strains. Koi carp displayed the highest level of variability in terms of heterozygosity. The Nm values and the FST values indicated a low level of gene flow and high level of differentiation among the strains. The highest genetic distance was observed between the scaled carp and the koi carp whilst the lowest genetic distance was found between the red- and koi carp. The unweighted pair group method with averages (UPGMA) dendrogram resulted in two clusters, one containing only the scaled carp and the other the remaining three varieties. Microsatellite markers have been found to be effective tools for characterization of different strains of common carp. Key words: characterization, Cyprinus carpio, microsatellite marker. 1Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh. 2Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh. 3Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh. Name of corresponding author: Md. Rashedul Kabir Mondol Email: [email protected]

119

Molecular Characterization of Groundnut (Arachis hypogaea L.) Varieties Using SSR Markers for Variety Protection

Md. Rezwan Molla1*, Mohammad Nazrul Islam2, Md. Shefatur Rahman1, Md. Samsul Alam2 and Lutfur Rahman1

Cultivated peanut or groundnut (Arachis hypogaea L.) is an important crop for oil and protein source in Bangladesh. A number of varieties have so far been released or registered for cultivation in the country. These have been distinguished only through morphological traits. Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be an excellent tool for the identification of the plant varieties and determining genetic relationship between the varieties of a crop species. A set of three SSR markers namely, PM36, PM50 and PM238 were used to identify ten cultivated groundnut varieties (Dhaka-1, Bashanti, Tridana, Zhinga badam, BARI badam 5,6,7; BINA Cheenabadam 1,2,3) available in Bangladesh. All the cultivars were successfully discriminated by these three SSR primers. The primer PM50 alone was able to distinguish four varieties (Dhaka-1, Bashanti, Tridana and Zhinga Badam). Six variety- specific alleles were identified, these are, PM36/227, PM50/110, PM50/116, PM50/118, PM50/137, PM238/200. The three primers produced a total of 13 alleles with size ranging from 109bp to 241bp. The PIC (Polymorphism Information Content) value for the primer PM36, PM50 and PM238 was found 0.81, 0.76 and 0.82 respectively. This approach will be useful for developing a set of limited number of SSR loci for the identification of commercially important groundnut varieties for purpose of obtaining plant variety protection (PVP) in Bangladesh. Key Words: Groundnut, Microsatellite marker, Variety Identification. 1Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymenisingh; 2Department of Fisheries Biology and Genetics, BAU, Mymensingh; *Corresponding Author: ([email protected]) Studied under the funding support of the DANIDA for Seed Industry Development of the Ministry of Agriculture.

120

Hori Dhan: Genetic Variation of BR11?

M. S. Rahman1, R. Begum2 and Z. I. Seraj2 Land races are derived with the knowledge, experience and continuous efforts of our farmers, despite the fact that they do not have any formal scientific training. One such cultivar named Hori dhan was developed by Horipada Kapali, an agrarian farmer of Asannagar, Jhinedah district. This variety was selected from a field, where BR11 and other HYVs were normally cultivated. The publicity regarding this discovery that was created by the print and electronic media raised many questions in the minds of rice growers and scientists. Hori dhan’s similarity to the most popular Bangladeshi rice variety BR11 (Mukta) resulted in a debate on its origin. This work describes a comparative evaluation of the morphogenetic variations between Hori dhan and BR11. The study was conducted at the Bangladesh Rice Research Institute (BRRI) and Department of Biochemistry and Molecular Biology, University of Dhaka during June 2006-February 2007. Thirty days old seedlings of both varieties were transplanted in well prepared puddle field of 3x3m plot for comparisons of morphological features, and molecular characterization were done using the leaf samples of the seedlings. Randomized Completely Block Design with 3 replications was used to conduct the field trial. Fertilizers and other cultural practices were followed by the recommendation of BRRI for growing the crop up to maturity. Ten randomly selected hills were labeled to characterize the morphological features and the rest were used to measure the yield. DNeasy plant mini kits (QIAGEN) were used to extract genomic DNA and 70 microsatellite markers distributed over the 12 rice chromosomes were used for characterization. Considerable variations were found both in morphology and at molecular level. Hori dhan has a taller stature and is around 9.0 cm taller than BR11. Flag leaf, 2nd leaf and ligule were found to be 2.5cm, 2.4 cm and 2.0 mm longer in Hori dhan compared to BR11. Flag leaf angle of Hori dhan is erect while that of BR11 is intermediate. Hori dhan was found to have longer panicles, more filled grains, and larger grain size (26.20cm, 779.00/hill and 2.49g/100 grain, respectively). This contributed to slightly higher yield (4.94 t/ha) in Hori dhan compared to BR11 (4.39 t/ha). Interestingly Hori dhan can be characterized by the presence of awn in its 1st spikelet in the panicle. Hori dhan also has longer growth duration of around 9 days compared to BR11. Using molecular markers, variations were found in several loci of chromosome number 2, 8, 11 and 12, but not in other chromosomes. This confirms that Hori dhan is genotypically different from BR11. Due to its tall stature it may however be under risk of lodging. Key words: Hori dhan; BR11; Genetic Variation; BRRI 1Plant Physiology Division, BRRI, Gazipur-1701, Bangladesh 2Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh Name of corresponding author: Md. Sazzadur Rahman Email: [email protected] Cell Phone: 01722210429

121

Deduction of Coding Sequence of a Jute Gene Using 5’ RACE and Its Bioinformatics Analysis

Md. Shakhinur Islam 1, Aleya Awal1, Nazlee Sharmin2, Maksud1, Haseena Khan1*

Jute is a dicot shrub and is second only to cotton in amount produced and variety of uses. In recent years, researchers are getting interested in improving agronomic jute traits through molecular approaches, which is however slowed down by the fact that the whole genome sequence of jute is not yet available. Moreover, most of the small number of jute sequences deposited in GenBank are uncharacterized. In this context, we sought to carry out bioinformatics analysis of a new jute gene, obtained by 5′ RACE, with a view elucidate its structure and function. Both the local and global alignment result confirmed that the gene might be a low density lipoprotein like protein (LDLP). To understand the function of LDLP in plant cell, we carried out primary and secondary structure as well as hydrophobicity analysis. The strong hydrophilic nature and extended coiled-coil region confirmed that the gene might code for a protein domain, involved in protein or lipid binding, indicating binding and storage of specific kinds of lipoprotein particles as one possible function of LDLP in jute. Key words: 5` RACE (Rapid amplification of cDNA ends), Low density lipoprotein like protein (LDLP).

1. Plant Molecular Biology Laboratory, Dept. of Biochemistry and Molecular Biology, University of Dhaka, 2. Department of Genetic Engineering and Biotechnology, University of Dhaka. * Corresponding Author : ([email protected])

122

DNA Fingerprinting of Rice (Oryza sativa L.) Cultivars Using Microsatellite Markers

Md. Shefatur Rahman 1*, Md. Rezwan Molla 1 and Lutfur Rahman 1

Microsatellite combines several features of an ultimate molecular marker and they are used increasingly in various plant genetic studies and applications. In this work, we report on the utilization of a small set of three previously developed rice microsatellite markers for the identification and discrimination of 17 HYVs and 17 local rice cultivars including two wild rice. All analyzed microsatellite markers were found to be polymorphic with an average number of 6.33 alleles per locus. These three markers were able to identify 15 local rice cultivars and 11 HYVs. A total of three specific alleles, RM-11/147, RM-151/289 and RM-153/178 were identified for BR-11, Badshabhog and BR-19 cultivars respectively. The average Polymorphism information content (PIC) value for local rice cultivars was 0.70 which is higher than that of the HYVs, suggested that local cultivars are more diverse that the modern high yielding rice varieties. DNA fingerprints of rice cultivars by means of microsatellites provided meaningful data, which can be extended by additional microsatellite markers. The data obtained can be used for the protection of Plant Genetic Resources. Key words: Rice, microsatellite markers, variety identification 1 Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymenisingh; *Corresponding Author ([email protected]) Studied under the funding support of the DANIDA for Seed Industry Development of the Ministry of Agriculture.

123

Endophytic Fungi are the Sources of Novel Pharmaceuticals

M. Shoeb1, M. Mosihuzzaman1 and N. Nahar1 Natural Products, derived from microorganisms and plants, have been used for the treatment of various diseases for thousands of years. Terrestrial plants have been utilized as medicines in Egypt, Africa, South America, Indian subcontinent and Greece from ancient time and an impressive number of modern drugs have been developed from them. The World Health Organization estimates that approximately 80% of the world’s inhabitants rely on traditional medicine for their primary health care. Thirty-nine percent of the 520 new drugs approved between 1983 and 1994, were natural products and the proportion of antibacterials and anticancer drugs derived from natural products, was more than 60%. Despite the advent of modern drugs, various types of new diseases have emerged recently, and microbes are becoming resistant to current drugs. Moreover, many synthetic agricultural agents have been targeted for elimination from the market because of safety and environmental problems. As a result, there is a general requirements for new antibiotics, chemotherapeutic agents and agrochemicals that are highly effective, possess low toxicity, and will have a minor environmental impact, respectively. Novel natural products and the organisms can offer opportunities for innovation in new drug and agrochemical discovery. The word endophyte means “in the plant” and refers to all microorganisms that live in the intercellular spaces of stems, petioles, roots and leaves of plants causing no apparent symptoms of disease. Some species of endophytic fungi have been identified as sources of anticancer, antidiabetic, insecticidal and immunosuppressive compounds. We have launched research work on endophytic fungi with Terminila Chebula Retz (Haritoki). Twenty one endophytic fungi were isolated from the leaf, stem and root of the plant T. chebula and one of the strain 1R7, identified as Pencillium thiomii, yielded one new compound. Later, the project expanded with the work of Ocimum basilicum, Momordica charantia, Lucus indica and Vinca rosea. Several fungi and secondary metabolites have been isolated from them and some of them have shown antibacterial activity. In this present we will report the natural products from endophytic fungi, chromatographic techniques for isolation and spectroscopic tools for identification of compounds. 1Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh Name of the corresponding author: Dr M. Shoeb E-mail: [email protected], Fax: 8615583

124

Genes Transcribed by Rhodococcus equi Inside Macrophages and Under Selected Environmental Conditions

M.T. Rahman1, V.M. Nicholson2, and J.F. Prescott2

Rhodococcus equi is a facultative intracellular respiratory pathogen of foals that persists and multiplies within macrophages. Virulence is associated with 80-90 kb plasmids, which include a pathogenicity island (PI) containing the vap (virulence-associatedprotein) gene family. However, the role of chromosomal genes in virulence is not known. Preliminary analysis of a partial (25-30% coverage) genome sequence of R. equi described in this thesis revealed homologues of Mycobacterium tuberculosis putative virulence genes. A mini-DNA microarray was developed with 43 selected putative R. equi chromosomal virulence genes and eight PI genes to study their transcription during growth of R. equi in vitro (37° C, pH 5.0), under oxidative stress conditions (50 mM H2O2, 30 minutes) and inside equine peripheral blood macrophages. choD, clpB, fadD13, fbpB, groEL, hemE, mbtF, moxR, and sigK were chromosomal genes significantly transcribed in R. equi inside macrophages. The expression pattern of R. equi chromosomal genes transcribed significantly inside macrophages differed from those transcribed under in vitro conditions.

Key words: Rhodococcus equi, Genome sequencing, Gene expression

1Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. 2 Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Name of corresponding author: Md.Tanvir Rahman Email: [email protected] Fax: +88-091-55810

125

Association of INS VNTR Polymorphism in Young Onset Diabetes Subjects of Bangladesh

Z. Hassan1, M. I. Hawa2, M. O. Faruque3, K. B. Biswas1, R. Islam1, K. Azad4,

A. K. Azad Khan5, L. Ali3, R. D. G. Leslie2, G. A. Hitman2;

Aims: A remarkable heterogeneity in clinical and biochemical presentation creates a special problem in the characterization and classification of young diabetic patients in Bangladesh. In an attempt to characterize these patients we have studied INS VNTR T/A polymorphism in these patients in relation to their insulin secretory capacity and autoantibody status.

Methods: Young (under 30 years) nonketotic diabetes mellitus (YDM, n=372) subjects and 332 age-matched healthy controls were studied. INS VNTR T/A polymorphism was analyzed by PCR-RFLP using endonuclease Hph1 and serum C-peptide was measured by ELISA. Insulin secretory capacity (HOMAB) was assessed by Homeostatic Model Assessment. GAD antibody was determined by radioimmunoprecipitation.

Results: Mean (±SD) BMI in the controls was 19.7±3.2 and YDM subjects 18.3±4.9 (p<0.001). INS VNTR T/A genotype frequencies did not show any significant difference between Controls and YDM (homozygous wild 0.753 vs 0.712; heterozygous variant 0.229 vs 0.259 and homozygous variant 0.018 vs 0.030). HOMAB (%, median-range), in YDM subjects [1.02 (0.11-2.11)] was significantly lower compared to the controls [1.94 (1.57-2.34), (p<0.001)]. HOMAB in the controls with wild TT genotype [1.94 (1.57-2.34)] did not show any difference with variant (TA and AA) genotype [1.96 (1.68-2.32)], but in YDM group subjects with wild TT genotype [0.96 (0.11-2.17)] had significantly lower value compared to those with variant genotype [0.1.13 (0.15-1.89), (p=0.019)]. GAD antibody was positive in 3.2% of the controls and 22.6% of YDM subjects. GADpositive YDM subjects had significantly lower HOMAB [0.67 (0.11-0.84)] compared to the negative cases [1.12 (0.11-2.17), (p<0.001)]. The proportion of wild genotype was significantly higher in GAD positive cases (83%) compared to negative cases (69%, p<0.018).

Conclusions: The association of INS VNTR wild TT genotype with GAD antibody positivity and lower insulin secretion suggest that young diabetic patients in Bangladesh include a subgroup of typical type 1 diabetic patients with atypical clinical presentation and residual insulin secretory capacity. 1Physiology and Molecular Biology, BIRDEM, Dhaka, Bangladesh,

2Centre for Diabetes and Molecular Medicine, Bart’s and The London Queen Mary’s School of Medicine and Dentistry, London, United Kingdom, 3Biochemistry and Cell Biology, BIRDEM, Dhaka, Bangladesh, 4Dept of Pediatrics, BIRDEM, Dhaka, Bangladesh, 5Dept of Gastroenterology, BIRDEM, Dhaka, Bangladesh.

126

Biological Screening of Spice Materials for Bioactivity against Tribolium Castaneum (Hbst.) Adults

K Farhana 1, H Islam 1, E H Emran 1 and N Islam1*

Extracts of coriander, ajowain and fenugreek seeds were collected in chloroform and screened for the presence of bioactive principles through residual film assay and repellent activity test and found promising for the control of the stored grain pest, Tribolium castaneum (Hbst.) adults. The LD50 values for the chloroform extracts of coriander were 316.173 and 243.5895 µg/cm2 for 24- and 48 h of exposure, for ajowain were 271.4573 and 232.7095 µg/cm2 for 24- and 48 h of exposure and for fenugreek seeds were 159.0106 and 4.194236 µg/cm2 for 24- and 48 h of exposure. The toxicity could be arranged in the order: fenugreek> ajowain>coriander seed, while the repellent activity could be arranged in the order: fenugreek seeds> coriander> ajowain. The experimental spices were found promising for the control of the red flour beetle.

Key words: Spices, biological activity, Tribolium castaneum

1 Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

* Corresponding author; e-mail:[email protected]

127

An Easy and Efficient DNA Isolation Protocol for Fingerprinting Using RAPD Markers of Sugarcane

M.A Hossaina, N. Islama, R.M.S Shahnawaza, M.M Shaika and M.A S Miaha

. An easy and efficient method was adopted for isolation of quality DNA from meristem cylinder in four sugarcane varieties such as Isd 16, Isd 29, CP 70-1133 and Poj 2878 . The quality and quantity of DNA were determined by visual estimation of agarose gel electrophoreses and by UV spectrophotometer. The yield of DNA was depended on varieties of sugarcane. The highest amount of DNA was recovered from the variety CP-70-1133 and the lowest amount was obtained from the variety Poj 2878. The amount of recovered DNA was enough for Polymerase Chain Reaction (PCR) amplification and marker studies such as random amplified polymorphic DNA (RAPD). DNA fingerprinting of four sugarcane varieties was performed using RAPD markers. Bands obtained from fingerprinting showed 58.72% polymorphism. Dendrogram based on linkage distance using Unweighted Pair Group Method of Arithmetic Means (UPGMA) indicated segregation of four varieties of sugarcane into two main clusters. Varieties Isd 29, CP70-1133 and Poj 2878 grouped in cluster 1 followed by sub-clusters again however, variety Isd 16 alone was in cluster 2. DNA isolated using the adopted method was sufficient to perform RAPD a potentially simple, rapid, and reliable method to evaluate genetic variation among sugarcane varieties.

a Bangladesh Sugarcane Research Institute. Ishurdi –6620, Pabna Bangladesh

128

Overcoming Breeding Barriers of Interspecific Hybridization in Buckwheat: an Ultrastructural Analysis and Future Prospects

Nilufar Yasmin Shaikh and Taiji Adachi

The common buckwheat (Fagopyrum esculentum), a very popular human food source of the world, has seeds with high nutritional and health benefit attributes. The crop grows in marginal lands with poor soil conditions where a major crop is not sustainable. It is being sparsely grown in the northwestern part and has a good potential for cultivation in the vast underutilized and fallow lands of the Hill Districts of Bangladesh. There is a great demand for buckwheat in Europe, Japan, Korea etc. and hence the country has immense potential to export the commodity at a much higher price than rice and wheat. The main constraint of buckwheat production is its low seed set and instability resulting from self-incompatibility and failure of fertilization. Improvement of seed yield was attempted through interspecific hybridization among four Fagopyrum species but overcoming barriers of interspecific hybridization became a pre-requisite for obtaining a hybrid. Attempts were made to identify the breeding barriers of interspecific hybridization between F. cymosum and F. esculentum as well as between F. tataricum and F. esculentum through investigation of early embryo developmental stages by light microscopy and transmission electron microscopy (TEM). The nature of pre- and post-fertilization barriers in the hybrid embryo was examined at the ultrastructural level. The ovules were excised at 1-5 days after pollination (DAP) and fixed for TEM. The interspecific hybrid embryos showed numerous abnormal ultrastructural pre- and post-fertilization phenomena leading to abortion of embryo. The main abnormal phenomena included: failure of fertilization, no zygotic development, delay in pro-embryo development and degeneration of embryo and endosperm. It was thus clear that the ultrastructural abnormalities occurred at very early stages of development leading to the abortion of the embryo. Comperatively fewer abnormalities occurred in the hybrid embryos of the cross between F. cymosum and F. esculentum than that of F. tataricum and F. esculentum. This indicated that the former cross combination may be considered as a breeding material in buckwheat hybridization. The hybrid zygotic embryo showed retardation in cell divisions resulting in no zygotic development. The deficiency and degeneration of endosperm that were observed at 2 to 3 DAP may lead to the degradation of the hybrid embryo. Therefore, rescue of the hybrid embryos at this critical stage by ovule culture may overcome one of the main post fertilization barriers.

129

Emergence of Optochin-resistant Streptococcus pneumoniae: Implications for Diagnosis and Management of Pneumococcal Diseases

M. Rahman1, H. Rashid1, N. Nasrin2, K. Zaman1, N. Nahar1, and A.K.A. Chowdhury2

The optochin susceptibility test remains the primary and, in some cases, the only method in clinical microbiology laboratories to differentiate S. pneumoniae from �hemolytic viridans streptococci. However, emergence of optochin resistance in S. pneumoniae results in misidentification of pneumococci, lowering their isolation rate that jeopardizes the diagnosis, treatment and prevention of pneumococcal diseases. The study was conducted in the ARI lab (LSD) of ICDDR,B. Fifteen Hundred nasopharyngeal swabs of mothers and infants enrolled in the study were subjected to the tests used to identify S. pneumoniae such as colony morphology, gram staining, optochin susceptibility and bile solubility tests and finally confirmed by lytA (autolysin) PCR. Antimicrobial susceptibility test and MIC value detection were also performed to see the emergence of drug-resistance pattern among the strains. In total, 111 optochin-resistant �hemolytic streptococci strains were detected. When subjected to bile solubility test, 37 (33.3%) optochin-resistant, bile soluble S. pneumoniae were obtained, as other �hemolytic-streptococci were not bile soluble. An S. pneumoniae-specific lytA gene PCR was positive in all 37 isolates confirming their identification. Thus, the optochin susceptibility test failed to differentiate S. pneumoniae from other streptococcal species, showing its decreasing sensitivity and specificity. Optochin-resistant S. pneumoniae had significant co-resistance to other antimicrobial agents (64.86% were resistant to penicillin, 78.37% to co-trimoxazole, 78.37% to ciprofloxacin, 45.95% to tetracycline and 27.03% to azithromycin/erythromycin) and multi-drug-resistance (resistant to ≥3 drugs, 64.86%). Emergence of optochin-resistance in S. pneumoniae threatens the diagnosis, therapy and prevention of pneumococcal diseases because of failure to detect S. pneumoniae. For correct identification and, consequently, for correct treatment, �hemolytic-streptococci with a typical colony morphology of S. pneumoniae but optochin resistant should be checked by bile solubility test or PCR for S. pneumoniae. Alternatively the bile solubility test should be routinely used for identifying S. pneumoniae. Keywords: hemolytic-streptococci 1ICDDR,B, GPO Box 128, Dhaka-1000, Bangladesh 2Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Ramna, Dhaka-1000, Bangladesh Name of the corresponding author: Mahbubur Rahman E mail: [email protected]

130

Characterization of Insulin Secretory Defect in Bangladeshi Type 2 Diabetic Subjects

R. Zinnat1, R. Karim2, S. Islam2, L. Ali1

Background and Aims: It still remains controversial whether insulin deficiency or insulin resistance is the earliest abnormalities in the natural history of the disease. Considerable heterogeneity seems to exist regarding these abnormalities among various populations. Previous studies suggest that insulin secretory dysfunction is the relatively more prominent defect in Bangladeshi nonobese type 2 diabetic population. The characterization of the defect may provide valuable tools for further understanding of the role of insulin secretory defect in the pathogenesis of type 2 diabetes. The aim of the present study was to investigate the nature of insulin secretory defect in type 2 diabetic subjects in various phases of secretion. Materials and Methods: Forty-four type 2 diabetic subjects, along with 30 Age- and BMI-matched control subjects, were studied. The diabetic subjects were never treated with insulin. Insulin was measured by chemiluminiscent ELISA. Insulin secretory capacity (%HOMA B) and insulin sensitivity (%HOMA S) were calculated by homeostasis model assessment (HOMA) using HOMA software. First and second phases of insulin secretion were calculated by the following equations: 1st phaseest =1283+1.829.Ins30-138.7.Glu30+3.772.Ins0; 2nd phaseest=287+0.4164.Ins30-26.07.Glu30

+0.9226.Ins0 Results: The mean age (years, M±SD) of the patients was 44±7 and that of controls 41±7. BMI (kg/m2, M±SD) was 25.46±3.26 in patients vs 25.31±3.61 in controls. Fasting serum insulin [Median (range); 53.96 (6.67-162.58) vs 74.55 (6.25-216.89), p=0.0001]; and 30 min after glucose insulin [Median (range); 132.44 (17.78-422.19) vs 544.59 (33.27-1168.64), p=0.013] levels were significantly lower in diabetic as compared to control subjects. The B-cell function was significantly lower in diabetic subjects as compared to the controls [%HOMA B, Median (range); 53.90 (10.40-162.90) vs 163.60 (48.40-381.70), p=0.0001], but no significant difference was observed in case of insulin sensitivity [%HOMA S, Median (range); 79.10 (26.40-226.90) vs 65.20 (21.90-259.90), p=0.088]. The 1st and 2nd phases of insulin secretion were also significantly lower in diabetic subjects compared to control [Phase I: Median (range); 208.49 (-1412-1230) vs 1655.56 (-109-2831), p=0.0001, Phase II: Median (range); 101.23 (-217-341) vs 418.56 (51-679), p=0.0001]. A significant positive correlation was found between BMI and Phase I (r = 0.315; p = 0.037) and Phase II (r = 0.320; p = 0.034) of insulin secretion in case of diabetic subjects. No such correlation was observed in case of the control subjects. A significant negative correlation was also observed between insulin sensitivity and BMI (r = - 0.439; p = 0.003) in the diabetic subjects. Conclusion: Nonobese type 2 diabetic patients in Bangladesh have insulin secretory dysfunction and they do not seem to have insulin resistance as a primary pathophysiologic characteristics. The B-cell secretory dysfunction in type 2 diabetics involves both fasting and poststimulatory states; poststimulatory defect, however, is much prominent in comparison to the fasting state. Both 1st and 2nd phases of insulin secretion are affected in type 2 diabetes, but the deficiency in 1st phase secretion seems to be predominant in these subjects.

1Dept of Biochemistry & Cell Biology, BIRDEM, Dhaka, Bangladesh, 2Dept of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.

131

Fine Mapping and Progeny Testing in Rice to Identify Flanking Markers for Introgression of Salt Tolerance

3Rejbana Alam, 3Suhaila Rahman, 3Habibul Bari Sozib, 3Aleya Ferdousi, 1Sazzadur

Rahman. 2Rafiqul Islam and 3Zeba I. Seraj

DNA markers linked to salinity tolerance traits can make breeding programs more efficient. A major quantitative trait locus for salinity tolerance traits was reported to be present in rice chromosome 1. Fine mapping using near isogenic lines (NILs) between tolerant donor Pokkali and sensitive recipient IR29 contributed by the International Rice Research Institute has limited the linked region between 11.2 and 12.7 million base pairs or mbp (5 cM). Subsets of the mapping populations show linkage at 11.2-11.45, 12.0-12.3 and 12.6-12.7 mbp. Marker-trait linkage in breeding populations where the major salt tolerance donor is Pokkali, are being used to confirm position of linkage. Breeding populations being raised at BRRI, will be tested using identified markers in a marker-assisted backcrossing approach to introgress salinity tolerance into mega farmer-popular varieties like BR11 and BR29. 1Plant Physiology Division, Bangladesh Rice Research Institute, Gazipur 2Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur 3Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000

132

Construction of Vectors with Different Genes and Promoters to Produce Salt Tolerant Rice by Transformation

Richard Malo1, Rakibul Islam1, Mahzabin Amin1, Uzzal Kumar Das1 and Zeba I. Seraj1

Salinity limits both growths and productivity of all major crops including rice. Therefore production of salt tolerant rice is very important, particularly for Bangladesh which has large tracts of saline coastal areas. Although salinity tolerance is controlled by multiple alleles, antiporter genes have been reported to confer tolerance to dicots. We have prepared several constructs with coding regions as well as full length transcripts. These will be placed downstream of CaMV 35S promoter as available in commercial binary vectors such as pH7WG2. We have isolated a salt inducible promoter from the endogenous antiporter gene of salt tolerant landrace pokkali (PkN) which shows much stronger activity compared to CaMV 35S. We are replacing the CaMV sequence with the isolated PkN promoter in pH7WG2. Due to the lack of suitable restriction enzyme sites blunt end/ TA ligation will be attempted. In addition, we have borrowed genes from ICGEB, India such as Pennisetum glaucum antiporter (PgVNHX), helicase (PDH45) and glyoxalase I and II. Transgenic rice with PgVNHX is in T2 generation. Helicase containing rice has started to regenerate in culture. Glyoxalase I and II will be introduced individually into TOPO ENTRY vectors containing L recombination sites. These will be recombined into destination vector pH7WG2. cotransformation will be used to introduce glyoxalase I and II into rice.

1Department of Biochemistry and Molecular Biology, University of Dhaka.

133

Glutinous and Non Glutinous Rice of Bangladesh: DNA Fingerprinting and SNPs in the Waxy Gene.

Rokeya Begum1, Zeba Islam Seraj1, Abed Chaudhury2.

Most Bangladeshi rice varieties are Indica and Non Glutinous. However, Glutinous rice also exists, for example, Beruin varieties. Glutinous rice produces cohesive grains and are therefore of importance in festivals where the main food item is rice cake. Glutinous rice contains much higher level of amylopectin compared to Non Glutinous rice. This is due to lack of the synthesis of the starch amylose, because of a defect in the gene (waxy gene) for granule-bound starch synthase. This defect is due to G to T mutation, which causes defective splicing of the gene transcript. The amylose content in Non Glutinous rice however varies considerably, apparently because of the suppression of the waxy gene mutation due to sequences elsewhere. This has been shown by an analysis of rice genotypes from East Asia. We are sequencing the waxy gene from Non Glutinous rice’s of Bangladesh in an attempt to find whether they also contain the waxy gene mutation and are therefore showing suppression. We are also sequencing Glutinous rice varieties to confirm the presence of the waxy gene mutation. In addition, we are doing rice microsatellite fingerprinting of Non Glutinous and Glutinous rice varieties. Such a study may detect the presence of SNPs indicative of the origin of Bangladeshi rice. 1Department of Biochemistry and Molecular Biology, University of Dhaka. 2CSIRO, Australia

134

Rice Transformation with Antiporter Genes and Their Expression with Strong Promoters

Rumana Sultana Tammi1, Sharmin Jahan2, Lisa Perveen2, Laisa A. Lisa3, Noorain M.

Rasul2 and Zeba I. Seraj2

Transgenic rice with the rice vacuolar Na/H antiporter gene (vOsNHX1) driven by the CaMV 35S and actin promoters has been produced. PCR-positive T0 plants were selfed to T1 and T2 and screened for seedling salinity tolerance in hydroponics. At least four transgenic lines showed greater tolerance scores compared to untransformed controls. Semi-quantitative RT-PCR results correlated with seedling tolerance scores with tolerant lines showing greater expression of the vOsNHX1 transcript. The endogenous gene also showed poor expression compared to transformed tolerant as well as those which showed poor tolerance scores. The endogenous promoter for the OsNHX1 gene for the salt tolerant landrace Pokkali shows several fold higher activity compared to either CaMV 35S or actin promoters in promoter GUS transient assays of rice calli. Plants are being transformed with the promoter-GUS constructs to check expression and salt inducibility in intact plants. 1Current affiliation: Department of Biochemistry and Molecular Biology, Jahangir Nagar University 2Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000 3Department of Biochemistry and Molecular Biology, University of Rajshahi

135

Functional Analysis of GAMYB Gene Involved in Gibberellin Signaling in Rice

S.M. Shahinul Islam1, Takahiro Miyazaki3, Yoshito Tanaka2, Kan Nishimura3, Toshiaki

Mitsui 2,3, Shuji Yokoi4, Ko Shimamoto4, Kimiko Itoh2,3 GAMYB is a R2R3 type Myb related transcription factor and activates GA inducible á-amylase gene during cereal seed germination. Recent studies reported that GAMYB may be involved in floral initiation, internodes elongation and anther development. We isolated OsGAMYB mutant (OsGAMYBm) which showed lower expression of OsGAMYB and remarkable decreasing number of rachis branch. We assumed that engineering of OsGAMYB gene expression may enable for production of high yielding rice by controlling number of rachis and floret. OsGAMYB expression was genetically engineered by introduction of sense cDNA or RNAi construct into rice cv. Toride-1. Resulting OsGAMYB disruptant showed decreased plant height, number of primary branch and early flowering like as OsGAMYBm. By contrast, OsGAMYB overexpression showed late flowering and increased number of caryopsis. The increasing of caryopsis numbers may affect yielding of rice. But overexpressor of the OsGAMYB also led to less seed fertility. In this study we also analyze the phenotype and GA responsiveness in mutant. Wild type (WT) and mutant (MT) plants were grown with or without GA3 treatment. It was observed that plant height of MT lower than WT. So, the OsGAMYB promotes leaf elongation. We also observed the effect of GA3 on plant height. GA3 treated WT showed strong internodes elongation than non-treated WT. And GA3 treated MT showed slightly increased plant height than non-treated MT plant. But not recovered their dwarfism completely. So, the mutant remained partial GA responsiveness at adult phase. 1: Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh 2: Graduate School of Science and Technology, Niigata University, Ikarashi-2, 950-2181 Niigata, Japan 3: Faculty of Agriculture, Niigata University, Ikarashi-2, 950-2181 Niigata, Japan 4: Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-01, Japan

136

Evaluation of Salt Tolerance in Rice Using Phenotypic and Marker-Assisted Selection

S. K. Bhowmik1*, M. M. Islam2, R. M. Emon2, S. N. Begum2, A. Siddika1 and S. Sultana1

Eleven rice genotypes were used to evaluate salinity tolerance through phenotypically and genotypically. Recent advent of molecular markers particularly, microsatellite or simple sequence repeats (SSRs) were used for the marker-aided selection (MAS) to find out salinity tolerance in rice. Three selected SSR primers viz., RM7075, RM336 and RM253 were used to evaluate rice genotypes for salt tolerance. Two setups were maintained for this study viz., seedling stage and reproductive stage. Phenotyping of 11 genotypes at seedling stage was done in hydroponic system using salinized (EC 12 dS/m) nutrient solution and at the reproductive stage using salinized tap water (EC 6 dS/m). IRRI standard protocol was followed to evaluate salinity tolerance. The effect of prolonged salinity stress on yield and yield components of 11 rice germplasms at both stages was evaluated. At seedling stage, average reduction in plant height and total dry matter of tolerant lines were reduced by 19.0% and 40.6%, respectively in salinized condition where as those of susceptible lines were reduced by 46.0% and 73.5%, respectively. Similarly at reproductive stage, tolerant genotypes showed lower reduction in terms of plant height, total dry matter and number of filled grains (8.96%, 27.04% and 20.6%, respectively) and susceptible lines showed higher reduction (17.1%, 61.05% and 80.9%, respectively). The markers showed polymorphism and were able to discriminate salt tolerant genotypes from susceptible. The genotypes having similar banding pattern with Pokkali were considered as salt tolerant. The SSR markers RM7075, RM336 and RM253 identified 8, 9 and 7 genotypes, respectively as salt tolerant. Through phenotypic and genotypic study, five genotypes viz., Pokkali, Dhol Kochuri, RD-2586, TNDB-100 and PNR-519 were identified as salt tolerant rice cultivar. These SSR markers might have sequence homology with salt tolerant rice genotypes and consequently the markers could able to identify salt tolerant genotypes from susceptibles. Key words: Rice, Salt stress, growth stages, SSR markers and MAS. 1Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh 2Bangladesh Institute of Nuclear Agriculture, P.O. Box 4, Mymensingh 2200, Bangladesh. Name of corresponding author: S. K. Bhowmik Email: [email protected]

137

Effects of Black and Green Tea Extracts (Polyphenols) on Arsenic-Induced Toxicity in Rabbits

Sheikh Zahir Raihan1, A. K. Azad Chowdhury1, Mohammad Shawkat Ali1,

Farzana Marni2, G.H. Rabbani2 Arsenic causes oxidative stress in the body. Unfortunately there are no effective drugs for its chronic toxicity. Tea polyphenols are potent antioxidant. It’s of interest to evaluate the effect of tea polyphenols on the oxidative stress from arsenicosis. The study was conducted at the Clinical Research and Service Centre of ICDDR,B: Centre for Health and Population Research and at Pharmacology Laboratory of the Department of Clinical Pharmacy and Pharmacology, University of Dhaka. In this trial, 20 rabbits (1.94 and 2.86 kg weight) received arsenic trioxide (3 mg/kg/day) for 14 days which caused a significant reduction of whole blood glutathione (GSH) and elevation of thiobarbituric acid reactive substances (TBARS) and the index of nitrite/nitrate (NOx) levels. The institutional ethical guidelines were followed for all animal experiments. Black and green tea extract (polyphenols) administration for the next 14 days to the appropriate groups caused a significant elevation of depleted GSH levels and reduction of TBARS levels compared to post-arsenic levels. GSH concentrations in intoxicated rabbits were increased from 19.8 ± 1.4 (post-arsenic value) to 30.3 ± 4.2 mg/dL (P = 0.04) in the black tea group, from 20.4 ± 1.5 to 32.2 ± 2.9 mg/dL (P = 0.002) in the green tea group and from 20.8 ± 2.9 to 26.4 ± 3.4 mg/dL (P = 0.23) in the placebo group. TBARS levels in intoxicated rabbits were decreased from 4.1 ± 0.8 (post-arsenic value) to 2.37 ± 0.2 ìM/L (P = 0.05) in the black tea group, from 4.7 ± 0.8 to 1.9 ± 0.2 ìM/L (P <0.005) in the green tea group and from 4.1 ± 0.3 to 3.2 ± 0.3 ìM/L (P = 0.08) in the placebo group. The polyphenols components of black and green teas were 27.69% and 29.71% of the dry weight of the total extracts respectively. These results indicate that arsenic induced toxicities in rabbits were significantly reversed by the black and green tea extracts. The greater activity of green tea compared to that of black tea correlates with slightly higher content of polyphenols in green tea. Key words: Arsenicosis; oxidative stress; black tea; green tea; polyphenols. 1Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh. 2ICDDR,B, GPO Box 128, Dhaka 1000, Bangladesh Name of corresponding author: Professor Dr. A. K. Azad Chowdhury Email: akazad@univdhaka, [email protected], Fax: +880-2-8615583

138

Exocrine and Endocrine Pancreatic Function in T2d Patients of Bangladesh

S. Sattar1, C. Hanck2, Z. Hassan1, U. Keller3, D. Whitcomb4, L. Ali5,

A. K. Azad Khan6, N. Gyr7; Aims: Exocrine pancreatic dysfunction in T2D is still an unsettled issue. Since SPINK1 gene N34S variant has been associated with pancreatopathy we aimed to analyze the gene variant and evaluate the exocrine and endocrine functional status in a cohort of typical T2D patients in comparison to tropical calcific pancreatitis patients with and without diabetes.

Methods: Age-matched T2D patients (n=49), tropical calcific pancreatitis without diabetes (TCP, n=35) and with diabetes [Fibrocalculus calculus pancreatic diabetes (FCPD), n=83)] were studied. Pacreatic exocrine function was evaluated by measurement of fecal elastase1 (by ELISA) and endocrine by measurement of insulin (by RIA) following IV agrinine stimulation. SPINK1 gene N34S variant was analyzed by direct DNA sequencing. Student’s unpaired-‘t’, Mann-Whitney and Chi-square tests were performed where applicable.

Results: Fecal elastase1 was severely (<100 ìg/g of stool) reduced in 58% of T2D subjects compared to 81% (p=0.082) of TCP and 95% of FCPD subjects(p=0.001). SPINK1 N34S variant was positive in 8.8% of T2D, 37% TCP (p=0.011) and 33% FCPD (p=0.011) subjects. The N34S variant positivity was present in subjects with severely reduced Fecal elastase1 of 15% of T2D, 18% TCP and 39% FCPD subjects. In all three groups SPINK1 N34S variant positive and negative cases did not show any difference regarding Fecal elastase1. Insulin levels (median, U/l) at basal and on arginine stimulation (at 15, 30, 45 and 60 min) in T2D subjects (basal- 24.4, stimulated- 41.7, 42.3, 34.3 and 21.9) was significantly higher compared to TCP (basal- 13.0, stimulated- 21.6, 23.4, 18.7 and 14.0) and FCPD subjects (basal- 15.9, stimulated- 20.7, 19.5, 22.5 and 15.1). Between SPINK1 N34S variant positive and negative cases insulin levels at basal and stimulated state did not show any statistical difference.

Conclusion: The data suggest that i) a substantial proportion of T2D patients in Bangladesh have exocrine pancreatic dysfunction; ii) in T2D the exocrine dysfunction and insulin secretory capacity is not associated with SPINK1 gene variant. 1Dept of Physiology and Molecular Biology, BIRDEM, Dhaka, Bangladesh, 2Dept of Gastroenterology, University Hospital, Basel, Switzerland, 3Dept of Endocrinology and Metabolism, University Hospital, Basel, Switzerland, 4Centre for Genomic Sciences, University of Pittsburgh, Pittsburgh, PA, United States, 5Dept of Biochemistry and Cell Biology, BIRDEM, Dhaka, Bangladesh, 6Dept of Gastroenterology, BIRDEM, Dhaka, Bangladesh, 7Dept of Internal Medicine, University Hospital, Basel, Switzerland.

139

Expression and Purification of a Ruler Protein, gp29, of Bacteriopahge T4 by two-step affinity chromatography and its identification by

MALDI-TOF MS

Subodh Kumar Sarkar#, Shuji Kanamaru* and Fumio Arisaka* In order to express the “ruler protein”, gp29, of bacteriopahge T4, gene 29 histidine-tagged at the N-terminus was cloned into a double promoter-based expression vector, pQE32, and over-expressed in E coli SG13009 under osmotic stress in the presence of sorbitol and betaine. Following expression, it was purified by immobilized metal affinity chromatography and immuno-affinity chromatography after ammonium sulphate precipitation. The purified protein migrated on SDS-PAGE with an apparent molecular mass of 80 kDa. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was used to confirm the identification of gp29 using peptides derived from the protein after in-gel digestion with trypsin. Keywords: Bacteriophage T4; Ruler molecule; Affinity chromatography; Mass spectrometry; MALDI-TOF MS

* Department of Bimolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku,Yokohama 226-8501, Japan. # Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong(USTC), Foy’s lake,

Chittagong-4202, Bangladesh.

140

Zinc Supplementation of Pregnant Rats with Adequate Zinc Nutriture Suppresses Immune Functions in Offspring1

Rubhana Raqib2, Taher Uddin2, Mohammad Bakhtiar Hossain2,3, Shannon L Kelleher3,

Charles B Stephensen3,4, Bo Lönnerdal3

Background: Zinc (Zn) that takes part in hundreds of vital enzymes function in every stage of life shows significant effects in neonatal in addition to their following progeny in immune response if deficient in preconception, gestational, lactation, and weaning stages. Zn supplementation can partially restore the problems that is established both in human and animal model system. But over dose of Zn to Zn adequate mother can demonstrate suppressive outcome and impair immune function in their offspring. Objective: The aim of this study to create concern in vulnerable people as Zn has a noxious effects on immune system if supplemented in Zn adequate mother and so it should not be given without examine the level. Methods: Adult female rats were fed a Zn-adequate diet (ZC, n=8) or a Zn-deficient diet (ZD, n=8) from preconception through lactation. After birth, the pups were designed as follows: ZCZ+, pups of control dams received Zn-supplimentation; ZCZ-, pups of control dams that received placebo; ZDZ+, pups of Zn-deficient dams that received Zn supplementation; ZDZ-, pups of Zn-deficient dams that received placebo. Pregnant rats were supplemented with either Zn (1.5 mg Zn in water) or placebo (water) 3 times/ wk throughout pregnancy. Pups were orally immunized with cholera toxin and bovine serum albumin-dinitrophenol (DNP) 3 times at weekly intervals and killed 1 wk after the last dose. Proliferation and cytokine responses in lymphocytes from Payer’s patches (PP) and spleen, and antigen specific antibodies in serum were studied as well as the thymus weight. Results: Zn supplementation in Zn adequate dams show suppressive response in lymphocyte proliferation and IFN-ã whereas deficient dams show vise versa relationship. Humoral immune responses in pups were depressed in Zn-adequate plus Zn-supplemented dams though it neither shows reinstallation in Zn-deficient mother if supplemented. Conclusion: So far there is no appropriate marker to measure the Zn-level, mothers should not be supplemented arbitrarily during pregnancy. 1The study was conducted with the support from Ellison Medical Foundation at the Department of Nutrition, University of California Davis. 2ICDDR,B: Centre for Health and Population Research, Dhaka, Bangladesh; 3Department of Nutrition, University of California; 4USDA Western Human Nutrition Research Center, Davis, CA, USA. *Corresponding author: ([email protected])

141

Chromatography Paper Strip Method for Collection, Transportation, and Storage of Viral Genetic Material in Stool Samples

M. Rahman1, T. Azim1, G. Podder1, P. Maes2, K.T. Zlateva2,

E. Wollants2 and M. Van Ranst2 In many epidemiological and vaccine trials, samples need to be transported to reference laboratories for analysis. Often this involves shipping samples under cooled and biohazardous conditions. Even the simple acts of sample collection, aliquoting, and freezing might be difficult in field situations in some areas in developing countries where no electricity or skilled laboratory technicians are available. We describe the development of a novel method that allows the collection, transport, and storage of genetic materials on a solid chromatography paper support. The chromatography strips were pretreated with SDS and EDTA and were infected with positive stool samples for rotavirus, adenovirus, norovirus and poliovirus. The infected strips were stored at -20°C, 4°C, room temperature (20 to 25°C), and 37°C. The presence of viral genetic material on the strips was detected by virus-specific PCR amplification after storage for different time periods (between 7 to 120 days). Biosafety experiments were performed to check if the viral particles were still infectious after contact with the SDS/EDTA-pretreated paper strips. The genetic materials collected on paper strips were successfully detected by PCR after four months storage at all temperatures. This finding indicates that viral RNA and DNA on the paper strips are stable for a sufficient amount of time at room temperature or even in warmer climatic conditions. Furthermore, the PCR product from the chromatography strip was successfully sequenced, indicating that the sampling method can be used for further genetic characterization. No viral infectivity was observed upon contact with the SDS/EDTA-pretreated strips. Thus the strips can be used in a safe way. In conclusion, the SDS-EDTA-pretreated chromatography filter paper strips are a convenient, safe, and inexpensive method to collect, store, and transport samples for detecting, typing and further genetic characterization. Key words: Chromatography Paper Strip, Rotavirus; Norovirus; Adenovirus; Poliovirus; DNA, RNA, Storage, Collection, Transportation 1ICDDR,B, GPO Box 128, Dhaka 1000, Bangladesh 2 Laboratory of Clinical Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium. Name of corresponding author: Dr. Tasnim Azim Email: [email protected] Fax: 88-02-8823116

142

Expatriate and Foreign Scientists Messages and Abstracts

143

MESSAGE I am very happy that a conference on the “Promotion of biotechnology in Bangladesh: National and International Perspectives” is being held under the sponsorship of Ministry of Science, Information and Communication Technology, Bangladesh Academy of Science, Dhaka University, BRAC University, ICDDR,B, Square- and Incepta Pharmaceuticals. Bangladesh is rich in biodiversity which constitutes the feed stock for biotechnology research and application. I hope the conference will provide a road map for getting the farmers and people of Bangladesh the benefits of the new genetics. I also hope that the conference will help in promoting the responsible and beneficial use of biotechnology. The bottom line of our national agricultural biotechnology policy should be the economic well being of farm families, food security of the nation, health security of the consumer, protection of the environment and the security of national and international trade in farm commodities. I wish the conference great success. Prof M S Swaminathan President, Pugwash Conferences on Science and World Affairs Chairman, M S Swaminathan Research Foundation Third Cross Street, Taramani Institutional Area Chennai - 600 113 (India) Tel: +91 44 2254 2790 / 2254 1229; Fax: +91 44 2254 1319 Email: [email protected] / [email protected]

144

Use of Post-Genomic Technologies for Drug Target

Discovery and Validation

Error! Reference source not found., PhD The current trend in drug discovery reflects a paradigm shift from conventional to genomics-based target identification. The publication of the human genome sequence as well as the availability of some exciting new technologies has generated a wealth of new gene targets. However, most of the identified genes lack functional information that is required for proper target validation. We discuss three approaches to undertake disease gene identification and validation currently used in our laboratory:

1. Functional analysis of microarray-generated target gene identification; 2. siRNA-mediated gene silencing to determine proof-of-principle; 3. Bioinformatics-based analysis of gene expression profiles to determine regulatory

networks.

In an integrated model of innate immunity against cancer and infection, NK cells play an important role. Ability of NK cells to eliminate tumor cells depends on: (a) lack or reduced level of MHC class I on the tumor cells, (b) strength of NK cell/tumor cell binding, frequency of the MHC class I-specific inhibitory receptors, (c) phenotype and the frequency of NK subsets, (d) types and level of released cytokines and chemokines by activated NK cells. We have used high throughput, antibody based cytokine chips to study the levels of secreted cytokines/chemokines production by CD16 and 2B4 cross-linked activated NK cells. Regulation of NK function depends on recruitment, renewal and differentiation of lymphocytes such as neutrophils, and intrinsic factors such as cytokines/chemokines and adhesion molecules, intrinsic control by transcription factors and their target genes. Analysis of cell network connectivity takes a center stage in determining ‘robust innate immunity’ against cancer. Error! Reference source not found., PhD CBRIBR Department of Pathology Harvard Medical School 800 Huntington Ave, Boston, MA 02115 [email protected]

145

Can NIB Facilitate the Birth of Interdisciplinary Research in Bangladesh?

Parvez I. Haris It is widely accepted that complex biological problems cannot be solved by pure disciplinary research and this has led to the emergence of the exciting paradigm of interdisciplinary research. Undoubtedly, we are entering a new era in scientific research where the ideas and technologies of life science, physical science and information science are rapidly merging. Already in many parts of the world science policies and existing institutional structures are being changed to meet this reality to speed up scientific discovery through interdisciplinary research. This presentation is based on research on the level of interdisciplinary research in Bangladesh through an analysis of the journal publications emerging from Bangladesh over a period of 36 years. This research has revealed that interdisciplinary research in Bangladesh is virtually absent. For example, collaboration between Biochemistry and Chemistry Departments, within an institution and between institutions, in majority of the cases is either non-existent or negligible. Yet, individuals from these Departments do engage in interdisciplinary research with overseas collaborators. Overseas funding is often the incentive that underpins such interdisciplinary research. Two major factors appear to be responsible for the lack of interdisciplinary research in Bangladeshi Universities. One is lack of incentives; especially the lack of funding for such types of research. The other major barrier is the existing departmental structure that creates insularity and mistrust. Clearly, a change in the structure and environment in universities is required such that interdisciplinary work is rewarded in the form additional staffing, research infrastructure and funding. This should be the long term objective. Meanwhile, the establishment of the NIB provides an excellent opportunity for giving birth to interdisciplinary research which should not be missed. This is particularly important since the National Biotechnology Policy document has already come under criticism from some scientists who strongly feel their discipline has been left out or marginalised [1]. To avert such divisive atmosphere amongst the scientific community, and at the same time nurture high quality science, the NIB should initiate important and exciting research projects that are inherently interdisciplinary. This will help build bridges between different disciplines, individual scientists and will also simultaneously advance scientific progress across the respective disciplines. The NIB should aim to be a home for creative scientists, experts in their respective disciplines, who come together united to tackle important and challenging research problems relevant to Bangladesh. There are several health, pharmaceutical, environmental, agriculture and food related problems in Bangladesh that can easily form the nucleus for initiating such interdisciplinary research. The presentation will specifically focus on some specific areas of research that are inherently interdisciplinary. It will also give examples, from the authors own interdisciplinary research, spanning over two decades, which has ranged from basic science including biochemistry, biophysics, immunology, proteomics and bioinformatics to development of biomaterials and novel antimicrobial strategies. Parvez I. Haris Faculty of Health & Life Sciences, De Montfort University, Leicester, LE1 9BH, United Kingdom, E-Mail: [email protected] Reference: [1] Minutes of the First Workshop on National Biotechnology Policy Document, Dhaka, December 2006.

146

Questionnaire

Expatriates Local Scientists Oral Presenters

Poster Presenters

147

Md Abdul Khaleque, Ph.D A. Biosketch of Participants: 1. Name with academic title

Md Abdul Khaleque, Ph.D 2. Current position with affiliation, address and contact details

Scientist (Technical), Zymed Division Invitrogen Corporation (www.invitrogen.com) 542 Flynn Road Camarillo, California 93012 USA Tel: 1-805-484-5593 (r), 1-805-824-2575 (c) Email: [email protected] [email protected]

3. Immediate past position(s)

Research Fellow Beth Israel Deaconess Medical Center Harvard Medical School Department of Radiation Oncology 21-27 Burlington Ave. Boston, Massachusetts 02215 USA

4. Graduate and postgraduate degrees and training

Degrees: Kumamoto University School of Medicine, Kumamoto, Japan Ph.D., Molecular Genetics, March 2002 Dissertation title: Studies on a putative mitochondrial protein import factor metaxin and a new Hsp70 cochaperone dj4. Advisor: Prof. Masataka Mori University of Dhaka, Dhaka, Bangladesh Master of Science, Biochemistry and Molecular Biology, 1991 (held in 1994) Dissertation title: Changes in lipid profile and platelet aggregation in smokers of different duration. Advisor: Prof. Ishtiaq Mahmud University of Dhaka, Dhaka, Bangladesh Bachelor of Science, Biochemistry and Molecular Biology, 1990 (held in 1992) Training:

Harvard Medical School, Beth Israel Deaconess Medical Center, Department of Radiation Oncology, Boston, MA Postdoctoral Fellow (with Dr. SK Calderwood), Dec 2003 – July 2006

Train and supervise graduate students and technicians in various technical aspects of molecular biology, biochemistry, mammalian tissue culture, signal transduction etc. My post-doctoral studies are directed at understanding the role of heat shock protein (HSP) in cancer metastasis. I found that heat shock factor 1 (HSF1) binds to metastasis protein and promotes pro-metastatic changes through heregulin-dependent gene expression. My present research is on the mechanisms underlying the elevation of HSP in cancer involving the oncogenic protein heregulin and its signaling receptor c-erbB. In addition, I am studying the role of HSP70 in inhibiting programmed cell death in breast and prostate cancer cells. This study is focused on the role of the heat shock response in cancer and studying the use of HSP in the design of anticancer vaccines. Overall, these studies will help us to understand more on gene expression and regulation in cancer models, tumor progression and

Expatriates

148

metastasis. Boston University Medical Center, Center for Molecular Stress Response, Boston, MA Postdoctoral Fellow (with Dr. Calderwood), Nov 2002 - Nov 2003

Supervise graduate/under graduate students and technicians. I was involved with several contemporary research projects related to the cancer biology, HSF1, HSP and the regulation of protein phosphorylation in signal transduction pathways. My key research was on the process of metastasis, a key event in the progression of cancer. My observations suggested for the first time that metastasis associated protein 1 (MTA1) interacts with HSF1 and promotes pro-metastatic changes through the c-erbB signaling pathway. These findings opened a new area of research involving heat shock proteins in cancer progression mechanisms. National Health Care Network (NHN), Department of Biochemistry and Endocrinology, Dhaka, Bangladesh Scientific Officer, Feb 1996 – Sept. 1997

Conducted research on clinical, biochemical and endocrinological tests. Samples were from different kinds of diabetic patients from all over the country. Data were mainly used for the clinical treatment and research purpose. I was also involved in a research project titled "Acute effect of a low calorie ice cream on glycemic status and atherogenic risk factors in NIDDM subjects".

International Center for Diarrhoeal Disease Research (ICDDRB), Biochemistry and Nutrition Lab, Dhaka, Bangladesh Research Officer, Oct 1995 - Jan 1996

I had the opportunity to work on the projects “Vitamin-A metabolism in childhood infection” and “Content of �-carotene and ratinol in different food samples”. From these projects I gained experience in various methods like, determination of Vitamin-A from food samples, breast milk, serum and urine; �-carotene from food samples by using HPLC, determination of Ratinol Binding Protein and Pre-albumin by Radial Immuno-diffusion technique and determination of trace elements from serum by Absorption Spectrophotometric method.

Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Department of Cell & Molecular Biology, Dhaka, Bangladesh Research Fellow, June 1994 - Sept 1995 I learned advanced methods in biochemistry, pharmacology and cell biology such as insulin release from isolated islets, preparation of single �-cell and measurement of insulin by ELISA, platelet aggregation measurement, cell separation and culture. In addition, I assisted in the measurement of cytoplasmic Ca+2 in single living cells by dual wave-length fluorimetric system using Fura-2 as the fluorescent indicator.

5. Prizes/Awards/Honours (academic and research)

BIDMC Oncology Fellowship, Harvard Medical School, Boston, MA (2005-2006) Young Investigator Travel Award from Society for Thermal Medicine (2005) NIH Postdoctoral fellowship (CA47407, CA31303 and CA50642) through Dr. Stuart Calderwood (2002-2006) Monbusho Scholarship from Ministry of Education and Science, Japan (1997-2002) HDF scholarship under Talent Assistance Scheme, Bangladesh (1986-1994) Begum Halima Khatun Scholarship from University of Dhaka, Bangladesh (1990) Chancellor award from the President of the Peoples Republic of Bangladesh (1987)

6. Elected Fellowships of Academies

Research Advisory Positions/Journal Editorship/Directorship of Boards Membership of Institutional Committees

Founder and General Secretary, Enviroprotection and Human Development (Environmental NGO), Dhaka, Bangladesh (www.angelfire.com/ak/EPHD)

149

Member, Cell Stress Society International, USA (www.cellstress.uconn.edu) Member, Society for Thermal Medicine, USA (www.thermaltherapy.org) Associate Member, American Association for Cancer Research, USA (www.aacr.org) Member, Global Network of Bangladeshi Biotechnologists, USA (www.gnobb.org) Member, Graduate Biochemists Association, Bangladesh (www.gbabd.org) Director, Family Needs Ltd (Supermarket), Uttara, Dhaka, Bangladesh (www.familyneeds-bd.com)

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research

I have been working on the role of the molecular chaperone heat shock proteins (hsp) in the progression of cancer and cancer therapy. Hsp are highly conserved stress proteins whose expression confers resistance to a number of types of cancer therapy. They function by promoting protein repair as well as being direct antagonists of effector steps in apoptosis and other forms of cell death. Many of the regulatory steps in hsp gene expression, including the transcriptional and posttranscriptional levels that permit the massive expression of the hsp in stress situations and protection from apoptosis and other forms of cell death has been elucidated. My studies are directed at understanding the role of heat shock protein (HSP) in cancer metastasis. I found that heat shock factor 1 (HSF1) binds to metastasis protein and promotes pro-metastatic changes through heregulin-dependent gene expression. Recently I completed a research on the mechanisms underlying the elevation of HSP in cancer involving the oncogenic protein heregulin and its signaling receptor c-erbB. In addition, I studied the role of HSP70 in inhibiting programmed cell death in breast and prostate cancer cells. My studies on this process have begun to bear fruit, leading to the development of drugs tailored to inhibit HSP70 and HSF1 function and enhance tumor control. I have planed to focus on the role of the heat shock response in cancer and studying the use of HSP in the design of anticancer vaccines. I also have begun to probe the regulation of HSF1 and HSP expression in cancer cells using humanized monoclonal antibody (2C4 and herceptin) and various pharmacological agents. These will help us to understand more on gene expression and regulation in cancer models, tumor progression and metastasis.

2. Area(s) of Expertise

Molecular Biology:

All general molecular biology techniques including but not limited to: Genomic DNA isolation from tissue and cultured cells, plasmid DNA preparation, cDNA library construction, cDNA and genomic DNA library screening and clone isolation, DNA sequencing, RNA isolation from whole tissue and cultured cells, PCR for various application including site directed mutagenesis and RT-PCR, silencing of genes using RNAi, northern blots and western blots, chromatin immunoprecipitation (ChIP) assay.

Molecular Pathology:

CISH (Chromogenic In-situ Hybridization), FISH (Fluorescence In-situ Hybridization), IHC (Immunohistochemistry), IF (Immunofluorescence)

Biochemistry:

Protein expression and purification, SDS-PAGE, Blue native PAGE, ELISA and protein import into mitochondria, techniques required for protein phosphorylation and signal transduction mechanism.

Tissue culture/ cell based assay:

Mammalian cell culture (HeLa, MCF-7, CHO-K1, MDA-MB-231, MEF, H9c2 and others), transient and stable transfection, gene expression and characterization, anchorage independent growth assay.

Other skills:

Luciferase assay, apoptosis assay, broad experience in signal transduction steps, tumor progression and metastasis.

3. Number of peer-reviewed publications: 9 Full paper, 3 Book chapter, 1 Environmental article

150

4. List of 5-10 most significant publications

• Md Abdul Khaleque, Ajit Bharti, Jianlin Gong, Daniel R. Ciocca, Arturo Stati, Mariel Fanelli and Stuart K. Calderwood. Heat Shock Transcription Factor 1 Represses Transcription Through Association with Metastasis Associated Protein 1. Oncogene. (2006). MS in review

• Yutaka Enomoto, Ajit Bharti, Md Abdul Khaleque, Baizheng Song, Chunlei Liu, Vasso Apostolopoulos, Pei-xiang Xing, Stuart Calderwood and Jianlin Gong. Enhanced immunogenicity of heat shock protein 70 peptide complexes from dendritic cell-tumor fusion cells. J Immunol. 177: 5946-5955. (2006).

• Stuart K Calderwood, Md Abdul Khaleque, Douglas B. Sawyer and Daniel R. Ciocca. Heat shock proteins in cancer: chaperones of tumoregenesis. Review. Trends Biochem Sci.31(3):164-72. (2006)

• Md Abdul Khaleque, XiaoZhe Wang, Mei Juan Zhau, Rong Zhong, Matthias Gaestel and Stuart K Calderwood. Phosphorylation of HSF1 by MAPKAP Kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding. J Biol Chem. 281(2):782-91. (2006). Equal contribution

• Md Abdul Khaleque, Ajit Bharti, Douglas Sawyer, Jianlin Gong, Ivor J. Benjamin, Mary Ann Stevenson and Stuart K. Calderwood. Induction of heat shock proteins by heregulin �1 leads to protection from apoptosis and anchorage-independent growth. Oncogene, 24, 6564-6573 (2005).

• Dan Tang, Md Abdul Khaleque, Ellen L. Jones, Jimmy R. Theriault, Cheng Li, Wing Hung Wong, Mary Ann Stevenson and Stuart K. Calderwood. Expression of heat shock proteins and HSP mRNA in human prostate carcinoma in vitro and in tumors. Cell Stress & Chaperones 10 (1), 46-58 (2005).

• Donghui Yu, Ehsan Khan, Md Abdul Khaleque, James Lee, Gary Laco, Glenda Kohlhagen, Surender Kharbanda, Yung-Chi Cheng, Yves Pommier and Ajit Bharti. Phosphorylation of DNA Topoisomerase I by the c-Abl tyrosine kinase confers camptothecin sensitivity. J Biol Chem. 279(50):51851-61 (2004).

• Khaleque Md Abdul, Kazutoyo Terada, Tomomi Gotoh, Rahman Md. Hafizur, Masataka Mori. Characterization and functional analysis of a heart-enriched DnaJ/Hsp40 homolog dj4/DjA4. Cell Stress & Chaperones 7(2), 156-166 (2002).

• Khaleque Md Abdul, Kazutoyo Terada, Masato Yano, Michael T. Ryan, Illo Streimann, Nicholas J. Hoogenraad, Masataka Mori. Functional analysis of human metaxin in mitochondrial protein import in cultured cells and its relationship with the Tom complex. Biochem Biophys Res Commun 276, 1028-1034 (2000).

5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained 7. Size and qualifications of research team supervised 8. National and International Collaborations 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh: (Information requested from

Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? I would like to return Bangladesh permanently and do competitive research in any institute which

would offer me modern research facility in an attempt to address some of the pressing problems facing the country like cancer, effect of arsenic on health etc. I have extensive practical experience with molecular- and cancer biology and looking for a prospective position in Cancer Biology/ Biotechnology/ Environmental Science/ Epidemiology/ Clinical diagnosis.

151

6. Any other comments?

Dr. Abidur Rahman A. Biosketch of Participants:

1. Name with academic title: Dr. Abidur Rahman 2. Current position with affiliation, address and contact details:

Assistant Professor Cryobiosystem Research Center Faculty of Agriculture Iwate University Ueda 3-18-8; Morioka 020-8550 Japan Tel/fax:+81-19-621-6144 Email: [email protected] Lab url: http://news7a1.atm.iwate-u.ac.jp/~abidur/

3. Immediate past position(s) Senior Postdoctoral Researcher (2003-2006) Biology Department, University of Massachusetts,

Amherst, USA Postdoctoral Researcher (2002) Japan Atomic Energy Research Institute, Takasaki, Gunma, Japan 4. Graduate and postgraduate degrees and training M.Sc. Department of Biochemistry, Dhaka University Ph.D Graduate School of Science and Technology, Kobe University 5. Prizes/Awards/Honours (academic and research) Recent article on a new auxin mutant of Arabidopsis was selected as a featured article by the editor-in

chief of Plant Journal (2006) Best poster award for young emerging scientist by Japanese botanical society (2002) 6. Elected Fellowships of Academies

Postdoctoral fellowship awarded by Japanese society for promotion of Science (2002) Grant-in-aid fellowship by the ministry of education, Japan Govt. for completing the Ph.D (1997-2001)

University merit scholarship (1989-1993) 7. Research Advisory Positions/Journal Editorship/Directorship of Boards Serve as a reviewer for the journals Plant and Soil ; Plant and cell physiology 1. Membership of Institutional Committees Member of academic committee, Faculty of Agriculture, Iwate University B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research

Hormonal response on growth and development of plant. My lab is currently focused on elucidating the novel transport and response mechanism of auxin in Arabidopsis. We are also interested in dissecting the mechanism of auxin-ethylene crosstalk in plants. In addition, we recently initiated a project exploring the possibility of developing a bio marker for arsenic detection inside the cell and to elucidate new proteins involved in transport of arsenic.

2. Area(s) of Expertise Plant physiology, Molecular Biology, Cell biology, Protein chemistry

3. Number of peer-reviewed publications 4. List of 5-10 most significant publications

1. Rahman A, Bannigan A, Sulaman W, Pechter P, Blancaflor EB, Baskin TI (2007a) Auxin, actin, and growth of the Arabidopsis thaliana primary root.

Plant Journal (In press)

Expatriates

152

2. Muday G K, Rahman A (2007b) Auxin transport and the integration of gravitropic growth. In Tropism, (Gilroy S and Masson P, ed.). Blackwell Publishing, MA, U.S.A. (In press).

3. Rahman A, Nakasone A, Chhun T, Ooura C, Biswas KK, Uchimiya H, Tsurumi S, Baskin TI, Tanaka A, Oono Y (2006) A novel protein, SMAP1, mediates responses of the arabidopsis root to the synthetic auxin 2,4-dichlorophenoxyacetic acid.

Plant Journal 47:788-801 4. Swarup R, Kargul J, Marchant A, Zadik D, Rahman A, Mills R, Yemm A, May S, Williams L, Millner P, Tsurumi S, Moore I, Napier R, Kerr ID, Bennett MJ (2004) Structure-Function Analysis of the presumptive Arabidopsis Auxin Permease AUX1.

Plant Cell 16:3069-3083 5. Oono Y, Ooura C, Rahman A, Aspuria ET, Hayashi KI, Tanaka A, Uchimiya H (2003a) p-Chlorophenoxyisobutyricacid impairs auxin response in Arabidopsis root.

Plant Physiol. 133: 1135-1147 6. Ahamed A, Rahman A, Hayashi F, Ueji S, Amakawa T, Tsurumi S (2003b) Isolation of

chromosaponin I-specific antibody by affinity chromatography. Biochemical and Biophysical Research Communication (BBRC) 302: 587-592 7. Rahman A, Hosokawa S, Oono Y, Amakawa T, Goto N, Tsurumi S (2002) Auxin-and ethylene-

response during Arabidopsis root hair development dissected by auxin influx modulators. Plant Physiol. 130: 1908-1917 8. Rahman A, Ahamed A, Amakawa T, Goto N, Tsurumi S (2001a) Chromosaponin I specifically

interacts with AUX1 protein in regulating the gravitropic response of Arabidopsis roots. Plant Physiol. 125:990-1000 9. Rahman A, Amakawa T, Goto N, Tsurumi S (2001b) Auxin is a positive regulator for ethylene

mediated response in the growth of Arabidopsis roots. Plant Cell Physiol. 42:301-307 10. Rahman A, Tsurumi S, Amakawa T, Soga K, Hoson T, Goto N, Kamisaka S (2000) Involvement

of ehylene and gibberellin signalings in Chromosaponin I-induced cell division and cell elongation in Arabidopsis seedlings. Plant Cell Physiol. 41: 1-

5. Patents (PCT applications/granted) Patent : SMAP1, a novel gene in regulating the 2,4-D action in dicot plants (pending) 6. Competitive Research Grants obtained 3,000$- “Special faculty award” from Dean of Agriculture faculty, Iwate University 50,000$ for the Fiscal year 2007-2008 from MEXT, Japan 7. Size and qualifications of research team supervised Currently, I am supervising two Ph.D students in Iwate University, Japan. Previously I supervised

senior year research of 4 undergraduates in UMASS, Amherst, USA 8. National and International Collaborations International Collaboration:

Dr. Tobias Baskin, University of Massachusetts, Amherst, USA Dr. Gloria Muday, Wake Forest University, North Carolina, USA Dr. Elison Blancaflor, Nobel Foundation, Ardmore, Oklahoma, USA Dr. Seiji Tsurumi, Kobe University, Kobe, Japan Dr. Yutaka Oono, Japan Atomic Energy Research Institute, Takasaki, Japan Dr. Malcolm Bennett, University of Nottingham, Nottingham, UK

National Collaboration: No collaboration with Bangladesh yet. 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?)

153

Not applicable C. Capacity Development and Training Activities in Bangladesh:

(Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Yes. I am one of the founder members of GNOBB (Global network for Bangladeshi

Biotechnologists), which is a web based forum for Bangladeshi scientists all over the world. We are trying to make a bridge between the local and expatriate scientists to bring new ideas and collaboration for developing an internationally competitive science infrastructure in Bangladesh. This forum is also dedicated to create awareness among the scientists on different issues including the national biotech policy.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Understanding the hormonal response in growth and development of plant is a basic issue in

enhancing the crop yield and quality. Hence, my research can contribute potentially in these areas. For example, we are now dealing with a mutation which made the Arabidopsis plant extremely dwarf. The identification of this gene will contribute potentially to understand the regulatory mechanism of plant height, which then can be used to regulate the height of the crops in different environmental conditions. In addition, we have developed a project related to arsenic, which is a national problem of Bangladesh. We are also planning to initiate a project related to the development of plant based vaccine in near future. Taken together, I think the on going research projects in my lab reflect how Bangladesh can be benefited form my research and my expertise.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? With pleasure. However, the students or the young researchers have to secure the funding by

themselves. 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Definitely. A two week long course either in workshop format or teaching format will be suitable for

me. Again, the funding for such courses has to be arranged by the local host. 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? I can not do it right now as I have joined in a new position with certain commitments. However, I will

be interested to go back to Bangladesh in future. A well equipped research lab, good amount of startup funding (at least for five years) and a moderate salary will encourage me to think to relocate to Bangladesh.

6. Any other comments? I am very glad to see the momentum that is built by the Bangladeshi scientists in formulating the

National Biotech policy. I hope this workshop would immensely help us to understand the importance of the biotechnology and help to create a very progressive policy. However, I am surely disappointed to see so many sub committees and members for arranging this conference. Unfortunately, even without any contribution from my part, my name was put in some sort of sub committees. I wish we will be able to break this bureaucratic formulation of putting everyone’s name for no reason and do something really positive for the betterment of our country. My best wishes for a successful meeting!

154

Ashfaque Hossain A. Biosketch of Participants:

1. Name with academic title Ashfaque Hossain, Ph.D. 2. Current position with affiliation, address and contact details Senior scientist, Molecular biology, Creighton University School of Medicine 2500 California Plaza, Omaha, NE 68178, USA Phone (402) 280-2389 E.mail: [email protected] 3. Immediate past position(s) a. Research Scientist, Avant Immunotherapeutics, Boston, MA 4. Graduate and postgraduate degrees and training B.Sc. (Biochemistry), University of Dhaka, Bangladesh M.Sc. (Microbiology), University of Dhaka, Bangladesh Ph.D. (Microbiology), University of Glasgow, U.K. Postdoctorate (Virology), University of Nebraska, Lincoln, NE, USA Postdoctorate (Cell Biology), Harvard Medical School, Boston, MA USA 5. Prizes/Awards/Honours (academic and research)

Commonwealth Scholarship, 1985-1988. From Commonwealth Scholarship Commission, U.K. to do Ph.D. research at the University of Glasgow, U.K.

6. Elected Fellowships of Academies 7. Research Advisory Positions/Journal Editorship/Directorship of Boards 8. Membership of Institutional Committees B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research.

a. Studies on immunodeficiency in humans using a variety of immunology, cell biology and molecular biology techniques.

b. Interaction of Salmonella typhimurium with mammalian cells. 2. Area(s) of Expertise

Microbiology, Cell and Molecular biology, Virology, Immunology 3. Number of peer-reviewed publications

Thirty five 4. List of 5-10 most significant publications

Hanson, N.D., Hossain, A. Buck, A. Moland, E.S. and Thomson, K.S. (2006). The First Occurrence of a Pseudomonas aeruginosa in the United States Producing an IMP Metallo-β-lactamase, IMP-18. Antimicrobial Agents and Chemotherapy, 50, 1272-2273. Hossain,

A, Ferraro,M. J., Pino. R. M.3, Dew, R. B. III, Moland, E.S., Lockhart, T. J., Thomson, K. S. Goering, R. V. and Hanson, N.D. (2004) Plasmid-mediated Carbapenem hydrolyzing enzyme, KPC-2, in Enterobacter sp. Antimicrobial Agents and Chemotherapy, 48, 4438-4440. Hossain, A. Reisbig, M.D. and Hanson, N.D. (2004). Plasmid-encoded functions compensate for the biological cost of AmpC over-expression in a clinical isolate of Salmonella typhimurium. Journal of Antimicrobial Chemotherapy 53, 964-970.

Expatriates

155

Pitout, J.D.D., Hossain, A. and Hanson, N.D. (2004). Phenotypic and molecular detection of CTX-M-� lactamases produced by Escherichia coli and Klebsiella spp. Journal of Clinical Microbiology, 42, 5715-5721. Jiang, Y., Hossain, A., Winkler, M. T., Holt, T., Doster, A. and Jones, C. (1998). A protein encoded by the latency-related gene of bovine herpes virus 1 is expressed in trigeminal ganglionic neuron of latently infected cattle and interacts with cyclin dependent kinase 2 during productive infection. Journal of Virology, 72, 8133-8142. Hossain, A., Holt, T. Zanella, J. and Jones, C. (1997). Analysis of cyclin dependent kinase activity after herpes simplex virus type 2 infection. Journal of Virology, 78, 3341-3346. Schang, L. M., Hossain, A. and Jones, C. (1996). The latency-related gene of bovine herpesvirus 1 encodes a product which inhibits cell cycle progression. Journal of Virology. 70:3807-3814 Hossain, A., Schang, L. and Jones, C. (1995). Identification of gene products synthesized from the latency related gene of bovine herpes virus 1. Journal of Virology. 69: 5345-5352. Hossain, A., Freer, J.H. and Stewart-Tull, D.E.S. (1993). Heat-labile and heat-stable toxins of Campylobacter jejuni. FEMS Medical Microbiology and Immunology. 6: 331-340.

5. Patents (PCT applications/granted) Developed a technology for RNA isolation- gene expression studies (patent pending). California based Biotechnology company Invitrogen has developed a kit “Trizol-Max” based upon this technology and the product is in the market since 2004.

6. Competitive Research Grants obtained Yes 7. Size and qualifications of research team supervised 3; Graduate students, technicians. 7. National and International Collaborations 8. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. No. 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? By serving as a consultant to Biotechnology Company / Research Institute 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Yes 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? Yes; suitable teaching / research position 6. Any other comments?

There should be a National biotechnology coordination committee that will coordinate biotechnology research in different laboratories in Bangladesh. It should be able to identify viable, marketable products / technology and serve as platform for taking these to the Biotechnology companies both in Bangladesh and abroad. It should serve as liaison body between foreign / local investors and the local Biotechnology research laboratories.

156

Azizul Haque, PhD

A. Biosketch of Participants: 1. Name with academic title: Azizul Haque, PhD 2. Current position with affiliation, address and contact details: Assistant Professor, Department of

Microbiology and Immunology; 173 Ashley Avenue, BSB-201, Medical University of South Carolina, SC 29425, USA (2004-present).

Associate Member, Hollings Cancer Center, MUSC, SC 29425, USA (2004-present). Graduate Faculty, College of Medicine, MUSC, Charleston (2004-present) 3. Immediate past position(s): Research Assistant Professor, Department of Microbiology &

Immunology, IUSM, Indianapolis (2003-2004); Associate Member, IU Cancer Center, Indianapolis, (2003-2004).

4. Graduate and postgraduate degrees and training: Predoctoral Fellow, Department of Microbiology, Saga Medical School, Japan (1993-1997); Postdoctoral Fellow, Department of Microbiology & Immunology, Indiana University School of Medicine (IUSM), Indianapolis, IN, USA (1997-2003).

5. Prizes/Awards/Honours (academic and research): Japanese Government Predoctoral Fellowship, Japan (1993-1997); Marquis’ Who is Who in the World (1998-present); NRSA (1999-2000); Postdoctoral Fellowship, Arthritis Foundation (2001-2002); Career Development Award, Leukemia and Lymphoma Society (2003-2006); HCC Award (2006); ACS-IRG Award (2005-2006).

6. Elected Fellowships of Academies: N/A 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Ad hoc Reviewer, MUSC

Research Committee (2005-present), Ad hoc reviewer, Circulation (2000-present); Ad hoc reviewer, Journal of Immunology (2001-present); Ad hoc reviewer, Dutch Cancer Society (2001-present); Ad hoc reviewer, Journal of Leukocyte Biology (2003-present); Ad hoc reviewer, MUSC Research Committee (2005-present); Ad hoc reviewer, Differentiation (2006-present); Ad hoc reviewer, Journal of Biological Chemistry (2006-present); Ad hoc reviewer, Cellular Immunology (2006-present); Mentor/co-mentor, graduate advisory committee (2005-present); Member, graduate search committee (2005-present).

8. Membership of Institutional Committees: Japanese Society for Immunology (1994-1997), Japanese Society for Microbiology (1993-1997), American Society for Cell Biology (1998-2000), American Association of Immunologists (1999-present), American Association for the Advancement of Science (2000-2004), Associate Member, Hematopoiesis Program, Indiana University Cancer Center (2003-2004); Member, Hematological Malignancies Focus Group, MUSC (2004-present); Member, Gene Medicine, Research Foci of Excellence, MUSC (2004-present); Associate Member, Hollings Cancer Center (2004-present); Neuro-oncology Focus Group, Hollings Cancer Center (2004-present); MUSC Graduate Faculty (2004-present); Member, Center for Cell, Gene and Vaccine Therapy (2005-present); Member, Tumor-Host Interactions Research Program at Hollings Cancer Center (2005-present).

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Current work focused on

immunobiology of lymphoma, melanoma and prostate cancer. 2. Area(s) of Expertise: Tumor Immunology, autoimmunity and transplantation immunlogy 3. Number of peer-reviewed publications: 31 4. List of 5-10 most significant publications:

Haque, M.A., M. Kimoto, S. Inada, O. Tokunaga, and O. Kohashi. 1998. Autoreactive CD4−CD8− αβ T cell to vaccinate adjuvant arthritis. Immunology 94:536-642. Haque, M.A., J.W. Hawes, and J.S. Blum. 2000. Cysteinylation of an MHC Class II Ligand:

Expatriates

157

importance endocytosis and reductive processing in T cell recognition. J. Immunol. 166:4543-4551. Haque, M.A., P. Li, S.K. Jackson, H.M. Zarour, J.W. Hawes, U.T. Phan, M. Maric, P. Cresswell, and J.S. Blum. 2002. Absence of gamma-interferon-inducible lysosomal thiol reductase in melanomas disrupts T cell recognition of select immunodominant epitopes. J. Exp. Med. 195:1267-1277. Haque, M.A., T/ Mizobuchi, K. Yashufuku, T. Fujisawa, R. Brutkiewicz, Y. Zheng, K. Woods, G.N. Smith, O.W. Cummings, K.M. Heidler et. al. 2002. Evidence for immune responses to a self-antigen in lung transplantation. J. Immunol. 169:1542-1549. Li, Ping, M.A. Haque, and J.S. Blum. 2002. Role of disulfide bonds in regulating antigen Processing and eiptiope selection. J. Immunol. 169:2444-2450. Mizobuchi, T., K. Yasufuku, Y. Zheng, M.A. Haque, K.M. Heidler et. al. 2003. Differential expression of Smad7 and TCR V- � transcripts identify the CD4-CD45ROhi regulatory T cells that mediate type V collagen-induced tolerance to lung allografts. J. Immunol. 171:1140-1147. O'Donnell P.W., A. Haque, M.J. Klemsz, M.H. Kaplan, J.S. Blum. 2004. Induction of the antigen processing enzyme IFN-gamma-inducible lysosomal thiol reductase in melanoma cells is STAT1-dependent but CIITA-independent. J. Immunol (Cutting Edge). 173:731-735. Srinivasan, M., L. Debao, E. Rajaraman, D. Brand, A. Haque and J. S. Blum. 2005. CD80 binding polyproline helical peptide inhibits T cell activation. J. Biol. Chem. 280:10149-55. Haque A, Blum JS. 2006. New insights in antigen processing and epitope selection: development of novel immunotherapeutic strategies for cancer, autoimmunity and infectious diseases. J. Biol. Regul. Homeost. Agents. (Review) (in press). Yoshida, S., A. Haque, T. Mizobuchi, T. Iwata. et al. 2006. Anti-type V collagen lymphocytes that express IL-17 and IL-23 induce rejection pathology in fresh and well- healed lung transplants. Am. J. Transplant. (in press).

5. Patents (PCT applications/granted): Immunotherapy of prostate cancer 6. Competitive Research Grants obtained: AFR, LLS, NIH, ALA, HCC and ACS 7. Size and qualifications of research team supervised: 7 members including the PI 8. National and International Collaborations: UK, Germany, Japan and US 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): N/A C. Capacity Development and Training Activities in Bangladesh:


please provide brief information. Yes, collaborative studies are planned with Dr. F. Qadri (MS thesis mentor)

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Dr. Haque has an established laboratory at MUSC with expertise in tumor immunology, autoimmunity and transplantation immunology. Currently, many graduate students, postdoctoral fellows, residents, research technicians are working in Dr. Haque’s laboratory which is well funded. Certainly, these resources can benefit biotechnology research anywhere in the world including Bangladesh.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? Yes

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? N/A at this point

6. Any other comments? Would like to help anyway

158

Manujendra Narayan Saha, Ph.D. A. Biosketch of Participants: 1. Name with academic title: Manujendra Narayan Saha, Ph.D. 2. Current position with affiliation, address and contact details Postdoctoral Research Fellow (Japanese Society for the Promotion of Sciences, JSPS) Dept. of Virology and Preventive Medicine Gunma University Graduate School of Medicine Showa machi 3-39-22, Maebashi City, Gunma 371-8511, Japan Tel. Fax. +81-27-231-2186 E-mail. [email protected] 3. Immediate past position(s) Research Associate Dept. of Virology and Preventive Medicine Gunma University Graduate School of Medicine Showa machi 3-39-22, Maebashi City, Gunma 371-8511, Japan 4. Graduate and postgraduate degrees and training B. Sc. (Hons), M. Sc. (Biochemistry) Dept. of Biochemistry, University of Dhaka, Dhaka, Bangladesh Ph. D. (Virology) Dept. of Virology and Preventive Medicine Gunma University Graduate School of Medicine Maebashi, Gunma, Japan 5. Prizes/Awards/Honours (academic and research) Doctoral scholarship from Association for International Education in Japan (AIEJ) 6. Elected Fellowships of Academies Japanese Society for the Promotion of Sciences (JSPS) postdoctoral fellowship 7. Research Advisory Positions/Journal Editorship/Directorship of Boards None 8. Membership of Institutional Committees Bangladesh Biochemical Society (BBS) Graduate Biochemist Association (GBA) Japanese Society of Virology

B. Research and Research Management Experience:

1. Major project area(s); with very brief description of current research Study on the early infection mechanism of hepatitis viruses mainly, HBV and HCV. Infectivity assays

have been done using vesicular stomatitis virus (VSV) pseudotypes bearing surface proteins of HBV and HCV. Because of the lack of suitable and reliable assay systems, study on the HBV infection has been impaired. Assay system based on the pseudotypes are rapid and safe and we have been using this newly developed system for the infectivity assay of HBV in vitro.


Expatriates

159

Virology, Immunology, Bacteriology, Molecular Genetics, Genetic Engineering, Vibrio cholerae, Human immunodeficiency virus-1 (HIV-1), Hepatitis B virus (HBV), Hepatitis C Virus (HCV).

3. Number of peer-reviewed publications: Five 4. List of 5-10 most significant publications

Manujendra N. Saha, Atsushi Tanaka, Atsuhi Jinno-Oue, Nobuaki Shimizu, Kazushi Tamura, Masahiko Shinagawa, Joe Chiba, and Hiroo Hoshino. Formation of vesicular stomatitis virus pseudotypes bearing surface antigens of hepatitis B virus. J. Virol. 2005. 79:12566-12574 Faruque, S. M., Manujendra N. Saha, Asadulghani, D. A. Sack, R. B. Sack, Y. Takeda, G. B. Nair. The O139 serogroup of Vibrio cholerae comprises diverse clones of epidemic and nonepidemic strains derived from multiple V. cholerae O1 or non-O1 progenitors. J Infect Dis. 2000. 182:1161-8 Faruque, S. M., Manujendra N. Saha, Asadulghani, P. K. Bag, R. K. Bhadra, S. K. Bhattacharya, R. B. Sack, Y. Takeda, G. B. Nair. Genomic diversity among Vibrio cholerae O139 strains isolated in Bangladesh and India between 1992 and 1998. FEMS Microbiol Lett. 2000. 184:279-84 Faruque, S. M., A. K. Siddique, Manujendra N. Saha, Asadulghani, M. M. Rahman, K. Zaman, M. J. Albert, D. A. Sack, R. B. Sack. Molecular characterization of a new ribotype of Vibrio cholerae O139 Bengal associated with an outbreak of cholera in Bangladesh. J Clin Microbiol. 1999. 37:1313-8 Faruque, S. M., Asadulghani, Manujendra N. Saha, A. R. Alim, M. J. Albert, K. M. Islam, J. J. Mekalanos. Analysis of clinical and environmental strains of nontoxigenic Vibrio cholerae for susceptibility to CTXPhi: molecular basis for origination of new strains with epidemic potential. Infect Immun. 1998. 66:5819-25

5. Patents (PCT applications/granted) Formation of vesicular stomatitis virus pseudotypes bearing surface antigens of hepatitis B virus (Japanese patent)

6. Competitive Research Grants obtained Two years research grant from Japanese Society for the Promotion of Sciences (JSPS) 7. Size and qualifications of research team supervised None 8. National and International Collaborations

Our laboratory has been in collaboration with Dept. of Microbiology, University of Dhaka, ICDDR. B, and Chiangmai University, Thailand.

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Our laboratory has been in collaboration with Dept. of Microbiology, University of Dhaka, and Virology Laboratory, ICDDR, B. We are particularly interested in basic research on the viral diseases which are prevalent in Bangladesh like HBV, HCV etc, and which are not prevalent right now but may be at risk in near future, like HIV.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? I think, research in Biotechnology is going to improve a lot by 21st century and all researchers in home and abroad will try their best to be involved in these projects. My research experience after graduation is very much related with the field of Biotechnology, especially in medical science. I would be happy if I could be of any help for the well-being of the people of Bangladesh as well as for the country through application of my research works.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Few years back, by the strong leadership of my supervisor we were able to sign an exchange program between Gunma University and University of Dhaka. Through this exchange program several students and teachers already visited our laboratory in a short term training program. We hope to continue this program for the next few years.

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? I would be interested in doing so but that would depend on several factors.

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return?

160

If I have been offered a good working environment I may consider returning in my home country. 6. Any other comments?

None.

Minhaz Uddin Ahmed A. Biosketch of Participants: 1. Name with academic title Minhaz Uddin Ahmed Doctoral Student 2. Current position with affiliation, address and contact details

Dna Biosensor Team Japan Adv. Institute Of Science And Technolgy (Jaist) Ishikawa, Japan EMAIL: [email protected] PHONE: +81-761-51-1668 FAX: +81-761-51-1665

3. Immediate past position(s) A. Teaching Assistant (Ta) (Dec 2006- Till To Date) School of Materials Science Japan Adv. Institute of Science And Technolgy (Jaist) Ishikawa, Japan B. Scientific Officer (Feb 2005- Sep 2005) National Forensic DNA Profiling Laboratory Department of Forensic Medicine Dhaka Medical College

5. Graduate and postgraduate degrees and training Ms: Biochem. And Molecular Biology (Du) 1999-2000 Bsc: Biochem. And Molecular Biology (Du) 2000-2001 6. Prizes/Awards/Honours (academic and research)

a. Centre of Excellence (COE) Award from Ministry of Education, Culture and Sports, Japan and JAIST, 15 November 2005

b. Japanese Government (Monbukagakusho;MEXT) Scholarship from Ministry of Education, Culture and Sports, Japan

c. Education Board Merit Scholarships etc B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research Development Of Dna Biosensor Chip 2. Area(s) of Expertise

Biochemistry, Biotechnolgy Applied Biotechnolgoy And Materials Science

3. Number of peer-reviewed publications Two (2) 4. List of 5-10 most significant publications

M. U. Ahmed and S. Akhteruzzaman. Apolipoprotein E gene Polymorphism in Bangladeshi population and its comparison with other asian populations, Journal of Medical Science, March-April, 2006 M. U. Ahmed, K. Idegami, M. Chikae, K. Kerman, P. Chaumpluk, S. Yamamura, and E. Tamiya. Electrochemical DNA biosensor using disposable electrochemical printed (DEP) chip for the

Expatriates

161

detection of SNPs from unpurified PCR amplicons, In Press, 2007, The Analyst, Royal Society of Chemistry (RSC) Journal, Cambridge, UK

5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained 7. Size and qualifications of research team supervised 8. National and International Collaborations

a. School of Science, Chulalongkorn University, Phyathai, Bangkok 10330, Thailand. b. Ishikawa Sunrise Industries Creation Organization, 2-20, Kuratsuki, Kanazawa City, Ishikawa 920-8203, Japan Biodevice Technology Co. Ltd. Japan

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh: (Information requested from

Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. No, But Seeking Collaborators To Establish The Dna Sensor Technology In Bangladesh

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? In Brief, Disease Diagnosis, Environmental Monitoring 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? 4. Would you be interested in conducting and/or participating in short-term teaching and training


conditions would induce you to return? I Would Consider To Return Bangladesh Either for Two Reason 1. To Start From The Beginning 2. Or, To Drive the Ongoing Continuning Efforts for Both Long and Short Terms 6. Any other comments?

Please, Tell Us in Detail a. The Purpose Of This Questionnaire b. The True Future Prospect of Biotech. Based Industry/Research In Bangladesh c. The Possibility To Establish Nanotechnology Research Centre In Bangladesh Finally, I Would Like To Thanks the Organizers of This Workshop, Especially Prof. Haseena Khan and Prof. Zeba I. Seraj.

162

Md. Monirul Islam

A. Biosketch of Participants: 1. Name with academic title:

Dr. Md. Monirul Islam 2. Current position with affiliation, address and contact details: Senior Molecular Biologist, Ballarat Cancer Research Centre, University of Ballarat, c/o- St. John of

God Hospital, 101 Drummond Street, Ballarat 3350, Australia. Phone: 61-3-5320 2404, Mob: 0401329275

3. Immediate past position(s): Research Associate, Department of Biological Science, Southern Methodist University, Dallas, Texas, USA.

4. Graduate and postgraduate degrees and training: Masters in Biochemistry, University of Dhaka, Bangladesh; Ph.D. in Biochemistry, department of biomedical chemistry, faculty of medicine, University of

Nagoya, Japan 5. Prizes/Awards/Honours (academic and research) : Travel fellowship award for attending the 16th AMBO International Training Course in Molecular

Biological and Biochemical Methods in Bioenergetics, 1993, Osaka, Japan granted by the AMBO Fellowship Committee.

Travel fellowship award for attending the 7th FAOBMB Congress, 1995, Sydney, Australia; granted by the FAOBMB Fellowship committee.

6. Elected Fellowships of Academies: Japanese Ministry of Education, Science and Culture (Monbusho) Scholarship carry out Ph.D program at the Department of Biomedical Chemistry, University of Nagoya, Japan,

Oct 1991 - Mar 1996. Japan Science and Technology Agency (STA) Fellowship, granted by the Japan International Science

and Technology Exchange Centre, Oct 2000-Sep 2002. Jack and Millie Borbidge Cancer Research Fellowship, granted by the University of Ballarat,

Australia, Jul 2003 – June 2005. 7. Research Advisory Positions/Journal Editorship/Directorship of Boards -None 8. Membership of Institutional Committees: Specialist molecular biologist, IBC (Institutional Bio-safety

committee), University of Ballarat, Australia. B. Research and Research Management Experience: 1. Major project area(s): Possible Retroviral Association in LCH Etiology. Langerhans Cell

Histocytosis (LCH) is characterized by an infiltration of antigen presenting Langerhans Cells in a wide variety of tissues (bone marrow, lungs, gut, liver, spleen, thymus, lymph nodes and brain) resulting in tissue damage or severe organ failure and death, especially in young children. The etiology of the disease is not yet known. Among many other suggestions viral association is considered as one of the main suggestion. I am trying to clone any suspected viral gene either directly from the LCH tissues or from the thymoma cell-line developed in LCH xenografted SCID mouse.

2. Area(s) of Expertise: Ageing, Cancer and Molecular Virology 3. Number of peer-reviewed publications: No mention 4. List of 5-10 most significant publications: No mention 5. Patents (PCT applications/granted): None 6. Competitive Research Grants obtained: None

Expatriates

163

7. Size and qualifications of research team supervised: Team four members, post-doctorate, Ph,D students and research assistants.

8. National and International Collaborations: None 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): Not

applicable C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information : No 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Not

sure 3. Can you provide research training to Bangladeshi students and young researchers in your

laboratory?No 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Not sure. Possibly yes. 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? - Yes. Social safety in terms of economy and livelihood are the two main conditions need to be changed.

6. Any other comments? : Very good initiative. Well done and good luck.

164

Md. Rafiqul Islam A. Biosketch of Participants: 1. Name with academic title Md. Rafiqul Islam, M. Sc.(DU), M. Phil. (DU) 2. Current position with affiliation, address and contact details Ph.D. fellow, Graduate School of Life Science, University of Hyogo 3-2-1, Koto, Kamigori, Ako, Hyogo 678-1297, Japan E-mail- [email protected], Tel. 81-90-6540-2921 3. Immediate past position

Assistant Professor (on study leave) Department of Biotechnology and Genetic Engineering Islamic University, Kushtia, Bangladesh

4. Graduate and postgraduate degrees and training M. Sc.: Department of Biochemistry and Molecular Biology, University of Dhaka M. S.: Department of Life Science, University of Hyogo, Japan M. Phil.: Department of Biochemistry and Molecular Biology, University of Dhaka

5. Prizes/Awards/Honours (academic and research) A-grade scholarship given by Dhaka University for good result during B. Sc. (honours) NST fellowship, during M.Sc., given by the Ministry of Science and technology, Bangladesh NST fellowship, during M. Phil., given by the Ministry of Science and Technology, Bangladesh Monbusho scholarship, during MS and Ph.D. program, given by the Ministry of Science and Technology, Japan

6. Elected Fellowships of Academies : NA 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: NA 8. Membership of Institutional Committees: NA B. Research and Research Management Experience: 1. Major project area (s); with very brief description of current research

Rice and Jute plant transformation Molecular characterization of coastal rice for salt tolerant trait Current research: Trying to elucidate the function of the gene slr1923 of Synechocystis 6803 by inactivation through site-directed mutagenesis. A great advance has already achieved. Through biochemical and molecular characterization of the mutant and upon comparison with wild type strain, it has proved that the targeted gene (slr1923) encodes 3, 8-divinylchlorophilide a 8-vinyl reductase which is responsible for the vinyl group (linked to the B ring) reduction of premature chlorophyll molecule. Therefore mutant accumulate DV (divinyl) chlorophyll a instead of MV (monovinyl)

Expatriates

165

chlorophyll a. Structural alteration of the chlorophyll a molecule caused several kind of reduced photosynthetic activities.

2. Area(s) of Expertise Molecular Biology, Plant Biotechnology 3. Number of peer-reviewed publications: 4 (Four) 4. List of 5-10 most significant publications

Zeba I. Seraj, Laisa A. Lisa, M. Rafiqul Islam, Rokeya Begum and Deepok K. Das (2005) Genetic diversity of saline coastal rice (Oryza sativa L.) landraces of Bangladesh. Abiotic Stress tolerance in Plants, Springer The Netherlands, Ashwani K. Rai and Teruhiro Takabe (Eds.), 2005:229-244. Laisa Ahmed Lisa, Zeba I. Seraj, C.M. Fazle Elahi, Keshob C. Das, Kuntal Biswas, M. Rafiqul Islam, M. Abdus Salam and A.R. Gomosta (2004) Genetic variation in microsatellite DNA, physiology and morphology of coastal saline rice (Oryza sativa L.) landraces of Bangladesh. Plant and Soil 263(1-2): 213-228. M. Rafiqul Islam, Mahboob Hossain Khan, Fatema T. Zohra, M. Bakhtiar Hossain and Zeba I. Seraj (1999) Stable transformation of Jute (Corchorus capsularis L. var CVL-1) calli and high efficiency maker gene insertion in Explants. Plant Tissue Culture 9(1):35-43. Noorain M. Rasul, K. Mohammad Ali, M. Rafiqul Islam and Zeba I. Seraj (1997) Transformation of an indica trice cultivar binnatoa with Agrobacterium tumefaciens. Plant Tissue Culture 7(2): 71-80.

5. Patents (PCT applications/granted): NA 6. Competitive Research Grants obtained: NA 7. Size and qualifications of research team supervised: NA 8. Natinal and International Collaborations: NA 9. Major Equipments/Facilities in Bangladesh (will these be available to other researchers): NA

Additional Proposal: • Need to pursue very strongly to the BD government to consider biotechnology as a thirst sector. So that they can allocate some special fund in the next budget. • At least one or two international grade lab. can be set up in those universities where there is a department of biotechnology. • A motivation scheme can be taken for the private businessman (specially pharmaceuticals) so that they can give some research funding to the universities. • A world class research can be carried out in the national institute of biotechnology (NIB) of BD under valuable supervision of BD scientist staying in home and in abroad.

166

Subarna A. Khan, Ph.D. A. Biosketch of Participants: 1. Name with academic title: Subarna A. Khan, Ph.D. 2. Current position with affiliation: Development Scientist Bioanalytical Sciences Imclone Systems Incorporated Address and contact details: 3001 Merrywood Drive Edison, NJ-08817 732-404-7733 (Cell) [email protected] 3. Immediate past position(s): N/A

Graduate and postgraduate degrees and training: Ph.D., Microbiology & Molecular Genetics M.S., Microbiology & Molecular Genetics Rutgers, The State University of NJ 4. Prizes/Awards/Honours (academic and research): 3rd Position, B.Sc. (Hons.) (1st Batch) Microbiology University of Dhaka B.S., Microbiology, 1995 Summa Cum Laude Outstanding Student Honoree Oregon State University Corvallis, OR, USA. 5. Elected Fellowships of Academies: N/A 6. Research Advisory Positions/Journal Editorship/Directorship of Boards: N/A 7. Membership of Institutional Committees: N/A B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Doctoral Thesis Title:Regulation of CD44 and apoptosis-related gene Bcl-XL expression by

osteopontin (OPN) Project one: By utilizing various molecular biology techniques investigated affect of osteopontin (OPN), a protein

involved in cancer metastasis, on expression, incorporation, and abundance of cell surface receptor CD44 variant exons in specific CD44 isoform mRNA in osteopontin-transfected and vector-transfected human breast cancer cells (21NT). Also evaluated cell surface and total protein expression of different CD44 variants as a result of endogenous overexpression and exogenous addition of OPN. Project two:

Expatriates

167

Induced growth factor deprived apoptosis in human umbilical vein endothelial cells (HUVECs) and investigated affect of soluble OPN on the abundance, distribution of anti-apoptotic protein Bcl-XL and its mRNA expression by different molecular biology techniques.

M.S. Thesis Title: Evidence that the mouse rough coat gene (rc) is a mutant allele of the lysyl oxidase-like gene (Loxl) Project:

Generated sterile mouse colonies by breeding BALB/c and C57BL/6-rc/rc parental strains. Generated linkage maps of the proximal region of the Loxl gene on mouse chromosome 9 and mapped the rc locus by investigated DNA polymorphism on the basis of linkage analysis using DNA microsatellite marker polymorphism, intron length polymorphism (ILP), and restriction fragment length polymorphism (RFLP).

2. Area(s) of Expertise: Cell biology, Molecular Biology, Immunology, Assay Method Development in the industry under

current good manufacturing practice (cGMP) regulations 3. Number of peer-reviewed publications: 4 4. List of 5-10 most significant publications: N/A 5. Patents (PCT applications/granted): N/A 6. Competitive Research Grants obtained: N/A 7. Size and qualifications of research team supervised: N/A 8. National and International Collaborations: N/A 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): N/A

168

Dr. Abul Faiz Md. Jamal Uddin

A. Biosketch of Participants: 1. Name with academic title: Dr. Abul Faiz Md. Jamal Uddin 2. Current position with affiliation, address and contact details

Assistant Professor Department of Horticulture and Postharvest Technology Sher-e-Bangla Agricultural University Sher-e-Bangla Nagar, Dhaka 1207 and Post Doctoral Research Fellow River Basin Research Center Gifu University, Gifu 501-1193, Japan Tel: +81-58-293-2063

3. Immediate past position(s) 4. Graduate and postgraduate degrees and training

2000-2003 (3 years) Degree: Doctor of Philosophy in Agricultural Science (Ph.D) Institution: The United Graduate School of Agricultural Science, Kagoshima, Japan Degree awarded on 14 March 2003 1998- 2000 (2 years) Degree obtained: Masters of Science (Horticulture) Institution: Kagoshima University, Kagoshima, Japan 1987-1989 (2 years) Degree obtained: Masters of Science (Agriculture) in Horticulture Institution: Bangladesh Agricultural University, Mymensingh, Bangladesh 1983-1987 (4 years) Degree obtained: Bachelor of Science in Agriculture (Honors) Institution: Bangladesh Agricultural University, Mymensingh, Bangladesh

Attended trainings • “Horticultural Nursery Development Technology”, April 7 - May 6, 1993, RDA, Bogra.

Bangladesh • “Agro forestry Research and Development “ 6-15 July, 1996, BARI, Joydebpur, Bangladesh • Wood and Fuel production and marketing in Forest, Agriculture and Tree production system in

Bangladesh, 7-11 December, 1996. Department of Forest, RDA, Bogra, Bangladesh • TOT, Training of Trainers, 16-27 July 1997. Kurigram Training Centre, Kurigram, RDRS.

Bangladesh 5. Prizes/Awards/Honours (academic and research) Academic and Research awards

Best Student Poster Presentation Award XXVIth International Horticultural Congress Symposia: Elegant Science in Floriculture, Toronto, Canada, August 11-17, 2002 Poster title: ‘Inheritance Model of Three Major Anthocyanidins in Eustoma grandiflorum Cultivars’ Best Poster Award

Local scientists

169

Doctoral Course Research Presentation at the general seminar, United Graduate School of Agricultural Science, Kagoshima University, Japan, November 12-14, 2002 Best Paper of the Year’ 2004 Award

Multiple allelism in flavonoid hydroxylation in Eustoma grandiflorum (Raf.) shinn. flowers. Journal of Japanese Society for Horticultural Science. 73: 235-240 7. Elected Fellowships of Academies

“Kohnan Asia Scholarship, Japan” - for pursuing the degree of ‘MS in Horticulture’, 1998-1999. Yoneyama Scholarship, Naka Somuta, Kagoshima shi-890, for pursuing the degree of ‘MS leading Ph.D, 1998-2003. “Honors Scholarship for Privately Financed International Students, Ministry of Education, Culture, Sports, Science and Technology, Japan for pursuing the doctoral course, 2002-2003. COE fund for Postdoctoral Research, River Basin Research Center, April 2006-March 2008

8. Research Advisory Positions/Journal Editorship/Directorship of Boards: Supervising the MS students of Sher-e-Bangla Agricultural University, Dhaka

9. Membership of Institutional Committees: Not applicable B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research Sequencing of rDNA: Development of data base of Japanese isolates of Phytophthora species

Phytophthora diseases are very important in ornamental horticulture (vegetable crops and trees as well), but the taxonomy of Phytophthora species is confusing. Phytophthora diseases are very important in ornamental horticulture (in vegetable crops as well), but the taxonomy of Phytophthora species is not clear. There are many miss-identified isolates, suggesting that the well-known type cultures and easy and precise identification procedure are required. Objectives of the present research are to re-identify the isolates based on morphological characteristics and molecular phylogeny and to develop the data base of neo-type cultures that will be certified on the present study. • Phylogenetic analyses of Phytophthora species based on the sequences of the ITS region of

rDNA, beta-tubulin gene and cytochrome oxidase II gene. • Development of date base of the sequences • Development of data base of the morphological characteristics • Development of rapid and precise identification procedure using PCR • Development of the web site to use the data base and the identification protocols

2. Area(s) of Expertise: Horticulture, Crop Pathology and Molecular Biology 3. Number of peer-reviewed publications: 12 4. List of 5-10 most significant publications

Uddin, A.F.M. Jamal, Hashimoto F., Kaketani M., Shimizu K., and Y. Sakata. 2001. Analysis of light and sucrose potencies on petal coloration and pigmentation of lisianthus cultivars (in vitro). Scientia horticulturae 89(1): 73-82. Uddin, A.F.M. Jamal, Hashimoto F., Nishimoto S., Shimizu K., and Y. Sakata. 2002. Flower growth, coloration and pigmentation of four lisianthus cultivars. J. Japan. Soc. Hort. Sci. 71(1): 40-47 Uddin, A.F.M. Jamal, Hashimoto F., Miwa, T., Ohbo, K., and Y. Sakata.2004. Seasonal variation in pigmentation and anthocyanidin phenetics in commercial Eustoma flowers. Scientia horticulturae 100:103-115 Uddin, A.F.M. Jamal, Hashimoto F and Y. Sakata. 2004. Monosaccharides and Chitosan sensing in bud growth and petal pigmentation in Eustoma grandiflorum (Raf.) shinn. Scientia horticulturae 100:127-138 Uddin, A.F.M. Jamal, Hashimoto F., and Y. Sakata. 2003. Mode of Inheritance and Allele Expression of three major anthocyanidins in Eustoma grandiflorum Cultivars. Acta Hortculturae 624: 51-59

170

F. Hashimoto, Jamal Uddin A.F.M, Shimizu K, and Y. Sakata. 2004. Multiple allelism in flavonoid hydroxylation in Eustoma grandiflorum (Raf.) shinn. flowers. J. Japan. Soc. Hort. Sci. 73: 235-240 T. Mustarin, K. Khatun, H. Kabir, A.F.M. Jamal Uddin and S.M.A. Zaman. 2005. Optimizing the productivity of French bean by appropriate management of nitrogen and mulch practices. International Journal of Sustainable Agricultural Technology (IJSAT). 1: 20-25 M.H. Kabir, M.R. Amin, T. Mostarin, K. Khatun, and A.F.M. Jamal Uddin. 2005. Effect of Pinching on growth and Yield of Broccoli (Brassica oleracea Var. Italica) International Journal of Sustainable Agricultural Technology (IJSAT).1 (3): 57-60 H. M Abdullah, A. S. M. Nahiyan and A.F.M. Jamal Uddin. 2005. Fundamental studies on Chemical composition and Shelf Life of Jackfruit and Jackfruit Products. J. Subtrop. Agricultural Research and Development (JSARD). 3(2): 12-19 H. M Abdullah, A. S. M. Nahiyan and A.F.M. Jamal Uddin. 2005. Quality seedling Production of Sweet Orange from True Seed (In vitro). International Journal of Sustainable Agricultural Technology 1(5): 21-23

5. Patents (PCT applications/granted): not applicable 6. Competitive Research Grants obtained: Not applicable 7. Size and qualifications of research team supervised: Not applicable 8. National and International Collaborations: Not applicable 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) Not

applicable C. Capacity Development and Training Activities in Bangladesh:


please provide brief information. Not applicable 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? I will teach my student 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes, I will try to establish a molecular biology lab in Bangladesh and there from the young scientist

will learn the modern molecular technique to improve the Agriculture 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Surly, I am interested. 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? Surely I will return to Bangladesh on April,2008 6. Any other comments? No comments

171

Dr. Md. Arifuzzaman A. Biosketch of Participants: 1. Name with academic title: Dr. Md. Arifuzzaman 2. Current position with affiliation, address and contact details Associate Professor Department of Biochemistry and Biotechnology University of Science and Technology, Chittagong (USTC) Foy’s Lake, Chittagong, Bangladesh. E mail: [email protected] 3. Immediate past position(s): Assistant Professor 4. Graduate and postgraduate degrees and training Bachelor of Medicine and Surgery (MBBS) MSc (Biological Science, Japan), PhD Training in Radioisotopes 5. Prizes/Awards/Honours (academic and research): a) “Most interesting paper award” Faculty of 1000 Biology (United Kingdon), October 2006 b) Japan Govt. Scholarship (Monbusho) January 1996- March 2002. 6. Elected Fellowships of Academies: JBA & NEDO Postdoctoral Fellowship, Japan: May 2002 – March 2004. 7. Research Advisory Positions/Journal Editorship/Directorship of Boards 8. Membership of Institutional Committees: Member HUPO (Human Proteomics Organization), Canada Member Society of Japan Molecular Biology B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Article I. Transcriptional regulation of stress proteins, Proteomics 2. Area(s) of Expertise: Article II. Cell biology, Genetic engineering, Proteomics 3. Number of peer-reviewed publications: Seven (7) 4. List of 5-10 most significant publications 1) Mohammad Arifuzzaman, Maki Maeda, Aya Itoh, Kensaku Nishikata, Chiharu Takita, Rintarou

Saito, Takeshi Ara, Kenji Nakahigashi, Hsuan-cheng Huang, Aki Hirai, Kohei Tsuzuki, Seira Nakamura, Mohammad Altaf-Ul-Amin, Taku Oshima, Tomoya Baba, Natsuko Yamamoto, Tomoyo Kawamura, Tomoko Ioka-Nakamichi, Masanari Kitagawa, Masaru Tomita, Shigehiko Kanaya, Chieko Wada, Hirotada Mori. “Large-Scale identification of protein-protein interaction of Escherichia Coli K-12.” Genome research (2006) 16 (5) 686-691.

2) Masanari Kitagawa, Takeshi Ara, *Mohammad Arifuzzaman, Tomoko Ioka-Nakamichi, Eiji Inamoto, Hiromi Toyonaga and Hirotada Mori “Complete set of ORF clones of Eschericia coli ASKA

Local scientists

172

library (A Complete Set of E.Coli K-12 ORF Archive): Unique resources for Biological Research.” DNA Research (2005) 12, 291-299.

3) Balan Venkatesh, *Md. Arifuzzaman, Hirotada Mori, S. Suzuki, Takashisa Taguchi and Yoshihiro Ohmiya. “Use of GFP tags to monitor localization of different luciferase in E.coli.” Photochem Photobiol. Sci. (2005) 4 (9), 740 – 3.

4) *Mohammad Arifuzzaman, Taku Oshima, and Hirotada Mori. “The ATPase domain of HscC (DnaK homolog) is essential for interfering �70 activity in E. coli.” FEMS Microbiology letters (2004) 230:1, 99-104.

5) *Mohammad Arifuzzaman, Taku Oshima, Shinsuke Nakade, and Hirotada Mori. “Characterization of HscC (Hsc62), homolog of Hsp70 in Escheriachia coli: overexpression of HscC modulates the activity of house keeping sigma factor �70.” Genes to cells (2002) 7, 553-566.

5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained 7. Size and qualifications of research team supervised Supervised PhD student in Japan (3) 8. National and International Collaborations International: Collaboration with Japan National: Vatinery University 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Protein-protein interactions should provide the foundations to facilitate elucidation of cellular

activities, targeted drug design and cell engineering. 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory?

Yes 4. Would you be interested in conducting and/or participating in short-term teaching and training


conditions would induce you to return? 6. Any other comments?

173

Farhana Afroz A. Biosketch of Participants: 1. Name with academic title: Farhana Afroz (B.Sc in BTGE, MS in Biotechnology) 2. Current position with affiliation, address and contact details: Scientific Officer (Genetic Engr. & Biotech.), Applied Botany Section, Biological Research Division,

BCSIR Laboratories, Dhaka. Mob: +8801716363506 3. Immediate past position(s): MS Student (Biotechnology, BAU) 4. Graduate and Post Graduate degrees and Training: Graduate: - BSc. in Biotechnology and Genetic Engineering, Khulna University. Post Graduate: - MS in Biotechnology, BAU. 5. Prizes/Awards/Honors (academic and research) : N/A 6. Elected fellowships of Academies: N/A 7. Research advisory positions/Journal editorship/Directorship of Boards: N/A 8. Membership of institutional committees: Member of Scientists Association, BCSIR. B. Research and Research Management Experience: N/A C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) N/A

Local scientists

174

Gautam Kumar Deb

A. Biosketch of Participants:

1. Name with academic title:

Gautam Kumar Deb, B.Sc in Animal Husbandry, M.S in Animal Breeding and Genetics.

2. Current position with affiliation, address and contact details: Article III. Scientific Officer

Animal Production Research Division Bangladesh Livestock Research Institute, Savar, Dhaka-1341. Phone: +88-02-7708321, Ext. 278 (off.) Fax No.: +88-02-7708325 Mobile: +88-01716-523-423 or +88-0191-256-364 Email: [email protected] or [email protected]

3. Immediate past position(s) Section 3.01 Scientific Officer Research on Characterization, Conservation and Improvement of Red Chittagong Cattle of Bangladesh” a USDA funded project. Department of Animal Breeding & Genetics, Bangladesh Agricultural University, Mymensingh-2202


Article IV. University Year Degree Class

Bangladesh Agricultural University, Mymensingh

2004 Master of Science in Animal Breeding and Genetics

First


2000 Bachelor of Science in Animal Husbandry

First

a. Training Acquired Name Organization/Institute Duration

Training Workshop on Acquisition & Management of Agro-forestry Knowledge

Bangladesh Livestock Research Institute Savar, Dhaka

23 -27 April 2006

ILRI-SLU South Asia Training Course on Capacity Building for Sustainable Use of Animal Genetic Resources

University of Peradeniya, Sri Lanka

13 February to 3 March 2006

Extension education training Bangladesh Agricultural University Mymensingh

9-14 February 2002

5. Prizes/Awards/Honors (academic and research): Not applicable

Local scientists

175

6. Elected Fellowships of Academies: Not applicable

7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Not applicable

8. Membership of Institutional Committees: Not applicable

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research:

Conservation and genetic improvement programme of cattle and buffalo of Bangladesh Livestock Research Institute. My present researches include molecular characterization of cattle and buffalo germplasms.

2. Area(s) of Expertise: Cattle breeding, molecular genetics and reproduction biotechnology

3. Number of peer-reviewed publications: 06

4. List of 5-10 most significant publications:

M. M. Hossain, A. K. F. H. Bhuiyan, M. O. Faruque & G. K. Deb, 2006. Characterization and distribution pattern of Red Chittagong cattle of Bangladesh. Progressive Agriculture, 17 (1): 103-110. M. M. Uddin, S. Ahmed, M. S. R. Siddiki, M. N. Islam & G. K. Deb. 2006. The stages of lactation genotypic influence on milk yield, lactation yield, lactation length and birth weight of calves. Bangladesh journal of Progressive Science and Technology, 4(1):89-92. A. K. F. H. Bhuiyan, M. S. A. Bhuiyan & G. K. Deb, 2005. Indigenous Chicken Genetic Resources of Bangladesh - Current Status and Future Outlook. Animal Genetic Resources Information, FAO-UNEP, Rome, Italy. G. K. Deb, M. M. Hussain & A. K. F. H. Bhuiyan, 2005. Estimation of Genetic Parameters for Economic Traits in Dairy cattle. Bangladesh Journal of Animal Science, 34(1 & 2): 17-26. G. K. Deb & M. A. I. Talukder, 2006. Genetic Evaluation of Native cattle for Birth Weight. Bangladesh Journal of Livestock Research, Savar, Dhaka (accepted). A. K. F. H. Bhuiyan, MM Hussain, G. K. Deb & M. S. A. Bhuiyan, 2006. Indigenous Cattle Genetic Resources of Bangladesh - an overview. Submitted to Animal Genetic Resources Information, FAO-UNEP, Rome, Italy.

5. Patents (PCT applications/granted): Not applicable

6. Competitive Research Grants obtained: Not applicable

7. Size and qualifications of research team supervised: Not applicable

8. National and International Collaborations: Not applicable

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): DNA extraction, synthesis and sequencing facility. These may be available for other researchers through institutional agreement

176

Gazi Nurun Nahar Sultana

A. Biosketch of Participants: 1. Name with academic title: Gazi Nurun Nahar Sultana, Ph. D. 2. Current position with affiliation, address and contact details: Senior Scientist, Centre of Excellence,

Dhaka University, Dhaka-1000, Tel: 9661900-59/4628/4630, 2006-until now. 3. Immediate past position(s): Post Doctoral Research Associate, University of Mississippi, USA, 2000-

2005 4. Graduate and postgraduate degrees and training: 1 year training on cloning research, RIKEN GENE

BANK, Tsukuba, Japan, 1997. 5. Prizes/Awards/Honours (academic and research): Awarded for training on “Basic Sequencing and Fragment Analysis”, ABI, UK. Awarded Project Grant from “Biotechnology Research Centre”, University of Dhaka, 2006-2007. Awarded Project grant for the development of research laboratory from the Ministry of Science and

Technology, Govt of Bangladesh. 1998-2000 6. Elected Fellowships of Academies: Monbusho fellowship for Ph. D. 1992-1995, Japan, AIST

fellowship for Training, 1996, Japan, STA fellowship as invited researcher, 1997, Japan, 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Consultant of CIDA for 6

months, 1999, Khamarbari, Dhaka, BD. Participated as Trainer for NITUB, Bangladesh. 8. Membership of Institutional Committees: American Chemical Society. (ACS) Japan Society for Bioscience, Biotechnology and Agrochemistry (JSBA) Bangladesh Biochemical Society (BBS) Bangladesh Association for the Advancement of Science (BAAS) Bangladesh Scientist Association (BSA). B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research Presently, I an engaged as a senior scientist in the Centre of Excellence, University of Dhaka. I am in-

charge of DNA Sequencing Laboratory and Atomic Absorption Spectrometry laboratory. I am involved in analytical method development, optimization and validation of sample preparation for DNA sequencing. My present research interest in Human DNA profiling as well Clinical Biochemistry. I am assigned to supervise MS, M.Phil and Ph.D. students in the field of Clinical Biochemistry.

2. Area(s) of Expertise: Clinical Biochemistry 3. Number of peer-reviewed publications: 16

Local scientists

177

4. List of 5-10 most significant publications Optimization of the Sample Preparation Method for DNA Sequencing, Gazi NN Sultana, Amir H. Khan, Asian J. Bio Sci. 7(1), 2007, 194-199. “Flavonoid Glycosides and Cannabinoids from the Pollen of Cannabis sativa L.” Ross S. A., ElSohly

M., Gazi NN Sultana, Mehmedic Z., Hossain, C. F., Chandra S., Phytochemical Analysis 2005, 16, 45-48.

"Syncarpamide, a new Antiplasmodial (+)-Norepinephrine Derivative from Zanthoxylum syncarpum Tul." Ross S. A., Gazi NN. Sultana; Burandt C. L., ElSohly M. A., Marais J. P. J., and Ferreira D.; J. Nat. Prod. 2004, 67, 88-90.

"Secondary Metabolites from Plants and Marine Organisms as Selective Anti-cyanobacterial Agents" in "Off-flavor in Aquaculture" (a book published by American Chemical Society as Symposium Series on May 2003 (Vol. 848) ISBN# 0-8412-3821-9), Chapter 13, page 179 ~ 194, Editors: Rimando A.M.; Schrader K.K.; Authors- Nagle, D.G.; Gazi NN. Sultana, Schrader, K.K., Hossain C.F., Stanikunaite, R., Hamann, M.T., Rajbandari, I., "Effect of Inorganic Phosphorus Compounds on Hydrolysis of Phosphatidylcholine Liposome by Phospholipid Deacylating enzyme" Gazi NN. Sultana, Watanabe Y., Tamai Y., J. Biotechnol. Appl. Biochem, 1995, 21, 101-110. "Cloning and Sequencing of Phospholipase B Gene from the Yeast Torulaspora delbrueckii" Watenabe Y., Yukinari Y., Gazi NN. Sultana, Kanagawa K and Tamai Y., FEMS Microbial Let., 1994, 124, 29-34. "Calcium Ion Dependent Interaction of Inorganic Phosphorus Compounds with Phosphatidylcholine" Gazi NN. Sultana, Watanabe Y., and Tamai Y., J. Biochem., 1993, 114, 251-254. "Inhibitory Effect of Glycerophosphorylcholine (GPC) on Phospholipase Enzymes" Gazi NN. Sultana, Watenabe Y., Tamai Y., Bangladesh Journal of Physiology and Pharmacology, 1997, 13(2), 52-54. Two New Alkaloids from Zanthoxylum syncarpum Tul.", Gazi NN Sultana, Ross S. A., Burandt C. L., (In press).

5. Patents (PCT applications/granted): N/A 6. Competitive Research Grants obtained: N/A 7. Size and qualifications of research team supervised: 4 MS Thesis students supervised in USA during

post doc. Presently supervising 2 MS students and one Ph.D Student. 8. National and International Collaborations: N/A 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): DNA

Sequencer, Atomic Absorption Spectrophotometer, UV Spectrophotometer, Nano pure water system. Yes, these facilities are available provided conditions.

C. Capacity Development and Training Activities in Bangladesh:

(Information requested from Expatriate Scientists)

1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so, please provide brief information.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? I can be involved for guiding post graduate students, offering training for instrumentations, support

for idea development regarding research grants, collaboration research can be offered with other Institutions through my active participation.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? Yes.

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return?


178

Md. Harun-or Rashid A. Biosketch of Participants: 1. Name with academic title: Md: Harun-or Rashid, B.Sc.Ag, M.S in Soil Science. 2. Current position with affiliation, address and contact details: Scientific officer, Soil Science Division,

Bangladesh Institute of Nuclear Agriculture, P.Box-4, Mymensingh-2200, Bangladesh. 3. Immediate past position(s): Student 4. Graduate and postgraduate degrees and training: Both degrees have obtained from Bangladesh

Agricultural University. A four month training on Microbial technique from India and A six month training on Molecular Biology from United Kingdom.

5. Prizes/Awards/Honours (academic and research): Not applicable. 6. Elected Fellowships of Academies: International Atomic Energy Agency and Islamic Development

Bank fellowship. 7. Research Advisory Positions/Journal Editorship/Directorship of Boards 8. Membership of Institutional Committees: Life member: Indian Journal of Microbiology, Member:

Krishibid Institution, Bangladesh, Bangladesh Microbiology Society. B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: I have been working in the field

of Biological Nitrogen Fixation research since 1999. 2. Area(s) of Expertise: Biological Nitrogen Fixation. 3. Number of peer-reviewed publications: 7 4. List of 5-10 most significant publications:

1. PodderA.K, Rashid, M. H and Hossain M.B. 2001. Effect of Bradyrhizbobial strain and nitrogenous fertilizer on groundnut production. Bangladesh J. Microbiol. 18(1): 9-14. 2. Rashid, M.H, M. Islam M. Z and Chowdhury, S.2002. Performance studies of some Bradyrhizobium isolates on growth and yield of Mungbean (Vigna radiata). Bangladesh J. Environ. Sci. 8: 172-178. 3. Rashid,M.H. Sattar,M.A., Islam,M.R and Young, J.P.W. Young. 2006. Genotypic Characterization of Lentil Rhizobia isolated from Bangladesh. Accepted in the 15th International Congress on Nitrogen fixation, Cape Town, South Africa-2007.

5. Patents (PCT applications/granted): Not applicable. 6. Competitive Research Grants obtained: Senior research fellowship from the ministry of Science and

technology. 7. Size and qualifications of research team supervised: Not applicable. 8. National and International Collaborations: Not applicable. 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): Gas

Chromatography, Bio-log for bacteria detection, PCR machine, DNA hybridization System, NOI-7 N15 analyzer and skilled manpower.

C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Currently we are train up a scientist from Sugarcane Research Institute under a collaboration program.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh?: We will be avail to develop better bio-fertilizer for problem areas in Bangladesh using biotechnological approaches.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes.


5. Would you consider returning to Bangladesh or spending substantial time there? Yes.If so, what conditions would induce you to return? Good working environment such as goods inputs (Chemicals and equipments) and freedom for conducting research.

Local scientists

179

Md. Israque Hossain Ansari A. Biosketch of Participants: 1. Name with academic title:- Md. Israque Hossain Ansari 2. Current position with affiliation, address and contact details :- Senior Scientific Officer, Institute of

Nuclear Medicine and Ultrasound, Bangladesh Atomic Energy Commission, 7th-10th Floor, Block-D, BSMMUCampus, Shahabag, Dhaka-1000.

3. Immediate past position(s):- Scientific Officer. 4. Graduate and postgraduate degrees and training: - B.Sc (Hons) Applied Chemistry, M.Sc

Biochemistry. IAEA Fellowship Training in Australia, India and Pakistan. 5. Prizes/Awards/Honours (academic and research) 6. Elected Fellowships of Academies 7. Research Advisory Positions/Journal Editorship/Directorship of Boards 8. Membership of Institutional Committees: - Member of Bangladesh Atomic Energy Scientist

Association (BAESA) And Society of Nuclear Medicine, Bangladesh (SNMB). B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research:-Estimation of Hormones,

Vitamins and Drugs using Radio nuclides , In vivo Function Study with radionuclides,Imaging various organ of the body using Radiopharmaceuticals, Radiotherapy to Cancer Patients.

2. Area(s) of Expertise: - Radioimmunoassay and Immunoradiometricassay of Invitro Laboratory of Nuclear Medicine.

3. Number of peer-reviewed publications 4. List of 5-10 most significant publications :-( 1). Schilling Evaluation- A Dual Isotope Test for

Vitamin B12 Malabsorption.Original article published in Bangladesh Journal of Medicine,1998;9(2):57-59. (2). Radiochemical Purity Monitoring: How important it is for Commonly Used 99mTc-Labeled Radiochemical. Published in Journal of Nuclear Medicine, Volume 9, Number1, January2006, and Page 27-33. (3). Computerized Quality Control of Radioimmunoassay (RIA) Performance in the Invitro Laboratory of INMU.Abstract, Published in World Journal of Nuclear Medicine, 2007.(4). The comparison Study of the CIAE TSH IRMA kit And the Neonatal TSH IRMA kit. Submitted in Journal of Nuclear Medicine, Bangladesh. (5). Half life Determination of 99mTc by Measuring Activity in dose Calibrator. Submitted in Journal of Nuclear Medicine, Bangladesh.

5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained 7. Size and qualifications of research team supervised 8. National and International Collaborations: M.Phil in Nuclear Medicine Under Dhaka University, and

Nuclear Medicine Facilities With Collaboration of International Atomic Energy Agency,Viena,Austeria

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?):- RIA & IRMA Facilities with γ-counter, PCR, and γ-camera, SPECT, & Ultra sonogram.

Local scientists

180

Mohammod Hossain

A. Biosketch of Participants: 1. Name with academic title: Mohammod Hossain, MS (in Plant Pathology) 2. Current position with affiliation: Senior Scientific Officer, working in molecular biology lab of plant

pathology division on disease resistance. Address and contact details: SSO, Plant Pathology Division, Bangladesh Rice Research Institute,

Gazipur, Bangladesh. Tel- 029259401-5 & 029257501-5 extn- 589 (O), 214 (R), email- [email protected], [email protected]

3. Immediate past position (s): Scientific Officer 4. Graduate: B. Sc. Ag.; postgraduate: MS (in Plant Pathology) both from Bangladesh Agricultural

University, Mymensingh, Bangladesh.

Training received:

Duration Organization

Month Day

Name of Programme and Year

(a) Abroad: University of Warwick, UK

6.0 Application of modern molecular biotechnological tools for fungal pathogens diversity and diagnostic, 2004-5.

CABI, UK 2.0 Training on Molecular Biology Techniques, 2002.

IRRI, Philippines

1.0 Integrated nutrient management (DINMOD), 2001.

IRRI, Philippines

1.5 Rice Seed Health for Crop Management, 1999.

(b) In Country:

BRRI 05 Hybrid seed production, 2006 BRRI

Section 4.02 June 02-July 04

Trained as molecular biologist under supervision of Dr. Conrad Stevens, IRRI, 2002-04.

BRRI 1.0 Introductory course in molecular biology, 2002

BRRI 03 Breeder seed production and preservation technique of rice,2002

BRRI 03 Identification, sampling and data collection on ShB Disease,2001

BARD 3.5 Foundation Training (including computer course, motor driving and office management), 2000

BRRI 2.0 Rice production, communication and office management, 1998

5. Prizes etc: Two awards in on job training– Distinction at IRRI, 1999 and First Chairman awards at

BARD, 2000.

Local scientists

181

6. Elected Fellowships of Academic: Received fellowship from Science and technology during MS study.

7. Research Advisory Position etc.: - 8. Membership: Life member of Bangladesh Phytopathological Society and Krisibid Institution of

Bangladesh. Member of BRRI Scientists Association. Associate member of Botanical Society of Bangladesh.

B. Research and Research Management Experience: 1. Major project area (s): Molecular study of rice pathogens. In the disease screening nursery of BRRI,

Bangladesh, breeding materials are screened against disease only by natural infection of the pathogen present in the nursery area. But this type of disease screening may not provide broad spectrum of resistance of breeding materials. The breeding lines those can show resistance against mixed populations of strains of a certain pathogen in screening program will be well established. Others are epidemiology of pathogen, yield loss due to pathogen, IPM, seed health management, ICNM.

2. Area (s) of Expertise: Plant pathology, Molecular Biology 3. Number of peer-reviewed Publication: 4. List of Most significant Publication:

i. Hossain, M., Conrad Stevens, M. A. Taher Mia, M. M. Kamal, Sarah Elliot and Steven Wayne. 2003. Identification of rice seed associated bacteria and molecular standardization using Denaturing Gradient Gel Electrophoresis. Bangladesh J. Plant Pathol. 19 (1 & 2): 39-44.

ii. Taher, M. A. T, Conrad Stevens, M. Mostafa Kamal, M. Hossain, M. Salim Mia, Saidur Rahman and J. A. Begum. 2003. Storage experiment and molecular activities at BRRI. The paper presented in the Review and Planning Workshop of rice seed health improvement sub-project held at BRRI, Gazipur during 22-23 April 2003.

iii. Hossain, M., Conrad Stevens, M. M. Kamal and M. A. Taher Mia. 2004. Molecular methods for identifying rice seed borne pathogen. The paper presented in the Thursday Seminar held at BRRI, Gazipur on 26 February 2004.

iv. Hossain, M., Mia, M. A. T., Kamal M. M. and Hossain, M.A. 2004. Standardization of DNA fingerprinting methods to identify genetic variability of Fusarium moniliforme. Submitted to the Journal of Microbiological World, India for publication.

v. Kamal, M.M and Hossain, M. 2007. Use of biotechnology and development of transgenics for crop disease management. A paper of National workshop on ‘Strategic intervention in plant pathology research in Bangladesh’ held on 11-12 February 2007 at BARI, Gazipur. Presented and excepted for publication in the proceedings.

vi. Hossain, M., M.A.Mazid, M.A.Begum, M.A.Kader and B.Sikdar. 2001. Effect of variety and seedling age on the yield of hybrid rice. Bangladesh J. genet. biotechnol. 2 (1 & 2): 09-14.

vii. Hossain, M. and M.A.T.Mia. 2001. Management of sheath blight disease of rice under farmer’s field condition. Bangladesh J.Plant Pathol. 17 (1 & 2): 13-16.

viii. Hossain, M., M.U.Ahmad, N.Ahmed, M.Abul Hossain and M.A.Alim. 2002. A study on control of root-knot (Meloidogyne javanica) of wheat. Indian Agric.46 (1 & 2): 121 -128.

ix. Hossain, M., M. A. Mazid, M. A. Kader, M. M. Kamal, M.A.T. Mia and I. U. Mollah. 2003. Effect of soil solarization and nematicide on soil parasitic nematode in direct seeded rice-wheat system. The Agriculturists 1(1): 47-52.

x. Hossain, M., M. A. Mazid, B. Karmaker, M. M. Kamal, M. Sh. Islam, M. A. Ali and M. A. Zami. 2004. Agronomic management of hybrid rice for better yield. Bangladesh Agron. J. 10 (1&2): 23-30.

5. Major Equipment/Facilities in Bangladesh: Chemical store, Microbalance, Fume hood, Incubators, Shaker, Freezers, Autoclave, Oven, Still, Flow cabinet, Safety cabinet, Water distillation plant, Microwave oven, Water baths with controlled temperature, Vacuum desiccators, Freeze-drier, Microcentrifuges, pH meter, Driblock heating blocks, Shelves, Ice maker, Dehumidifier, Flammable liquid store, PCR machines, Gel electrophoresis, U.V. Transilluminator and Chemicals for doing molecular researches. These may be available after the permission of the authority.

182

Md. Mozammel Hoq, Ph.D A. Biosketch of Participants: 1. Name with academic title: Md. Mozammel Hoq, Ph.D

Current position with affiliation, address and contact details: Professor, Dept. of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh Tel. 9661920-59/7733, 4402, 5400®, 8613479®, 01717083673, Email: [email protected] 2. Immediate past position(s): - 3. Graduate and postgraduate degrees and training

B.Sc (Hons) M.Sc(Dhaka), Ph.D(Nagoya), Visiting Professor, International Center for Biotechnology, Faculty of Engineering, Osaka University, Osaka, Japan, September 2001 to July 2002. Post doctoral research, Biochemical Engineering Division, National Center for Biotechnological Researches (GBF), Braunschweig, Germany, 1992-1994. International Training Program in Industrial Biotechnology, GBF, Braunschweig, Germany, 1991

One year International Post-Graduate University Course in Biotechnology in Osaka and Kyoto University, Japan, 1980

4. Prizes/Awards/Honours (academic and research) Dhaka University Merit Scholarship in M.Sc. (1975-76) 5. Elected Fellowships of Academies Science and Technology Research Foundation Fellowship, Ministry of Education, BD, (1977-78) UNESCO-Japan Govt. Fellowship(1980-81) Scholarship of Ministry of Science, Technology and Culture, Government of Japan (1981-1986) UNESCO-Germany Govt. Fellowship, 1992 Alexander-Von-Humboldt Research Foundation Fellowship, Government of Germany, 1992-1994 6. Research Advisory Positions/Journal Editorship/Directorship of Boards Bangladesh Journal of Microbiology 7. Membership of Institutional Committees i. Academic Council, Univ. of Dhaka ii. Faculty of Bioscience, D.U iii. Governing Body, Bhashani Medical College, Uttara, Dhaka B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research 2. Area(s) of Expertise : Microbial Biotechnology: Enzyme & Fermentation Biotechnology 3. Number of peer-reviewed publications: About 58 4. List of 5-10 most significant publications

• Nizam Uddin, M., Ilias, M., Rahman, A. and Hoq, M. M.. Compatibility and stability of alkaline protease from Bacillus licheniformis MZK-5 with commercial detergents. Bangladesh J.f Microbiolol. 23 (1), 19-23, (2006) • Hossain, M. S., Ilias, M. and Hoq, M. M.. Production and characterization of alkaline protease from novel Bacillus licheniformis MZK03. The Dhaka University Journal of Biological Sciences 15 (2), 139-147, (2006)

Local scientists

183

• Hoq, M. M., Siddiquee, K.A.L, Kawasaki, H. Seki, T. Keratinolytic activity of some newly isolated Bacillus species. J. Biol. Sciences 5(2) 193-200, (2005). • Azad, A. K., Shibata, H. and Hoq, M. M. Hide processing with alkaline protease from Bacillus sp. MA6. Bangladesh J. Microbiol. 19 (1 & 2), 67- 71, (2002). • Rahman, A.K.M.S, Kawamura, S. Hatsu, M., Hoq M.M. and Takamizawa, K. Physicochemical properties of a novel α-L-arabinofuranosidase from Rhizomucor pusillus HHT-1. Can J. Microbiol. 47: 767-772, (2001) • Hoq, M.M. and Deckwer, W.D. Cellulas-free xylanase by thermophilic fungi: A comparison on the production of xylanases by two Thermomyces lanuginosus strains. Appl. Microbiol. Biotechnol. 43: 604-609, (1995) • Hoq, M.M. Solomon. B.O., Hempel, C. Rinas, U. Deckwer, W.-D. The kinetics of cellulase-free xylanase excretion by Thermomyces lanuginosus RT9. J. Chem. Tech. • Hoq, M.M. Hempel, C. and Deckwer, W.-D. Cellulase-free xylanase by Thermomyces lanuginosus: Effect of agitation, aeration and medium components on production. J. Biotechnol 37: 49-58 (1994) • Alam, M.M. Gomes, I Mohiuddin, G and Hoq, M.M. Production and characterization of thermostable xylanases by Thermomyces lanuginosus and Thermoascus auranticacus grown on lignocelluloses. Enz. Microbiol. Technol. 16, 298-302 (1994) • Hoq, M.M., Yamane, T. and Shimizu, S. Continuous hydrolysis of olive oil by lipase in a microporous hydrophobic membrane bioreactor. J. Am. Oil Chem. Soc., 62 1017-1021 (1985).

184

Dr. Nazmul Ahsan A. Biosketch of Participants: 1. Name with academic title: Dr. Nazmul Ahsan 2. Current position with affiliation, address and contact details: Asst. Prof. Dept. of Genetic Engineering

and Biotechnology, Dhaka University, Dhaka-1000. 3. Immediate past position(s): Lecturer, Dept. of Genetic Engineering and Biotechnology, Dhaka

University, Dhaka-1000. 4. Graduate and postgraduate degrees and training: B.Sc. in Microbiology, M. Sc. in Microbiology,

Ph.D. in Tumor virology, Post doc. in Cancer Biology. 5. Prizes/Awards/Honours (academic and research): Japan Government (Monbukagakusho) scholarship.

International EBV Association Research Award, 2004 (student category). 6. Elected Fellowships of Academies: 7. Research Advisory Positions/Journal Editorship/Directorship of Boards 8. Membership of Institutional Committees

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research:

a. Prevalence and rapid diagnosis of Virus mediated cancer. b. Molecular basis and consequences of Arsenicosis. 2. Area(s) of Expertise: Analysis of viral infection and virus associated diseases, analysis of oncogene regulation, Growth

factors analysis, Cell culture etc. 3. Number of peer-reviewed publications: Five (5) 4. List of 5-10 most significant publications: i) Production of high-titer EBV recombinants derived from Akata cells using a bacterial artificial

chromosome system. T. Kanda, Yajima. M., Ahsan N., Tanaka M., Takada K. 2004. J. Virology. 78: 7004-15. ii) Viral transforming protein LMP1 plays a critical role in virus production. Nazmul Ahsan., Kanda T. Nagashima K., Takada. K. 2005. J. Virology. 79: 4415-4424. iii) Association between decision making autonomy and knowledge of HIV/AIDS prevention among ever-married women in Bangladesh. Aklimunnessa Khondoker, M.M.H. Khan, Nazmul Ahsan, M. Fazley Elahi Chowdhury, M. Kabir, Mitsuru Mori. 2006. J. Med. Sci. 6 (2): 155-163. iv) Characterization of Cellular Haemagglutination of Vibrio cholerae O139 Bengal Against Different Human Blood Groups. Nazmul Ahsan, Hasan, N.A., Kamal S.M.M., Bashar S.A.M.K., Miyoshi, S., Shinoda, S., M. Alam. 2005. B. J. Med. Sci. 11:96-100. v) Case-control study of arsenicosis in some arsenic contaminated villages of Bangladesh. MMH Khan , Khandoker Aklimunnessa, Nazmul Ahsan, M Kabir, Mitsuru Mori. 2006. Sapporo Med. J. Vol.76.

5. Patents (PCT applications/granted): Not applicable 6. Competitive Research Grants obtained: Not applicable 7. Size and qualifications of research team supervised: Not applicable 8. National and International Collaborations: Among different departments of Dhaka University.

Local scientists

185

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): Laminar airflow, Incubator, CO2 incubator, Centrifuge machine, PCR machine, Florescent microscope, Gel-doc machine etc.

Mohammad Shahnoor Hossain


1. Name with academic title: Mohammad Shahnoor Hossain 2. Current position with affiliation, address and contact details:

Lecturer, Department of Genetic Engineering and Biotechnology, University of Dhaka,Dhaka-1000 3. Immediate past position(s):Student 4. Graduate and postgraduate degrees and training:B.Sc(honours)and MS 5. Prizes/Awards/Honours (academic and research):Dean’s award 6. Elected Fellowships of Academies: Not applicable 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Not applicable 8. Membership of Institutional Committees Not applicable B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Microbial enzymes in leather

processing and bioremediation of chromium pollution caused by tannery effluent 2. Area(s) of Expertise: Enzymology 3. Number of peer-reviewed publications: Three 4. List of 5-10 most significant publications: Not applicable 5. Patents (PCT applications/granted): Not applicable 6. Competitive Research Grants obtained: Not applicable 7. Size and qualifications of research team supervised: Not applicable 8. National and International Collaborations: Not applicable 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?)

Local scientists

186

Dr Md Shamsher Ali

A. Biosketch of participants: 1. Name with academic title: Dr Md Shamsher Ali 2. Current position with affiliation, address and contact details: Chief Scientific Officer, Biotechnology Division, BRRI, Gazipur 1701. Tel. 9263729, Fax:9261110, Mob:01715158031 3. Immediate past position (s): Principal Scientific Officer 4. Graduate and postgraduate degrees and training

a. Degree Degree Board/University Year Ph D University of Newcastle upon Tyne, UK 2002 M.Sc (Ag) (Genetics & Plant Breeding

Bangladesh Agricultural University (BAU), Mymensingh

1983

B.Sc Ag. (Hons.) BAU, Mymensingh 1981

b. Training:

Course Duration Year Venue Biotechnological issues and Risk communication workshop

19-20 June 2005 BRAC Inn Centre, Bangladesh (Organised by BARC).

Workshop on review and evaluation of experimental field trials of transgenic crops

23-25 May 2005 BRAC Inn Centre, Bangladesh (Organised by BARC).

Training course on DNA fingerprinting 9 days 2004 International Rice Research Institute, Philippines (IRRI)

Rice Cultivation Technology 10 months 1994 Tsukuba, Japan Research Planning and evaluation 2 weeks 1989 Bangladesh Agricultural Research

Council Genetic Evaluation and Utilization, Deepwater Rice

4 months 1987 .IRRI, Philippines

Rice production, research management and administration

4 months 1983 Bangladesh Rice Research Institute

Seed cane inspection and protection Two weeks 1983 Sugarcane Research Institute, Bangladesh

Administration, office management and communication

10 days 1983 BAU, Mymensingh

5. Prizes/Awards/Honours (academic and research): Awarded grants for perusing PhD. 6. Elected fellowships of Academies: Not applicable 7. Research Advisory positions/Journal Editorship/Directorship of Boards: Not applicable 8. Membership of Institutional Committees: Member of Varietal Development Committee, Member Secretary, Institutional Biosafety Committee

Local scientists

187

B. Research and Research Management Experience: 1. Major project area (s); with very brief description of current research: Environmental control of plant stress tolerance, development of high grain yield varieties,

Development of stress tolerant variety. 2. Area (s) of Expertise: Functional genomics, gene isolation 3. Number of peer-reviewed publications: Not allicable 4. List of 5-10 most significant publications:

1. MS Ali, MA Salam, ME Hoque, MS Kabir, MS Islam , S Sultana, HU Ahmed,, S Khatun and BAA Mustafi (2006) Breeding and adoption of Boro varieties in Bangladesh. Int. J. Sustain. Agril. Tech. 2 (4):61-68. 2. MA Latif, MY Omar, SG Tan, SS Siraj and MS Ali (2005) Inheritance studies of RAPD-PCR molecualr markers in two sympatric populations of brown plant hopper, Nilaparbata lugens (Stål). Int. J. Sustain. Agril. Tech. 1(6):63-68. 3. ME Hoque,. MA Hossain, TL, Aditya, MKhalequzzaman and M. S Ali. (2005). Genotype-environment interaction and stability analysis in anther culture derived rice (Oryza sativa) genotypes. Bangladesh Journal of Plant Breeding and Genetics (in press). 4. MS Ali, S Khatun, MK Bashar, MS Alam, D Purba, M Kawase, K Okuno and S Kiyosawa (2004) Genetic Analysis for Two Components of Field Resistance: Lesion size and Number, to Rice Blast. Online J. Biol., Sci., 6(9):900-915. 5. Basher MK, K Akhter, KM Ittekharuddin and MS Ali (2003) Genetics of leaf water potential and its relationship with drought avoidance components in rice (Oryza sativa L.). Online J. Biol., Sci., 3(9):760-765.

5. Patents (PCT applications/granted): Not Applicable 6. Competitive Research Grants obtained: Not Applicable 7. Size and qualifications of research team supervised:

Development of stress tolerant variety, Development of high grain yield varieties. 8. National and International Collaborations: Collaborating with IRRI on Golden rice Network 9. Major Equipment/facilities in Bangladesh (will these be available to other Researchers?): All general

equipments are available in the laboratory and is available for any interested researchers.

188

Shanchita Rahnuma Khan A. Biosketch of Participants: 1. Name with academic title: Shanchita Rahnuma Khan 2. Current position with affiliation, address and contact details: Lecturer, Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000. Tel: 9661920 Ext: 7818 (W) 8611217 (H) 01714200899 (M) Email: [email protected] 3. Immediate past position(s): Lecturer, Department of Microbiology, Primeasia University, Banani, Dhaka 4. Graduate and postgraduate degrees and training: BSc (Hons) in Microbiology, University of Dhaka MSc in Microbiology, University of Dhaka MSc (by Research), University of New England, Armidale, Australia 5. Prizes/Awards/Honours (academic and research): ASBMB student award for poster presented at the Yeast: Products and Discovery (YPD) Meeting

held in Melbourne, Australia in 2002 6. Elected Fellowships of Academies: N/A 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: N/A 8. Membership of Institutional Committees: N/A B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: None 2. Area(s) of Expertise: Microbiological quality control of frozen fish. Oxidative stress response in yeasts. 3. Number of peer-reviewed publications: 2 4. List of 5-10 most significant publications: Khan SR & Khan SI (2003). Bacteriological quality of locally available and exportable frozen shrimp

of Bangladesh. Dhaka University Journal of Biological Sciences 12(1): 1-6 Khan SR, Talukder KA & Khan SI (2004). Plasmid profiles and toxicity of Escherichia coli isolated

from frozen shrimp in Bangladesh. Dhaka University Journal of Biological Sciences 13(2): 173-179 5. Patents (PCT applications/granted): N/A 6. Competitive Research Grants obtained: N/A 7. Size and qualifications of research team supervised: N/A 8. National and International Collaborations: N/A 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): None

Local scientists

189

S. M. Zakiur Rahman


1. Name with academic title S. M. Zakiur Rahman Scientific Officer

2. Current position with affiliation, address and contact details Scientific Officer Bangladesh Fisheries Research Institute Marine Fisheries and Technology Station Cox’s Bazar – 4700. Email: [email protected] Mobile: +8801710214046

3. Immediate past position(s) Student M.S. in Biotechnology Biotechnology Department Bangladesh Agricultural University Mymensingh-2202


Academic Career:

Name of the examination

Board/ University Year Division/

Class/Grade Marks (%)/ GPA Obtained

S.S.C Rajshahi Board 1996 First 78.10 H.S.C Rajshahi Board 1998 First 66.90 Bachelor of Science in Fisheries (Honors)


2002 (held in 2004) First 65.11

Master of Science in Biotechnology


2005 First / A- 73.60 / 3.68 (out of 4)

Training:

No. Name of the Course Institute Duration 1. Training Course on

“Aquaculture and Extension” Mymensingh Aquaculture and Extension Project (MAEP), Mymensingh

6 days

2. Training Course on Fisheries and Aquaculture Research

Bangladesh Fisheries Research Institute (BFRI), Mymensingh

6 days

3. Extension Field Trip Department of Agricultural Extension and Education at Bhaluka, Mymensingh

7 days

Local scientists

190


• Fellowship of Bangladesh Agricultural University Research System (BAURES) under the supervision of Dr. Md. Mukhlesur Rahman Khan, Head, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University • Fellowship of ICLARM under the supervision of Dr. Md. Mukhlesur Rahman Khan, Head, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University

6. Elected Fellowships of Academics Obtained Part-time general Fellowships of National Science and Information Communication Technology in 2005.

7. Research Advisory Positions/Journal Editorship/Directorship of Boards Not applicable

8. Membership of Institutional Committees Bangladesh Fisheries Research Forum (BFRF), Bangladesh (2006) B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research

PCR-RAPD analysis using nuclear DNA of catla (Catla catla) is also conducted in the fishereis biology and genetics department of Bangladesh Agricultural University. Here I have worked under the supervision of Dr. Md. Mukhlesur Rahman Khan, Head, Department of fishereis biology and genetics. Riverine and hatchery stocks are analyzed to know the genetic variation of stocks for aquaculture program. Four population were studied namely Halda, Jamuna, Padma and one Hatchery population where percentage of polymorphic loci and Nei’s gene diversity (Nei, 1973) were 41.94% and 0.164 for Halda; 41.94% and 0.147 for Jamuna; 45.16% and 0.176 for Padma; and 35.48% and 0.154 for Hatchery populations respectively, revealing relatively higher level of genetic variation in the Padma population.

2. Number of peer-reviewed publications:

3. List of 5-10 most significant publications:

Full Length Paper

1) Parvez I., M.M.R.Khan, S.M.Zakiur Rahman and M .A.Alam. (2006). Present status and future potential of the gene pool of local sarpunti, Puntius sarana (Hamilton), J of Bangladesh Agril. Univ. 4(2): 319-324 2) Khan, M.M.R., N.R.U. Noor and S.M.Z. Rahman. 2005. Use of allozyme markers to determine the genetic structure of hatchery population of Thai pangas, Pangasius hypophthalmus of Jessore, Bangladesh. Bangladesh J. Fish. Res., Special issue 9(1): 7-8. 3) Khan M.M.R., M.S. Alam, S.M.Z. Rahman and I. Parvez (2003). Genetic variation of Tilapia strains inferred by allozyme marker. Journal of Molecular Biology and Biotechnology Journal, 1(1&2): 59-62. Short Length Paper (Proceedings) 1) Noor, A.M., M.M.R. Khan, S.M.Z. Rahman and I. Parvez. 2006. Growth and morphological comparison between local and Thai koi Anabas testudinius (Bloch) in Bangladesh. 2nd Fisheries Conference and Research Fair 2006, BFRF, Abstracts. p 6. 2) Rahman, S.M.Z., M.M.R. Khan, M.S. Islam, I. Parvez and M.S. Alam. 2006. Genetic variation study in wild and hatchery population of Catla catla (Ham.) using randomly amplified polymorphic DNA (RAPD) markers. 2nd Fisheries Conference and Research Fair 2006, BFRF, Abstracts. p 11-12. 3) Parvez, I., M.M.R. Khan and S.M.Z. Rahman. 2006. Study for conservation of endangered local sarpunti Puntius sarana (Ham). 2nd Fisheries Conference and Research Fair 2006, BFRF, Abstracts. p 13-14.

4. Patents (PCT applications/granted): None

191

5. Competitive Research Grants obtained: None

6. Size and qualifications of research team supervised: None 7. National and International Collaborations: None 8. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) A

minimum facility is available to carry out Biotechnology related research in the field of Fish and shrimp Diseases, as well as, molecular marker based research to stock improvement of fish in Bangladesh.


(Information requested from Expatriate Scientists)-

1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so, please provide brief information. No

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? I am working on shrimp disease where PCR based research is going on. 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory?

Yes I can. 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Yes I need training relating to biotechnology 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? I need higher study like PhD but I must like to do research and teaching for my country Bangladesh. 6. Any other comments? I am a Scientific Officer of a Research Institute of Bangladesh. As a scientist, I

have an appeal to all that if there is any chance please try to help us for the development of Laboratory facilities in my Institute.

192

Abul K M Ekramoddoullah A. Biosketch of Participants: 1. Name with academic title

Abul K M Ekramoddoullah, Ph.D. 2. Current position with affiliation, address and contact details

Senior Research Scientist and Adjunct Professor Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada 506 West Burnside Rd, Victoria, British Columbia, V8Z 1M5, Canada [email protected]

3. Immediate past position(s) a. Research Associate, Department of Immunology, University of Manitoba, Canada b. Head, Quality Control Division, Squibb Pharmaceuticals, Dhaka, Bangladesh c. Senior Lecturer, Department of Biochemistry, Dhaka University, Bangladesh 4. Graduate and postgraduate degrees and training. BSc. (Physics, Chemistry and Mathematics; Dhaka University); M. Sc.(Biochemistry, Dhaka University); Ph. D. (Biochemistry, McGill University, Montreal, Canada) and received specialized training in quality control of pharmaceuticals and

management of pharmaceutical plants in England and Argentina 5. Prizes/Awards/Honours (academic and research); Distinguished Member ( awarded by International

Society of Plant Microbe-Interaction); Outstanding Research (awarded by the Canadian Society of Plant Pathology); Valuable Research Scholarly Contribution (awarded by the Department of Immunology and Medical Research Group for Allergy Research, University of Manitoba)

6. Elected Fellowships of Academies New York Academy of Sciences

7. Research Advisory Positions/Journal Editorship/Directorship of Boards Member of the Editorial Board, Tree Physiology, Ex-member of the WHO Allergen Nomenclature and Standardization Committees Ex-Chairmen of Science Council Graduate Students Scholarship Committee, Ex-member, Research Council Granting Agency External Reviewer, National Science Foundation (USA)

8. Membership of Institutional Committees d. Graduate Student Committee e. Research Scientist Promotion Committee

B Research and Research Management Experience: 1. Major project area(s); with very brief description of current research Our previous screening program at CFS-Victoria identified white pine seedlings with several

resistance mechanisms including dominant gene resistance (R). These are being used to build seed orchards. To improve and sustain the genetic resistance, it is essential that we understand the genetic and biochemical mechanisms of white pine defence against blister rust. In this project, we will investigate the biological function of the white pine R gene family for the ultimate purpose of the molecular understanding of the conifer defence mechanism against fungal infection, and pyramiding different resistance mechanisms into western white pine in forest breeding. We have also has identified more than 130 resistance gene analogues (RGAs) of the NBS-LRR family and an anti-blister rust anti-microbial peptide (PmAMP1), and a chitinase isoform associated with slow-canker growth resistance. RGAs will be used in a candidate-gene based approach to characterize the Cr2 gene in WWP. In this approach, a genetic map will be constructed, followed by isolation and characterization of Cr2 gene. In the Cr2 mediated signal transduction pathway we will characterize up-stream regulatory genes e.g. transcription factors, particularly those inducible by the rust fungus.

Oral presenters

193

This transcription factor (the “master switch”) will help to induce increased defence response in white pine to the rust fungus. The gene regulation of PmAMP1 and chitinase isoform will be studied to develop markers for SCG. Ultimately, the molecular and genetic basis of resistance and susceptibility to white pine disease will be elucidated.


Proteomics and genomics 3. Number of peer-reviewed publications

Ninety seven 4. List of 5-10 most significant publications

Ekramodddoullah, A.K.M.; Tan, Y.; Yu, X.; Taylor, D. and Misra, S.M. 1999. Cloning, sequencing and characterization of a gene encoding a novel secreted fungal protein discovered in white pine needles infected with blister rust fungus, Cronartium ribicola. Can. J. Bot. 77: 800-808. Yu, X.; Ekramoddoullah, A.K.M.; Taylor, D.W. and Piggott, N. 2002. Cloning and characterization of a cDNA of cro r I from the white pine blister rust fungus, Cronartium ribicola,. Fungal Genetics and Biology. 35:53-66. Liu, J-J. and Ekramoddoullah, A.K.M. 2003. Root specific expression of a western white pine PR-10 gene mediated by different promoter regions in transgenic tobacco. Plant Mol. Biol. 52:103-120. Mattheus, N.; Ekramoddoullah, A.K.M. and Lee, S.P. 2003. Isolation of high quality RNA from white spruce tissue using a three stage purification and subsequent cloning of a transcript from the PR-10 gene family. Phytochemical Analysis. 14:209-215. Liu, J-J.; Ekramoddoullah, A.K.M. and Podila, G.K. 2003. A MAD-Box gene specifcally expressed in the reproductive tissues of red pine (Pinus resinosa) is a homologue to floral homeotic gene with C-function in angiosperms. Physiol. Mol. Biol. Plants. 9:197-206. Liu, J-J. and Ekramoddoullah, A.K.M. 2003. Isolation, genetic variation and expression of TIR-NBS-LRR resistance gene analogue from western white pine (Pinus monticola Dougl. Ex. D. Don). Molecular Genetics and Genomics. 270:432-441. Liu, J-J.; Ekramoddoullah, A.K.M.; Piggott, N. and Zamani, A. 2004. A pathogen/wound-inducible PR10 promoter from Pinus monticola: molecular cloning and characterization in transgenic Arabidopsis plants. Planta. 221: 159-169. Liu, J-J.; Ekramoddoullah, A.K.M. and Zamani, A. 2005. A class IV chitinase is up-regulated by fungal infection and abiotic stresses and associated with slow-canker-growth resistance to Cronartium ribicola in western white pine (Pinus monticola, Dougl. Ex D. Don). Phytopathology 94:1235-1243. Ekramoddoullah, A.K.M., Liu, J-J and Zamani, A. 2006. Cloning and characterization of a putative antifungal peptide gene (Pm-AMP1) in Pinus monticola. Phytopsthology 96: 164-170. Liu, J-J.; Ekramoddoullah, A.K.M. Hunt, R.S. and Zamani, A. 2006. Identification of random amplified polymorphic DNA markers linked to major gene (Cr 2) for resistance to Cronartium ribicola in Pinus monticola. Phytopathology. 96: 395-399.

1) Publication in Refereed Conference Proceedings: 5. Patents (PCT applications/granted): 6 patents 6. Competitive Research Grants obtained 17 million dollars (over a period of 30 years) 7. Size and qualifications of research team supervised 18 graduates students (M.Sc. and (Ph. D.), 7 PDF, 8 visiting scientists) 8. National and International Collaborations USA, Sweden, Norway, Poland 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) None

194

Dr. Md. Abul Kalam Azad A. Biosketch of Participants:

1. Name with academic title: Dr. Md. Abul Kalam Azad 2. Current position with affiliation, address and contact details: Foreign Researcher, Dept. of Life

Science and Biotechnology, Shimane University, Shimane 690-8504, Japan, e-mail: [email protected]

3. Immediate past position(s): Assistant Professor (Currently on Leave), Dept. of Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

4. Graduate and postgraduate degrees and training: B.Sc (Hons) and M.Sc. (Dept. of Microbiology, DU, Bangladesh), Ph.D. (Tottori University, Japan), Postdoc (Shimane University, Japan)

5. Prizes/Awards/Honours (academic and research): (1) JSPS Fellowship for postdoctoral research (2) Monbusho Scholarship for Ph.D. research (3) Dhaka University Scholarship for first class (4) Dean award from Faculty of Biological Sciences, DU, Bangladesh (5) Govt. first grade scholarship from class six to B.Sc. (Hons) levels.

6. Elected Fellowships of Academies 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Technical editor, Several

Journals published by Asian Network for Scientific Information. 8. Membership of Institutional Committees B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research:

● Functional and expression analysis and molecular regulation mechanisms of Aquaporins, the water channel proteins, to elucidate their roles in Molecular Cell Physiology. Environmental factors stimulating cellular signal transduction through protein-protein interaction, post-translational modifications of proteins. Elucidation of genes for cellular physiology, and protein structure and functions. ● Studies about the molecular markers involved in the process of Program Cell Death (PCD) during natural as well as induced senescence or aging. ● Research on microbial proteinases with biotechnological potentiality for biodegradation or bioprocessing or bioconversion.

2. Area(s) of Expertise: Molecular Biotechnology and Recombinant Protein Technology, Signal Transduction, Microbial Enzymology

3. Number of peer-reviewed publications: 8 (eight) 4. List of 5-10 most significant publications:

● Azad A.K., Sawa Y, Ishikawa, T. and Shibata H. (2007) Temperature-dependent stomatal movement in tulip petals controls water transpiration during flower opening and closing. Annals of Applied Biology, 150 (1): 81-87. Blackwell Publishers. ● Azad A.K., Sawa Y, Ishikawa, T. and Shibata H. (2006) Purification and characterization of protein phosphates 2A from petals of the tulip Tulipa gesnerina. Journal of Biochemistry and Molecular Biology, 39 (6): 671-676. PubMed available. ● Azad A. K., Sawa Y., Ishikawa T. and Shibata H. (2004) Phosphorylation of plasma membrane aquaporin regulates the temperature-dependent opening of tulip petals. Plant & Cell Physiology 45(5): 608- 617. Available in PubMed. ● Azad A. K., Sawa Y., Ishikawa T. and Shibata H. (2004) Characterization of protein phosphatase 2A acting on phosphorylated plasma membrane aquaporin of tulip petals. Bioscience Biotechnology and Biochemistry, 68(5): 1170-1174. Available in PubMed.

Oral presenters

195

● Azad A.K. Ishikawa Takayuki, Sawa Y., Ishikawa Takahiro and Shibata H. (2007) Intracellular energy depletion triggers programmed cell death through DNA degradation and mitochondrial disintegration during petal senescence in tulip. Revised version submitted. ● Azad A.K. Maki K., Sawa Y., Ishikawa Takahiro and Shibata H. (2007) Molecular cloning, functional and expression analysis of four isoforms of plasma membrane aquaporin subfamilies of the tulip petal Tulipa gesnerina. Submitted. ● Hossain M. S., Azad A. K., Sayem S. M. A., Mostafa G. and Hoq M. M. (2007) Production and partial characterization of feather-degrading keratinolytic serine protease from Bacillus licheniformis MZK-3. Journal of Biological Sciences, In Press. ● Hosen M. J., Sarif D. I., M. Rahman M. M. and Azad A.K. (2006) Contamination of coliforms in different paper currency notes of Bangladesh. Pakistan Journal of Biological Sciences, 9(5): 868-870. ● Azad A. K., Shibata H. and Hoq M. M. (2002) Hide processing with alkaline protease from Bacillus sp MA6. Bangladesh J. Microbiology, 19(1&2): 67-71. ISSN 1011-9981 ● Azad A. K. and Hoq M. M. (2000) Production of alkaline serine protease from Bacillus sp. MA6. Bangladesh Journal of Microbiology, 17(2): 143-149.

5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained: 1 (Ministry of Education, Science, Sports and Culture, Japan) 7. Size and qualifications of research team supervised: 4 (Graduation) 8. National and International Collaborations 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh:


please provide brief information. I am trying to build up a collaboration with Shimane University and Okayama University, Japan

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? My research is especially in Molecular Cell Physiology, Molecular Biotechnology and Recombinant Protein Technology, which exploits molecular approaches. When I will return in my teaching profession in Bangladesh, this expertise will help me to train up the students with practical knowledge, lead the research team, generate biotechnological approaches, and contribute in the development of potential Biotechnology. I would be able to collaborate with other laboratories in abroad that would open research scope in home. As I am directly associated with Biotechnology, I could try to encourage the industrial sector to be associated with biotechnological research and funding, and products. I could be associated with the Biotechnology family in Bangladesh to solve many obstacles in the research of Biotechnology.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes, I am confident.

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? As I have position in Bangladesh, I am going to return just after six months to contribute permanently.

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? I am returning permanently.

6. Any other comments? I would like to share comments directly later. For example, strategically changes in funding, equipments and reagent imports.

196

Ahmad S. Islam A. Biosketch of Participants:

1. Name with academic title: Ahmad S. Islam 2. Current position with affiliation, address and contact details: Research Associate, Molecular, Cell and Developmental Biology University of Texas, Austin, TX78713 3. Immediate past position(s): Professor of Botany, Dhaka University (retired) 4. Graduate and postgraduate degrees and training: Ph.D. from Manchester University; postdoctoral

from Cornell University, Ithaca, NY and University of California at Davis, USA 5. Prizes/Awards/Honours (academic and research): Currie Memorial Prize from Manchester

University, Ekushey Padak in Education, President’s Gold Medal in Agriculture, BAS Gold Medal in Biology.

6. Elected Fellowships of Academies: Fellow of Bangladesh Academy of Sciences (BAS), Fellow of Islamic Academy of Sciences (IAS)

7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Chief Editor, J. Tissue Culture and Biotechnology; also worked as Chief Editor Bangladesh J. of Botany, Chief Editor Pakistan J. of Botany

8. Membership of Institutional Committees: Currently none B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Cloning of genes of agronomic

importance in jute species, Corchorus olitorius from cDNA library 2. Area(s) of Expertise: Genetic Engineering 3. Number of peer-reviewed publications: Ten 4. List of 5-10 most significant publications: See the footnote marked with an asterisk 5. Patents (PCT applications/granted): None 6. Competitive Research Grants obtained: Pakistan and then Bangladesh Government; grants from

USAID to visit a number of important labs after jute project was short-listed. It was dropped from the final list.

7. Size and qualifications of research team supervised: Twenty-two students did their Ph.D. under my sole or joint supervision

8. National and International Collaborations. N/A 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) N/A C. Capacity Development and Training Activities in Bangladesh:


1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so, please provide brief information. Dr. K. Sathasivan at University of Texas, Austin and myself as his research associate are doing joint research project on jute cDNA library construction with Professor Haseena Khan, Department of Biochemistry and Molecular Biology, Dhaka University. USDA is expected to fund the project soon.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Molecular cloning of useful genes of jute will be a first step toward improvement of quality of jute fibers and production of flood-, drought tolerant and disease and pest resistant jute varieties.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes.

Oral presenters

197

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? Yes, in any capacity suitable to my qualifications and experience.

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? I have decided to return and serve Bangladesh in this capacity as long as I live

6. Any other comments? If India and Pakistan can build up a strong team of Biotechnology, we can do

it. We have manpower resources distributed all over the world (See database of GNOBB using its search engine). Wheel need not be reinvented; whatever strategy has worked for India and Pakistan to bring back a substantial scientific workforce from abroad, may also apply in Bangladesh with some modifications. Time is running out. The biotechnology projected was submitted early 1973 and now it is 2007. The National Institute of Biotechnology has the building with some partially equipped labs, where only a handful of junior scientists have been working with hardly anyone to guide them. *Islam A. S. and A. Rashid (1960) First successful hybridization between the two jute yielding species. Nature 185 : 258-259. Islam A. S. and A. Rashid (1961) A new jute hybrid. J. Heredity 52: 287-291. Islam A. S. (1964) A rare hybrid combination through application of hormone and embryo culture. Nature 201 : 320. Islam A. S. and R. Mughal (1969) Corchorus pascorum : Transmission of chemically induced fruit formation with environmental change. Science 164: 315-316. Islam A.S. and Sharif A. (1970) Breeding of cotton Varieties for Jassid resistance. Canadian Journal Genetics and Cytology 12:454-460 Islam A. S. and M. M. Haque (1973) Improvement of jute through interspecific hybridization. SABRAO Jour 5: 75-82. Islam A. S., M. M. Haque and M. B. A. Dewan (1975) Attempt to produce photo-neutral strain of jute through interspecific hybridization. Japan. J. Breed. and Genet. 25 (6) : 349-354. Haque M. M., L. A. Khan, M. Rahman and A. S. Islam (1980) Attempt to overcome crossibility between the two jute species by use of a wild parent. Japan J. Breed. and Genet. 30: 231-235. Islam A. S., M. Haque and M.S. Haque (1980) Fibre-bearing Potentiality of two jute hybrids. Indian J. Genetics & Plant Breeding 40(3) 578-580. Seraj Z.I, Sarker AB and Islam AS (1992) Plant regeneration in a jute species (C. capsularis) and its possible relationship with glyoxalase-1. Plant Cell Rep. 12:29-33. Islam A. S., M.M. Haque, M.I. Hoque, and Z.I. Seraj (1992) Tissue Culture and micropropagation of jute (Corchorus species), In: Biotechnology in Agriculture and Forestry, Vol 19, High Tech and Micropropagation III. Bajaj YPS (Ed.), Springer Verlag, Berlin, Heidelberg. pp. 505-526.

198

Ahmed Abdullah Azad A Biosketch of Participants: 1. Name with academic title Professor Ahmed Abdullah Azad PhD 2. Current position with affiliation, address and contact details Current Positions:

A. TWAS Research Professor, BRAC University, Dhaka, Bangladesh B. Programme Director and International Coordinator, OIC Drug Discovery and Development Network C. Honorary Professor of Medical Biotechnology, University of Cape Town D. Postal Address: 4 Chapel Court Doncaster Victoria 3108 Australia Email Address: [email protected] Telephone: +61 3 9848 4770

3. Immediate past position(s) Director of Research and Professor of Medical Biotechnology Faculty of Health Sciences, University of Cape Town (Till January, 2006) 4. Chief Research Scientist and Program Manager, Molecular Virology CSIRO Division of Molecular Biosciences, Melbourne (Till December, 1999) 5. Graduate and postgraduate degrees and training

B.Sc.(Hons.); First Class; Biochemistry, Dhaka University; 1967 M.Sc.(Thesis); First Class (First); Biochemistry, Dhaka University, 1968 PhD; Biochemistry and Molecular Biology; University of Toronto; 1970-1973 Canadian MRC Postdoctoral Fellowship; Molecular Biology; University of Toronto; 1973-75

6. Prizes/Awards/Honours (academic and research) The CSIRO Chairman’s Medal for Exceptional Achievement, 1997 (for development and commercialisation of a recombinant subunit vaccine against an immunosuppressive viral disease) Vice-Chancellors Prize for Rank 1 in Biochemistry (M.Sc), 1968 Dr. Bart Rispens Memorial Award for Avian Pathology, 1991 Undergraduate Talent Scheme Scholarship, 1964-67 Graduate Talent Scheme Scholarship, 1967-68 University Merit Scholarship, 1967-68 Canadian Commonwealth Post-graduate Scholarship (1970-73), Canadian MRC Postdoctoral Fellowship, 1973-75

7. Elected Fellowships of Science Academies Fellow, TWAS (Academy of Science of the Developing World) (2002) Member, ASSAf (Academy of Sciences of South Africa) (2001) Fellow, RSSA (Royal Society of South Africa) (2002) Fellow, IAS (Islamic-World Academy of Sciences) (2000) Fellow, BAS (Bangladesh Academy of Science) (2005)

8. Research Advisory Positions/Journal Editorship/Directorship of Boards Director, Board of the Medical Research Council of South Africa (appointed by the South African Minister of Health, August 2004) Member, Council of Scientific Advisors, ICGEB (International Centre for Genetic Engineering and Biotechnology) Member, Scientific Advisory Committee of SAAVI (The South African AIDS Vaccine Initiative) Member, Finance and International Programmes Committee, TWAS (The Academy of the Developing World)

Oral presenters

199

Member, Standing Panel on Biotechnology, TWAS Member, Technical Advisory Committee, COMSATS (Commission for Science and Technology for Sustainable Development in the South) Member, Scientific Coordination Committee of USHEPiA (a research network of eight universities in Sub-Saharan Africa) Visiting Professor and Scientific Advisor, Ambedkar Centre for Biomedical Research, Delhi University (New Delhi, India)) Visiting Professor and Scientific Advisor, Biomedical Research Laboratory, Gono University (Dhaka, Bangladesh) Visiting Professor and Scientific Advisor, HEJ Research Institute for Chemistry and Dr. Panjwani Centre for Molecular Medicine and Drug Research, University of Karachi (Karachi, Pakistan) Scientific Advisor, Arab School of Science and Technology (Damascus and Kuwait)

9. Membership of Institutional Committees University of Cape Town (UCT): (2000-2005) Chair, Faculty of Health Sciences Research Committee Member of the following Academic Bodies: UCT Senate; University Research Committee; Health Sciences Faculty Board; Faculty Executive Committee; Faculty Senior Management Team Director/Board Member of the following UCT Management/Advisory Boards: UCT Innovation Advisory Board; UCT Sports Science Centre Scientific Advisory Board; Chris Barnard Fund; UCT Lung Institute Board; Medicines Information Centre Member of the Project Implementation Committee and the Fund Raising Committee of the Institute of Infectious Disease and Molecular Medicine (IIDMM) Some Research Management and Capacity Building Activities at UCT: Played major role in the planning and establishment of the Institute of Infectious Disease and Molecular Medicine (IIDM) at the University of Cape Town (now the Third Component of ICGEB) Developed comprehensive Guidelines for the Conduct of Research and Research Management Procedures for the Faculty of Health Sciences Helped set up a financial planning process for recovery of full cost of research and generation of excess research funds for strategic purposes Helped develop strategies for Intellectual Property Development and Commercialisation of Research at UCT Carried out a comprehensive Audit of all research activities in the Faculty of Health Sciences, and developed strategies for addressing deficiencies identified by the Research Audit


Current Research and Research Management Focus: “Establishment of a Drug Discovery and Development Research Network in the scientifically lagging countries of Asia and Africa” (co-sponsors: TWAS, ICGEB, COMSTECH, IDB, IAS and AAS) Past Research Activities: Role of RNA-RNA interactions (5SrRNA-18SrRNA; mRNA-18SrRNA) in protein synthesis Yeast and plant molecular biology Cloning and sequencing of Influenza neuraminidase gene (this helped in solving the 3D structure of neuraminidase and the development of a rationally designed anti-influenza drug) Cloning and sequencing of other genes of Influenza Virus, Rotavirus, Potyvirus, and Birnavirus. Genetic improvement of cheese starter strains Development of new expression vectors for the high level production of recombinant proteins Molecular Biology of Infectious Bursal Disease Virus, and the development and commercialisation of recombinant subunit vaccine (this resulted in the award of the CSIRO Chairman’s Gold Medal for Exceptional Achievement in 1997) Role of HIV-1 accessory proteins Nef and Vpr in AIDS pathogenesis, and development of drugs (based on the structures and functions of Nef and Vpr) that slow progression to AIDS (R&D Syndicate funding: $7.5 million)

200

2. Area(s) of Expertise Biochemistry and Molecular Biology Genetic Engineering and Biotechnology Molecular Virology High-level expression and large-scale production of Recombinant Proteins Vaccine Development Discovery and Rational Design of Drugs 3. Number of peer-reviewed publications Over 130 publications in international peer reviewed and indexed journals. 4. List of 5-10 most significant publications

The following list of publications represent the range of research I have been involved in over 30 years: A. Azad, A.A. (1979). Intermolecular base-paired interaction between complementary sequences present near the 3’ ends of 5S rRNA and 18S (16S) rRNA might be involved in the reversible association of ribosomal subunits. Nuc. Acids Res. 7, 1913-1929. B. Azad, A.A., Elleman, T.C., Laver, W.G. & Ward, C.W. (1983). Sequence changes associated with antigenic shift and drift in influenza virus neuraminidase. The origin of pandemic influenza viruses. Proc. Int. Workshop, 1982, (Laver, W.G. ed.) Elsevier, New York, 59-76. C. Azad, A.A., Barrett, S.A. & Fahey, K.J. (1985). The characterisation and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology. 143, 35-44. D. Azad, A.A., Jagadish, M.N., Brown, M.A. & Hudson, P.J. (1987). Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus. Virology. 161, 145-152. E. Azad, A.A., Macreadie, I.G., Vaughan, P.R., Jagadish, M.N., McKern, N.M., Heine, H.G., Failla, P.P., Ward, C.W., Chapman, A. & Fahey, K. (1990). Full protection against an immunodepressive viral disease by a recombinant antigen produced in yeast. Vaccines 90 : modern approaches to new vaccines including the prevention of AIDS, (Brown, F., Chanock, R.M., Ginsberg, H.S., Lerner, R.A. eds.). Cold Spring Harbor Laboratory Press, New York, 59-62. F. Azad, A.A., McKern, N.M., Macreadie, I.G., Failla, P.P., Heine, H.G., Chapman, A., Ward, C.W. & Fahey, K.J. (1991). Physiochemical and immunological characterisation of recombinant host-protective antigen (VP2) of infectious bursal disease virus. Vaccine. 9, 715-722. G. Azad, A.A., Failla, P.P., Lucantoni, A.C., Bentley, J.D., Mardon, C.J., Wolfe, A.G., Fuller, K., Hewish, D.R., Sengupta, S., Sankovich, S.E., Grgacic, E., McPhee, D. & Macreadie, I.G. (1994). Large scale production and characterisation of recombinant human immunodeficiency virus type 1 Nef. J. Gen. Virol. 75, 651-655. H. Macreadie, I.G., Castelli, L.A., Hewish, D.R., Kirkpatrick, A., Ward, A.C. & Azad, A.A. (1995). A domain of human immunodeficiency virus type 1 Vpr containing repeated H(S/F) RIG amino acid motifs causes cell growth arrest and structural defects. Proc. Natl. Acad. Sci. USA. 92, 2770-2774. I. Arunagiri, C., Macreadie, I., Hewish, & Azad, A.A. (1997). A C-terminal domain of HIV-1 accessory protein Vpr is involved in penetration, mitochondrial dysfunction and apoptosis of human CD4+ lymphocytes. Apoptosis. 2, 69-76. J. Azad. A. A. (2000). Could Nef and Vpr proteins contribute to disease progression by promoting depletion of bystander cells and prolonged survival of HIV-infected cells. Biochem. Biophys. Res. Commun. 267, 677-685.

5. Patents (PCT applications/granted) i. IBDV Patents (all granted)

(i) Title: Cloning and expression of a host-protective immunogen of IBDV and its efficacy in vivo (1985). Inventors: Azad, A.A., Fahey, K.J. and Hudson, P.J. (ii)Title: Expression System (1987). Inventors: Macreadie, I.G., Vaughan, P.R. and Azad, A.A. c. (iii)Title: IBDV VP2 epitope recognised by virus neutralising and protective monoclonal antibodies (1987). Inventors: Azad, A.A., Jagadish, M.N., Fahey, K.J. d. (iv)Title: Production of IBDV VP2 in highly immunogenic form (1989). Inventors: Azad, A.A., McKern, N.W., Macreadie, I.G., Vaughan, P.R., Heine. H.G., Jagadish, M.N., Chapman, A.J. and Fahey, K.J.

201

ii. HIV Patents (The following PCT applications have also been granted) (v)International Patent Application : PCT/AU94/00254 Title: Therapeutic Compounds (Nef) Inventors: Azad, A.A., Curtain, C., Macreadie, I.G., Greenway, A. & McPhee, D. (vi)International patent Application : PCT/AU95/00169 Title: Therapeutic Compounds (Vpr) Inventors: Macreadie, I.G., Arunagiri, C. & Azad, A.A. (vii) International Patent Application : PCT/AU97/00640 Title: Cytotoxic Peptides (Nef) Inventors: Azad, A.A., Curtain, C., Lowe, M., Macreadie, I.G., Arunagiri, C., Baell, J., Matthews, B., Barnham, J., Norton, R. &Rivett,

6. Competitive Research Grants obtained National Biotechnology Program Research Grants Scheme, $700,000. 1985-88. Development of IBDV Vaccine. GIRD Biotechnology Grant, $150,000. 1988-89. Development of IBDV Vaccine. Arthur Webster Pty Ltd. $570,000, 1986-89. Commonwealth AIDS Research Grant, $146,000, 1989-92. Expression of HIV Regulatory Proteins for Function and Structure Determination Commonwealth AIDS Research Grant, $190,638, 1993-96. Function and Structure Determination of HIV-1 Auxiliary Proteins Syndicated R&D, $7,500,000. 1995-97. Discovery and Development of AIDS Therapeutics Based on HIV Protein Nef

7. Size and qualifications of research team supervised My last research group consisted of 39 scientists in five different disciplines, 14 with PhD degrees and 5 with MSc degrees

8. National and International Collaborations Too many to list here 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) N/A C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. I am a TWAS Research Professor at BRAC University and an Honorary Professor at Gono University. In both these institutions my role is research capacity development in Bangladesh. I am also involved in this activity as a Member of the Council of Scientific Advisers of the ICGEB (nominated by the GoB), and as a Member of the Biotechnology Standing Panel of TWAS.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? With my considerable experience in research, research management and commercialization, I am in good position to help develop Biotechnology Research in Bangladesh. I am currently helping to organize a Biotechnology Conference in Dhaka (as an Adviser and as the Conference Chair), and in preparing a Position Paper on Biotechnology Development for the GoB.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? I no longer have an active laboratory but will help young researchers in Bangladesh to develop their research programmes.

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? I am already involved in these activities and will hope to play a bigger role in future.

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? I already spend considerable time in Bangladesh (at least one month p.a. at BRAC University), and have visited Bangladesh regularly (six times in the last fourteen months). I will be prepared to spend more time in Bangladesh as Member/Chairperson the International Scientific Advisory Committee for the proposed Council for Biotechnology and the NIB.

6. Any other comments? I will leave my comments for later.

202

Dr. Ananda Kumar Saha A. Biosketch of Participants: 1. Name with academic title: Dr. Ananda Kumar Saha 2. Current position with affiliation, address and contact details: Professor, Department of Zoology,

Rajshahi University, Rajshahi 6205 Mobile: 01712637349 3. Immediate past position(s): Associate Professor 4. Graduate and postgraduate degrees and training: Graduate: Rajshahi University, Post Graduation:

University of Newcastle upon Tyne, UK. Ph.D: University of Pune, India. 5. Prizes/Awards/Honours (academic and research): Awarded second prize basing on presentation of

paper entitled “Plasmid involvement in the degradation of Trimethyl phenol by bacteria” organized by National Environmental Science Academy New Delhi, held on 25-27 October,2005 at Hyderabad, India.

6. Elected Fellowships of Academies: N/A 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: N/A

8. Membership of Institutional Committees:

Life member, The Zoological Society of Bangladesh (ZSB)

Life member, The Genetical Society of Bangladesh

Life member, National Environmental Science Academy, India

Life member, Biodiversity Research Group of Bangladesh (BRGB)

Member, Bangladesh Association for the Advancement of Science (BASS)

Member, Asiatic Society of Bangladesh

Member, Microbiological Society of Bangladesh

Member, Microbiological Society of India

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Environmental Microbiology 2. Area(s) of Expertise: Environment and Agricultural Microbiology 3. Number of peer-reviewed publications: Twenty 4. List of 5-10 most significant publications:

Chowdhury, A.H., Mossadik, M.A., Bhuyan, S.A., Rahman, M.H., Saha, A.K. and Hossain, M. 2000. Plasmid mediated degeneration of phenol by two bacterial strains, Pseudomonas sp. and Staphylococcus sp. Pakistan J. Bio. Sci. 3(6) 939-942.

Saha, A.K., Despande, M.V. and Kapadnis, B.P. 2001. Studies on survival of Rhizibium in the carriers at different temperatures using green fluroscent protein marker. Current Science. 80(5), 669-671.

Saha, A.K and Kapadnis, B.P. 2001.Effect of inoculam density on survival rate of Rhizobium carrying reporter gene gfp in different soils. Asian Jr. of Microbiol. Biotech. & Env.l Sc.3(6), 20-25.

Saha, A.K and Kapadnis, B.P. 2001 Reporter gene, gfp for monitoring survival of Rhizobium in soils. Indian J. Microbiol.3(41),153-156

Oral presenters

203

Saha, A.K. and Kapadnis, B.P. 2002. Green fluorescent protein as marker to monitor the fate of Rhizobium sp. released into the rhizosphere of Cowpea [Vigna unguiculata (L.)Walp] and Green gram [Vigna radiata (L.) Wilezeck]. Asian Jr. of Microbiol. Biotech & Env. Sc. 4(3):309-316.

Saha, A.K. and Kapadnis, B.P. 2002. Effect of adhesives on population of green fluoresence protein (gfp) marked Rhizobium on legume seeds and their germination. Bangladesh j.genet.biotechnol. 2: 133-141.

Saha, A.K. and Haque, M.F. 2002. Effect of soil quality on the viability of GFP marked Rhizobium. Univ.j.zool.Rajshahi univ. 21: 59-61.

Saha, A.K., Mohonta, M.K., Rahman, A. and Salam, M.A. 2005. Plasmid mediated degradation of carbofuran by bacteria. Bangladesh j.genet.biotechnol. 6 (1&2):29-32

Saha, A.K. and Haque, F. 2005. Effect of inoculation with Rhizobium on nodulation and growth of bean, Dolichos lablab. J.Life Earth Science. 1(1):71-74

5. Patents (PCT applications/granted) N/A 6. Competitive Research Grants obtained: UGC, University of Rajshahi, Ministry of Science and

Technology, Bangladesh 7. Size and qualifications of research team supervised: Five (M.Phil/ Ph.D.) research students are

working at present under my supervision. 8. National and International Collaborations: 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): Cooling

refrigerator centrifuge, Gel Electrophoresis apparatus, -200C cooling Incubator, Laminar Flow, Spectrophotometer etc.(Most welcome for research students to use the equipments available at our Lab.)



1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so, please provide brief information.

Microbial Degradation of Effluent from Leather Tanning Industry in Bangladesh.(Financed by Ministry of Science and Information & Communication Technology)

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Biodegradation of hazardous pollutants and promote and provide facilities of higher studies and

coordinated research on advanced biological fields in the country for M.Phil and Ph.D. degree. 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory?

Yes. 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Yes. 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions

would induce you to return? N/A 6. Any other comments?

204

Dr. Aparna Islam A. Biosketch of Participants: 1. Name with academic title:

Dr. Aparna Islam 2. Current position with affiliation, address and contact details

Assistant Professor, Biotechnology Program, Department of Mathematics and Natural Sciences, BRAC University, Dhaka. Email: [email protected] Mobile: 0187114304

3. Immediate past position(s): Post-doctoral Fellow, USDA Project, Department of Botany, University of Dhaka, Dhaka.

4. Graduate and postgraduate degrees and training BSc (Hons.): Department of Botany, DU. MSc (with thesis in ‘Plant Biotechnology’): Dept. of Botany, DU with thesis in tissue culture and Agrobacterium-mediated transformation of peanut. PhD: Plant Transformation Group, ICGEB, New Delhi in Plant Transformation and antifungal gene isolation and characterization. Post-doctoral Fellow: USDA-project, Department of Botany, University of Dhaka, Dhaka.

5. Prizes/Awards/Honours (academic and research) a. ‘Post-Doctoral Fellowship’ of the United State Department of Agriculture (USDA) from

February 2005 till January 2007. b. ‘Pre-Doctoral Fellowship’ of the International Centre for Genetic Engineering and Biotechnology

(ICGEB) from January 2001 till December 2004. c. ‘Research Fellowship’ under the agricultural project entitled ‘Application of Biotechnology in the

Improvement of Jute, Kenaf and Allied Fibres’ of International Jute Organisation (IJO) from March 1999 to December 1999.

d. ‘National Science and Technology Fellowship’ under the Ministry of Science and Technology, Government of Bangladesh from 1st November 1997 to 28th February 1998.

6. Elected Fellowships of Academies: None 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: None 8. Membership of Institutional Committees: None. But have membership of several learned society,

such as, Life Member, Bangladesh Association Plant Tissue Culture & Biotechnology (BAPTC&B). Life Member, Bangladesh Botanical Society. Member, Global Network of Bangladeshi Biotechnologist (GNOBB). Member, Asiatic Society of Bangladesh.


Plant Biotechnology including plant tissue culture and Agrobacterium-mediated genetic transformation to develop transgenic plant.

2. Area(s) of Expertise Plant Biotechnology

3. Number of peer-reviewed publications Three scientific papers and two review / opinion papers


Oral presenters

205

(ii) RESEARCH ARTICLE a. Islam A, A Hassari and VS Reddy. 2007. Analysis of Molecular and Morphological

Characteristics of Plants Transformed with Antifungal Gene. Bangladesh J. Bot. 36(1) (accepted) b. Sarker RH, MN Islam, A Islam and ZI Seraj. 2000. Agrobacterium-mediated genetic

transformation of peanut (Arachis hypogaea L.). Plant Tissue Cult. 10(2): 137-142. c. Sarker RH and A Islam. 2000. Direct organogenesis from leaflet explants of peanut (Arachis

hypogaea L.). Bangladesh J. Bot. 29(2): 109-114. d. Sarker RH and A Islam. 1999. In vitro regeneration and genetic transformation of peanut (Arachis

hypogaea L.). Dhaka Univ. J. Biol. Sci. 8(2): 1-9. (iii) REVIEW ARTICLE e. Islam A. 2006. Fungus resistant transgenic plants: strategies, progress and lessons learnt. Plant

Tissue Cult. & Biotech. 16(2): 117-138. Section 4.03 POPULAR ARTICLE

f. Islam A. 2006. GM crop: whether to accept or reject? Palindrome. 1(1): 22-23. 5. Patents (PCT applications/granted)

g. V. Siva Reddy, Afif Hassari and Aparna Islam have applied for a patent for an invention titled “New antifungal protein isolated from the seeds of chickpea, methods for isolation of such proteins, cloning of the encoding gene and transgenic plants incorporating such genes” on 31st December 2004. Indian Patent Application No. 2617/DEL/2004.

6. Competitive Research Grants obtained : None 7. Size and qualifications of research team supervised 8. National and International Collaborations 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Not involved in capacity building but capacity assessment in the “National Capacity Self-Assessment (NCSA)” project of MoEF with technical help from IUCN, as a member of the “Thematic group” on ‘Cartagena protocol on Biosafety including risk assessment and risk management’. This project aims at capacity assessment of our country in Biotechnology and Biosafety.

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? During my PhD and Postdoctoral Fellow position I have been trained in various field of Biotechnology along with Plant transformation which includes antimicrobial protein identification, purification, and characterization; toxicity analysis etc. I have also been trained at home and abroad in the field of Biosafety which is very much related to GM plant releases to the environment. Utilizing these expertises in national interest related to plant sciences project will benefit in achieving our goal to develop Bangladesh in the field of Plant Biotechnology.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? At my present institute Biotechnology lab is yet to be developed. However, if any training program is organized at any other institute then I would be very happy to work as a resource person to train young scientists and students in various field of plant biotechnology.


5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? N/A


206

Jalaluddin Bhuiyan A. Biosketch of Participants:

1. Name with academic title: Jalaluddin Bhuiyan, PhD, DABCC, FACB 2. Current position with affiliation, address and contact details: Head of Clinical Biochemistry,

Department of Pathology & Laboratory Medicine, King Faisal Specialist Hospital & Research Center, Kingdom of Saudi Arabia. MBC 10, P.O. Box 3354, Riyadh 11211 Kingdom of Saudi Arabia Tel 966 1 442 4293 (Office) Mobile 966 503207589 Fax 966 1 442 4280 E-mail: [email protected]

3. Immediate past position(s) Graduate and postgraduate degrees and training

4. Prizes/Awards/Honours (academic and research) 5. Elected Fellowships of Academies 6. Research Advisory Positions/Journal Editorship/Directorship of Boards 7. Membership of Institutional Committees

Jalaluddin Bhuiyan, PhD, DABCC, FACB Head of Clinical Biochemistry, Department of Pathology & Laboratory Medicine, King Faisal Specialist Hospital & Research Center MBC 10, P.O. Box 3354, Riyadh 11211 Kingdom of Saudi Arabia Tel 966 1 442 4293 (Office) Mobile 966 503207589 Fax 966 1 442 4280 E-mail: [email protected]

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research 2. Area(s) of Expertise 3. Number of peer-reviewed publications 4. List of 5-10 most significant publications 5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained 7. Size and qualifications of research team supervised 8. National and International Collaborations 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) For above A & B, please see my attached CV C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Very little and in early stage. Offering support in introducing ‘Laboratory Science Courses’ in the Department of Biochemistry and Molecular Biology, Dhaka University

2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? Correct diagnosis and clinical practices are closely inter-related with biotechnological innovation.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? No. Difficult to enter Saudi and Lab is mainly clinical. 4. Would you be interested in conducting and/or participating in short-term teaching and training

programmes in Bangladesh? Yes. 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? Yes. 6. Any other comments? Thanks to OC for invitation to give a talk.

Oral presenters

207

Md. Jamal Uddin

A. Biosketch of Participants: 1. Name with academic title: Md. Jamal Uddin, BSc in Animal Husbandry and MS in Dairy Science 2. Current position with affiliation, address and contact details: Scientific Officer (Dairy

Microbiology), Biotechnology Division, BLRI, Savar, Dhaka-1341. 3. Immediate past position(s): Not applicable 4. Graduate and postgraduate degrees and training: BSc in Animal Husbandry and MS in Dairy Science 5. Prizes/Awards/Honours (academic and research): Not applicable 6. Elected Fellowships of Academies: Not applicable 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Not applicable 8. Membership of Institutional Committees: Not applicable B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Comparison of the physical,

chemical and bacteriological characteristic of indigenous milk available in selected region with that of laboratory made products. To standardize the methods for the preparation of milk products.

2. Area(s) of Expertise: Dairy Microbiology 3. Number of peer-reviewed publications: 5 4. List of 5-10 most significant publications:

M.N. Sultana, A. Wadud, M.N. Islam and M.J. Uddin (2006). Study on the quality of dahi prepared from buffalo milk with the addition of different levels of soya milk. Journal of Bangladesh Agricultural University. Vol. 4(1): 111-116.

M.J. Uddin1, M.N. Hassan1, A. Wadud1 S. Basak1 and M.R. Hassan1 (2006). The manufacture of ice cream from skim milk with the addition of vegetable oil and different levels of non-fat dry milk. Bangladesh Journal of Animal Science. (Accepted)

M.J. Uddin1, M.R. Hassan, S. Ahmed and M. Asaduzzaman2 (2006). Effect of different levels of volume reduction of skim milk on the storage life of dahi prepared from cows milk. Bangladesh Journal of Bangladesh Livestock Research. (Accepted)

M.R.Hassan1, A. Wadud1, A. Akbar2, M.J. Uddin1 and M.S. Huda2(2006).Effect of partial replacement of concentrate mixture by poultry droppings on milk yield and milk composition changes of lactating crossbred cows. Bangladesh Journal of Animal Science. (Accepted)

S. Basak1, M. N. Hassan1, M.J. Uddin1, A. Iqbal2, M. N. Hasan3 (2006). Study on the quality of rasogolla prepared from cow’s milk with the addition of different levels of flour. Bangladesh Journal of Animal Science. (Accepted)

5. Patents (PCT applications/granted): Not applicable 6. Competitive Research Grants obtained: Not applicable 7. Size and qualifications of research team supervised: Not applicable 8. National and International Collaborations: Not applicable 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): Not

applicable

Oral presenters

208

Lamia Sharmeen, PhD A. Biosketch of Participants: 1. Name with academic title : Lamia Sharmeen, PhD 2. Current position with affiliation, address and contact details: Founder and Chief Scientific Officer,

Nanoderm Therapeutics Inc. 3. Immediate past position(s): Associate Research Fellow & Group Leader, Molecular Science and

Technology, Pfizer Global R&D Inc. 4. Graduate and postgraduate degrees and training: Graduate training in Cell Biology & Biochemistry,

Department of Biochemistry, University of Sydney, Australia. Thesis Title: Role of cyclic AMP in B16 melanoma cells. a. Post doctoral training in Animal Virology at Fox Chase Cancer Center, Philadelphia, USA with

Dr. John Taylor b. Senior Fellow in department of molecular medicine, Fred Hutchinson Cancer c. Center, Basic Science Division.

5. Prizes/Awards/Honours (academic and research) : (a) Dhaka University Salekunnesa Gold Medal (b) University of Sydney Post-graduate research scholarship (3) NIH individual fellowship grant (4) American Cancer Society post-doctoral grant (5) University of Washington Virology training grant

6. Elected Fellowships of Academies N/A 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: University of Michigan

Technology Transfer (consulting advisor), ad hoc reviewer for Antiviral Research, Journal of Virology/ Biosafety level 1-3 laboratory management board (Parke-Davis/Warner Lambert), Co-director of Biotech Start-up Company.

8. Membership of Institutional Committees: Biosafety committee, Ann Arbor site Pfizer, Investigational New Drug (IND) application committee Parke Davis and Pfizer, Ann Arbor site.

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Signal transduction through G-

protein coupled receptor proteins, small peptides, Retrovirology, Neurodegeneration & small RNA biology.

2. Area(s) of Expertise: Anti-infective, Antiviral, Central Nervous system Diseases and wound care. 3. Number of peer-reviewed publications ~ 20 4. List of 5-10 most significant publications

i. HIV-1 Tat induces neuronal cell death through activation of NMDA receptors. Aleida Perez, Albert W. Pobert, Kevin K.W. Wang and Lamia Sharmeen, J.of Neurovirology, 2001, 7( 2 ), 1-10.

ii. Inhibition of the early phase of HIV replication cycle by an isothiazolone, PD161374 (CI-1012). Lamia Sharmeen, Thomas McQuade, Andrea Heldsinger,Rocco Gogliotti, John Domagala and Stephen Gracheck. Antiviral Research, 2001, 49,101-114.

iii. Sharmeen, L., Adams, M., Kimpton, J., Romeo, J., Garcia, J. V., Peterlin, B. M., Groudine, M, and Emerman, M.( 1994 ) Cellular latency in human immunodeficiency virus-infected

Oral presenters

209

individuals with high CD4 levels can be detected by the presence of promoter-proximal transcripts. Proc. Natl. Acad. Sci.( USA ), 91, 3862-3866.

iv. Sharmeen, L., Bass, B., Sonenberg, N., Weintraub, H., and Groudine, M. ( 1991 ) Tat dependent adenosine to inosine modification of wild-type TAR RNA. Proc. Natl. Acad. Sci. USA, 88, 8096-8100.

v. Kuo, M.Y.-P., Sharmeen, L., Dinter-Gottlieb, G. and Taylor, J. (1989 ) Characterization of self cleaving RNA sequneces on the genome and antigenome of human hepatitis delta virus. J. Virol. 62, 4439-4444.

5. Patents (PCT applications/granted) : 1. Combination of Atomoxetine and a 5HT1a receptor agonist for treating ADHD and other disorders Inventors / Carroll, RT & Sharmeen, L. WO 2005/115396 A2.

6. Competitive Research Grants obtained: National Institute of Health (NIH), University technology commercialization grant, small business development grant.

7. Size and qualifications of research team supervised: 3-10 Masters to PhD levels. 8. National and International Collaborations: US academic and corporate research projects. 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) N/A C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. No. 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? My

background and expertise can help develop drug discovery and commercialization of Life science technologies to benefit health, health related education and training , establish research institute through public (government) and private initiatives to develop talent pools of future scientists, educators and scientific entrepreneurs in a knowledge based society.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? May be possible.


5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? I may consider such opportunities in next few years. Conditions that can give an opportunity to utilize my expertise and add value to creating institutions of International standard in Bangladesh with a goal to produce future generation of professional, ethical, knowledgeable biotechnologists who will contribute to medical and environment sectors.

6. Any other comments? I wish and pray for success of the “promotion of biotechnology initiative” in Bangladesh in near future.

210

Khan Shahidul Huque Article V.

Name with academic title Khan Shahidul Huque, PhD (UK) Current position with affiliation, address and contact details

Chief Scientific Officer, Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, BANGLADESH Fax: 880-2-7708325, e-mail: [email protected], Tel: 880-2-7708005 (Work), 880-2-7708117 (Residence), 0152376788 (Cell)

Immediate past position(s) Principal Scientific Officer Graduate and postgraduate degrees and training

B.Sc.AH (Hons), M.Sc (Animal Nutrition), PhD Local and foreign training on statistics, Development management of animal agriculture

Prizes/Awards/Honours (academic and research)

-

Elected Fellowships of Academies - Research Advisory Positions/Journal Editorship/Directorship of Boards

Editor, Bangladesh Journal of Livestock Research, Member, Board of Management, BLRI; Technical Expert and Coordinator: Drafting group of the country report on Animal Genetic Resources in Bangladesh, Ministry Fisheries and Livestock

Membership of Institutional Committees

Member Secretary: Animal Biotechnology Action plan, Member: Drafting committee on National Guidelines for Fish and Animal Biotechnology, Ministry Fisheries and Livestock Member Secretary: National Livestock Team for Livestock Development Planning, Ministry of Fisheries and Livestock Member: National Consultative Committee for Animal genetic Resources in Bangladesh, Ministry Fisheries and Livestock Country Representative, AD HOC INTERGOVERNMENTAL CODEX TASK FORCE ON ANIMAL FEEDING; Member: Drafting Group for codex formulation on ON-FARM PRODUCTION AND USE OF FEEDINGSTUFFS under Section 6 of the FAO/WHO draft document of the UNO. Member Secretary: National Task Force on Poverty alleviation through Goat production

Major project area(s); with very brief description of current research

Development of herbal additives for feeding ruminants, Cattle fattening development, Fodder conservation and development Project Director: Red Chittagong Cattle development and conservation project

Area(s) of Expertise Dairy and beef cattle feeding and nutrition

Oral presenters

211

Number of peer-reviewed publications

36 (Thirty six)

List of 5-10 most significant publications

Huque, K.,S., Chowdhury, S.A. (1997). Study on the supplementing effects or feeding systems of molasses and urea on methane and microbial nitrogen production in the rumen and growth performances of bulls fed a straw diet. Asian Austral-Asian Journal of Animal Science, 10:35-46.

Huque, K.,S., M. M. Rahman and M. A. Jalil, 2001. Study on growth pattern of Gayals (Bos frontalis) and their crossbred calves. Asian-Aust. J. Anim. Sci. 14(9).

Huque, K.,S., M. M. Rahman and A. I. Talukder 2001. Study on forage crop production on sloping land in Bangladesh. Asian-Aust. J. Anim. Sci. 14:956-959.

Huque, K.,S., Chowdhury, S.A. and Kibria, S.S. 1995. Study on the potentiality of duckweed as a feed for cattle, Asian Austral-Asian Journal of Animal Science, 9:133-137.

Huque, K.,S., and Talukder, A. I. 1995. Effect of molasses supplementation of a roughage based diet on the growth performances of cattle. Asian-Australasian Journal of Animal Sciences, 8:337-342.

Huque, K.,S., Chowdhury, S.A., Khatun, M. and Nahar, Q. 1994. Comparative study on the effect of algae (Chlorella and Scenedemus) or oil cake supplementation of a straw diet on the rumen environment of cattle. Livestock research for Rural Development, an electronic journal, Published in Vol. 6.

Patents (PCT applications/granted) - Competitive Research Grants obtained

-

Size and qualifications of research team supervised

8 (Eight)

National and International Collaborations

Collaboration with Chittagong Veterinary and Animal Sciences University,

Major Equipment/Facilities in Bangladesh (will these be available to other researchers?)

Nutrition: Atomic Absorption Spectrophotometer, High Power Liquid Chromatography, Gas Chromatography etc Genetics and Breeding: PCR, DNA Synthesizer, DNA Concentrator, Electrophoreses etc Yes, it may be available

212

Dr. Md. Shamsul Haque Prodhan A. Biosketch 1. Name: Dr. Md. Shamsul Haque Prodhan 2. Current position with affiliation

Visiting researcher in the University of Tsukuba, Tsukuba city, Japan (Collaborative research with Ibaraki Prefectural University of Health Science) E. mail: [email protected]

3. Immediate past position: Researcher (post doctoral) Laboratory of Plant Genetic Engineering Institute of life and environmental sciences University of Tsukuba, Japan.

4. Graduate and post graduate degrees and training

Years attended University/ institution from to

Degree Awarded with class

Field of Specialization

Tokyo University of Agriculture Tokyo, Japan

April 2000

March 2003 (3 years)

Doctor of Philosophy (Agriculture with grade A)

Plant biotechnology Tissue culture and transformation with genetic engineering


April 1998 March 2000 (2 years)

Master of Science (Agriculture) with first class

Studies on tissue culture Efficient callus induction and regeneration

Bidhan Chandra Krishi ViswaVidyalaya (University) Nadia, W.B., India

October 1991

December 1995 (4 years)

Bachelor of Science (Agriculture) with first class

Agricultural science Genetics. horticulture, agronomy, soil science, Extension etc.


October 1997

March 1998 (6months)

Research student

Learning and training on Molecular techniques

Indonesian Embassy of Japan, Tokyo

February 1998

1 day Panel discussion on Indonesia’s Bio-resource Management and Policies

Tokyo University of Agriculture and Technology Tokyo, Japan

March 1997

September 1997 (6 months)

Japanese course (training)

Japanese language Intensive course for 6 months.

Oral presenters

213

5. Prizes/Merit awards/Honours/ fellowship

Best Cadet Award (Bangadesh)

From Pabna Cadet College, Bangladesh (for academic and other curriculum excellence), 1990

ICCR Award (India)

Indian Council for Cultural Relations, scholarship award, by Indian Government Ministry of Education for 4 years undergraduate program, in BCKVV, W.B., India, 1991-1995

Monbusho Award (Japan)

This award is given by the Ministry of Education, Japan for conducting the research as research student and Masters and Ph. D. program in Tokyo University of Agriculture from 1997 to 2003.

6. Membership of Institutional Committees

i) Japanese society of Biotechnology. ii) Bangladesh society of Biotechnology iii) Breeding society of Japan

B. Research and Research Management Experience 1. Major project area(s); with very brief description of current research

Research Summary:

Rice (Oryza sativa L.) is one of the most important food crops in the world and it is the staple food of Bangladesh. Salinity is a major constraint to food production because it limits crop yield. So the rice plants have to develop unique mechanisms to avoid these environmental stresses like salinity. Salinity causes hyper osmotic stress as well as oxidative stress by the production of O2

- (super oxide anion), OH- (hydroxyl radical) and H2O2 (hydrogen peroxide). Catalase acts as a scavenger to break down of H2O2 producing H2O which brings the osmotic balance and results in protecting the cells from oxidative damage to help the survival of the plants. In my previous research in Ph.D, I tried to over-express catalase by introducing catalase katE gene derived from E. coli to an Indica rice genome using the tools of genetic engineering. This transgenic rice could survive 100 mM salt concentration for more than three months and could form seeds. By the application of multi genes following the same strategy more stronger transgenic rice could be obtained.


Biotechnology/ Plant Tissue Culture/ Molecular Biology) Tissue culture technique (invitro technique) in all plants

(i) Efficient cell culture technique Callus induction and Efficient plant regeneration Embryo manipulation and transfer Micropropagation for ornamental plants Agriculture and Agribusiness development etc. ii) Genetic engineering for crops development : Transformation with reporter genes Transformation with desire gene To obtain disease and pest resistant plants Stress resistant plants by genetic engineering Production of diversified plants. iii) Molecular techniques known DNA, RNA isolation, PCR, Electrophoresis, Southern and western hybridization, Molecular marker development (SSR, AFLP etc), Gene cloning, Recombinant DNA techniques etc.

3. List of significant publications

i) Prodhan Shamsul H. (1998). Argiculture and Agribusiness development in Bangladesh. In the proceedings of International Seminar on Development of Agribusiness and its impact on Agriclutural production in South East Asia. DABIA III, p 411-419, TUA, Tokyo Japan.

214

ii) Prodhan, Shamsul H., Nagamiya, K., Komamine, A. and Hirai, Y. (2001). Regeneration Response of Indica and Japonica Rice in Different Media, Bangladesh Journal of Breeding and Genetics ,14(2) :01-06. iii) Prodhan, Shamsul H. and Hirai, Y. (2002). Growth Pattern and Yield Component Traits of Bangladesh Rice Varieties with Good Grain Quality and Salinity Tolerance tested in Japan. Jour. of Agri. Sci., Tokyo Univ. of Agric., 47 (2): 130-136, 2002 iv) Nagamiya, K., Nakao, K., Prodhan, Shamsul H., Motohashi, T., Morishima, H., Hirose, S.,

Ozawa, K., Ohkawa, Y., Takabe, T., Takabe, T. and Komamine, A. (2003). Production of salt tolerant rice by introduction of a gene encoding catalase, katE. In Plant Biotechnology 2002 and Beyond (Ed. Vasil K.), Kluwer Academic Publishers, The Netherlands, 167-170. v) Prodhan, Shamsul H., Komamine A., (2004). Recalcitrant Indica Rice Callus Initiation and

Regeneration Variability with the Effect of Plant Growth Regulators, Mol Biol Biotech J, 2 (1&2) vi) Prodhan, Shamsul H., Mannan A., Komamine A., (2006). Expression of Reporter Genes in Transformed Indica Rice Through Agrobacterium Mediated Method; Plant Tissue Culture & Biotechnology. (Accepted) vii) Prodhan, Shamsul H., Komamine A., Morishima H., (2006). Transformation of Aromatic Indica Rice (Kataribhog) Through Genetic Engineering; Bangladesh Journal of Breeding and Genetics. (Accepted, in press) Manuscripts under review i) Nagamiya, K., Nakao, K., Prodhan, Shamsul H., Shimura, K., Motohashi, T., Morishima, H., Hirose, S., Ozawa, K., Ohkawa, Y., Takabe, T., Takabe, T. and Komamine, A. Over expression

of catalase gene (katE) improved salt tolerance in rice. Plant Cell Report. (Submitted) ii Jesmin, S., Maeda, S., Mowa, N C., Zaedi, S., Togashi, T., Prodhan, Shamsul H., Yamaguchi, T., Yoshioka, M., Sakuma, I., Gando, S., Miyauchi, T., (2007). Antagonism of Endothelin Action Normalizes Altered levels of VEGF and its Signaling in the Brain of Stroke-prone Spontaneously Hypertensive Rat. European Journal of Physiology. Manuscripts in revision i) Jesmin, S., Zaedi, S., Mowa, N. C., Togashi, T., Prodhan, Shamsul H., Shimojo, N., Iemitsu, M., Yamaguchi, N., Sakuma, I., Yamaguchi, N., Miyauchi, T., Hattori, Y., Maeda, S., (2007). VEGF signaling is disrupted in the hearts of mice lacking estrogen receptor; Cardiovascular Research (preferred journal to be submitted) Manuscripts in preparation i) Prodhan, Shamsul H., Nagamiya K., Jesmin S., Komamine A., Morishima H., (2007). Catalase Gene (katE) Overxpression and Improved Salt Tolerance in Indica rice; Plant Science, (preferred journal to be submitted). ii) Prodhan, Shamsul H., Komamine A., Morishima H., Fujimura T., (2007). Development of molecular markers for the construction of genome map in sweetpotato; Theory and Applied Genetics, (preferred journal to be submitted) iii) Prodhan Shamsul H., Nagamiya K., Jesmin S., Komamine A., Morishima H.,(2007). Improved Salt Tolerance and morphological Variation in Transgenic Indica rice derived from E. coli; African Journal of Biotechnology; (preferred journal to be submitted)

Presentations at Symposium or Meeting of academic society i) Prodhan, Shamsul H. (1998). Argiculture and Agribusiness development in Bangladesh. Presented at International Seminar on Development of Agribusiness and its impact on Agriclutural

production in South East Asia, held at Tokyo University of Agriculture, Tokyo, Japan. ii) Prodhan, Shamsul H., Komamine, A. and Hirai, Y., (2000). Differentiation of rice with special reference of indica (IR-36) and Japonica (Nipponbare) rice response on different induction and regeneration medium. In the proceedings of the 2000 Japan Korea Joint Symposium of plant science, p172, held at July 22-24, University of Shizuoka, Shizuoka Japan. iii) Prodhan, Shamsul. H., Asada, M., Motohashi, T., Komamine, A. and Morishima, H., (2002). Agrobacteriummediated transformation of Indica rice plants. Breeding Research, Vol. 4 (Special issue 1):167, (101st Meeting of Japanese Society of Breeding), Tokyo, Japan.

215

iv) Komamine, A., Nagamiya, K., Nakao K., Prodhan, Shamsul H., Motohashi, T., Ohkawa, Y., Hirose, S., Ozawa, K., Takabe, T. and Takabe, T. (2002). (Oral presentation by Komamine, A.) Production of salt tolerant rice by introduction of a gene encoding catalase, katE.10th International Association Plant Tissue Culture and biotechnology Congress, Orlando, Florida, USA. v) Prodhan, Shamsul H., Shishido, T., Asada, M., Motohashi, T., Komamine, A. and Morishima, H. (2002). Production of salt tolerant Indica rice harboring katE gene through Agrobacterium mediated transformation. Breeding Research, Vol. 4(Special issue 2): 198, (102nd Meeting of Japanese Society of Breeding, Obihiro, Japan vi) Prodhan, Shamsul H., Asada, M., Motohashi, T., Komamine, A. and Morishima, H., (2003). Production of salt tolerant Indica rice harboring katE gene through genetic engineering, Oral presentation in the seminar of Bangladesh Sugarcane Research Institute (BSRI), Ishurdi, Pabna, Bangladesh.

4. Competitive Research Grants obtained (with project title) In the year 2000, Studies on the tissue culture of Indica rice with special reference to good callus induction and regeneration medium; Tokyo University of Agriculture, Japan

In the year 2003, Production of Salt Tolerant Indica Rice Harboring katE, a Gene Encoding Catalase, Through Agrobacterium Mediated Transformation; Tokyo University of Agriculture, Japan. In the year 2004, Development of molecular marker and construction of genome map in sweetpotato

5. Size and qualifications of research team supervised Three undergraduate students and one Masters students research were supervised partially with the host professor 6. International collaborative research

Collaborative research with Ibaraki Prefectural University of Health Science, Ami city, Ibaraki prefecture, Japan C. Capacity Development and Training Activities in Bangladesh 1. I am not yet involved in any collaborative research in Bangladesh. But I visited several times in

Bangladesh Sugarcane Research Institute (BSRI) and Bangladesh Rice Research Institute (BRRI) laboratory of Biotechnology. We had some discussions regarding the collaborative research with Japan. My host professor (Dr. A. Komamine, Director of Research Institute of Evolutionary Biology and Kihara Institute) has great will and desire to start the collaborative research with some institutions of Bangladesh. I hope I shall be able to start the program in near future.

2. My last 9 years experience of research in Biotechnology, if I get opportunity in Bangladesh I shall try to apply and teach the young scientists for the benefit of my country.

3. During my research period in Japan I had opportunity to work and teach with many Japanese and other foreign students. From these experiences I hope I would be able to train Bangladeshi students and researchers.

4. I am interested (on the basis of time schedule) to conduct and/ or participating in short term teaching and training programmes in Bangladesh.

5. Bangladesh is my motherland and from my core of heart I have wish and desire to serve the country. But all the people know the reality, to enter any person to any place/ institution how the authority evaluate. Now, we hope the situation will be changed and in future according to merit basis all the recruitment will be done and government will create more facilities with modern equipments and institution.

216

Dr. Md. Ekramul Hoque A. Biosketch of Participants: 1. Name with academic title Dr. Md. Ekramul Hoque Ph.D in Molecular Genetics Post-Doc. (Genetic Engineering), ICGEB 2. Current position with affiliation, address and contact details Assistant Professor and Chairman Dept. of Biotechnology Sher-e-Bangla Agriculture University Sher-e-Bangla Nagor, Dhaka-1207 Mobile : 01712-836595 Tel : 0088-02-9144270-8 ext-284 E-mail : [email protected] 3. Immediate past position(s) Senior Scientific Officer Biotechnology Division Bangladesh Agricultural Research Institute (BARI) Gazipur-1701 4. Graduate and postgraduate degrees and training Graduate degree B. Sc. Ag (Hons.) Bangladesh Agricultural University, Mymensingh Post-graduate degree MS (Genetics and Plant Breeding) Institute of Post-graduate studies in Agriculture (IPSA) Gazipur-1703 Training Post-Doctoral training at International Centre for Genetic Engineering and Biotechnology (ICGEB) New Delhi, India 5. Prizes/Awards/Honours (academic and research) Not applicable 6. Elected Fellowships of Academies Not Applicable 7. Research Advisory Positions/Journal Editorship/Directorship of Boards Research Advisory Positions Research supervisor of following Department

Biotechnology Biochemistry Genetics and Plant Breeding Horticulture

Journal Reviewers International Journal of Sustainable Agricultural Technology (IJSAT), Dhaka. Bangladesh Journal of Agricultural Research, BARI, Gazipur Journal of Subtropical Agricultural Research and Development (JSARD), Dhaka. Journal of Agricultural Education and Technology (JAET), SAU, Dhaka.

8. Membership of Institutional Committees Not applicable

Poster presenters

217

B. Research and Research Management Experience: 1. Major project area(s): with very brief description of current research Project area(s)

DNA fingerprinting and molecular diversity analysis Gene cloning, sequencing and expression Morphological characterization of Agricultural crops. All the above project were submitted in different government and international organization.

2. Area(s) of Expertise Gene tagging and mapping Marker Assisted Selection (MAS) DNA fingerprinting and polymorphism study Gene cloning, sequencing and expression Protein purification and characterization Plant Tissue Culture

3. Number of peer-reviewed publication Full length paper- 15 Short communication- 1 Abstract- 3

4. List of 5 – 10 most significant publication Hoque M.E., A. Bhowmik and M. Kalequzzaman. 1998. In vitro culture of pointed gourd (Trichosanthes dioica Roxb.). Thai J. Agric. Sci. 31(3): 369-374. Hoque M.E., S.K. Mishra and A. Sarkar, 2002. Inheritance and linkage relationship between morphological and RAPD markers in lentil (Lens culinaris Medik.). India J. Genet., 62(1): 5-10. Hoque M.E., S.K. Mishra., Yogesh Kumar., Rajesh Kumar and S.M.S. Tomar. 2002 Inheritance of leaf colour and plant pubescence and their linkage in lentil (Lens culinaris (Medik.). Indian J. Genet., 62(2): 140-142. Hoque, M.E., M.T. Islam and M.A.K Main, 2002 Sex modification in pointed gourd (Trichosanthes dioica Roxb.). Indian J. Hort., 59(1): 52-56. Islam M.T., M.A.K. Mian. M.E. Hoque and M. A. Afzal. 1998. Inheritance of photoperiod sensitivity in Mung bean (Vigna radiata L.Wilczek). Thai J. Agric. Sci., 31(2): 217-223. Hoque, M. E., M. S. Hossain and S. K. Mishra. 2007. Digenic inheritance of cotyledon colour and its epistatic interaction in lentil (Lens culinaris Medik.). Int. J. Sustain. Agril. Tech. 3(1): 07 – 11, February, 2007. (An online journal of “g-science: Implementation and publication”) Hoque M.E., S.K. Mishra., Y.Kumar, R.Kumar and S.M.S. Tomar. 2002. Inheritance and linkage of flower colour , testa pattern and testa colour in lentil (Lens culinaris Medik.). Bangladesh J . Pt Breed., 15(2):01-08. Kumar, Yoges., S. P. Singh, S. K. Mishra, Shrinivas Giri and M. E. Hoque. 2005. Arrangement of four genes in the linkages group I of lentil. Bangladesh J. Agril. Res. 30 (4): 615-621. Abstract Hoque, M.E., S.Sivakumer, Rajagopal R and R.K. Bhatnagar. 2004. Cloning and characterization of prophenol oxidase activating enzyme. The 5th International Plant Tissue Culture & Biotechnology Conference. Organised by Bangladesh Association or Plant Tissue Culture & Biotechnology (BAPTC&B). December 4-6, 2004, Dhaka, Bangladesh. Hoque, M.E., M.T. Islam and M.A.K. Mian. 2001. Sex modification in pointed gourd (Trichosanthes dioica Roxb.). Diamond Jubilee Symposium of the Indian Journal of Genetics and Plant Breeding. Organised by The Indian Society of Genetics and Plant Breeding, November 6-9, 2001, New Delhi, India.

5. Patents (PCT applications/granted) Gene Bank Registration

(1) Cloning and sequencing of prophenol oxidase activating enzyme 1 (PPAE 1)

218

in Spodoptera litura AY677081 (Available on 1st August, 2005) (2) Cloning and sequencing of prophenol oxidase activating enzyme 3 (PPAE 3) in Spodoptera litura AY677082 (Available on 1st August, 2005)

6. Competitive Research Grants obtained Not applicable 7. Size and qualification of research team supervised M.S Student from three Department 8. National and International Collaborations National

Bangladesh Agricultural Research Institute (BARI) Bangladesh Rice Research Institute (BRRI) Bangabandhu Sheikh Mujibur Rahaman Agricultural University (BSMRAU) Bangladesh Agricultural University (BAU)

International Trying to collaboration with International Centre for Genetic Engineering and Biotechnology (ICGEB), Italy International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) Biotechnology is a new Department at Sher-e-Bangla Agricultural University, Dhaka. At present only the minor equipments are available. We are trying to establish a modern molecular biology lab. Collaboration and donation is needed from different National and International Organization.

C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientist) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Not Applicable

2. How can you research, expertise and resources benefit Biotechnology research in Bangladesh? Not Applicable 3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Yes, I can provide research training to Bangladesh students and young researchers. But as a new

Department the infra-structure of my lab is not suitable. Need support for establishing molecular lab at Sher-e-Bangla Agricultural University.

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh?

Yes, I am interested 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? Not applicable 6. Any other comments? Not applicable

219

Halima Sadia Khan A. Biosketch of Participants:

1. Name with academic title: Halima Sadia Khan (Ph.D) 2. Current position with affiliation, address and contact details:

a. Current position and address: Lecturer of Biology, Al-Hajj Mockbul Hossain University College, Kaderabad Housing, Mohammadpur, Dhaka-1207 b. Affiliation: Life member, Genetical Society of Bangladesh c. Contact: E-mail: [email protected], [email protected]

Phone: 0152328633 3. Immediate past position(s): Not applicable 4. Graduate and postgraduate degrees and training: B.Sc., M.Sc. (Zoology, Sp. branch: Animal Genetics

and Breeding) 5. Prizes/Awards/Honours (academic and research): Awarded fellowship under N.S.I.C.T.F program

during Ph.D 6. Elected Fellowships of Academies: Not applicable 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Not applicable 8. Membership of Institutional Committees: Not applicable B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research:

Project area- Genetic manipulation of reproductive potential in the housefly Musca domestica L. (Diptera:Muscidae); Description- Using gamma radiation doses of 0-10 Gy to pupal and adult stadium, reproduction and survival parameters in the parents and subsequent progenies up to F3 generations of the common houseflies Musca domestica L. have been investigated. For the pupal treatment, compared to the untreated controls, irradiations significantly reduced egg laying, increased sterility and immature mortality, and diminished adult eclosion and female ratio in the treated lines. Sterility reached 100% above 5 Gy levels except for the cross U×I that resulted in 55.2±21.3% sterility at 6 Gy, while complete infecundity in all flies was induced by 10 Gy. Males were readily radiosterilized than the females. Dose mortality (LD50) and sterility (SD50) responses of the test insects were determined 53.03 Gy and 4.34 Gy, respectively. Otherwise in the adult treatment, compared to the untreated controls, irradiations significantly increased at all the parameters with oviposition in the treated lines. Sterility reached 100% above 8 Gy levels and males were also readily radiosterilized than the females. LD50 and SD50 responses of the test insects were determined to be 10.04 Gy and 6.23 Gy, respectively. The findings of the investigation showed that gamma radiation had also much effect on longevity and salivary gland size (decreased as the dose increased) of M. domestica and showed maximum percentage of dominant lethal mutations than UV radiation. Results are promising in connection with a sterile insect technique (SIT) for this pest species.

2. Area(s) of Expertise: Animal Genetics and Breeding [Title of Ph.D research: Genetic manipulation of reproductive potential in the housefly Musca domestica L. (Diptera:Muscidae)]

3. Number of peer-reviewed publications: ------------- 4. List of 5-10 most significant publications:

Published: Islam, M. S. & Khan, H. S. 2000. Changes in reproductive attributes associated with larval rearing density in the housefly, M. domestica L. Univ. J. Rajshahi Univ. 19: 61-66.

Poster presenters

220

Khan, H. S. & Islam, M. S. 2005. Effect of larval rearing density on the heritability of reproductive potential in the housefly, Musca domestica L., Bangladesh j. genet. biotechnol. 6 (1&2): 69-70. Khan, H. S., Islam, M. S. & Salam, M. A. 2005. Gamma radiation-induced deformities in the F1 progenies of housefly Musca domestica L. (Diptera: Muscidae) Bangladesh j. genet. biotechnol. 6 (1&2): 71-73.

Accepted: Khan, H. S., Islam, M. S. & Salam, M. A. The effects of UV and gamma radiations on adult recovery and adult mortality in Musca domestica L. (Diptera: Muscidae). J. Asiat. Soc. Bangladesh (Serial No. S1143) Islam, M. S. & Khan, H. S. Efficacy of gamma radiations against housefly (Musca domestica L.) reproduction and survival I. Pupal treatment. J. Faculty of Bio. Sci. Khan, H. S. & Islam, M. S. Efficacy of gamma radiations against housefly (Musca domestica L.) reproduction and survival II. Adult treatment. J. Bio. Sci.

Submitted: Khan, H. S., Salam, M. A. & Islam, M. S. The effects of radiations on dose-mortality and dose-sterility responses in Musca domestica. Bangladesh J. Entomol.

5. Patents (PCT applications/granted): Not applicable 6. Competitive Research Grants obtained: National Science and Information & Communication

Technology Fellowship 7. Size and qualifications of research team supervised: Not applicable 8. National and International Collaborations: Not applicable 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): Not

applicable C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists) 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. Not applicable 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh?

The results of my research suggest that the reproductive attributes of the progeny of irradiated pupae of M. domestica be affected by the gamma radiation in a proper manner and carry-over effects in generation to generation. It could be said that the results of these efforts would be helpful to control of this noxious species through Sterile Insect Release Method. We can also use this technique against mosquito and any other pest species.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? Not applicable

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh? Yes

5. Would you consider returning to Bangladesh or spending substantial time there? If so, what conditions would induce you to return? Not applicable

6. Any other comments? a. I think we will get a lot of information about current research on biotechnology in whole world

from this International Conference. b. This conference will play an important role to create interaction among scientists, researchers and

others who think progress for our country and science. c. We could exchange our views from several corners of bioscience d. We could share our ideas in several ways to apply biotechnology in our country according to

proper manner e. I think this conference will influential to develop of biotechnology in Bangladesh.

221

Imran Parvez A. Biosketch of Participants: 1. Name with academic title Imran Parvez Lecturer 2. Current position with affiliation, address and contact details Lecturer

Department of Fisheries Biology & Genetics Hajee Mohammad Danesh Science and Technology University Dinajpur-5200 Email: [email protected] Mobile: +8801713-163344

3. Immediate past position(s) Student M.S. in Biotechnology Biotechnology Department Bangladesh Agricultural University Mymensingh-2202

4. Graduate and postgraduate degrees and training Academic Career:

Name of Examination

Name of Institution

Board/ University

Year of Passing

Marks obtained

(%)

Division/ Class

S.S.C. Ishwargong Bisweswari pilot

high school. Dhaka 1995 84.3%

1st

Division

H.S.C. Notre Dame College Dhaka 1997 69.2% 1st

Division.

B.Sc. Fisheries (Hons.)

Bangladesh Agricultural University

Mymensingh


Mymensingh.

2002(Held in 2004) 68.83% 1st Class.

Master of Science in

Biotechnology


Mymensingh


Mymensingh.

December 2005

GPA 3.791 (out of 4) A grade

Training:

No. Name of the Course Institute Duration 1. Training Course on

“Aquaculture and Extension”

Mymensingh Aquaculture and Extension Project (MAEP), Mymensingh

6 days

2. Training Course on Fisheries and Aquaculture Research

Bangladesh Fisheries Research Institute (BFRI), Mymensingh

6 days

3. Extension Field Trip Department of Agricultural Extension and Education at Bhaluka, Mymensingh

7 days

Poster presenters

222

5. Prizes/Awards/Honours (academic and research) Fellowship of Bangladesh Agricultural University Research System under the supervision of Dr. Md.

Mukhlesur Rahman Khan, Head, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University

6. Elected Fellowships of Academics Junior scholarship (1992), S.S.C scholarship from Dhaka Board 7. Research Advisory Positions/Journal Editorship/Directorship of Boards None 8. Membership of Institutional Committees Bangladesh Fisheries Research Forum (BFRF), Bangladesh (2006) B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research:

Research work to know the Effect of 17 α-methyl testosterone on growth and sex-ratio of common carp, Cyprinus carpio var. communis were preliminary conducted and more research work relating to this also going on for higher production of common carp. We know that in common carp production there is the major problem that the early maturation of the female. This study is conducting due overcome the problems of early maturation.

2. Number of peer-reviewed publications: 3. List of 5-10 most significant publications: Full Length Paper

1) Parvez I., M.M.R.Khan, S.M.Zakiur Rahman and M .A.Alam. (2006). Present status and future potential of the gene pool of local sarpunti, Puntius sarana (Hamilton), J of Bangladesh Agril. Univ. 4(2): 319-324

2) Alam, M.A., I., Parvez and M.M.R.Khan. (2006). Effect of 17 α-methyl testosterone on growth and sex-ratio of common carp, Cyprinus carpio var. communis (Linnaeus). J of Bangladesh Agril. Univ. 4(2): 313-318

3) Parvez I. and M. M. R. Khan (2005). Effect of feed on larval rearing of endangered local sarpunti (Puntius sarana, Hamilton) in laboratory condition. Bangladesh J. Fish, 29 (1&2): 63-68.

4) Khan M.M.R., M.S. Alam, S.M.Z. Rahman and I. Parvez (2003). Genetic variation of Tilapia strains inferred by allozyme marker. Journal of Molecular Biology and Biotechnology Journal, 1(1&2): 59-62.

Short Length Paper (Proceedings) 1) Noor, A.M., M.M.R. Khan, S.M.Z. Rahman and I. Parvez. 2006. Growth and morphological

comparison between local and Thai koi Anabas testudinius (Bloch) in Bangladesh. 2nd Fisheries Conference and Research Fair 2006, BFRF, Abstracts. p 6.

2) Rahman, S.M.Z., M.M.R. Khan, M.S. Islam, I. Parvez and M.S. Alam. 2006. Genetic variation study in wild and hatchery population of Catla catla (Ham.) using randomly amplified polymorphic DNA (RAPD) markers. 2nd Fisheries Conference and Research Fair 2006, BFRF, Abstracts. p 11-12.

3) Parvez, I., M.M.R. Khan and S.M.Z. Rahman. 2006. Study for conservation of endangered local sarpunti Puntius sarana (Ham). 2nd Fisheries Conference and Research Fair 2006, BFRF, Abstracts. p 13-14.

4) Khan, M. M. R. and I. Parvez (2005). Comparison of genetic variation of local sarpunti (Puntius sarana) and Thai sarpunti (Barbodes gonionotus) using allozyme marker. Proceeding Bangladesh Agricultural University Research Progress. Workshop held on dated 15-16 January 2005, Vol. 15. pp 75.

223

4. Patents (PCT applications/granted): Fry rearing of endangered local sarpunti Puntius sarana in laboratory condition, development of mono-sex male and sterile common carp production technology to over come the problems of early maturation of the species

5. Competitive Research Grants obtained: None 6. National and International Collaborations: None 7. Size and qualifications of research team supervised: None 8. National and International Collaborations: None 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?) To conduct such type of study relating to fish genetic variation and selective breeding for the genetic

improvement of fish species specially the endangered one as far I know facilities are available in the laboratory of Department of Fisheries Biology and Genetics and also the central laboratory of Bangladesh Agricultural University, Mymensingh

C. Capacity Development and Training Activities in Bangladesh: (Information requested from Expatriate Scientists)- 1. Are you currently involved in any collaboration or capacity building activities in Bangladesh? If so,

please provide brief information. NO 2. How can your research, expertise and resources benefit Biotechnology research in Bangladesh? So far, I have conducted research work as a young man; I think my research topic can benefit

biotechnology research by genetic improvement of fish species. This genetic improvement is concerned with increasing the genetic variation of the species and by achieving the genetic variation we can get individuals with higher genetic variability’s which ultimately increases the growth and survival of the species. This genetic improvement can only be achieved first by collection of fish species from different location, and then make their genetic evaluation as well as morphological evaluation and then select the quality brood for production of spawn. And I think the spawn which will produce by this method will perform higher growth and survival rate. Thus can enhance our fish production and also can save our fish species to become extinct.

3. Can you provide research training to Bangladeshi students and young researchers in your laboratory? No

4. Would you be interested in conducting and/or participating in short-term teaching and training programmes in Bangladesh?

Yes I need training relating to biotechnology 5. Would you consider returning to Bangladesh or spending substantial time there? If so, what

conditions would induce you to return? I need higher study like PhD but I must like to do research and teaching for my country Bangladesh. 6. Any other comments? I am a lecturer from a newly established university. As a man of new university I have a appeal to all

that if there is any chance please try to help us for the development of Fisheries Biology and Genetic Laboratory in my university.

224

Dr Mohammad Shoeb A. Biosketch of Participants:

1. Name with academic title: Dr Mohammad Shoeb 2. Current position with affiliation, address and contact details: Assistant Professor, Department of

Chemistry, University of Dhaka, Dhaka-1000, Bangladesh, E-mail:shoeb712yahoo.com, Phone:017151919188

3. Immediate past position(s) 4. Graduate and postgraduate degrees and training: PhD in Natural Products Chemistry from the Robert

Gordon University, UK and MSc from University of Aberdeen, UK. MSc and BSC in Chemistry, University of Dhaka.

5. Prizes/Awards/Honours (academic and research): Young Chemist Travel Grant Award from Phytochemical Society of Europe

6. Elected Fellowships of Academies 7. Research Advisory Positions/Journal Editorship/Directorship of Boards 8. Membership of Institutional Committees:

Bangladesh Chemical Society, Phytochemical Society of Europe, Asian Network of Research on Antidiabetic Plant materials (ANRAP), NITUB

B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research: Natural Products Chemistry and

Environmental Chemistry 2. Area(s) of Expertise: Natural Products, Analytical Chemistry 3. Number of peer-reviewed publications:25 4. List of 5-10 most significant publications 5. Patents (PCT applications/granted) 6. Competitive Research Grants obtained 7. Size and qualifications of research team supervised: 10 8. National and International Collaborations: National: with Department of Fisheries, Department of Environment, BIRDEM, Bangladesh

National Herbarium, BCSIR 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?): In Our

lab: HPLC, GC, GC-MS, Extraction and Chromatographic Techniques

Poster presenters

225

Md. Rashedul Islam

A. Biosketch of Participants: 1. Name with academic title Md. Rashedul Islam, B.Sc. AH and MS in ABG (BAU) 2. Current position with affiliation, address and contact details

Scientific Officer (SO), Animal Division, National Institute of Biotechnology (NIB), C/O Atomic Energy Research Establishment (AERE), EPZ, Savar, Dhaka-1349, Bangladesh

3. Immediate past position(s): Not applicable 4. Graduate and postgraduate degrees and training

Graduate: Bachelor of Science in Animal Husbandry (B.Sc. AH) Postgraduate: Master of Science in Animal Breeding and Genetics (MS in ABG)

5. Prizes/Awards/Honours (academic and research): Not applicable 6. Elected Fellowships of Academies : Not applicable 7. Research Advisory Positions/Journal Editorship/Directorship of Boards: Not applicable 8. Membership of Institutional Committees Member of GNOBB B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research Reproductive Biotechnology, Mouse embryo culture 2. Area(s) of Expertise Assisted reproductive technology 3. Number of peer-reviewed publications 4. List of 5-10 most significant publications

Islam, M.R., Khandoker, M.A.M.Y., Afroz, S., Rahman, M.G.M. and Khan, R.I. 2005. Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos. MOL BIOL & BIOTECH J. 3 (1&2) Islam, M.R., Khandoker, M.A.M.Y. and Afroz, S. 2005. In vitro maturation, fertilization and subsequent development of goat oocytes. MOL BIOL & BIOTECH J. 3 (1&2) Khandoker, M.A.M.Y., Ali, M.R., Islam, M.R., Rahman, M.G.M. and Talukder, M.A.S. 2005. In vitro culture of mouse embryos. Progress. Agric. 16 (1): 121-128. Talukder, M.A.S., Khandoker, M.A.M.Y., Rahman, M.G.M., Islam, M.R. and Khan, M.A.A. 2005. Reproductive Problems of Cows at Bangladesh Agricultural University Dairy Farm and Possible Remedies. Pakistan J. Biol. Sci. 8(11): 1561-1567. Khandoker, M.A.M.Y., Ali, M.R., Islam, M.R., Rahman, M.G.M. and Talukder, M.A.S. 2005. In vitro culture of mouse embryos. BAU Res. Prog. 16: 34. Proceedings of the workshop held in 29-30 November 2005. Khandoker, M.A.M.Y. and Islam, M.R. 2005. In vitro culture of bovine embryos. BAU Res. Prog. 16: 34. Proceedings of the workshop held in 29-30 November 2005. Khandoker, M.A.M.Y., Afroz, S., Islam, M. R. and Husain, S. S. 2006. Cryopreservation of Black Bengal Buck Semen. P. 526. Proceedings of 12th aaap Animal Science Congress, held in september 18- 22, 2006 in Korea.

5. Patents (PCT applications/granted): Not applicable 6. Competitive Research Grants obtained: Not applicable 7. Size and qualifications of research team supervised: Not applicable 8. National and International Collaborations: Not applicable 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?)

Poster presenters

226

Md. Rashedul Kabir Mondol A. Biosketch of Participants:

1. Name with academic title- Md. Rashedul Kabir Mondol Lecturer 2. Current position with affiliation, address and contact details-

Lecturer Department of Fisheries University of Rajshahi, Rajshahi-6205 Phone: +88-0721-750041-4117 (Office) Fax: +88-0721-750064 Mobile: 01716-446562 E-mail: [email protected]

3. Immediate past position(s)- Research Fellow in the research project entitled “Comparative studies on genetic variability between

wild and hatchery stocks of catla (Catla catla Hamilton) by microsatellite marker and implication of hatchery management practices on its pond performance” funded by SUFFER, Department For International Development (DFID) from 1st February to 31st December 2004 in the Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh.

4. Graduate and postgraduate degrees and training- B. Sc, Fisheries (Honours), Bangladesh Agricultural University M. S. in Fisheries Biology & Genetics, Bangladesh Agricultural University

5. Prizes/Awards/Honours (academic and research)- Not Applicable 6. Elected Fellowships of Academies- Not applicable 7. Research Advisory Positions/Journal Editorship/Directorship of Boards- Not applicable 8. Membership of Institutional Committees- Not applicable B. Research and Research Management Experience: 1. Major project area(s); with very brief description of current research- Not applicable 2. Area(s) of Expertise- Not applicable 3. Number of peer-reviewed publications- Not Applicable 4. List of 5-10 most significant publication- Not Applicable 5. Patents (PCT applications/granted)- Not Applicable 6. Competitive Research Grants obtained- Not Applicable 7. Size and qualifications of research team supervised- Not Applicable 8. National and International Collaborations- Not Applicable 9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?)- Not applicable

Poster presenters

227

Mr. Md. Sazzadur Rahman


1. Name with academic title

Mr. Md. Sazzadur Rahman

1. Current position with affiliation, address and contact details

Scientific Officer, Molecular Plant Physiology (Stress Physiology), Plant Physiology Division, Bangladesh Rice Research Institute (BRRI), Gazipur-1701, Bangladesh.


Not applicable


Education: M.S. in Crop Botany (Appeared) B.Sc. in Agriculture (1st class and placed 10th in merit) Training: 1. Introductory Course in Molecular Biology, Held at BRRI,

Gazipur, 1-5 March 2003. 2. Hybrid Rice Seed production Technology, Held at IRRI, Los

Banos, Philippines, 29 March to 7 April 2004. 3. Foundation Training Course for NARS scientist, Held at

BARD, Comilla, 28th February 2005 to 26th June 2005. 4. Rice Production, Communication and Office Management,

Held at BRRI, Gazipur, 21 December 2003 to 18 February 2004


Karim memorial trust prize for best student at the Department of Agricultural Chemistry in the year 2001 (BAU, Mymensingh). DG’s award 2005 for getting first position at the Foundation Training Course for NARS scientist (12th batch, BARD, Comilla).

4. Elected Fellowships of Academies

1. NST fellow in 2001. 2. Outreach Research fellow 2006.

5. Research Advisory Positions/Journal Editorship/Directorship of Boards

Not applicable


Office Secretary of the BRRI Scientist Association (BRRISA)


1. Major project area(s); with very brief description of current research

“Marker Assisted Backcrossing for salinity tolerance of rice” which is under the project of Generation Challenge Program (GCP).

2. Area(s) of Expertise Stress Physiology of rice and Physiology of rice plant nutrition.

Poster presenters

228

3. Number of peer-reviewed publications

Not applicable.


a. Mahbub, A.A., Sazzadur Rahman, M. Khanam, M. and Gomosta, A.R. 2005. Development of Preharvest sprouting tolerance screening technique in rice. IRRN 30(1):50-51.

b. Mahbub, A.A., Khanam, M., Rahman, M.S., Hossain, M.A. and Gomosta, A.R. 2006. Determination of lodging characters of some BRRI recommended rice varieties at three nitrogen levels during wet season in Bangladesh. Bangladesh J. Bot. 35(2):117-124.

c. Rahman, M.S., Khanam, M., Mahbub, A.A., Gomosta, A.R. and Islam, M.S. 2007. Effects of a growth retardant Multi-Effects-Triazole (MET) on lodging of BRRI dhan32. IJBR-35 (Press).

d. Rahman, M.S., Khanam, M., Rashid Sorker, A.S.M.H., Aurangozeb, M.K. and Islam, M.S. 2007. Effect of Multi-Effect Triazole (MET) on the growth and yield of BRRI dhan32. IJBR-41 (Press).

e. Khanam, M., Rahman, M.S., Mahbub, A.A., Yeasa, M. and Gomosta, A.R. 2007. Growth efficiency of rice. Bangladesh J. Bot. 36 (press).

5. Patents (PCT applications/granted)

Not applicable.

6. Competitive Research Grants obtained

Not applicable.

7. Size and qualifications of research team supervised

Not applicable.

8. National and International Collaborations

Not applicable.

9. Major Equipment/Facilities in Bangladesh (will these be available to other researchers?)

Not applicable.

229

Dr. S. M. Shahinul Islam A. Biosketch of Participants:

1. Name with academic title

Dr. S. M. Shahinul Islam

2. Current position with affiliation, address and contact details:

Assistant Professor, Plant Biotechnology Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh


Assistant Director (Research Section), BOU

4. Graduate and Postgraduate degrees and training

Graduate: B. Sc (Hons) Botany

Postgraduate: M. Sc (Botany), Ph.D (Plant Biotechnology), Post-doctorate (Genetic Engineering)

Training:

Plant/Microarray Hybridization Workshop (July 11, 2005 - July 15, 2005) .Organized by The Institute of Genomic Research (TIGR), Rockville, MD, USA.

Concepts and Techniques in Plant Cell and Molecular Biology. Organized by Friedrich Miescher Institute (FMI), Basel, Switzerland (October 14-25, 1996).

5. Prizes/Awards/Honours (academic

and research)

i) Awarded the Swiss Federal Commission of Scholarships for Foreign Students (ESKAS, Bern) for Two years (15th July 1995 – 25th July 1997) and then Four months 15th March – 15th July 1999 (Switzerland).

ii) Awarded The Japan Society for the Promotion of Science (JSPS) Fellowship for Postdoctoral research in Japan (October 2003 to October 2005- Two years).

iii) Fellowship awarded by the Ministry of Science and Technology (NST), Govt. of Bangladesh, Bangladesh for post-graduate research (2000).

6. Elected Fellowships of Academics

Not applicable

7. Research Advisory Positions/Journal Editorship/Directorship of Boards

Not applicable


American Society of Plant Biologists (ASPB)- Member Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)- Member Bangladesh Association of Plant Tissue Culture and Biotechnology (BAPTC& B)- Life Member Bangladesh Botanical Society-Life Member

Poster presenters

230


1. Major project area(s); with brief description of current research

Not applicable

2. Area(s) of Expertise Plant Biotechnology

3. Number of peer-reviewed publications Not applicable

4.

5. Patents (PCT applications/granted) Not applicable

6. Competitive Research Grants obtained Not applicable

7. Size and qualifications of research team supervised

Not applicable

8. National and International Collaborations Not yet

9. Major Equipments/Facilities in Bangladesh (will these be available to other researchers?)

PCR Machine, Horizontal and vertical gel electrophoresis, Florescence Microscope, Microtome Machine, Laminar Air Flow, Ultrasonic Cell Disintrigator, Rotary Vacuum evaporator, etc.

231

Conference

Committees

Chief Patrons Professor Dr. S.M.A. Faiz, [email protected]

VC, Dhaka University (DU) Professor Dr. J. R. Choudhury [email protected]

VC, BRAC University Patrons Professor Dr. Yusuf Haider [email protected] ProVC, Dhaka University

Dr. Salehuddin Ahmed [email protected] ProVC, BRAC University

Advisors Mr. M. Syeduzzaman [email protected] Chairman. Bank Asia and BRF

232

Dr. A. A. Azad [email protected]

TWAS Fellow & Research Professor Prof. Dr. M. Abdul Aziz [email protected] Project Director, NIB

Dr. Abed Chaudhury [email protected] CSIRO, Australia

Steering Committee

Convener Prof. Dr. A. S. Islam [email protected] Scientist, UT and GNOBB moderator Member Secretary Prof. Dr. N. Choudhury [email protected]

Sec. BAS & Coordtr Biotech, BU Members Mr. Tapan Choudhury [email protected] Managing Director Square Pharmaceuticals Prof. Harun Yusuf [email protected] Biochemistry & Molecular Biology

Dhaka University President, Bangladesh Biochemical.Society Prof. Mustafizur Rahman [email protected] Biochemistry & Molecular Biology, Dhaka University Professor Dr. Saleheen Qadri [email protected] Biochemistry & Molecular Biology, Dhaka University VP, Bangladesh Biochemical Society Prof. Khairul Bashar, [email protected] Pro VC, North South University Prof. Dr. Ziauddin Ahmed [email protected] Biochemistry & Molecular Biology, Jahangir Nagar University Dr. M.A. Hossain [email protected] Prof. Biochemistry & Molecular Biology Dean, Biological Science Faculty

233

Dhaka University Dr. S. M. Faruque [email protected] Scientist, Molecular Genetics, ICDDR,B Prof. Dr. M.A. Rashid [email protected] Dean, Pharmacy, Dhaka University Prof. Dr. Serajul Islam [email protected] VC , Jagannath University Prof. Bahadur Meah [email protected] Plant Pathology, Bangladesh Agricultural University Prof. M. Rahmatullah [email protected] Dean Life Science. University of Development Alternative Mr. Abdul Muktadir [email protected] MD, Incepta Pharmaceuticals Ashfaque Hossain [email protected] U.S.A Dr. Liaquat Ali [email protected] Prof. Biochemistry & Cell Biology., BIRDEM, Dhaka Prof. Dr. S.K. Bhadra [email protected]

Botany, Chittagong University Dr. Khan Shahidul Haque [email protected] Chief Scientific Officer, BLRI Dr. Sayedul Islam [email protected] Prof. Biochemistry & Molecular Biology,

Dhaka University Dr. Sharif Akhteruzzaman, [email protected] DNA profiling Laboratory., Dhaka Medical College Hospital Ex-Officio members Advisors, Member-secretaries of OC, convener and co-convener of OC

Financial Committee

234

Convener Professor Dr. Saleheen Qadri [email protected] Director, Center of Excellence, Dhaka University

Dr. Zakir H. Howlader [email protected] Member-Secretary Biochemistry and Molecular biology, DU Members Dr. Sultanul Aziz [email protected] former Associate. Director, ICDDR,B Dr. Jalaluddin Bhuiyan [email protected] King Faisal Hosp and Res. Mr. Abdul Muktadir [email protected] MD, Incepta Pharmaceuticals Mr. Muhammadul Haque [email protected] Director Marketing Mr. Rafiqul Islam [email protected] Marketing Dir., ACME Pharma

Dr. Abdur Razzaque [email protected] Member Crop

BARC

Dr. Abul Kalam Azad [email protected] Professor, Biochemistry, NICD

Mr. Munir Hasan [email protected] Natl. P. Coortr., ICT Capacity of PMO

Dr. Samir Saha [email protected] Prof. and Sr. consultant, Dhaka Shishu Hosp. Dr. Mozammel Hoq [email protected] Professor, Micrbiology, Dhaka University

Ms. Momtaz Faruki [email protected] Free-lance Conlt Agri. Persps.

Dr. Nasiruddin [email protected] Bangladesh Agriculture

University , Mymensingh Dr. Mamun Ahmed [email protected] Biochemistry & Molecular Biology

Dhaka University

235

Dr. Emran K. Choudhury [email protected] Biochemistry & Molecular Biology Dhaka University Ex-officio members Advisors, Convener, Co-conveners and member secretaries, OC and SC.

Scientific Committee Convener Dr. Firdausi Qadri, [email protected] Senior. Scientist and Head, Immunology, ICDDR,B

Dr. Mohammad Arif, [email protected] Member-Secretary Biochemistry & Molecular Biology, Dhaka University. Members Professor Dr. Rafiqur Rahman [email protected]

Biochemistry and Molecular Biology, Dhaka University. Prof. Dr. Laila N. Islam [email protected] Biochemistry and Molecular Biology, Dhaka University. Dr. Matiur Rahman [email protected] ICDDR,B Prof. Dr. Ishtiaq Mahmud [email protected] Biochemistry and Molecular Biology, Dhaka University.. Prof. Dr. Mamum R. Choudhury Biochemistry and Molecular Biology, Dhaka University. Dr. Ahmed Ashraf [email protected] Dr USAMRIID Dr. Nur e Kamal

[email protected]

Dr. Abu Siddique [email protected]

Dr. Lamia Sharmeen [email protected] Dr. ATM Shamsul Hoque [email protected] Sc.D., Sanofi Aventis Prof. Dr. C. Rafiqul Ahsan [email protected] Microbiology, Dhaka University.

236

Dr. Parvez Haris [email protected] Dr. Hemayet Ullah [email protected] Dr. Abul Ekramuddoullah [email protected] Dr. Kamal Choudhury [email protected] Dr. Zaheed Hosain [email protected] Dr. Zahid Hossain [email protected] Dr. Mir Firoz Ahmad [email protected] Dr. Reza Haque [email protected] Dr. Abidur Rahman [email protected] Prof. Dr. Anwar Hossain [email protected] Microbiology, Dhaka University. Prof. Dr. Apala F. Naved [email protected]

Biochemistry and Molecular Biology, DU Prof. Dr. Aftab Uddin, [email protected] Biochemistry and Molecular Biology, DU Dr. Jesmin [email protected] Genetic Engineering and Biotechnology, Dhaka University. Ex-officio members Advisors, Convener, Co-conveners and member secretaries, OC and SC

Organizing Committee Convener Prof. Dr. Yusuf Haider [email protected] ProVC, Dhaka University Co-Convener Professor Dr. Saleheen Qadri [email protected] Director, Center of Excellence Dhaka University Member-secretaries Dr. Zeba I. Seraj [email protected] Prof. Biochemistry & Molecular Biology Dhaka University Dr. Haseena Khan [email protected]

237

Prof. Biochemistry & Molecular Biology Dhaka University Members: Mr. Rafiqul Islam [email protected] Marketing Dir., ACME Pharma Dr. Abdur Razzaque [email protected] Member Crop, BARC Dr. Mozammel Hoq [email protected] Prof. Micrbiology Dhaka University Dr. S. M. Faruque [email protected] Scientist, Molecular Genetics ICDDR,B Dr. R. H. Sarker [email protected] Prof, Botany Dhaka University Dr. Imdadul Hoque [email protected] Prof, Botany Dhaka University Dr. Raihan Ali [email protected] Head, Biotechnology discipline, Khulna University Dr. Yousuf Islam [email protected] Prof. CS & Res. Director, BRAC University Dr. M. Shahjahan [email protected] Prof. Biochemistry Rajshahi University Dr. Samir Saha [email protected] Prof. and Senior. consultant, Dhaka Shishu Hospital. Mr. Abdul Muktadir [email protected] MD, Incepta Pharmaceuticals Mr. Muhammadul Haque [email protected] Director Marketing

238

Dr. Abul Kalam Azad [email protected] Prof, Biochemistry, NICD Mr. Munir Hasan [email protected] Natl. P. Coortr., ICT Capacity of PMO Dr. A. A. Akhand [email protected] Chairman, Genetic Engineering & Biotechnology , Dhaka University Dr. Nurun Nahar [email protected] Scientist, DNA laboratory, Center of Excellence, Dhaka University Dr. Aparna Islam [email protected] Assistant Professor, MS, Biotechnology BRAC University Mr. Mustak Ibn Ayub [email protected]

Representative Young BB group

Ex-Officio members Advisors, convener and member secretary, SC

Organizing Assistants Conference Secretariat

Shamim Reza Nazlee Sharmin

MS, Biochemistry and Molecular Biology MS, Genetic Engineering and Biotechnology

Lisa Perveen Miraj Kobad Choudhury

MS, Biochemistry and Molecular Biology MS, Genetic Engineering and Biotechnology

Richard Malo S.M. Mahbubur Rashid

239

MS, Biochemistry and Molecular Biology 4th year, Genetic Engineering and Biotechnology

Shakhinul Islam Mondol Md. Mehedi Hasan


Sharmin Jahan Salim Ahmed


Rejbana Alam Waise Quarni

MS. Biochemistry and Molecular Biology 4th year, Genetic Engineering and Biotechnology

Sabrina Moriom Elias Sarmah Bin Nayeem

4th year, Biochemistry and Molecular Biology 4th year, Genetic Engineering and Biotechnology

Sadia Nawraz Khan Tanzila Mahzabin

4th year, Genetic Engineering and Biotechnology


Mustak Ibn Ayub Nusrat Sharmeen


3rd year, Genetic Engineering and Biotechnology

A Hoque , 57 A K Mattoo, 92 A. A. Chowdhury, 42 A. H. Khan , 48, 97 A. Hoque, 58 A. Jittmittraphap, 97 A. K. Azad Chowdhury , 22,81,129,137 A. K. Azad Khan, 125 A. K. M. Shahidur Rahman, 22 A. Komamine , 41 A. Siddika , 136 A. Tanaka A H Ide, 50 A.F.M. Jamal Uddin, 38 A.K.M. Shahidur Rahman , 81 A.K.Saha, 54

Abdul Quyyum Khan, 82 Abdullah Al Mueen, 52 Abed Chaudhury , 133 Abul K M Ekramoddoullah, 28 Ahmad Humayan Kabir , 83 Ahmad S. Islam , 84 Ahmed A Azad, 19

Akihiro Takeuchi, 26 Alam Nur-E-Kamal , 20 Aleya Awal, 27 Ali Azam Talukder , 85 Aliya Ferdousi, 131 And J.F. Prescott , 124 Anne-Dominique Lajoix, 114 Anwarul Azim Akhand, 47 Anwarul karim, 45

Apala Farhat Naved, 44 Aparna Islam , 37 Aryati, 97 B. C. Sarker, 33 B.B. Roy , 56 B.C.Y. Collard, 40 Bo L Lonnerdal., 46 Chieko Wada, 51 D. A. N. Majumder, 33 D.L. Adorada, 40 David Sack., 45 Dinesh Mondal, 45 Dominique Bataille, 114 Dr. A. K. Azad, 66 Dr. Anwar Nasim, 62 Dr. Jalaluddin Bhuiyan, 67

INDEX

240

Dr. Liaquat Ali , 71 Dr. Md. Abdur Razzaque, 68 Dr. Md. Mukhlesur Rahman Khan, 55 Dr. Sharif Akhteruzzaman, 70 Dung Lenguyen, 114 E H Emran, 126 E. J. Garvey, 104,35,105 E. Tumimbang-Raiz, 40 E.H.M.S. Rahaman, 105 Eiichi Tamiya, 26 El Habib Hani., 114 Eric Vives, 114 F. Hasebe , 48 F. Islam, 33 F. Okamoto, 91 Fahima Chowdhury, 45 Farzana Marni, 137 Fumio Arisaka , 139 G. A. Hitman , 125 G.B Gregorio, 39,40 Gary Adams, 49 Gazi Nurun Nahar, 133 Golam Hasan Rabbani, 22,137 H Islam , 126 H. Hashimoto, 91 H. Morishima., 41 H. R. M. Masud Anwar, 33 Habibul Bari Sozib, 131 Halima Sadia Khan , 86 Haseena Khan, 27 Hirotada Mori, 51 Hosne Ara Ali , 81 Imran Parvez, 87 Izumi Nakashima, 47 J. Matthew Taliaferro, 84 J.P.W. Young , 106 J.W. Choi and K.S. Ryu , 102 Jalaluddin Bhuiyan , 88 Jan Andersson, 45 Jesmin , 89 K Farhana, 126 K H M Nazmul Hussain Nazir, 91 K Hoshinoo, 50 K. Azad, 125 K. B. Biswas, 125 K. Furukawa, 91 K. Kageyama, 38 K. Morita , 48,97 K. Nagamiya, 41 K. Nakao, 41 K. Nishimura, 42 K. S. Huque , 53 K. Sathasivan, 84 K. T. Osman , 113 K. Yokota, 42 K.M. Hossain , 56 Kagan Kerman, 26 Kaisar Ali Talukder, 22,98 Kazi Asraful Alam , 90 Khaled Hossain , 47 KM Nasiruddin, 92

L. Ali, 125,130 L. Hassan, 40 Laisa Ahmed Lisa, 93 Lamia Sharmeen, 21 Liaquat Ali, 114 Lisa Parvin, 134 Lutful Hassan , 36 Lutfur Rahman, 34,95,119,122 M A Hasanat, 94 M. A. Sattar 106 M. A. Hossain, 113 M. A. Kabir, 33 M. A. Maya, 104 M. A. Mazid, 42,72 M. A. Rahim, 33 M. A. Rahman , 35 M. A. Rashid, 42,72 M. Alimul Islam S. Inoue, 97 M. Anwar Hossain, 26 M. Asaduzzaman, 22 M. Azizur Rahman, 109 M. Bahadur Meah , 101 M. C. Parquet , 48 M. Firoz Alam, 31 M. Goto , 91 M. Hossain, 57,58 M. I. Hawa, 125 M. I. Hoque, 30 M. Jisaka, 42 M. Khalekuzzaman, 31 M. M. Islam, 136 M. Mahfuzur Rahman , 109 M. Moshiuzzaman , 123 M. N. Anwar, 113 M. N. Sultana, 108 M. O. Faruque, 125 M. R. Haque, 72 M. R. Hassan, 108 M. S. Alam, 33 M. S. Bari, 33 M. S. Rahman, 72 M. Sawkat Ali, 81 M. Senda, 38 M. U. Ahmed , 48 M.A. Alim , 115 M.A. Azam , 82 M.A. Islam, 40,48 M.A.I. Talukder; , 56 M.A.M.Y. Khandoker , 117 M.A.Mannan , 54 M.A.N.Nazim-Ud-Dowla, 95 M.G. Rabbani, 35,105 M.K. Mahanta , 54 M.M. Islam , 39,82 M.R. Anower , 116 M.R. Islam, 40 M.S. Haque, 82 M.S.Alam , 34 M.S.R. Khan, 50 M.Shah-e-Alam, 34 Mafizul Islam , 110 Mahbubur Rahman , 22,129 Mahzabin Amin, 132

Maki Maeda, 51 MB. Ahmed, 58 Md Abdul Khaleque , 96 Md Maksudul Alam, 121 Md. Asaduzzaman, 98 Md. Ashraful Islam Bhuiya , 99 Md. Azizur Rahman , 100 Md. Belal Hossain, 102 Md. Ekramul Hoque , 103 Md. Golam Rabbani , 104 Md. Harun-or Rashid, 106 Md. Israque Hossain Ansari , 107 Md. Jamal Uddin, 108 Md. Maksudul Alam , 52 Md. Mukhlesur Rahman Khan , 87 Md. Munan Shaik , 111 Md. Munan Shaik , 112 Md. Nazim-ud-Dowla , 34 Md. Omar Faruque , 114 Md. Omar Faruque, 115 Md. Rashedul Islam, 117 Md. Rashedul Kabir Mondol, 118 Md. Rezwan Molla , 122 Md. Rezwan Molla, 119 Md. Rezwan Molla, 95 Md. Saidur Rahman, 52 Md. Samsul Alam , 119 Md. Samsul Alam , 95 Md. Samsul Alam, 118 Md. Sazzadur Rahman , 120 Md. Shahidul Islam , 118 Md. Shakhinur Islam, 121 Md. Shamsuzzoha Bayzid, 52 Md. Shawkat Ali , 137 Md. Shefatur Rahman, 119 Md. Shefatur Rahman, 122 Md. Shefatur Rahman, 34 Md. Shefatur Rahman, 95 Md. Shoeb, 123 Md. Tanvir Rahman, 124 Md. Zahid Hassan , 125 Mehmet Ozturk, 63

MK Biswas, 57 MK. Biswas , 58 Mohamad W. Wazni, 84 Mohammad Arifuzzaman, 51 Mohammad Bakhtiar Hossain, 46 Mohammad Nazrul Islam, 119 Mohammad Nazrul Islam, 34 Mohammad Nazrul Islam, 95 Mohammad Shawkat Ali, 22 Monira Obaid, 27 Mr.Michael Behan, 69 N Islam, 126 N Nahar, 57 N. A. Chowdhury , 48 N. Begum , 48 N. Nahar, 123 N. Talemaitoga, 97 N.Naher, 33

241

Nadim Ashraf , 49 Nadim Ashraf, 27 Nadira Islam , 127 Nahid Akhter, 22 Naoki Nagatani, 26 Nasrin Sultana, 53 Nazlee Sharmin, 121 Nesar Uddin Ahmed, 34 Nilufar Yasmin Shaikh , 128 Nishat Nasreen, 22 Nishat Nasrin , 129 Noorain Munim Rasul, 134 Philippe Herve, 31 Pierre Petit, 114 Prof Asma Ismail, 64 Prof. A. K. Azad Chowdhury , 98 Prof. Ahmed A. Azad, 65 Prof. Virander Singh Chauhan, 61 R Begum, 92 R Islam, 57 R. D. G. Leslie, 125 R. H. Sarker, 30 R. Islam, 125 R. Islam, 58 R. Karim, 130 R. Karim, 57 R. M. Emon, 136 R.D. Mendoza, 40 R.M. Emon, 82 Rahelee Zinat, 130 Rakibul Islam, 132 Rashidul Haque, 43 Rehana Hashem , 30

Rejbana Alam, 131 Rezwan Mollah, 34 Richard Malo, 132 Rintarou Saito, 51 Rokeya Begum, 133 Rokeya Sultana, 27 Rubhana Raqib, 45 Rumana Sultana Tammi, 134 S K Sopory , 92 S. Afroz, 117 S. Basak , 108 S. Bipolo , 48 S. Inoue , 48 S. Islam, 130 S. Jesmin, 41 S. M. Shahinul Islam et al , 135 S. Mahfuja Khatun, 31 S. N. Begum, 136 S. Sultana, 136 S. Uematsu, 38 S.A. Aziz, 115 S.K. Talukder, 36 S.N. Begum, 82 Saaimatul Hoque, 27 Salil Kumar Bhowmik , 136 Samir K. Saha, 73 Samiul Haque, 27 Sanaul Bashar, 45 Sayeda Sulata, 34 Sazzadur Rahman , 131 Sazzadur Rahman, 93 Shahani Noor, 89 Shakinur Islam Mondal , 27 Shamsul H. Prodhan, 41 Shannon L Kelleher, 46

Sharmin Jahan, 134 Sheikh Zahir Raihan, 137 Sheikh Zahir Raihan, 22 Shigehiko Kanaya, 51 Shuji Kanamaru, 139 Soheli Sattar , 138 Stephen Luby, 45 Subodh Kumar Sarkar, 139 Suhaila Rahman, 131 Sultan Ahmed, 45 Swapan K. Datta , 31 T. Futagami, 91 T. Motohashi, 41 T. Nabeshima, 97 T. Nagaya, 42 Taher Uddin , 140 Taiji Adachi, 128 Takao Kondo, 85 Tasnim Azim , 141 Tatsuro Endo, 26 Teruko Yuhi, 26 U.K. Roy, 57 V. S. Reddy, 37 V.M. Nicholson, 124 Y Hanafusa , 50 Y Tagawa2, 50 Y. Jin, 97 Y. Suzuki , 48 Yuzuru Takamura , 26 Zeba I Seraj , 2s9,93 ,131, 132, 134

Abstract Book

Documents

day conference

biotechnology policy

research icddr

private institutes

promotion of biotechnology

national development

scientists unable

private universities