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01402311 ชีวเคมี I - Pirun Web faasptps/01402311.pdf · PDF file · 2011-11-21•...

Mar 20, 2018

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  • 01402311 I

    Biochemistry I

  • 01402311 I (Biochemistry I) 2(2-0)

    : 01403221 01403223

    Cells and cell components; structure and functions of water in cellular

    biochemical processes; buffer solutions; structure, properties, functions of

    carbohydrates, proteins, nucleic acids, lipids, enzymes and coenzymes; and

    applications.

  • (3 ) . 11%

    (5 ) . 17%

    (5 ) . 17%

    (4 ) . 13%

    (5 ) . 16%

    (5 ) . 16%

  • ?

  • 1. (Molecular level)

    Carbohydrate,

    Lipid, Protein, Nucleic acid, Vitamin, Hormone

    2. (Metabolism level)

    (Anabolism) (Catabolism)

    3. (Regulatory level)

    0140231101402311 I I

  • Wohler

    Buchners

    Sumner

    Flemming

    Mendel

    Meischer

    Watson Crick

  • ..1828: Wohler

    Friedrich Whler

    The first organic compound to be synthesised from

    inorganic starting materials.

    "I must tell you that I can make urea without the use of kidneys, either man or dog.

    Ammonium cyanate is urea."

    This synthesis undermined the Vitalism Theory, by showing that

    organic compounds could be synthesized from inorganic materials.

  • Buchners

    The winner of the 1907 Nobel Prize

    in Chemistry for his work on fermentation.

    EduardEduard BuchnerBuchner

  • Sumner

    James BJames B. . Sumner Sumner

  • Flemming

    German anatomist Walter Flemming recognized and explored the fibrous network within the nucleus, which he termed chromatin. (Flemming

    had actually discovered the chromosome)

    Drawing of mitosis by Walther Flemming. (F. C. W. Vogel, Leipzig, 1882).Reproduced from Flemming, W. Zellsubstanz, Kern und Zelltheilung

  • Mendel

  • Meischer

    Friedrich Miescher, The man who discover DNA.

    Miescher carried out for the isolation, analysis and

    characterization of DNA.

    Johannes Friedrich Johannes Friedrich MiescherMiescher

  • Watson Crick

    James Watson and Francis Crick,James Watson and Francis Crick,

    crackers of the DNA code, in 1959crackers of the DNA code, in 1959

  • Biophysics

  • 1. C,H,O,N,P

    2.

    3.

  • (Prokaryotic)

    - DNA

  • 1. prokaryotic cell 2. eukaryotic cell

    Kingdom Monera

    (, )

    ,

    nucleoid

    organelles

    Kingdom Protista (,

    )

    Kingdom Fungi (, , )

    Plantae (, )

    Animalia ()

    ,

    cytoplasm cytosol

    organelles

  • http://www.cellsalive.com/cells/cell_model.htm

    Cell Animation

  • A plant cell

  • An animal cell

  • ?

    - - - -

  • H

  • hydrophilic

    -

    -

    The coating also has a hydrophilic property that makes water droplets spread out, or sheet, across the surface of the glass.

  • hydrophobic

    -

    -

    -

  • amphipathic

    - hydrophilic hydrophobic

    - detergents ( )

  • amphipathic

  • Membrane Structure

  • Na+ O O

    O : O

    H H

    Cl- H

  • : OH

    ()

  • physiological pH

    pH pH

    physiological pH pH 6.8-8.0 pH

    bicarbonate phosphate

  • http://www.waterontheweb.org/under/waterquality/pH.html

  • Henderson-Hasselbalch

    pH = pKa + log

    pH

    [A-][HA]

  • pH pKa

    pH 1

    pKa

    Henderson-Hasselbalch

  • pH 4.00

    pKa 4.00 (3.75)

    (pKa = 4.76)

    0.1 M sodium formate

    pH = 4.00 (Ans. = 0.178 M)

    0.1 M pH 4.00 NaOH

  • The endThe end

  • Blog Biochem

    www.gotoknow.org/blog/biochem

  • I (20 10 %) 1. . collagen . benzene

    . -amylase . cellulose 2. cell membrane . phospholipids . peptidoglycan

    . glycoside . lignin 3. Whler . ammonium cyanate . ribose

    . urea . ethanol 4. Buchner . .

    . . ethanol 5. Meischer . DNA .

    . phospholipids . ATP 6. urease Sumner . . . . 7. 100 nm ( 2 mm) . 1,000 . 2,000

    . 10,000 . 20,000 8. . 1 m . 1 nm

    . 0.1 mm . 1 9. prokaryote . ribosome . cell membrane

    . nuclear membrane . cell wall 10. . cell wall . lysosome

    . vacuole . chloroplast 11. organelle organelle . cell membrane . ribosome

    . Golgi complex . lysosome 12. organelle . endoplasmic reticulum . ribosome

    . chloroplast . mitochondria 13. carbohydrate . cell wall . cell membrane

    . cell membrane fungi . cell membrane 14. mycoplasma . nucleus . ribosome

    . cell wall . genome 15. amphipathic . palmitic acid . acetic acid

    . galactose .

  • 16. . .

    . . 17. . fructose .

    . phospholipids . ethanol 18. . .

    . . 19. pH physiological pH . .

    . . 20. . pH .

    . .

  • I ______________________________ ___________________ 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 X X X X X X X X X X X X X X X X X X X X

    01402311 I ?..1828: Wohler Buchners Sumner Flemming Mendel Meischer Watson Crick (Prokaryotic) H hydrophilic hydrophobic amphipathic amphipathicMembrane Structure physiological pH Henderson-Hasselbalch pH 4.00The endBlog Biochem