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科学研究費助成事業 研究成果報告書
様 式 C-19、F-19、Z-19 (共通)
機関番号:
研究種目:
課題番号:
研究課題名(和文)
研究代表者
研究課題名(英文)
交付決定額(研究期間全体):(直接経費)
14401
挑戦的萌芽研究
2015~2014
化合物スクリーニングによる子宮頸癌の癌幹細胞を標的とした新規放射線増感剤の探索
Identification of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening
研究成果の概要(英文):Cancer recurrence and metastases can be due to a subpopulation of cells with stem-like characteristics, cancer stem cells (CSCs) which maintain the capacity to regenerate entire tumors. We suspected that CSC-specific compounds had radiosensitizing effects in parental cells. To accomplish this purpose, we performed high throughput screening (HTS) to identify compounds that specifically act CSCs. Finally, we found five CSC-specific compounds. However, these compounds had no significant radiosensitizing effects for parental HeLa cells. We also performed cell-based high throughput screening to study new radiosensitizers targeting hypoxic tumor cells. We identified 22 compounds as candidate. Clonogenic survival assay showed their significant radiosensitizing effect in hypoxic condition. In conclusion, we identified new radiosensitizers by high throughput screening. This is a possible method for identifying radiosensitizers targeting hypoxic cancer cells.
①Tamari KKawamoto K, Koseki J, Beak SJ, Taketo K, Doki Y, Mori M, of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening. 名古屋国際会議場、第総会、②Tamari KIsohashi FIdentification of novel radiosensitizers targeting hypoxic cancer cells by high throughput screening. Poster presentation in Tenth AACRBreaktBiology to Therapeutics, Feb 2016, Hyatt Regency Maui Maui, Hawaii, USA.③Tamari KIsohashi Fnovel method to identify radiosensitizers targeting hypoxic cancer celscreening. Poster presentation in AACR Annual Meeting 2016, Apr. 2016, Ernest N. Morial Convention Center New Orleans, Louisiana, USA 〔その他〕ホームページ等 6.(1) 玉利慶介大阪大学・医学系研究科・特任助教
研究者番号: (2) 瀬尾雄二大阪大学・医学系研究科・
研究者番号: 礒橋文明大阪大学・医学系研究科・特任准教授勤
研究者番号: 小川和彦大阪大学・医学系研究科・教授
研究者番号: 林和彦大阪大学・医学系研究科・
研究者番号:
Tamari K, Ishii HKawamoto K, Koseki J, Beak SJ, Taketo K, Doki Y, Mori M, of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening. 名古屋国際会議場、第総会、2015 年Tamari K, Seo Y
Isohashi F, Yoshioka Y, and Identification of novel radiosensitizers targeting hypoxic cancer cells by high throughput screening. Poster presentation in Tenth AACR-JCA Joint Conference on Breakthroughs in Cancer Research: From Biology to Therapeutics, Feb 2016, Hyatt Regency Maui Maui, Hawaii, USA.Tamari K, Seo Y
Isohashi F, Yoshioka Y, and novel method to identify radiosensitizers targeting hypoxic cancer cells by high throughput screening. Poster presentation in AACR Annual Meeting 2016, Apr. 2016, Ernest N. Morial Convention Center New Orleans, Louisiana, USA
〔その他〕 ホームページ等
6.研究組織 (1)研究代表者
玉利慶介 (大阪大学・医学系研究科・特任助教研究者番号:
)研究分担者瀬尾雄二 大阪大学・医学系研究科・研究者番号:
礒橋文明 大阪大学・医学系研究科・特任准教授勤) 研究者番号:
小川和彦 大阪大学・医学系研究科・教授研究者番号:
林和彦 (大阪大学・医学系研究科・研究者番号:
Ishii H, Konno M, Nishida N, Kawamoto K, Koseki J, Beak SJ, Taketo K, Doki Y, Mori M, Ogawa K. Identification of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening. 名古屋国際会議場、第 74 回日本癌学会学術
年 10 月 Seo Y, Takahashi Y, Suzuki O, , Yoshioka Y, and
Identification of novel radiosensitizers targeting hypoxic cancer cells by high throughput screening. Poster presentation in Tenth
JCA Joint Conference on hroughs in Cancer Research: From
Biology to Therapeutics, Feb 2016, Hyatt Regency Maui Maui, Hawaii, USA.
Seo Y, Takahashi Y, Suzuki O, , Yoshioka Y, and
novel method to identify radiosensitizers targeting hypoxic
ls by high throughput screening. Poster presentation in AACR Annual Meeting 2016, Apr. 2016, Ernest N. Morial Convention Center New Orleans, Louisiana, USA
ホームページ等
研究代表者
( TAMARI Keisuke大阪大学・医学系研究科・特任助教研究者番号:30718995
研究分担者 ( SEO Yuji
大阪大学・医学系研究科・研究者番号: 00302000
( ISOHASHI Fumiaki大阪大学・医学系研究科・特任准教授
研究者番号: 00448036
( OGAWA Kazuhiko大阪大学・医学系研究科・教授研究者番号: 40253984
( HAYASHI Kazuhiko大阪大学・医学系研究科・研究者番号: 70718981
, Konno M, Nishida N, Kawamoto K, Koseki J, Beak SJ, Taketo K,
. Identification of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening. ポスター発表、
回日本癌学会学術
, Takahashi Y, Suzuki O, , Yoshioka Y, and Ogawa K
Identification of novel radiosensitizers targeting hypoxic cancer cells by high throughput screening. Poster presentation in Tenth
JCA Joint Conference on hroughs in Cancer Research: From
Biology to Therapeutics, Feb 2016, Hyatt Regency Maui Maui, Hawaii, USA.
, Takahashi Y, Suzuki O, , Yoshioka Y, and Ogawa K
novel method to identify radiosensitizers targeting hypoxic
ls by high throughput screening. Poster presentation in AACR Annual Meeting 2016, Apr. 2016, Ernest N. Morial Convention Center New Orleans,
TAMARI Keisuke 大阪大学・医学系研究科・特任助教(常勤
SEO Yuji ) 大阪大学・医学系研究科・特任助教(常勤
ISOHASHI Fumiaki大阪大学・医学系研究科・特任准教授
OGAWA Kazuhiko 大阪大学・医学系研究科・教授
HAYASHI Kazuhiko 大阪大学・医学系研究科・招へい教員
, Konno M, Nishida N, Kawamoto K, Koseki J, Beak SJ, Taketo K,
. Identification of radiosensitizers targeting cancer stem cells in cervical cancer by using
ポスター発表、回日本癌学会学術
, Takahashi Y, Suzuki O, Ogawa K.
Identification of novel radiosensitizers targeting hypoxic cancer cells by high throughput screening. Poster presentation in Tenth
JCA Joint Conference on hroughs in Cancer Research: From
Biology to Therapeutics, Feb 2016, Hyatt
, Takahashi Y, Suzuki O, Ogawa K. A
novel method to identify radiosensitizers targeting hypoxic
ls by high throughput screening. Poster presentation in AACR Annual Meeting 2016, Apr. 2016, Ernest N. Morial Convention Center New Orleans,